##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934224.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1867945 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39776920626678 33.0 31.0 34.0 31.0 34.0 2 32.5769977167422 34.0 31.0 34.0 31.0 34.0 3 32.60917050555557 34.0 31.0 34.0 31.0 34.0 4 36.07433088233326 37.0 35.0 37.0 35.0 37.0 5 35.21246824719143 37.0 35.0 37.0 35.0 37.0 6 26.282607357283002 37.0 0.0 37.0 0.0 37.0 7 31.239708877938053 37.0 19.0 37.0 17.0 37.0 8 34.88497894745295 37.0 32.0 37.0 32.0 37.0 9 37.407287152458984 39.0 37.0 39.0 35.0 39.0 10 37.34354169956824 39.0 37.0 39.0 34.0 39.0 11 37.433986011365434 39.0 37.0 39.0 35.0 39.0 12 37.1657629105782 39.0 37.0 39.0 34.0 39.0 13 37.1665868106395 39.0 37.0 39.0 34.0 39.0 14 38.03449726838852 40.0 38.0 41.0 33.0 41.0 15 38.24293541833405 40.0 38.0 41.0 33.0 41.0 16 38.41995883176432 40.0 38.0 41.0 33.0 41.0 17 38.46475779533123 40.0 38.0 41.0 34.0 41.0 18 38.43395603189602 40.0 38.0 41.0 34.0 41.0 19 38.4339142747779 40.0 37.0 41.0 34.0 41.0 20 38.31228167852908 40.0 37.0 41.0 34.0 41.0 21 38.21612038898362 40.0 37.0 41.0 34.0 41.0 22 38.26204251195833 40.0 37.0 41.0 34.0 41.0 23 38.22191017401476 40.0 36.0 41.0 34.0 41.0 24 38.175037273581395 40.0 36.0 41.0 34.0 41.0 25 38.05533085824261 40.0 36.0 41.0 34.0 41.0 26 38.03679283918959 40.0 36.0 41.0 34.0 41.0 27 38.02068583389768 40.0 36.0 41.0 34.0 41.0 28 37.95548637674021 40.0 36.0 41.0 34.0 41.0 29 37.93469454400424 40.0 36.0 41.0 34.0 41.0 30 37.684855282141605 40.0 36.0 41.0 33.0 41.0 31 37.643000195401896 40.0 36.0 41.0 33.0 41.0 32 37.58420992052764 40.0 36.0 41.0 33.0 41.0 33 37.46272668627824 40.0 35.0 41.0 33.0 41.0 34 37.29623516752367 40.0 35.0 41.0 32.0 41.0 35 37.26143756909331 40.0 35.0 41.0 32.0 41.0 36 37.20451833431927 40.0 35.0 41.0 32.0 41.0 37 37.11731501730511 40.0 35.0 41.0 32.0 41.0 38 37.05084946291245 40.0 35.0 41.0 32.0 41.0 39 36.98293739912042 40.0 35.0 41.0 32.0 41.0 40 36.89244437068543 39.0 35.0 41.0 31.0 41.0 41 36.77002695475509 39.0 35.0 41.0 31.0 41.0 42 36.77123844652814 39.0 35.0 41.0 31.0 41.0 43 36.74911627483679 39.0 35.0 41.0 31.0 41.0 44 36.67397808821994 39.0 35.0 41.0 31.0 41.0 45 36.661276429445195 39.0 35.0 41.0 31.0 41.0 46 36.63170543029907 39.0 35.0 41.0 31.0 41.0 47 36.5638731333096 39.0 35.0 41.0 31.0 41.0 48 36.50359030913651 39.0 35.0 41.0 31.0 41.0 49 36.48507477468555 39.0 35.0 41.0 31.0 41.0 50 36.383561614501495 39.0 35.0 41.0 31.0 41.0 51 35.388870657326635 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 12.0 10 17.0 11 14.0 12 12.0 13 16.0 14 21.0 15 42.0 16 69.0 17 198.0 18 326.0 19 555.0 20 1038.0 21 1777.0 22 2855.0 23 4480.0 24 7445.0 25 11662.0 26 15314.0 27 17270.0 28 18506.0 29 21782.0 30 26452.0 31 35676.0 32 50738.0 33 82913.0 34 121514.0 35 131862.0 36 159630.0 37 274634.0 38 410181.0 39 470642.0 40 289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.649673839433174 4.140111191710677 57.767814362842586 16.44240060601356 2 