##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76127
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3221984315683	33.0	31.0	34.0	31.0	34.0
2	32.49477846230641	34.0	31.0	34.0	31.0	34.0
3	32.559315354604806	34.0	31.0	34.0	31.0	34.0
4	36.02855754200218	37.0	35.0	37.0	35.0	37.0
5	35.14261694274042	37.0	35.0	37.0	35.0	37.0
6	26.216979521063486	37.0	0.0	37.0	0.0	37.0
7	31.087170123609233	37.0	19.0	37.0	17.0	37.0
8	34.673243395904215	37.0	32.0	37.0	32.0	37.0
9	37.1431029726641	39.0	37.0	39.0	34.0	39.0
10	37.29675410826645	39.0	37.0	39.0	34.0	39.0
11	37.370880239599614	39.0	37.0	39.0	35.0	39.0
12	37.03114532294718	39.0	37.0	39.0	34.0	39.0
13	36.98871622420429	39.0	37.0	39.0	33.0	39.0
14	37.95641493819539	40.0	37.0	41.0	33.0	41.0
15	38.154518107898646	40.0	37.0	41.0	33.0	41.0
16	38.28917466864581	40.0	37.0	41.0	33.0	41.0
17	38.31674701485675	40.0	37.0	41.0	34.0	41.0
18	38.31770593876023	40.0	37.0	41.0	34.0	41.0
19	38.30761753385789	40.0	37.0	41.0	34.0	41.0
20	38.212237445321634	40.0	37.0	41.0	34.0	41.0
21	38.10908087800649	40.0	37.0	41.0	34.0	41.0
22	38.141710562612474	40.0	37.0	41.0	34.0	41.0
23	38.081928881999815	40.0	36.0	41.0	34.0	41.0
24	38.01800937906393	40.0	36.0	41.0	34.0	41.0
25	37.887017746660185	40.0	36.0	41.0	34.0	41.0
26	37.78943081955154	40.0	36.0	41.0	34.0	41.0
27	37.76361868982096	40.0	36.0	41.0	33.0	41.0
28	37.679062619044494	40.0	36.0	41.0	33.0	41.0
29	37.65570691082008	40.0	36.0	41.0	33.0	41.0
30	37.36511356023487	40.0	35.0	41.0	33.0	41.0
31	37.3504275749734	40.0	35.0	41.0	33.0	41.0
32	37.28481353527658	39.0	35.0	41.0	33.0	41.0
33	37.22717301351689	39.0	35.0	41.0	32.0	41.0
34	37.11932691423542	39.0	35.0	41.0	32.0	41.0
35	37.078671167916774	39.0	35.0	41.0	32.0	41.0
36	37.02091242266213	39.0	35.0	41.0	32.0	41.0
37	36.89141828786107	39.0	35.0	41.0	31.0	41.0
38	36.81111826290278	39.0	35.0	41.0	31.0	41.0
39	36.74602966096129	39.0	35.0	41.0	31.0	41.0
40	36.54699383924232	39.0	35.0	41.0	31.0	41.0
41	36.417184441788066	39.0	35.0	41.0	31.0	41.0
42	36.45552826198327	39.0	35.0	41.0	31.0	41.0
43	36.42322697597436	39.0	35.0	40.0	31.0	41.0
44	36.30558146255599	38.0	35.0	40.0	30.0	41.0
45	36.24159627990069	38.0	35.0	40.0	30.0	41.0
46	36.15411089363826	38.0	35.0	40.0	30.0	41.0
47	36.031368633993196	38.0	35.0	40.0	30.0	41.0
48	36.01071893020873	38.0	35.0	40.0	30.0	41.0
49	35.97152127366112	38.0	35.0	40.0	30.0	41.0
50	35.82370249714293	38.0	35.0	40.0	30.0	41.0
51	34.78878715830126	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	0.0
13	1.0
14	2.0
15	2.0
16	7.0
17	11.0
18	27.0
19	28.0
20	62.0
21	114.0
22	144.0
23	202.0
24	313.0
25	432.0
26	587.0
27	636.0
28	818.0
29	984.0
30	1155.0
31	1628.0
32	2297.0
33	3695.0
34	5514.0
35	6062.0
36	7408.0
37	11964.0
38	15964.0
39	16057.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.795407674018417	4.5581725274869624	53.08234923220409	17.56407056629054
2	21.641467547650635	4.546350177992039	51.73854217294783	22.073640101409488
3	22.04868180803132	4.392659634558041	50.175364850841355	23.383293706569287
4	20.250371090414703	4.853731264860037	49.65780866184139	25.238088982883866
5	18.310192178859012	8.114072536682123	48.24306750561561	25.33266777884325
6	16.85604319098349	30.895740013398665	38.91917453728638	13.329042258331473
7	82.6290278087932	2.5785857842815294	9.731107228709918	5.061279178215351
8	83.83096667411036	2.9069843813627227	8.136403641283644	5.125645303243265
9	78.29416632732145	5.266199902794015	10.139635083478924	6.299998686405611
10	43.07932796511093	23.61579991330277	17.084608614552	16.2202635070343
11	31.43694090138847	24.25420678602861	24.0256413624601	20.28321095012282
