##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1867945
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39776920626678	33.0	31.0	34.0	31.0	34.0
2	32.5769977167422	34.0	31.0	34.0	31.0	34.0
3	32.60917050555557	34.0	31.0	34.0	31.0	34.0
4	36.07433088233326	37.0	35.0	37.0	35.0	37.0
5	35.21246824719143	37.0	35.0	37.0	35.0	37.0
6	26.282607357283002	37.0	0.0	37.0	0.0	37.0
7	31.239708877938053	37.0	19.0	37.0	17.0	37.0
8	34.88497894745295	37.0	32.0	37.0	32.0	37.0
9	37.407287152458984	39.0	37.0	39.0	35.0	39.0
10	37.34354169956824	39.0	37.0	39.0	34.0	39.0
11	37.433986011365434	39.0	37.0	39.0	35.0	39.0
12	37.1657629105782	39.0	37.0	39.0	34.0	39.0
13	37.1665868106395	39.0	37.0	39.0	34.0	39.0
14	38.03449726838852	40.0	38.0	41.0	33.0	41.0
15	38.24293541833405	40.0	38.0	41.0	33.0	41.0
16	38.41995883176432	40.0	38.0	41.0	33.0	41.0
17	38.46475779533123	40.0	38.0	41.0	34.0	41.0
18	38.43395603189602	40.0	38.0	41.0	34.0	41.0
19	38.4339142747779	40.0	37.0	41.0	34.0	41.0
20	38.31228167852908	40.0	37.0	41.0	34.0	41.0
21	38.21612038898362	40.0	37.0	41.0	34.0	41.0
22	38.26204251195833	40.0	37.0	41.0	34.0	41.0
23	38.22191017401476	40.0	36.0	41.0	34.0	41.0
24	38.175037273581395	40.0	36.0	41.0	34.0	41.0
25	38.05533085824261	40.0	36.0	41.0	34.0	41.0
26	38.03679283918959	40.0	36.0	41.0	34.0	41.0
27	38.02068583389768	40.0	36.0	41.0	34.0	41.0
28	37.95548637674021	40.0	36.0	41.0	34.0	41.0
29	37.93469454400424	40.0	36.0	41.0	34.0	41.0
30	37.684855282141605	40.0	36.0	41.0	33.0	41.0
31	37.643000195401896	40.0	36.0	41.0	33.0	41.0
32	37.58420992052764	40.0	36.0	41.0	33.0	41.0
33	37.46272668627824	40.0	35.0	41.0	33.0	41.0
34	37.29623516752367	40.0	35.0	41.0	32.0	41.0
35	37.26143756909331	40.0	35.0	41.0	32.0	41.0
36	37.20451833431927	40.0	35.0	41.0	32.0	41.0
37	37.11731501730511	40.0	35.0	41.0	32.0	41.0
38	37.05084946291245	40.0	35.0	41.0	32.0	41.0
39	36.98293739912042	40.0	35.0	41.0	32.0	41.0
40	36.89244437068543	39.0	35.0	41.0	31.0	41.0
41	36.77002695475509	39.0	35.0	41.0	31.0	41.0
42	36.77123844652814	39.0	35.0	41.0	31.0	41.0
43	36.74911627483679	39.0	35.0	41.0	31.0	41.0
44	36.67397808821994	39.0	35.0	41.0	31.0	41.0
45	36.661276429445195	39.0	35.0	41.0	31.0	41.0
46	36.63170543029907	39.0	35.0	41.0	31.0	41.0
47	36.5638731333096	39.0	35.0	41.0	31.0	41.0
48	36.50359030913651	39.0	35.0	41.0	31.0	41.0
49	36.48507477468555	39.0	35.0	41.0	31.0	41.0
50	36.383561614501495	39.0	35.0	41.0	31.0	41.0
51	35.388870657326635	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	17.0
11	14.0
12	12.0
13	16.0
14	21.0
15	42.0
16	69.0
17	198.0
18	326.0
19	555.0
20	1038.0
21	1777.0
22	2855.0
23	4480.0
24	7445.0
25	11662.0
26	15314.0
27	17270.0
28	18506.0
29	21782.0
30	26452.0
31	35676.0
32	50738.0
33	82913.0
34	121514.0
35	131862.0
36	159630.0
37	274634.0
38	410181.0
39	470642.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.649673839433174	4.140111191710677	57.767814362842586	16.44240060601356
2	21.446884142734397	3.7186855073356013	54.36262844998113	20.471801899948876
