##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934225.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1246121 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30112886308793 33.0 31.0 34.0 31.0 34.0 2 32.477961610469606 34.0 31.0 34.0 31.0 34.0 3 32.53727206266486 34.0 31.0 34.0 31.0 34.0 4 36.02741146325277 37.0 35.0 37.0 35.0 37.0 5 35.14513919595288 37.0 35.0 37.0 35.0 37.0 6 26.132976653150056 36.0 0.0 37.0 0.0 37.0 7 31.12241989341324 37.0 19.0 37.0 17.0 37.0 8 34.766187232219025 37.0 32.0 37.0 32.0 37.0 9 37.29911782242655 39.0 37.0 39.0 35.0 39.0 10 37.290337776187066 39.0 37.0 39.0 34.0 39.0 11 37.326256438981446 39.0 37.0 39.0 34.0 39.0 12 37.157618722419414 39.0 37.0 39.0 34.0 39.0 13 37.19642153530837 39.0 37.0 39.0 34.0 39.0 14 38.3177179423186 40.0 38.0 41.0 34.0 41.0 15 38.464275941100425 40.0 38.0 41.0 34.0 41.0 16 38.531206841069206 40.0 38.0 41.0 34.0 41.0 17 38.524703459776376 40.0 38.0 41.0 34.0 41.0 18 38.48541112781182 40.0 38.0 41.0 34.0 41.0 19 38.50574623170623 40.0 38.0 41.0 34.0 41.0 20 38.44295537913253 40.0 38.0 41.0 34.0 41.0 21 38.33583095060592 40.0 37.0 41.0 34.0 41.0 22 38.35443267547854 40.0 37.0 41.0 34.0 41.0 23 38.289521643564306 40.0 37.0 41.0 34.0 41.0 24 38.23412493650296 40.0 37.0 41.0 34.0 41.0 25 38.124585814700175 40.0 37.0 41.0 34.0 41.0 26 38.10416805430612 40.0 37.0 41.0 34.0 41.0 27 38.09510633397559 40.0 37.0 41.0 34.0 41.0 28 38.01430358689084 40.0 37.0 41.0 34.0 41.0 29 37.97843949343603 40.0 37.0 41.0 34.0 41.0 30 37.731113591697756 40.0 36.0 41.0 33.0 41.0 31 37.75654771888123 40.0 36.0 41.0 33.0 41.0 32 37.68782245062879 40.0 36.0 41.0 33.0 41.0 33 37.621185262105364 40.0 36.0 41.0 33.0 41.0 34 37.52671771039891 40.0 36.0 41.0 33.0 41.0 35 37.41774354175879 40.0 36.0 41.0 33.0 41.0 36 37.29382860893926 40.0 36.0 41.0 32.0 41.0 37 37.2199874651017 40.0 36.0 41.0 32.0 41.0 38 37.09393710562618 39.0 35.0 41.0 32.0 41.0 39 37.01619906895077 39.0 35.0 41.0 31.0 41.0 40 37.00602108462982 39.0 35.0 41.0 31.0 41.0 41 36.997544379719145 39.0 35.0 41.0 32.0 41.0 42 36.979932125371455 39.0 35.0 41.0 32.0 41.0 43 36.8967692543501 39.0 35.0 41.0 31.0 41.0 44 36.74577829921813 39.0 35.0 41.0 31.0 41.0 45 36.68328035559949 39.0 35.0 41.0 31.0 41.0 46 36.61343962584693 39.0 35.0 40.0 31.0 41.0 47 36.53448019895339 39.0 35.0 40.0 31.0 41.0 48 36.45835918020802 39.0 35.0 40.0 31.0 41.0 49 36.36195281196609 39.0 35.0 40.0 31.0 41.0 50 36.19438160499663 38.0 35.0 40.0 31.0 41.0 51 35.140170978580734 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 14.0 10 15.0 11 11.0 12 7.0 13 9.0 14 16.0 15 26.0 16 57.0 17 86.0 18 156.0 19 330.0 20 626.0 21 1009.0 22 1651.0 23 2686.0 24 4078.0 25 5935.0 26 7918.0 27 9423.0 28 11034.0 29 13391.0 30 17453.0 31 23312.0 32 32301.0 33 50988.0 34 76995.0 35 100370.0 36 124517.0 37 204809.0 38 278589.0 39 278163.0 40 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.129261123117256 4.426295680756524 56.19542564486113 14.249017551265087 2 19.782910327327762 4.51593384591063 52.656202728306475 23.044953098455125 3 19.752014451245103 4.146627815436863 54.053418568501776 22.047939164816256 4 18.469394224156403 4.456950809752825 50.94224397149234 26.131410994598436 5 17.393575744249556 9.384642422365083 50.065924577147804 