SRR2934220.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934220.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327684
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1379	0.4208322652311373	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1350	0.4119822756069872	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1180	0.36010302608610734	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1034	0.3155479059093517	TruSeq Adapter, Index 19 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1008	0.30761343245321715	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	836	0.2551238388203269	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	768	0.23437213901197496	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	593	0.1809670292110692	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.14587224277047398	No Hit
GCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	377	0.11504986511395125	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	372	0.11352400483392536	TruSeq Adapter, Index 16 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	359	0.10955676810585809	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	346	0.1055895313777908	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	341	0.1040636710977649	TruSeq Adapter, Index 16 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATG	20	7.025896E-4	45.000004	11
GTCGCTA	20	7.025896E-4	45.000004	19
TCGTTAG	40	6.7902874E-9	45.000004	1
CTCCGTA	20	7.025896E-4	45.000004	20
GACGTCG	20	7.025896E-4	45.000004	18
CATTGCC	20	7.025896E-4	45.000004	42
GCTATCG	20	7.025896E-4	45.000004	22
ACATTGC	20	7.025896E-4	45.000004	41
CGCGCGT	20	7.025896E-4	45.000004	14
CCAGCCG	20	7.025896E-4	45.000004	44
AGGCGTT	20	7.025896E-4	45.000004	36
GTAGGGC	40	6.7902874E-9	45.000004	4
TATTGAC	20	7.025896E-4	45.000004	16
CCGTATG	20	7.025896E-4	45.000004	22
AGACGAC	20	7.025896E-4	45.000004	38
GCCGATG	40	6.7902874E-9	45.000004	9
GTAGCCG	20	7.025896E-4	45.000004	31
TCGCTAT	20	7.025896E-4	45.000004	20
TGTTCCC	40	6.7902874E-9	45.000004	22
ACGTGGG	20	7.025896E-4	45.000004	3