FastQCFastQC Report
Sat 14 Jan 2017
SRR2934222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422685
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65110.4576557706027687No Hit
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT48760.3427322281460759RNA PCR Primer, Index 46 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC41760.2935294882563603RNA PCR Primer, Index 46 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC40750.2864302357865585RNA PCR Primer, Index 40 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC33020.23209635302262974RNA PCR Primer, Index 40 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.1677813430239301No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC20930.14711619227024958No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG19780.13903288500265343No Hit
CGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG18550.13038726070774628RNA PCR Primer, Index 46 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG16170.11365832914524297RNA PCR Primer, Index 46 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC15070.10592647001971625No Hit
TCTGTATCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC14490.10184967157171124TruSeq Adapter, Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACA351.2121927E-745.00000429
ACACGAT207.033854E-445.044
TCGATAG406.8175723E-945.01
GCGATCG253.891276E-545.09
CGTCGTA207.033854E-445.018
ATTGCGA207.033854E-445.013
TACGTAC900.045.024
CGTTTAT9200.043.043481
CGTTTTT26500.042.9622651
TTAGGGA51400.042.7237364
TAGGGAC21850.042.3226555
TTCTTCG3000.042.01
TAGGGAT32650.041.7611055
CTTTAGG16200.041.6666642
TAGGGAA19800.041.5909085
TCGTTAG1900.041.447371
TGTAGGG18850.041.4190983
GTAGGGA14850.041.212124
GTTAGGG20200.041.1014863
TTTAGGG61700.041.0980573
Produced by FastQC (version 0.11.5)