##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934222.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1422685
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21085904469366	33.0	31.0	34.0	30.0	34.0
2	32.374755479955155	34.0	31.0	34.0	30.0	34.0
3	32.41849320123569	34.0	31.0	34.0	30.0	34.0
4	35.949672626055666	37.0	35.0	37.0	35.0	37.0
5	35.05451593290152	37.0	35.0	37.0	35.0	37.0
6	25.916735609077204	36.0	0.0	37.0	0.0	37.0
7	31.027725743927856	37.0	19.0	37.0	17.0	37.0
8	34.7538632937017	37.0	32.0	37.0	32.0	37.0
9	37.28623553351585	39.0	37.0	39.0	35.0	39.0
10	37.22393432137121	39.0	37.0	39.0	34.0	39.0
11	37.27386807339643	39.0	37.0	39.0	34.0	39.0
12	37.029118181466735	39.0	37.0	39.0	34.0	39.0
13	37.00585653183944	39.0	37.0	39.0	33.0	39.0
14	37.723668978023944	40.0	37.0	41.0	32.0	41.0
15	37.93951155737215	40.0	37.0	41.0	33.0	41.0
16	38.16089085075052	40.0	37.0	41.0	33.0	41.0
17	38.239306663105324	40.0	37.0	41.0	34.0	41.0
18	38.23383320974074	40.0	37.0	41.0	34.0	41.0
19	38.254828018851676	40.0	37.0	41.0	34.0	41.0
20	38.11188913919806	40.0	37.0	41.0	33.0	41.0
21	38.028371705612976	40.0	37.0	41.0	33.0	41.0
22	38.115637685081374	40.0	37.0	41.0	34.0	41.0
23	38.071734783174065	40.0	36.0	41.0	34.0	41.0
24	38.00373659664648	40.0	36.0	41.0	34.0	41.0
25	37.871225886264355	40.0	36.0	41.0	34.0	41.0
26	37.85060501797657	40.0	36.0	41.0	34.0	41.0
27	37.840290015006836	40.0	36.0	41.0	34.0	41.0
28	37.79219995993491	40.0	36.0	41.0	34.0	41.0
29	37.78018113637242	40.0	36.0	41.0	34.0	41.0
30	37.488193802563465	39.0	36.0	41.0	33.0	41.0
31	37.44789394700865	39.0	35.0	41.0	32.0	41.0
32	37.407030368633954	39.0	35.0	41.0	33.0	41.0
33	37.30561297827699	39.0	35.0	41.0	32.0	41.0
34	37.198117643751075	39.0	35.0	41.0	32.0	41.0
35	37.21503003124374	39.0	35.0	41.0	32.0	41.0
36	37.15695955183333	39.0	35.0	41.0	32.0	41.0
37	37.07879326765939	39.0	35.0	41.0	32.0	41.0
38	37.01039302445728	39.0	35.0	41.0	31.0	41.0
39	36.86197717695765	39.0	35.0	41.0	31.0	41.0
40	36.73354537371238	39.0	35.0	41.0	31.0	41.0
41	36.66456102369815	39.0	35.0	41.0	31.0	41.0
42	36.706689815384294	39.0	35.0	41.0	31.0	41.0
43	36.6828208633675	39.0	35.0	41.0	31.0	41.0
44	36.59136773073449	39.0	35.0	41.0	31.0	41.0
45	36.57579857804082	39.0	35.0	40.0	31.0	41.0
46	36.51621476293066	39.0	35.0	40.0	31.0	41.0
47	36.41332761644355	39.0	35.0	40.0	31.0	41.0
48	36.3737032442178	39.0	35.0	40.0	31.0	41.0
49	36.35573721519521	39.0	35.0	40.0	31.0	41.0
50	36.22589118462625	39.0	35.0	40.0	30.0	41.0
51	35.202177572688264	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	7.0
11	6.0
12	4.0
13	1.0
14	9.0
15	27.0
16	67.0
17	115.0
18	241.0
19	407.0
20	760.0
21	1202.0
22	2066.0
23	3382.0
24	5334.0
25	8042.0
26	10368.0
27	12071.0
28	14197.0
29	17187.0
30	22518.0
31	30890.0
32	44180.0
33	68971.0
34	98206.0
35	106086.0
36	137766.0
37	230250.0
38	312280.0
39	295902.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.502110446093127	4.1222055479603705	58.0881923967709	14.287491609175609
2	21.060389334251784	3.749037910711085	54.494072827084	20.69649992795313
3	20.072398317266295	3.867967961987369	54.48535691316068	21.574276807585658