21.446884142734397 3.7186855073356013 54.36262844998113 20.471801899948876 3 20.440109317993837 3.8774696257116776 54.40240478172538 21.28001627456911 4 18.43726662187591 4.178174410916809 53.3119551164515 24.072603850755776 5 17.94897601374773 7.509803554173169 51.713942326995706 22.827278105083394 6 15.80865603644647 30.290934690261224 41.00067186132354 12.899737411968767 7 85.1135338567249 1.368455709349044 9.693540227362154 3.824470206563898 8 87.7712138205354 1.469315210030274 7.502308686818937 3.2571622826153876 9 82.72433074849634 3.6572275950309026 9.725179274550374 3.89326238192238 10 38.653065266910964 26.06613149744773 22.58503328524127 12.695769950400038 11 29.993281386764597 22.09192454809965 28.518559165285918 19.396234899849834 12 27.72651229024409 20.24443974528158 31.100969246953202 20.928078717521124 13 27.130027918380893 19.643030174871313 33.31318641608827 19.913755490659522 14 21.20790494366804 21.986086314104536 35.45869926577067 21.347309476456747 15 19.656842144709827 23.64368329902647 37.15982001611396 19.539654540149737 16 25.177347298769504 23.2108547093196 31.87604560091437 19.735752390996524 17 25.455246273311044 23.046984788096008 29.691612975756783 21.806155962836165 18 24.614215086632637 22.730058968545645 32.207051064137325 20.44867488068439 19 24.626099804865774 24.831298566071272 29.942048614921745 20.60055301414121 20 23.31417680927436 28.96744818503757 29.175751962718387 18.54262304296968 21 23.717882485833364 25.629662543597377 32.66964498419386 17.982809986375404 22 22.02227581647211 21.924039519364865 34.0421158010541 22.011568863108923 23 21.339386330967987 23.802628021702997 33.087002026290925 21.77098362103809 24 21.380393962348997 25.9347036449146 30.57525783682068 22.109644555915725 25 23.04500400172382 25.435973757257308 28.467486997743507 23.051535243275364 26 20.11231594077984 26.092684741788435 28.449874059461067 25.345125257970658 27 19.716801083543682 24.7051171206861 33.07062038764524 22.50746140812497 28 18.440906986019396 24.930230815147127 31.36403909108673 25.264823107746746 29 21.274502193587068 25.28388148473322 30.119195158315687 23.32242116336402 30 24.290650955997098 22.497182732896313 31.059265663603586 22.152900647503003 31 21.81504273412761 24.381927733418273 31.767691232878914 22.0353382995752 32 24.810045263645343 24.514105072686828 28.05537636279441 22.62047330087342 33 25.342769728230756 23.38714469644449 29.017128448642758 22.252957126681995 34 20.635939495006543 23.491002144067412 32.35052423920404 23.522534121722 35 20.65382010712307 25.6045547379607 28.992877199275142 24.748747955641093 36 23.45438436356531 24.78702531391449 28.746028389486845 23.01256193303336 37 20.827701029741238 26.84570477182144 30.726440018308892 21.60015418012843 38 23.83614078572977 24.641143074341056 29.47779511709392 22.044921022835254 39 22.98156530304693 23.76408298959552 28.65571523786835 24.5986364694892 40 23.79952300522767 22.475233478501778 30.63575212332269 23.089491392947863 41 20.255414372478846 