##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934223.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1771948
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40469415581044	33.0	31.0	34.0	31.0	34.0
2	32.5796953409468	34.0	31.0	34.0	31.0	34.0
3	32.60968549867152	34.0	31.0	34.0	31.0	34.0
4	36.082051504897436	37.0	35.0	37.0	35.0	37.0
5	35.19276017129171	37.0	35.0	37.0	35.0	37.0
6	26.041384397284798	37.0	0.0	37.0	0.0	37.0
7	31.090511685444493	37.0	19.0	37.0	17.0	37.0
8	34.79845063173411	37.0	32.0	37.0	32.0	37.0
9	37.33275863625795	39.0	37.0	39.0	35.0	39.0
10	37.453165104167844	39.0	37.0	39.0	35.0	39.0
11	37.508555555806375	39.0	37.0	39.0	35.0	39.0
12	37.220894179738906	39.0	37.0	39.0	34.0	39.0
13	37.226390955039314	39.0	37.0	39.0	34.0	39.0
14	38.3129606512155	40.0	38.0	41.0	33.0	41.0
15	38.46750468975387	40.0	38.0	41.0	34.0	41.0
16	38.57996961536117	40.0	38.0	41.0	34.0	41.0
17	38.56823394365975	40.0	38.0	41.0	34.0	41.0
18	38.53010923571121	40.0	38.0	41.0	34.0	41.0
19	38.52316828710549	40.0	37.0	41.0	34.0	41.0
20	38.44241591739713	40.0	37.0	41.0	34.0	41.0
21	38.32922128640344	40.0	37.0	41.0	34.0	41.0
22	38.3451991819173	40.0	37.0	41.0	34.0	41.0
23	38.29582075771975	40.0	37.0	41.0	34.0	41.0
24	38.26808405212794	40.0	37.0	41.0	34.0	41.0
25	38.15241643659972	40.0	36.0	41.0	34.0	41.0
26	38.11156760807879	40.0	36.0	41.0	34.0	41.0
27	38.07622966362444	40.0	36.0	41.0	34.0	41.0
28	37.983767582344406	40.0	36.0	41.0	34.0	41.0
29	37.96243399919185	40.0	36.0	41.0	34.0	41.0
30	37.708690661351234	40.0	36.0	41.0	33.0	41.0
31	37.682224309065504	40.0	36.0	41.0	33.0	41.0
32	37.57472058999474	40.0	36.0	41.0	33.0	41.0
33	37.45440046773382	40.0	36.0	41.0	33.0	41.0
34	37.302350294703906	40.0	35.0	41.0	33.0	41.0
35	37.23190409650847	40.0	35.0	41.0	32.0	41.0
36	37.144539794621515	40.0	35.0	41.0	32.0	41.0
37	37.060618031680384	40.0	35.0	41.0	32.0	41.0
38	36.97740057834654	39.0	35.0	41.0	32.0	41.0
39	36.93228187283148	39.0	35.0	41.0	32.0	41.0
40	36.8804750478005	39.0	35.0	41.0	32.0	41.0
41	36.75810238223695	39.0	35.0	41.0	31.0	41.0
42	36.6547370464596	39.0	35.0	41.0	31.0	41.0
43	36.57768851004657	39.0	35.0	41.0	31.0	41.0
44	36.49028583231562	39.0	35.0	41.0	31.0	41.0
45	36.46454072015657	39.0	35.0	41.0	31.0	41.0
46	36.423203728326115	39.0	35.0	41.0	31.0	41.0
47	36.320334456767355	39.0	35.0	40.0	31.0	41.0
48	36.26101838202927	39.0	35.0	40.0	31.0	41.0
49	36.2303002119701	39.0	35.0	40.0	31.0	41.0
50	36.15442834665577	38.0	35.0	40.0	31.0	41.0
51	35.181597315496845	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	17.0
10	23.0
11	19.0
12	15.0
13	12.0
14	19.0
15	31.0
16	80.0
17	146.0
18	275.0
19	577.0
20	1033.0
21	1656.0
22	2698.0
23	4269.0
24	6884.0
25	10609.0
26	14190.0
27	16073.0
28	17562.0
29	19866.0
30	24559.0
31	32399.0
32	45436.0
33	74871.0
34	113543.0
35	136291.0
36	164991.0
37	277138.0
38	387896.0
39	418516.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.844408526661052	4.312146857582729	53.946109028030165	16.89733558772605