4	18.4331036033978	4.146385180134745	53.32339906585084	24.097112150616613
5	17.16789029194797	7.30056196557917	52.49559811202058	23.03594963045228
6	15.387805452366477	30.7226828145373	40.99628519313833	12.893226539957897
7	83.85510495998763	1.1728527397139916	10.53768051255197	4.4343617877464085
8	86.08230212590982	1.2588872448925799	8.037337850613453	4.621472778584156
9	81.06741829709317	4.185044475762379	10.115310135412969	4.63222709173148
10	37.3715896350914	36.06841992429807	15.552494051740196	11.00749638887034
11	26.085254290303194	22.674590650776523	32.288946604483776	18.951208454436504
12	26.29457680371973	19.997891311147583	33.559009900294164	20.148521984838528
13	26.36268745365278	21.667762013376116	34.435662145872065	17.533888387099044
14	20.120476423101387	24.469576891581763	35.906121172290426	19.503825513026428
15	17.016697301229716	25.0243026390241	38.25224838948889	19.706751670257294
16	23.04937494947933	25.660775224311777	31.034206447667618	20.25564337854128
17	23.607685467970775	24.473231952259283	29.272326621845313	22.64675595792463
18	22.824729297068572	23.789595026305893	32.49363000242499	20.892045674200542
19	22.494227464266512	26.044345726566316	30.79494055254677	20.666486256620402
20	20.34048296003683	31.856876258623657	29.898255762870914	17.9043850184686
21	20.975690332013063	27.33640967607025	34.233087436783265	17.45481255513343
22	19.79377023023368	23.09499291831994	35.109739682361166	22.001497169085216
23	18.461008585878112	25.94080910391267	32.4804858419116	23.117696468297623
24	19.721442202595796	28.318918102039454	29.556296720637388	22.403342974727362
25	21.630719379202002	27.11809009021674	27.291213445000125	23.959977085581137
26	17.97158190323227	27.044988876666302	27.157171123614855	27.826258096486573
27	18.196508714156685	25.48983084800922	31.917465918316424	24.39619451951767
28	16.08001771298636	25.091288654902527	30.522357373557746	28.306336258553372
29	18.889986188088017	25.18821805248527	28.81804475340641	27.103751006020303
30	22.588696724854763	24.695979784702867	29.0864105546906	23.628912935751764
31	20.718149133504607	27.045410614436786	28.19752791376868	24.038912338289922
32	22.64991899120325	27.35356034540323	25.682424429863254	24.31409623353026
33	22.522273026003646	27.24580634504476	25.470290331310164	24.76163029764143
34	17.985077511887734	26.18464382488042	29.386687847274693	26.44359081595715
35	17.543377486934915	27.933379490189324	26.435507508689554	28.087735514186203
36	20.25255063489107	27.682867254522257	25.01439180141774	27.050190309168933
37	17.81181357784752	29.109114104668286	27.1153487947086	25.963723522775595
38	21.358065910584564	26.867085827150778	27.356442220168205	24.418406042096457
39	20.264499871721426	27.931903407992632	26.94616165911639	24.85743506116955
40	20.815781427371487	27.95341203428728	29.193531948393353	22.03727458994788
41	18.07617287031212	26.764462969666507	28.151699076042835	27.007665083978534
42	17.62610837957805	25.748988707971193	30.900515574424414	25.72438733802634
43	18.566724187012586	25.183157199239464	29.942678808028482	26.30743980571947