12	29.195948874906406	19.610650623300536	30.77751651844943	20.415883983343626
13	27.439673177716184	21.123911358650677	30.66586099544183	20.770554468191314
14	20.43427430478017	24.237130058980387	33.160376738870575	22.16821889736887
15	18.597869349902137	23.20333127536879	35.04669828050495	23.152101094224125
16	23.89034114046265	22.391529943384082	30.149618400830192	23.56851051532308
17	24.77964454135852	22.20894032340694	27.51586165223902	25.49555348299552
18	25.138255809371184	22.315341468861245	29.153913854480013	23.392488867287557
19	26.13396035572136	24.315945722279874	27.023263756617233	22.526830165381533
20	25.98683778422898	27.409460506784715	26.83016538153349	19.77353632745281
21	26.472867707909153	24.3120049391149	28.761149132370907	20.45397822060504
22	23.619740696467744	22.365258055617588	30.108896974792128	23.906104273122544
23	24.472263454490523	22.953748341587087	28.834710418117094	23.7392777858053
24	24.92019914090927	24.283105862571755	26.81965662642689	23.97703837009208
25	24.04140449512	24.55370630656666	26.097179712848266	25.30770948546508
26	22.05130899680797	25.08833922261484	25.951370735744216	26.908981044832974
27	22.253602532609982	23.85224690320123	29.791007132817526	24.10314343137126
28	19.33873658491731	23.941571321607313	29.562441709249015	27.157250384226362
29	21.02013740197302	24.078185137993092	27.954602177939496	26.947075282094396
30	24.800662051571713	22.790862637434813	28.600890616995283	23.807584693998187
31	24.486712992762094	25.25122492676711	27.200598999041077	23.061463081429718
32	25.473222378394	24.049286061449944	25.478476755947298	24.999014804208755
33	25.830520052018336	23.15866906616575	26.374348128784792	24.636462753031118
34	22.08020807335111	23.341258686142893	29.54930576536577	25.029227475140225
35	21.825370762016107	23.86932363024945	27.24526120824412	27.060044399490323
36	24.87685052609455	23.952080076713912	26.658084516662946	24.51298488052859
37	22.798744203764763	25.919844470424426	28.10435193820852	23.1770593876023
38	23.678852443942358	24.58129178872148	26.476808491074127	25.263047276262036
39	24.185899877835723	23.230916757523612	25.14482378131281	27.43835958332786
40	25.74119563361225	23.688047604660632	26.298159654261955	24.272597107465156
41	20.33969550882079	23.93763053844234	26.786816766718776	28.935857186018097
42	21.05954523362276	22.838152035414506	29.391674438766795	26.71062829219594
43	21.533752807808003	21.47464106033339	30.04453084976421	26.947075282094396
44	23.1455331222825	21.556083912409527	27.70764643293444	27.590736532373533
45	23.752413729688545	21.47464106033339	25.529706937091966	29.243238272886096
46	23.353081035637814	22.254916126998307	28.510252604200875	25.881750233163004
47	20.01655128929289	23.941571321607313	30.79984762305095	25.24202976604884
48	20.761359307473036	23.694615576602256	29.79888869914748	25.745136416777225
49	23.536984250003286	21.054290856069464	30.0918202477439	25.316904646183353
50	22.826329685919582	21.109461820379103	28.076766456053697	27.987442037647615
51	21.65328989714556	22.62797693328254	26.059085475586848	29.659647693985054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	53.0
2	93.0
3	225.0
4	357.0
5	255.5
6	154.0
7	161.0
8	168.0
9	173.5
10	179.0
11	157.0
12	135.0
13	162.0
14	189.0
15	171.0
16	153.0
17	166.0
18	179.0
19	189.0
20	199.0
21	195.5
22	192.0
23	233.5
24	275.0
25	327.5
26	422.5
27	465.0
28	543.0
29	621.0
30	722.0
31	823.0
32	989.0
33	1155.0
34	1257.5
35	1360.0
36	1521.5
37	1683.0
38	1847.0
39	2011.0
40	2284.0
41	2557.0
42	2943.0
43	3329.0
44	3980.0
45	4631.0
46	5396.0
47	6161.0
48	6937.5
49	7714.0
50	7449.5
51	7185.0
52	6462.0
53	5739.0
54	5438.0
55	5137.0
56	5170.0
57	5203.0
58	4997.5