3	20.440109317993837	3.8774696257116776	54.40240478172538	21.28001627456911
4	18.43726662187591	4.178174410916809	53.3119551164515	24.072603850755776
5	17.94897601374773	7.509803554173169	51.713942326995706	22.827278105083394
6	15.80865603644647	30.290934690261224	41.00067186132354	12.899737411968767
7	85.1135338567249	1.368455709349044	9.693540227362154	3.824470206563898
8	87.7712138205354	1.469315210030274	7.502308686818937	3.2571622826153876
9	82.72433074849634	3.6572275950309026	9.725179274550374	3.89326238192238
10	38.653065266910964	26.06613149744773	22.58503328524127	12.695769950400038
11	29.993281386764597	22.09192454809965	28.518559165285918	19.396234899849834
12	27.72651229024409	20.24443974528158	31.100969246953202	20.928078717521124
13	27.130027918380893	19.643030174871313	33.31318641608827	19.913755490659522
14	21.20790494366804	21.986086314104536	35.45869926577067	21.347309476456747
15	19.656842144709827	23.64368329902647	37.15982001611396	19.539654540149737
16	25.177347298769504	23.2108547093196	31.87604560091437	19.735752390996524
17	25.455246273311044	23.046984788096008	29.691612975756783	21.806155962836165
18	24.614215086632637	22.730058968545645	32.207051064137325	20.44867488068439
19	24.626099804865774	24.831298566071272	29.942048614921745	20.60055301414121
20	23.31417680927436	28.96744818503757	29.175751962718387	18.54262304296968
21	23.717882485833364	25.629662543597377	32.66964498419386	17.982809986375404
22	22.02227581647211	21.924039519364865	34.0421158010541	22.011568863108923
23	21.339386330967987	23.802628021702997	33.087002026290925	21.77098362103809
24	21.380393962348997	25.9347036449146	30.57525783682068	22.109644555915725
25	23.04500400172382	25.435973757257308	28.467486997743507	23.051535243275364
26	20.11231594077984	26.092684741788435	28.449874059461067	25.345125257970658
27	19.716801083543682	24.7051171206861	33.07062038764524	22.50746140812497
28	18.440906986019396	24.930230815147127	31.36403909108673	25.264823107746746
29	21.274502193587068	25.28388148473322	30.119195158315687	23.32242116336402
30	24.290650955997098	22.497182732896313	31.059265663603586	22.152900647503003
31	21.81504273412761	24.381927733418273	31.767691232878914	22.0353382995752
32	24.810045263645343	24.514105072686828	28.05537636279441	22.62047330087342
33	25.342769728230756	23.38714469644449	29.017128448642758	22.252957126681995
34	20.635939495006543	23.491002144067412	32.35052423920404	23.522534121722
35	20.65382010712307	25.6045547379607	28.992877199275142	24.748747955641093
36	23.45438436356531	24.78702531391449	28.746028389486845	23.01256193303336
37	20.827701029741238	26.84570477182144	30.726440018308892	21.60015418012843
38	23.83614078572977	24.641143074341056	29.47779511709392	22.044921022835254
39	22.98156530304693	23.76408298959552	28.65571523786835	24.5986364694892
40	23.79952300522767	22.475233478501778	30.63575212332269	23.089491392947863