44	20.550367790480674	23.874153449287792	27.252272990858835	28.3232057693727
45	19.95023494308297	23.057809704888996	25.33955162246035	31.652403729567684
46	20.5266801857052	23.755574846153575	28.2269792680741	27.490765700067126
47	16.010009243086138	25.71250839082439	31.665758758966323	26.61172360712315
48	16.865012283112566	28.220723491145268	28.900424197907476	26.013840027834696
49	21.349349996661243	24.065411528201956	29.749733778032383	24.83550469710442
50	19.615445442947667	24.80724826648204	27.459767973936604	28.117538316633688
51	17.351838249507093	25.16368697216882	25.560120476423105	31.92435430190098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	129.0
1	440.0
2	751.0
3	6135.5
4	11520.0
5	7034.0
6	2548.0
7	2566.5
8	2585.0
9	3101.0
10	3617.0
11	4053.0
12	4489.0
13	4619.5
14	4750.0
15	4737.5
16	4725.0
17	4545.0
18	4365.0
19	4534.5
20	4704.0
21	4914.5
22	5125.0
23	5663.0
24	6201.0
25	7171.5
26	9396.5
27	10651.0
28	12573.0
29	14495.0
30	16883.5
31	19272.0
32	23233.5
33	27195.0
34	31214.5
35	35234.0
36	38196.0
37	41158.0
38	46008.0
39	50858.0
40	58145.5
41	65433.0
42	75463.0
43	85493.0
44	98715.0
45	111937.0
46	133713.0
47	155489.0
48	178132.0
49	200775.0
50	187255.0
51	173735.0
52	145916.0
53	118097.0
54	100866.0
55	83635.0
56	73661.5
57	63688.0
58	53433.0
59	43178.0
60	33897.0
61	24616.0
62	19023.5
63	13431.0
64	11068.5
65	8706.0
66	6742.0
67	4778.0
68	3822.0
69	2866.0
70	2245.0
71	1624.0
72	1361.5
73	1099.0
74	925.0
75	567.5
76	384.0
77	308.0
78	232.0
79	189.0
80	146.0
81	100.5
82	55.0
83	33.0
84	11.0
85	8.0
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1422685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.00126252313578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46008767624173	26.071122015117844
2	12.862649604195006	9.518685494958559
3	5.022406878380495	5.575061862148787
4	2.5669424489554986	3.799204457423339
5	1.5542059011057274	2.875379028090991
6	1.0720791583343952	2.380096942986804
7	0.7820155513390636	2.0254893898590023
8	0.6205800166079175	1.8369795288897213
9	0.5063851047760548	1.6863199379661982
>10	4.313676793908406	30.67395530191353
>50	0.14777427760996728	3.7398211561770096
>100	0.08323984123362153	5.541470813716716
>500	0.004692440722557162	1.1952278766122462
>1k	0.0030602874277546703	2.589672980937479
>5k	2.0401916185031136E-4	0.4915132132016885
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6511	0.4576557706027687	No Hit
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT	4876	0.3427322281460759	RNA PCR Primer, Index 46 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	4176	0.2935294882563603	RNA PCR Primer, Index 46 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	4075	0.2864302357865585	RNA PCR Primer, Index 40 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	3302	0.23209635302262974	RNA PCR Primer, Index 40 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2387	0.1677813430239301	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2093	0.14711619227024958	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1978	0.13903288500265343	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG	1855	0.13038726070774628	RNA PCR Primer, Index 46 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG	1617	0.11365832914524297	RNA PCR Primer, Index 46 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC	1507	0.10592647001971625	No Hit
TCTGTATCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1449	0.10184967157171124	TruSeq Adapter, Index 8 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.02896284138794E-5	0.0	0.0	0.3646625922112063	0.0
2	7.02896284138794E-5	0.0	0.0	1.258254638236855	0.0
3	7.02896284138794E-5	0.0	0.0	1.8430643466403316	0.0
4	7.02896284138794E-5	0.0	0.0	2.5377367442547016	0.0
5	7.02896284138794E-5	0.0	0.0	3.7205706112034638	0.0
6	7.02896284138794E-5	0.0	0.0	4.593427216847018	0.0
7	7.02896284138794E-5	0.0	0.0	5.666117236071232	0.0
8	7.02896284138794E-5	0.0	0.0	7.405644960057919	0.0
9	7.02896284138794E-5	0.0	0.0	8.216084375669949	0.0
10	7.02896284138794E-5	0.0	0.0	9.65146887750978	0.0
11	7.02896284138794E-5	0.0	0.0	12.605320221974647	0.0
12	7.02896284138794E-5	0.0	0.0	15.50350218073572	0.0
13	7.02896284138794E-5	0.0	0.0	16.369821850936784	0.0
14	7.02896284138794E-5	0.0	0.0	16.6960360164056	0.0
15	7.02896284138794E-5	0.0	0.0	17.044250835567958	0.0
16	7.02896284138794E-5	0.0	0.0	17.815679507410284	0.0
17	7.02896284138794E-5	0.0	0.0	19.044904529112205	0.0
18	7.02896284138794E-5	0.0	0.0	20.407047238144777	0.0
19	7.02896284138794E-5	0.0	0.0	21.441429409883426	0.0
20	7.02896284138794E-5	0.0	0.0	22.525998376309584	0.0
21	7.02896284138794E-5	0.0	0.0	23.9638430151439	0.0
22	7.02896284138794E-5	0.0	0.0	25.67876936918573	0.0
23	7.02896284138794E-5	0.0	0.0	27.21832310033493	0.0
24	1.405792568277588E-4	0.0	0.0	28.308093499263716	0.0
25	1.405792568277588E-4	0.0	0.0	29.21792244945297	0.0
26	1.405792568277588E-4	0.0	0.0	30.024285066616994	0.0
27	1.405792568277588E-4	0.0	0.0	30.957169014926002	0.0
28	1.405792568277588E-4	0.0	0.0	31.8621479807547	0.0
29	1.405792568277588E-4	0.0	0.0	32.8704527003518	0.0
30	1.405792568277588E-4	0.0	0.0	34.1532384189051	0.0
31	1.405792568277588E-4	0.0	0.0	35.102429561006126	0.0
32	1.405792568277588E-4	0.0	0.0	35.95651883586317	0.0
33	1.405792568277588E-4	0.0	0.0	36.77939951570446	0.0
34	1.405792568277588E-4	0.0	0.0	37.71572765580575	0.0
35	1.405792568277588E-4	0.0	0.0	38.760653271806476	0.0
36	1.405792568277588E-4	0.0	0.0	39.65354242154799	0.0
37	2.1086888524163817E-4	0.0	0.0	40.558802545890345	0.0
38	2.1086888524163817E-4	0.0	0.0	41.63690486650243	0.0
39	2.1086888524163817E-4	0.0	0.0	43.750654572164606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGACA	35	1.2121927E-7	45.000004	29
ACACGAT	20	7.033854E-4	45.0	44
TCGATAG	40	6.8175723E-9	45.0	1
GCGATCG	25	3.891276E-5	45.0	9
CGTCGTA	20	7.033854E-4	45.0	18
ATTGCGA	20	7.033854E-4	45.0	13
TACGTAC	90	0.0	45.0	24
CGTTTAT	920	0.0	43.04348	1
CGTTTTT	2650	0.0	42.962265	1
TTAGGGA	5140	0.0	42.723736	4
TAGGGAC	2185	0.0	42.322655	5
TTCTTCG	300	0.0	42.0	1
TAGGGAT	3265	0.0	41.761105	5
CTTTAGG	1620	0.0	41.666664	2
TAGGGAA	1980	0.0	41.590908	5
TCGTTAG	190	0.0	41.44737	1
TGTAGGG	1885	0.0	41.419098	3
GTAGGGA	1485	0.0	41.21212	4
GTTAGGG	2020	0.0	41.101486	3
TTTAGGG	6170	0.0	41.098057	3
>>END_MODULE