##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934220.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327684
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.351509380989	33.0	31.0	34.0	31.0	34.0
2	32.51312239840822	34.0	31.0	34.0	31.0	34.0
3	32.57480682608855	34.0	31.0	34.0	31.0	34.0
4	36.045623222372775	37.0	35.0	37.0	35.0	37.0
5	35.17469574346016	37.0	35.0	37.0	35.0	37.0
6	26.030297481720194	37.0	0.0	37.0	0.0	37.0
7	31.079393562090306	37.0	19.0	37.0	17.0	37.0
8	34.752337617949	37.0	32.0	37.0	32.0	37.0
9	37.296822548552875	39.0	37.0	39.0	35.0	39.0
10	37.40615654105785	39.0	37.0	39.0	35.0	39.0
11	37.40395930225461	39.0	37.0	39.0	35.0	39.0
12	36.96873512286227	39.0	35.0	39.0	34.0	39.0
13	36.83019006115648	39.0	35.0	39.0	33.0	39.0
14	37.90991626078783	40.0	36.0	41.0	33.0	41.0
15	38.104442084447214	40.0	36.0	41.0	33.0	41.0
16	38.24539190195432	40.0	36.0	41.0	34.0	41.0
17	38.24270028442036	40.0	36.0	41.0	34.0	41.0
18	38.24514471258896	40.0	36.0	41.0	34.0	41.0
19	38.235174741519266	40.0	36.0	41.0	34.0	41.0
20	38.14556401899391	40.0	36.0	41.0	34.0	41.0
21	38.025848073143635	40.0	36.0	41.0	34.0	41.0
22	37.99690555535211	40.0	36.0	41.0	34.0	41.0
23	37.96496319625005	40.0	35.0	41.0	34.0	41.0
24	37.92219638432148	40.0	35.0	41.0	34.0	41.0
25	37.80356685099059	40.0	35.0	41.0	34.0	41.0
26	37.75990283321737	39.0	35.0	41.0	34.0	41.0
27	37.73755813527667	40.0	35.0	41.0	34.0	41.0
28	37.70446832924403	40.0	35.0	41.0	34.0	41.0
29	37.71284835390193	39.0	35.0	41.0	34.0	41.0
30	37.50302730679557	39.0	35.0	41.0	33.0	41.0
31	37.49310311153428	39.0	35.0	41.0	33.0	41.0
32	37.42402741665751	39.0	35.0	41.0	33.0	41.0
33	37.33139243905714	39.0	35.0	41.0	33.0	41.0
34	37.26932654630681	39.0	35.0	41.0	33.0	41.0
35	37.23027062657926	39.0	35.0	41.0	33.0	41.0
36	37.129249520879874	39.0	35.0	41.0	33.0	41.0
37	37.06493451007678	39.0	35.0	41.0	33.0	41.0
38	37.0109007458405	39.0	35.0	41.0	33.0	41.0
39	36.97367280672843	39.0	35.0	41.0	33.0	41.0
40	36.87387544097362	39.0	35.0	41.0	32.0	41.0
41	36.78807021398664	39.0	35.0	41.0	32.0	41.0
42	36.77626921058093	39.0	35.0	41.0	32.0	41.0
43	36.70060790273556	39.0	35.0	41.0	32.0	41.0
44	36.55433588457172	39.0	35.0	41.0	31.0	41.0
45	36.501345808766985	39.0	35.0	40.0	31.0	41.0
46	36.44524602971155	38.0	35.0	40.0	31.0	41.0
47	36.36972204929139	38.0	35.0	40.0	31.0	41.0
48	36.367189121226545	38.0	35.0	40.0	31.0	41.0
49	36.39851808449604	38.0	35.0	40.0	31.0	41.0
50	36.27964136179978	38.0	35.0	40.0	31.0	41.0
51	35.362114109935185	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	3.0
13	0.0
14	5.0
15	2.0
16	12.0
17	34.0
18	53.0
19	106.0
20	158.0
21	274.0
22	477.0
23	617.0
24	1092.0
25	1691.0
26	2281.0
27	2769.0
28	3021.0
29	3587.0
30	4533.0
31	6071.0
32	8990.0
33	15969.0
34	24845.0
35	34331.0
36	28925.0
37	47484.0
38	67700.0
39	72608.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.284981872779873	4.351143174521796	53.663590532342134	17.700284420356198
2	19.90271114854555	5.709463995800832	53.49940796621135	20.88841688944227
3	20.54113108970838	5.454340156980505	51.5392878504901	22.46524090282101