23.155857256237557 6 15.4708090145339 31.921217923460084 39.736510338883626 12.871462723122393 7 83.61186433741186 1.7919608127942632 8.81126311168819 5.78491173810569 8 85.5578230364467 2.18510080481751 8.281459023642167 3.9756171350936222 9 80.7472147568334 4.184344858966345 10.210485177603138 4.857955206597112 10 41.98332264683767 24.229348514309606 17.840803581674653 15.946525257178076 11 28.216521509548432 27.010057610777764 24.684440756555745 20.088980123118063 12 25.362705547856105 22.481524667347713 29.708190456625 22.44757932817118 13 24.466725141458976 23.11645498310357 30.800459987433005 21.616359888004453 14 21.161909637988607 24.411834805769264 31.747398527109326 22.678857029132804 15 19.77641015599609 24.342499644898048 34.19146294781967 21.689627251286193 16 23.44266728511918 23.86413518430393 31.328338098788162 21.364859431788727 17 22.80372451792402 24.288090803381053 28.023843591432936 24.88434108726199 18 23.01566220294819 24.876637180498523 30.6980622267019 21.409638389851388 19 23.660703896331096 26.623177042999835 29.1637810453399 20.55233801532917 20 24.77760987897644 27.103788476399966 29.26393183326499 18.854669811358608 21 24.287368562121976 27.86430852220611 28.5615120843 19.286810831371916 22 24.43109457267793 24.31176426687296 28.635180692725665 22.62196046772344 23 21.94666489048816 26.50416773331001 28.28698015682265 23.262187219379175 24 21.98133247092377 26.839207428492095 29.067883455940475 22.11157664464366 25 21.602878051168386 27.756774823632696 26.529606675435208 24.110740449763707 26 21.47656607985902 27.175691606192338 27.89207468616611 23.455667627782535 27 21.118093668271378 25.68835610667022 29.68772695428454 23.505823270773867 28 20.561887649754716 25.059604966130898 29.71083867457494 24.66766870953944 29 20.717891761714956 24.913952978884073 30.199796006968825 24.168359252432147 30 21.913201045484346 25.65770097767392 29.41110855205875 23.017989424782986 31 22.675326071866213 26.05862512548942 28.316832795531095 22.949216007113275 32 22.894084924337204 27.145517971368747 27.25722461943904 22.703172484855003 33 22.333224462150948 25.896201091226295 26.788650540356834 24.981923906265923 34 21.560185567854166 25.355242388178993 29.377403959968575 23.707168083998265 35 23.009723774818013 24.9855351125613 28.24292344001907 23.76181767260162 36 23.4489267093645 26.10878076848075 27.170314921263667 23.271977600891088 37 22.198486342819034 26.35490453976781 28.22583039688762 23.220778720525534 38 21.728949275391393 27.10443046863025 28.34997564441976 22.81664461155859 39 21.904052656202726 25.9169855896819 29.06916744040105 23.109794313714318 40 22.028518899850013 24.596648319063718 30.99458238806665 22.38025039301962 41 20.984880280486408 24.534936815927185 29.86892926128362 24.611253642302795 42 22.703734228056504 23.37614084025548 28.769597815942433 25.15052711574558 43 22.642825215207832 23.795442015663003 28.95705954718683 24.60467322194233 44 22.598206755202746 23.75547799932751 28.186829368897566 25.45948587657218 45 22.34638530287187 23.60091836988543 27.32984999049049 26.72284633675221 46 22.62196046772344 23.647623304638955 29.23135072757782 24.499065500059785 47 21.07122823546028 23.989965661440582 31.485626195208972 23.453179907890163 48 