41	20.255414372478846	23.50706257411219	29.453865076327197	26.783657977081766
42	19.703845669974225	24.468654055660096	30.760220456169744	25.06727981819593
43	19.867019639229206	23.871473731828292	31.413398146091026	24.848108482851476
44	22.562548683178573	22.8293124262224	29.63218938459109	24.97594950600794
45	21.77944211419501	22.311631231112266	28.080055890296556	27.828870764396168
46	21.158010540995587	22.575932374882555	30.617710906905714	25.648346177216137
47	18.838830907762276	23.682977817869368	33.09877967499043	24.379411599377924
48	19.792017430920076	25.356046350401108	30.99475626959038	23.857179949088437
49	23.344477487292185	22.352799466793723	30.931531709980753	23.37119133593334
50	21.424346005904884	22.85897068703843	29.441337940892264	26.27534536616442
51	19.84062699918895	23.17712780622556	28.576323178680312	28.405922015905176
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	782.0
2	1315.0
3	9867.5
4	18420.0
5	11768.0
6	5116.0
7	4946.0
8	4776.0
9	4915.5
10	5055.0
11	5137.5
12	5220.0
13	5159.5
14	5099.0
15	4995.0
16	4891.0
17	4746.0
18	4601.0
19	4732.5
20	4864.0
21	5608.5
22	6353.0
23	6933.0
24	7513.0
25	9041.0
26	13166.5
27	15764.0
28	18947.0
29	22130.0
30	26203.0
31	30276.0
32	33675.5
33	37075.0
34	43154.0
35	49233.0
36	52897.5
37	56562.0
38	62935.5
39	69309.0
40	75892.5
41	82476.0
42	91002.0
43	99528.0
44	113236.0
45	126944.0
46	147110.0
47	167276.0
48	189085.0
49	210894.0
50	206141.5
51	201389.0
52	178508.0
53	155627.0
54	137946.0
55	120265.0
56	109656.5
57	99048.0
58	89152.0
59	79256.0
60	67623.5
61	55991.0
62	47646.5
63	39302.0
64	32217.5
65	25133.0
66	20772.0
67	16411.0
68	13089.5
69	9768.0
70	8023.5
71	6279.0
72	4845.5
73	3412.0
74	2788.5
75	1655.5
76	1146.0
77	851.5
78	557.0
79	408.0
80	259.0
81	195.0
82	131.0
83	142.0
84	153.0
85	128.5
86	104.0
87	66.5
88	29.0
89	15.5
90	2.0
91	3.5
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1867945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.935860294149556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26726442762518	25.247190538838773
2	11.390729643438911	7.9588987894321965
3	4.192174923787967	4.393717123982804
4	2.1481359214117113	3.001879057731351
5	1.228585601926023	2.1460847474145592
6	0.8943965769227317	1.8747908315362887
7	0.681559805293813	1.66676146978967
8	0.5489458751917001	1.5342317123797515
9	0.4873709607251196	1.5324051415786406
>10	5.9850828775795515	42.07425668031598
>50	0.11648228774391754	2.7784323292818
>100	0.054670978709715705	3.3688467679245333
>500	0.0031846982148596285	0.7967464533129194
>1k	0.0012384937502231887	1.0906587407840547
>5k	1.7692767860331268E-4	0.5350996156966598
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8657	0.4634504763255877	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	3668	0.1963655246808659	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2940	0.15739221443886195	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2524	0.13512175144343117	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2514	0.13458640377527176	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2416	0.1293399966273097	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1992	0.10664125549735136	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20728661711131752	0.0
