SRR2934221.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76127
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	289	0.3796287782258594	RNA PCR Primer, Index 21 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	255	0.33496656902281713	RNA PCR Primer, Index 21 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	187	0.24564215061673259	TruSeq Adapter, Index 21 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	165	0.21674307407358756	TruSeq Adapter, Index 21 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.185216808753793	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	126	0.1655128929289214	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCAGG	30	2.1387987E-6	45.000004	2
TTGCTAG	30	2.1387987E-6	45.000004	1
TAGGGCG	30	2.1387987E-6	45.000004	5
TAGGGAG	35	1.1920565E-7	45.000004	5
CTAGGGA	85	0.0	45.000004	4
GGGTGAG	20	6.9917954E-4	45.0	7
CTTTTAG	20	6.9917954E-4	45.0	1
TATGGGA	40	6.672053E-9	45.0	4
TCTTAGG	80	0.0	45.0	2
GTTAGGG	40	6.672053E-9	45.0	3
CAACATA	20	6.9917954E-4	45.0	20
TTTTTAG	55	1.8189894E-12	45.0	1
ATTTTGC	20	6.9917954E-4	45.0	33
GTGCTAG	20	6.9917954E-4	45.0	1
TGTCTCG	20	6.9917954E-4	45.0	1
GGGAATC	25	3.8564554E-5	45.0	7
CGAGGGT	20	6.9917954E-4	45.0	4
TAGGGTT	20	6.9917954E-4	45.0	5
CGGGAAT	20	6.9917954E-4	45.0	6
GCGATGG	20	6.9917954E-4	45.0	9