2	22.374245745360476	4.357069169072682	51.16086928058837	22.107815804978475
3	21.58449345014639	4.2353951696099434	51.72804168068137	22.45206969956229
4	19.00541099400208	4.701830979238668	51.16888305977376	25.123874966985486
5	18.274915516708166	7.85677683543761	49.596658592690076	24.271649055164147
6	16.407817836640803	31.277328680074135	39.14511035312548	13.169743130159576
7	83.04267393851286	2.263441139356234	10.377448999632044	4.316435922498854
8	84.71213602205032	2.341942314334281	8.842810285629149	4.103111377986261
9	79.32004776663875	4.485289636038981	11.066577574511216	5.128085022811053
10	40.72393772277742	24.90338316925779	18.929900877452386	15.442778230512408
11	30.996902843650037	23.73991787569387	26.09382442374155	19.169354856914538
12	27.3746182167874	21.92609489668997	30.424086937088447	20.27519994943418
13	25.7820771264168	21.85995300087813	32.11883192960516	20.239137943099912
14	21.14057523132733	23.518128071478394	34.14304482975798	21.19825186743629
15	19.82129272416572	23.558196967405365	35.16457593563694	21.45593437279198
16	24.2079338671338	24.199976523013092	30.774379383593647	20.81771022625946
17	24.36177585346748	24.017465523818984	29.85934124477693	21.7614173779366
18	24.329325691273105	22.91771541828541	31.497086821960917	21.255872068480564
19	24.527243463126457	25.357064654267507	29.0350506899751	21.080641192630935
20	25.09774553203593	27.501992157783413	28.75857530807902	18.64168700210164
21	24.97511213647353	26.097887748398936	30.456932144735628	18.470067970391906
22	23.821861589617754	22.76709023063882	31.558601042468513	21.852447137274908
23	22.450884563203886	24.047658283425925	30.966202168460928	22.535254984909265
24	22.403535543932442	24.44575122972006	30.827089734010254	22.323623492337248
25	22.65450227659051	25.76627530830476	28.171086284699097	23.408136130405634
26	21.612598112359958	26.12887059891148	28.57375047123279	23.684780817495774
27	21.642960177160955	25.335957940074994	30.443105553887584	22.577976328876467
28	19.076801350829708	26.465731500021445	30.213527710745463	24.243939438403384
29	20.928548693302513	25.83597261319181	30.113863386510214	23.121615306995466
30	24.208723958039403	24.54648782018434	29.919331718538018	21.325456503238243
31	24.20341906195893	25.85352391830911	28.897179826947518	21.045877192784438
32	24.74440559203769	25.933435969904306	27.850873727671466	21.471284710386534
33	25.403002796921804	24.21515755541359	28.32250156325129	22.059338084413312
34	22.41228297895875	25.328790686859886	29.924128699036316	22.334797635145048
35	22.745701341122874	26.839218758112544	28.7778196651369	21.637260235627682
36	25.545049854736146	27.092837938810842	26.973985692582403	20.38812651387061
37	23.954991907211724	26.972010465318398	28.021307623022796	21.05169000444708
38	23.32534589051146	26.210532137511937	29.153507890750745	21.31061408122586
39	23.642905999498858	24.07288475734051	29.262991916241333	23.0212173269193
40	26.495867824563703	23.39425310449291	28.626686561908137	21.483192509035256