21.198663693172655 23.766151120156067 30.843874712006297 24.19131047466498 49 23.214519296280216 22.7662482214809 30.632097525039704 23.38713495719918 50 21.01072046775554 23.21508103948172 30.12339893156443 25.650799561198312 51 20.339437341959567 23.02473034320102 28.596340162793183 28.03949215204623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 157.0 1 651.0 2 1145.0 3 3342.0 4 5539.0 5 3831.0 6 2123.0 7 2062.0 8 2001.0 9 2139.5 10 2278.0 11 2385.0 12 2492.0 13 2530.0 14 2568.0 15 2592.0 16 2616.0 17 2637.0 18 2658.0 19 2634.5 20 2611.0 21 2894.0 22 3177.0 23 3815.5 24 4454.0 25 5363.5 26 7767.0 27 9261.0 28 10758.0 29 12255.0 30 17911.5 31 23568.0 32 23617.0 33 23666.0 34 27595.5 35 31525.0 36 36009.5 37 40494.0 38 46411.5 39 52329.0 40 57331.5 41 62334.0 42 68374.0 43 74414.0 44 82021.0 45 89628.0 46 102451.5 47 115275.0 48 129329.0 49 143383.0 50 136177.5 51 128972.0 52 111426.0 53 93880.0 54 83226.5 55 72573.0 56 64828.5 57 57084.0 58 51634.5 59 46185.0 60 41660.0 61 37135.0 62 34074.0 63 31013.0 64 27501.5 65 23990.0 66 19858.0 67 15726.0 68 12173.5 69 8621.0 70 7224.5 71 5828.0 72 4477.0 73 3126.0 74 2610.0 75 1508.5 76 923.0 77 644.5 78 366.0 79 265.0 80 164.0 81 141.0 82 118.0 83 78.5 84 39.0 85 25.5 86 12.0 87 17.5 88 23.0 89 22.5 90 22.0 91 11.5 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1246121.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.82376912476475 #Duplication Level Percentage of deduplicated Percentage of total 1 72.62817751684759 26.01815063315978 2 11.311308989819814 8.10427443500362 3 4.096544693544104 4.402610139324124 4 2.050261485643281 2.9379237642832834 5 1.4083511931641084 2.5226223995248995 6 0.9791598651426179 2.1046318167062963 7 0.8060467762270419 2.0212943530722924 8 0.644185309251418 1.846171663375039 9 0.5594559900838835 1.8037640001808546 >10 5.336535833659328 39.176802697644106 >50 0.12673050816078937 2.9576532535047413 >100 0.04673191953701669 2.7716989179475537 >500 0.0039524507723491045 0.9973051903323548 >1k 0.002324971042558297 1.884502024090518 >5k 2.324971042558297E-4 0.45059471185051275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA 5410 0.43414724573295854 No Hit GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT 3946 0.3166626675900655 No Hit CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT 2913 0.23376542085399413 TruSeq Adapter, Index 20 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC 2572 0.20640050203792407 TruSeq Adapter, Index 22 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2398 0.19243717102913763 No Hit TCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC 2251 0.18064056379757665 TruSeq Adapter, Index 22 (95% over 21bp) GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGCG 1971 0.1581708357374605 No Hit GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC 1969 0.15801033767988823 TruSeq Adapter, Index 22 (95% over 21bp) GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATG 1960 0.15728809642081307 No Hit GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTA 1625 0.13040467177745982 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.6049805757225823E-4 0.0 0.0 0.248611491179428 0.0 2 1.6049805757225823E-4 0.0 0.0 0.8479914871830264 0.0 3 1.6049805757225823E-4 0.0 