23.50706257411219 29.453865076327197 26.783657977081766 42 19.703845669974225 24.468654055660096 30.760220456169744 25.06727981819593 43 19.867019639229206 23.871473731828292 31.413398146091026 24.848108482851476 44 22.562548683178573 22.8293124262224 29.63218938459109 24.97594950600794 45 21.77944211419501 22.311631231112266 28.080055890296556 27.828870764396168 46 21.158010540995587 22.575932374882555 30.617710906905714 25.648346177216137 47 18.838830907762276 23.682977817869368 33.09877967499043 24.379411599377924 48 19.792017430920076 25.356046350401108 30.99475626959038 23.857179949088437 49 23.344477487292185 22.352799466793723 30.931531709980753 23.37119133593334 50 21.424346005904884 22.85897068703843 29.441337940892264 26.27534536616442 51 19.84062699918895 23.17712780622556 28.576323178680312 28.405922015905176 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 249.0 1 782.0 2 1315.0 3 9867.5 4 18420.0 5 11768.0 6 5116.0 7 4946.0 8 4776.0 9 4915.5 10 5055.0 11 5137.5 12 5220.0 13 5159.5 14 5099.0 15 4995.0 16 4891.0 17 4746.0 18 4601.0 19 4732.5 20 4864.0 21 5608.5 22 6353.0 23 6933.0 24 7513.0 25 9041.0 26 13166.5 27 15764.0 28 18947.0 29 22130.0 30 26203.0 31 30276.0 32 33675.5 33 37075.0 34 43154.0 35 49233.0 36 52897.5 37 56562.0 38 62935.5 39 69309.0 40 75892.5 41 82476.0 42 91002.0 43 99528.0 44 113236.0 45 126944.0 46 147110.0 47 167276.0 48 189085.0 49 210894.0 50 206141.5 51 201389.0 52 178508.0 53 155627.0 54 137946.0 55 120265.0 56 109656.5 57 99048.0 58 89152.0 59 79256.0 60 67623.5 61 55991.0 62 47646.5 63 39302.0 64 32217.5 65 25133.0 66 20772.0 67 16411.0 68 13089.5 69 9768.0 70 8023.5 71 6279.0 72 4845.5 73 3412.0 74 2788.5 75 1655.5 76 1146.0 77 851.5 78 557.0 79 408.0 80 259.0 81 195.0 82 131.0 83 142.0 84 153.0 85 128.5 86 104.0 87 66.5 88 29.0 89 15.5 90 2.0 91 3.5 92 5.0 93 4.5 94 4.0 95 2.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1867945.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.935860294149556 #Duplication Level Percentage of deduplicated Percentage of total 1 72.26726442762518 25.247190538838773 2 11.390729643438911 7.9588987894321965 3 4.192174923787967 4.393717123982804 4 2.1481359214117113 3.001879057731351 5 1.228585601926023 2.1460847474145592 6 0.8943965769227317 1.8747908315362887 7 0.681559805293813 1.66676146978967 8 0.5489458751917001 1.5342317123797515 9 0.4873709607251196 1.5324051415786406 >10 5.9850828775795515 42.07425668031598 >50 0.11648228774391754 2.7784323292818 >100 0.054670978709715705 3.3688467679245333 >500 0.0031846982148596285 0.7967464533129194 >1k 0.0012384937502231887 1.0906587407840547 >5k 1.7692767860331268E-4 0.5350996156966598 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8657 0.4634504763255877 No Hit CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT 3668 0.1963655246808659 No Hit CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 2940 0.15739221443886195 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 2524 