41	23.57507105174644	24.34405524315612	27.97757044789125	24.103303257206193
42	22.958122924600495	24.565280696724734	29.81757929690939	22.659017081765377
43	22.107251454331617	23.91797050477779	30.892385103851804	23.082392937038783
44	22.693555341353132	24.078641133938465	28.639102276139027	24.58870124856937
45	23.03380234634425	23.581956129638115	27.802678182429734	25.581563341587906
46	23.64025355145862	24.26408675649624	28.347502296907134	23.748157395138005
47	21.560790722978325	25.727335113671508	30.548074774203304	22.16379938914686
48	21.82716422829564	25.24464600541325	30.85350134428324	22.074688422007867
49	24.41047931429139	23.199721436520708	30.308902970064583	22.080896279123316
50	22.795194892852386	23.5293022142862	29.34984548079289	24.325657412068526
51	22.065376636334697	22.980132599827986	28.155961687363284	26.798529076474026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	366.0
1	1048.0
2	1730.0
3	9876.0
4	18022.0
5	11614.0
6	5206.0
7	5043.5
8	4881.0
9	5083.5
10	5286.0
11	5276.0
12	5266.0
13	5265.5
14	5265.0
15	5105.5
16	4946.0
17	5153.0
18	5360.0
19	5349.0
20	5338.0
21	5855.0
22	6372.0
23	7147.5
24	7923.0
25	9563.0
26	12537.0
27	13871.0
28	17126.0
29	20381.0
30	23344.5
31	26308.0
32	31214.5
33	36121.0
34	40901.0
35	45681.0
36	49885.5
37	54090.0
38	59247.5
39	64405.0
40	71405.5
41	78406.0
42	87309.0
43	96212.0
44	105517.0
45	114822.0
46	130927.5
47	147033.0
48	165627.5
49	184222.0
50	179805.0
51	175388.0
52	156192.0
53	136996.0
54	122586.0
55	108176.0
56	99235.5
57	90295.0
58	84683.0
59	79071.0
60	70746.0
61	62421.0
62	56247.5
63	50074.0
64	42854.0
65	35634.0
66	29888.5
67	24143.0
68	20234.0
69	16325.0
70	13346.5
71	10368.0
72	8356.0
73	6344.0
74	5134.0
75	3025.0
76	2126.0
77	1594.0
78	1062.0
79	816.5
80	571.0
81	385.0
82	199.0
83	125.5
84	52.0
85	49.5
86	47.0
87	28.0
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1771948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.841029927802495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87699333289386	26.47827529020314
2	10.881870111984552	7.800348647081957
3	3.9102048597092773	4.204373082020369
4	1.8454339622370408	2.645690154812837
5	1.0899646536584615	1.9532727886009904
6	0.793977589188951	1.7074184721675398
7	0.6001934078990444	1.5058084926485595
8	0.5147711539377786	1.475994266740268
9	0.42291517188080247	1.364194379907142
>10	5.914042089563509	43.710787796889015
>50	0.10534342727420917	2.4842844818034724
>100	0.04059939437434355	2.5098884547689573
>500	0.0023990495088532376	0.6606247996830359
>1k	0.001107253619470725	0.9728804744230853
>5k	1.8454226991178752E-4	0.5261584182497548
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7955	0.448940939576105	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	3790	0.21388889515945161	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	3367	0.19001686279732816	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	2374	0.1339768435642581	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	2268	0.12799472670755574	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	1843	0.1040098242160605	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1287012937174229E-4	0.0	0.0	0.22675608990783025	0.0