0.0 1.2338288175867351 0.0 4 1.6049805757225823E-4 0.0 0.0 1.6855506006238559 0.0 5 1.6049805757225823E-4 0.0 0.0 2.466935393914395 0.0 6 1.6049805757225823E-4 0.0 0.0 3.0262711245537153 0.0 7 1.6049805757225823E-4 8.024902878612912E-5 0.0 3.639534202537314 0.0 8 1.6049805757225823E-4 8.024902878612912E-5 0.0 4.767835547270289 0.0 9 1.6049805757225823E-4 8.024902878612912E-5 0.0 5.241064070022093 0.0 10 1.6049805757225823E-4 8.024902878612912E-5 0.0 6.2265221435157585 0.0 11 1.6049805757225823E-4 8.024902878612912E-5 0.0 8.239167785471876 0.0 12 1.6049805757225823E-4 8.024902878612912E-5 0.0 9.915569996814114 0.0 13 2.4074708635838735E-4 8.024902878612912E-5 0.0 10.637650757831704 0.0 14 2.4074708635838735E-4 8.024902878612912E-5 0.0 10.828803944400264 0.0 15 2.4074708635838735E-4 8.024902878612912E-5 0.0 11.230129337359694 0.0 16 2.4074708635838735E-4 8.024902878612912E-5 0.0 11.891060338442253 0.0 17 2.4074708635838735E-4 8.024902878612912E-5 0.0 12.883419828411526 0.0 18 2.4074708635838735E-4 8.024902878612912E-5 0.0 13.945916969539876 0.0 19 2.4074708635838735E-4 8.024902878612912E-5 0.0 14.716548392973074 0.0 20 3.2099611514451647E-4 8.024902878612912E-5 0.0 15.587410853360147 0.0 21 3.2099611514451647E-4 8.024902878612912E-5 0.0 16.84908608393567 0.0 22 3.2099611514451647E-4 8.024902878612912E-5 0.0 18.232659589237322 0.0 23 3.2099611514451647E-4 8.024902878612912E-5 0.0 19.57089239327481 0.0 24 3.2099611514451647E-4 8.024902878612912E-5 0.0 20.559640676948707 0.0 25 3.2099611514451647E-4 8.024902878612912E-5 0.0 21.57422914789174 0.0 26 3.2099611514451647E-4 8.024902878612912E-5 0.0 22.302970578298577 0.0 27 3.2099611514451647E-4 8.024902878612912E-5 0.0 23.07247851532877 0.0 28 3.2099611514451647E-4 8.024902878612912E-5 0.0 23.846480397970982 0.0 29 3.2099611514451647E-4 8.024902878612912E-5 0.0 24.706027745299213 0.0 30 3.2099611514451647E-4 8.024902878612912E-5 0.0 25.623274144324668 0.0 31 3.2099611514451647E-4 8.024902878612912E-5 0.0 26.46492595823359 0.0 32 3.2099611514451647E-4 8.024902878612912E-5 0.0 27.2846697872839 0.0 33 3.2099611514451647E-4 8.024902878612912E-5 0.0 28.066375576689584 0.0 34 3.2099611514451647E-4 8.024902878612912E-5 0.0 28.941651733659892 0.0 35 3.2099611514451647E-4 8.024902878612912E-5 0.0 29.775519391776562 0.0 36 3.2099611514451647E-4 8.024902878612912E-5 0.0 30.551447251109643 0.0 37 3.2099611514451647E-4 8.024902878612912E-5 0.0 31.325368884723073 0.0 38 4.0124514393064556E-4 8.024902878612912E-5 0.0 32.131630876937315 0.0 39 4.0124514393064556E-4 8.024902878612912E-5 0.0 33.15761470996797 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGGT 20 7.0335146E-4 45.000004 28 ATACTCG 35 1.2120472E-7 45.000004 44 TATTCGC 20 7.0335146E-4 45.000004 25 TTAGTCG 30 2.1656488E-6 45.000004 40 CAATCGT 30 2.1656488E-6 45.000004 38 CGATAAC 20 7.0335146E-4 45.000004 16 CGAGGGT 110 0.0 45.0 4 GTCCGTA 25 3.890995E-5 45.0 9 CGTAACA 25 3.890995E-5 45.0 14 TCGTTAG 165 0.0 43.636364 1 CTTGACC 2050 0.0 43.02439 7 AACTTGA 2065 0.0 42.27603 5 CCTGCAT 1660 0.0 42.153614 13 TAGGGCG 525 0.0 42.0 5 CTCAACT 2150 0.0 41.96512 2 GCATTAG 1590 0.0 41.603775 16 CGTTTAT 595 0.0 41.596638 1 TGCATTA 1585 0.0 41.451103 15 TTTAGGG 4810 0.0 41.21102 3 TTGACCC 1885 0.0 41.06101 8 >>END_MODULE