59	4792.0
60	4199.5
61	3607.0
62	3201.5
63	2796.0
64	2394.0
65	1992.0
66	1808.5
67	1625.0
68	1359.0
69	1093.0
70	937.0
71	781.0
72	635.0
73	489.0
74	372.0
75	199.0
76	143.0
77	106.0
78	69.0
79	48.5
80	28.0
81	19.5
82	11.0
83	6.5
84	2.0
85	2.5
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	76127.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.98184612555336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58232132191918	49.87061095275001
2	13.545689826796735	17.875392436323512
3	5.6997810073661155	11.282462201321476
4	2.6518017121242283	6.99883090099439
5	1.2104320127413895	3.99332694050731
6	0.5454907425841131	2.159549174405927
7	0.25084610790364326	1.15859025050245
8	0.11347800119450527	0.5989990410760965
9	0.06768863229145929	0.40195988282738054
>10	0.300617161059128	3.2485189223271633
>50	0.019908421262193908	0.8840490233425723
>100	0.011945052757316344	1.527710273621711
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	289	0.3796287782258594	RNA PCR Primer, Index 21 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	255	0.33496656902281713	RNA PCR Primer, Index 21 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	187	0.24564215061673259	TruSeq Adapter, Index 21 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	165	0.21674307407358756	TruSeq Adapter, Index 21 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.185216808753793	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	126	0.1655128929289214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40195988282738054	0.0
2	0.0	0.0	0.0	1.2807545286166537	0.0
3	0.0	0.0	0.0	1.7969971232282895	0.0
4	0.0	0.0	0.0	2.489261365875445	0.0
5	0.0	0.0	0.0	3.496788261720546	0.0
6	0.0	0.0	0.0	4.066888226253497	0.0
7	0.0	0.0	0.0	4.7630932520656275	0.0
8	0.0	0.0	0.0	5.771933742299053	0.0
9	0.0	0.0	0.0	6.25533647720257	0.0
10	0.0	0.0	0.0	7.1814205209715345	0.0
11	0.0	0.0	0.0	9.00206234318967	0.0
12	0.0	0.0	0.0	10.548162938247927	0.0
13	0.0	0.0	0.0	11.061778344082914	0.0
14	0.0	0.0	0.0	11.261444691108279	0.0
15	0.0	0.0	0.0	11.526790757549884	0.0
16	0.0	0.0	0.0	12.137612148120903	0.0
17	0.0	0.0	0.0	13.13331669447108	0.0
18	0.0	0.0	0.0	14.290593350585207	0.0
19	0.0	0.0	0.0	14.876456447778056	0.0
20	0.0	0.0	0.0	15.567407096036886	0.0
21	0.0	0.0	0.0	16.211068346316026	0.0
22	0.0	0.0	0.0	16.887569456303282	0.0
23	0.0	0.0	0.0	17.497077252485976	0.0
24	0.0	0.0	0.0	17.98442077055447	0.0
25	0.0	0.0	0.0	18.453373967186412	0.0
26	0.0	0.0	0.0	18.838257122965572	0.0
27	0.0	0.0	0.0	19.263861704782798	0.0
28	0.0	0.0	0.0	19.63823610545536	0.0
29	0.0	0.0	0.0	20.125579623523848	0.0
30	0.0	0.0	0.0	20.62080470792229	0.0
31	0.0	0.0	0.0	20.999119891759822	0.0
32	0.0	0.0	0.0	21.424724473577047	0.0
33	0.0	0.0	0.0	21.809607629356208	0.0
34	0.0	0.0	0.0	22.249661749445007	0.0
35	0.0	0.0	0.0	22.681834303203857	0.0
36	0.0	0.0	0.0	23.054895109488093	0.0
37	0.0	0.0	0.0	23.473931719363694	0.0
38	0.0	0.0	0.0	23.90479067873422	0.0
39	0.0	0.0	0.0	24.5931141382164	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCAGG	30	2.1387987E-6	45.000004	2
TTGCTAG	30	2.1387987E-6	45.000004	1
TAGGGCG	30	2.1387987E-6	45.000004	5
TAGGGAG	35	1.1920565E-7	45.000004	5
CTAGGGA	85	0.0	45.000004	4
GGGTGAG	20	6.9917954E-4	45.0	7
CTTTTAG	20	6.9917954E-4	45.0	1
TATGGGA	40	6.672053E-9	45.0	4
TCTTAGG	80	0.0	45.0	2
GTTAGGG	40	6.672053E-9	45.0	3
CAACATA	20	6.9917954E-4	45.0	20
TTTTTAG	55	1.8189894E-12	45.0	1
ATTTTGC	20	6.9917954E-4	45.0	33
GTGCTAG	20	6.9917954E-4	45.0	1
TGTCTCG	20	6.9917954E-4	45.0	1
GGGAATC	25	3.8564554E-5	45.0	7
CGAGGGT	20	6.9917954E-4	45.0	4
TAGGGTT	20	6.9917954E-4	45.0	5
CGGGAAT	20	6.9917954E-4	45.0	6
GCGATGG	20	6.9917954E-4	45.0	9
>>END_MODULE