2	1.1287012937174229E-4	0.0	0.0	0.7215223020088626	0.0
3	1.6930519405761342E-4	0.0	0.0	1.0693316056678863	0.0
4	1.6930519405761342E-4	0.0	0.0	1.4700770000022574	0.0
5	1.6930519405761342E-4	0.0	0.0	2.18189247088515	0.0
6	1.6930519405761342E-4	0.0	0.0	2.90031084433629	0.0
7	1.6930519405761342E-4	0.0	0.0	3.6550733994451305	0.0
8	1.6930519405761342E-4	0.0	0.0	4.744947368658674	0.0
9	1.6930519405761342E-4	0.0	0.0	5.329276028416184	0.0
10	1.6930519405761342E-4	0.0	0.0	6.305884822805184	0.0
11	1.6930519405761342E-4	0.0	0.0	8.057911405978054	0.0
12	1.6930519405761342E-4	0.0	0.0	9.46116928939224	0.0
13	1.6930519405761342E-4	0.0	0.0	9.974446202710238	0.0
14	1.6930519405761342E-4	0.0	0.0	10.204983441952022	0.0
15	1.6930519405761342E-4	0.0	0.0	10.478580635549125	0.0
16	1.6930519405761342E-4	0.0	0.0	11.058338055067079	0.0
17	1.6930519405761342E-4	0.0	0.0	11.843124064588803	0.0
18	1.6930519405761342E-4	0.0	0.0	12.645461379227832	0.0
19	1.6930519405761342E-4	0.0	0.0	13.241810707763433	0.0
20	1.6930519405761342E-4	0.0	0.0	13.908534561962314	0.0
21	1.6930519405761342E-4	0.0	0.0	14.855909992844033	0.0
22	1.6930519405761342E-4	0.0	0.0	15.885906358425869	0.0
23	1.6930519405761342E-4	0.0	0.0	16.878881321573772	0.0
24	1.6930519405761342E-4	0.0	0.0	17.64323783767921	0.0
25	1.6930519405761342E-4	0.0	0.0	18.326102120378252	0.0
26	1.6930519405761342E-4	0.0	0.0	18.92216927359042	0.0
27	1.6930519405761342E-4	0.0	0.0	19.54938858250919	0.0
28	1.6930519405761342E-4	0.0	0.0	20.180727651150033	0.0
29	1.6930519405761342E-4	0.0	0.0	20.856537550763342	0.0
30	1.6930519405761342E-4	0.0	0.0	21.68133602114735	0.0
31	1.6930519405761342E-4	0.0	0.0	22.351445979227382	0.0
32	1.6930519405761342E-4	0.0	0.0	23.020314365884325	0.0
33	2.2574025874348458E-4	0.0	0.0	23.67529972662855	0.0
34	2.2574025874348458E-4	0.0	0.0	24.358051139198217	0.0
35	2.2574025874348458E-4	0.0	0.0	25.059538993243596	0.0
36	2.2574025874348458E-4	0.0	0.0	25.70267299040378	0.0
37	3.3861038811522685E-4	0.0	0.0	26.33305266294496	0.0
38	3.3861038811522685E-4	0.0	0.0	27.05846898441715	0.0
39	3.3861038811522685E-4	0.0	0.0	28.136999505628832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGTA	20	7.034321E-4	45.000004	9
GCGATCG	30	2.1661708E-6	45.000004	9
CGTTTAT	1615	0.0	42.910217	1
TAGGGCG	710	0.0	42.78169	5
TTAGGGA	3265	0.0	41.69219	4
TTTAGGG	6100	0.0	41.64344	3
CGTTTTT	4045	0.0	41.050674	1
TCGAGGG	840	0.0	40.982143	3
CTAGGGA	2035	0.0	40.687958	4
TGTTAGG	1550	0.0	40.35484	2
TTAGGGG	5785	0.0	40.0605	4
TGTAGGG	2190	0.0	39.760273	3
GTTAGGG	2475	0.0	39.727272	3
GCTAGGG	1965	0.0	39.61832	3
TAGGGAG	2130	0.0	39.61268	5
TTTTAGG	3575	0.0	39.524475	2
TAGGGAC	1390	0.0	39.496403	5
TAGGGGA	3190	0.0	39.4279	5
TTCGTAG	355	0.0	39.295773	1
TAGGGAA	1605	0.0	39.25234	5
>>END_MODULE