0.13512175144343117 No Hit TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 2514 0.13458640377527176 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC 2416 0.1293399966273097 No Hit GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC 1992 0.10664125549735136 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.20728661711131752 0.0 2 0.0 0.0 0.0 0.6407576240199792 0.0 3 0.0 0.0 0.0 0.9411947353910313 0.0 4 0.0 0.0 0.0 1.3028220852327022 0.0 5 0.0 0.0 0.0 1.9814287893915505 0.0 6 0.0 0.0 0.0 2.693708861877625 0.0 7 0.0 0.0 0.0 3.5190008271121473 0.0 8 0.0 0.0 0.0 4.757795331232986 0.0 9 0.0 0.0 0.0 5.47917631407777 0.0 10 0.0 0.0 0.0 6.607956872391853 0.0 11 0.0 0.0 0.0 8.463043612097787 0.0 12 0.0 0.0 0.0 10.029096145764463 0.0 13 5.353476681593944E-5 0.0 0.0 10.572259889878985 0.0 14 5.353476681593944E-5 0.0 0.0 10.857814336075206 0.0 15 5.353476681593944E-5 0.0 0.0 11.120348832540573 0.0 16 1.0706953363187888E-4 0.0 0.0 11.647987494278471 0.0 17 1.6060430044781833E-4 0.0 0.0 12.418406323526657 0.0 18 1.6060430044781833E-4 0.0 0.0 13.258688023469642 0.0 19 1.6060430044781833E-4 0.0 0.0 13.885794281951556 0.0 20 2.1413906726375776E-4 0.0 0.0 14.586778518639468 0.0 21 2.1413906726375776E-4 0.0 0.0 15.563573873963099 0.0 22 2.1413906726375776E-4 0.0 0.0 16.663017380062048 0.0 23 2.1413906726375776E-4 0.0 0.0 17.68092743630032 0.0 24 2.1413906726375776E-4 0.0 0.0 18.385016689463555 0.0 25 2.1413906726375776E-4 0.0 0.0 19.064801158492354 0.0 26 2.1413906726375776E-4 0.0 0.0 19.64972202072331 0.0 27 2.1413906726375776E-4 0.0 0.0 20.319388418823895 0.0 28 2.1413906726375776E-4 0.0 0.0 20.95516730952999 0.0 29 2.1413906726375776E-4 0.0 0.0 21.67237258056313 0.0 30 2.1413906726375776E-4 0.0 0.0 22.609498673676153 0.0 31 2.1413906726375776E-4 0.0 0.0 23.33912401061059 0.0 32 2.1413906726375776E-4 0.0 0.0 24.004828835966798 0.0 33 2.1413906726375776E-4 0.0 0.0 24.6574176434531 5.353476681593944E-5 34 2.1413906726375776E-4 0.0 0.0 25.37173203707818 5.353476681593944E-5 35 2.1413906726375776E-4 0.0 0.0 26.166348580927167 5.353476681593944E-5 36 2.1413906726375776E-4 0.0 0.0 26.879967022583642 5.353476681593944E-5 37 2.1413906726375776E-4 0.0 0.0 27.587375431289466 5.353476681593944E-5 38 2.1413906726375776E-4 0.0 0.0 28.439970127600116 5.353476681593944E-5 39 2.1413906726375776E-4 0.0 0.0 30.15677656462048 5.353476681593944E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTAG 245 0.0 44.081635 1 CGTTTAT 1640 0.0 44.039635 1 TTAGGGT 2235 0.0 41.979866 4 CGTTTTT 4315 0.0 41.819233 1 TTAGGGA 4235 0.0 41.652893 4 TAGGGCG 680 0.0 41.029415 5 TTTAGGG 7355 0.0 41.023113 3 CTTAGGG 3430 0.0 40.867348 3 TAGGGTG 1115 0.0 40.76233 5 TGTAGGG 2530 0.0 40.464424 3 CGTTAGG 540 0.0 40.416668 2 TCTTAGG 2135 0.0 40.152225 2 AGGGATT 1805 0.0 40.138504 6 GTTAGGG 2550 0.0 39.88235 3 CTTTAGG 2025 0.0 39.77778 2 TAGGGAA 1865 0.0 39.571045 5 TAGGGAT 2720 0.0 39.45772 5 TTAGGGG 5800 0.0 39.375 4 TAGGGTA 1000 0.0 39.375 5 AGGGCGA 1015 0.0 39.23645 6 >>END_MODULE