2	0.0	0.0	0.0	0.6407576240199792	0.0
3	0.0	0.0	0.0	0.9411947353910313	0.0
4	0.0	0.0	0.0	1.3028220852327022	0.0
5	0.0	0.0	0.0	1.9814287893915505	0.0
6	0.0	0.0	0.0	2.693708861877625	0.0
7	0.0	0.0	0.0	3.5190008271121473	0.0
8	0.0	0.0	0.0	4.757795331232986	0.0
9	0.0	0.0	0.0	5.47917631407777	0.0
10	0.0	0.0	0.0	6.607956872391853	0.0
11	0.0	0.0	0.0	8.463043612097787	0.0
12	0.0	0.0	0.0	10.029096145764463	0.0
13	5.353476681593944E-5	0.0	0.0	10.572259889878985	0.0
14	5.353476681593944E-5	0.0	0.0	10.857814336075206	0.0
15	5.353476681593944E-5	0.0	0.0	11.120348832540573	0.0
16	1.0706953363187888E-4	0.0	0.0	11.647987494278471	0.0
17	1.6060430044781833E-4	0.0	0.0	12.418406323526657	0.0
18	1.6060430044781833E-4	0.0	0.0	13.258688023469642	0.0
19	1.6060430044781833E-4	0.0	0.0	13.885794281951556	0.0
20	2.1413906726375776E-4	0.0	0.0	14.586778518639468	0.0
21	2.1413906726375776E-4	0.0	0.0	15.563573873963099	0.0
22	2.1413906726375776E-4	0.0	0.0	16.663017380062048	0.0
23	2.1413906726375776E-4	0.0	0.0	17.68092743630032	0.0
24	2.1413906726375776E-4	0.0	0.0	18.385016689463555	0.0
25	2.1413906726375776E-4	0.0	0.0	19.064801158492354	0.0
26	2.1413906726375776E-4	0.0	0.0	19.64972202072331	0.0
27	2.1413906726375776E-4	0.0	0.0	20.319388418823895	0.0
28	2.1413906726375776E-4	0.0	0.0	20.95516730952999	0.0
29	2.1413906726375776E-4	0.0	0.0	21.67237258056313	0.0
30	2.1413906726375776E-4	0.0	0.0	22.609498673676153	0.0
31	2.1413906726375776E-4	0.0	0.0	23.33912401061059	0.0
32	2.1413906726375776E-4	0.0	0.0	24.004828835966798	0.0
33	2.1413906726375776E-4	0.0	0.0	24.6574176434531	5.353476681593944E-5
34	2.1413906726375776E-4	0.0	0.0	25.37173203707818	5.353476681593944E-5
35	2.1413906726375776E-4	0.0	0.0	26.166348580927167	5.353476681593944E-5
36	2.1413906726375776E-4	0.0	0.0	26.879967022583642	5.353476681593944E-5
37	2.1413906726375776E-4	0.0	0.0	27.587375431289466	5.353476681593944E-5
38	2.1413906726375776E-4	0.0	0.0	28.439970127600116	5.353476681593944E-5
39	2.1413906726375776E-4	0.0	0.0	30.15677656462048	5.353476681593944E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	245	0.0	44.081635	1
CGTTTAT	1640	0.0	44.039635	1
TTAGGGT	2235	0.0	41.979866	4
CGTTTTT	4315	0.0	41.819233	1
TTAGGGA	4235	0.0	41.652893	4
TAGGGCG	680	0.0	41.029415	5
TTTAGGG	7355	0.0	41.023113	3
CTTAGGG	3430	0.0	40.867348	3
TAGGGTG	1115	0.0	40.76233	5
TGTAGGG	2530	0.0	40.464424	3
CGTTAGG	540	0.0	40.416668	2
TCTTAGG	2135	0.0	40.152225	2
AGGGATT	1805	0.0	40.138504	6
GTTAGGG	2550	0.0	39.88235	3
CTTTAGG	2025	0.0	39.77778	2
TAGGGAA	1865	0.0	39.571045	5
TAGGGAT	2720	0.0	39.45772	5
TTAGGGG	5800	0.0	39.375	4
TAGGGTA	1000	0.0	39.375	5
AGGGCGA	1015	0.0	39.23645	6
>>END_MODULE