FastQCFastQC Report
Sat 14 Jan 2017
SRR2934224.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934224.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867945
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86570.4634504763255877No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT36680.1963655246808659No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC29400.15739221443886195No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG25240.13512175144343117No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC25140.13458640377527176No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC24160.1293399966273097No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC19920.10664125549735136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG2450.044.0816351
CGTTTAT16400.044.0396351
TTAGGGT22350.041.9798664
CGTTTTT43150.041.8192331
TTAGGGA42350.041.6528934
TAGGGCG6800.041.0294155
TTTAGGG73550.041.0231133
CTTAGGG34300.040.8673483
TAGGGTG11150.040.762335
TGTAGGG25300.040.4644243
CGTTAGG5400.040.4166682
TCTTAGG21350.040.1522252
AGGGATT18050.040.1385046
GTTAGGG25500.039.882353
CTTTAGG20250.039.777782
TAGGGAA18650.039.5710455
TAGGGAT27200.039.457725
TTAGGGG58000.039.3754
TAGGGTA10000.039.3755
AGGGCGA10150.039.236456
Produced by FastQC (version 0.11.5)