FastQCFastQC Report
Sat 14 Jan 2017
SRR2934223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771948
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79550.448940939576105No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT37900.21388889515945161Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC33670.19001686279732816No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC23740.1339768435642581No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC22680.12799472670755574No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG18430.1040098242160605No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTA207.034321E-445.0000049
GCGATCG302.1661708E-645.0000049
CGTTTAT16150.042.9102171
TAGGGCG7100.042.781695
TTAGGGA32650.041.692194
TTTAGGG61000.041.643443
CGTTTTT40450.041.0506741
TCGAGGG8400.040.9821433
CTAGGGA20350.040.6879584
TGTTAGG15500.040.354842
TTAGGGG57850.040.06054
TGTAGGG21900.039.7602733
GTTAGGG24750.039.7272723
GCTAGGG19650.039.618323
TAGGGAG21300.039.612685
TTTTAGG35750.039.5244752
TAGGGAC13900.039.4964035
TAGGGGA31900.039.42795
TTCGTAG3550.039.2957731
TAGGGAA16050.039.252345
Produced by FastQC (version 0.11.5)