4	18.854750308223778	4.510748159812502	52.26742837611845	24.367073155845265
5	18.65028503070031	7.867945947925441	48.18209006237717	25.299678958997085
6	16.37156528850966	31.66648356343306	39.1392316988318	12.822719449225472
7	83.03426471844826	1.5844533147788724	10.736868446430098	4.64441352034277
8	83.85883961377425	2.629667606596599	8.464252145359554	5.047240634269602
9	79.19336922156712	4.226938147727688	10.493646317793972	6.08604631291122
10	45.13067467438142	23.63160850087279	17.353914136790323	13.883802687955468
11	33.577165806081474	23.237326204514105	24.75403132285983	18.431476666544597
12	31.94998840346187	20.117552275973193	28.99531255721976	18.937146763345176
13	24.798281270980578	26.651896339156018	29.7524444281686	18.797377961694803
14	18.837050328975476	29.760684073680743	30.589531377790795	20.812734219552983
15	16.39567388093407	24.18396992224216	39.247262606657635	20.173093590166136
16	19.282296358687027	23.03713333577471	36.0081664042187	21.672403901319566
17	18.92219333260092	23.027673002038547	29.709109996215865	28.341023669144665
18	21.582378144798035	22.40725821218003	33.36232467865383	22.648038964368112
19	22.9895264950379	24.61914527410554	29.66577556426313	22.725552666593426
20	26.853615068175436	25.0057982690641	29.61603251913429	18.524554143626176
21	24.619450446161544	25.428461566631267	29.576360151853613	20.375727835353572
22	21.875343318563008	24.206857826442548	29.439948242819302	24.477850612175146
23	20.52526214279611	26.19596928748428	28.364521917457065	24.914246652262545
24	22.550689078502458	23.43629838502948	31.280440912586517	22.732571623881544
25	19.725406184006545	23.95997363313436	30.762869105601737	25.551751077257357
26	18.74763491656596	29.183908887830963	28.776809365120055	23.291646830483025
27	20.28417621855202	28.309285775320127	30.195859425544118	21.210678580583732
28	17.52572600432124	24.655460748770157	33.83473102134984	23.98408222555877
29	17.64230172971521	22.550689078502458	35.74114085521417	24.06586833656816
30	21.51005236752481	24.27246981848366	30.871510357539584	23.345967456451948
31	23.293172690763054	24.53583330281613	27.181980200437007	24.989013805983813
32	23.6084154246164	24.638371113633866	29.08961072252536	22.663602739224377
33	23.64442572722501	24.28406635661186	27.354707584135934	24.716800332027198
34	18.554461005114682	23.997814968079005	29.68622209201548	27.761501934790832
35	18.819960693839185	23.78083763625932	31.256942664274117	26.142259005627373
36	24.266366377363557	22.689542363984817	30.709463995800835	22.334627262850795
37	21.229599248056054	25.025024108592426	30.39910401484357	23.346272628507954
38	18.866346846351973	25.43822707242343	28.830519646976967	26.864906434247626
39	18.989026012866052	24.067394196848184	31.56486126878334	25.378718521502424
40	22.34561345686698	22.44815126768472	28.865309261361556	26.34092601408674
41	17.73721023913282	22.977319612797693	30.75981738504169	28.525652763027797
42	21.294600895985155	21.756021044664983	32.016210739615	24.933167319734867
43	22.1402326631755	21.55247128330953	31.178818617936795	25.128477435578176
44	20.232602141087145	23.050560906238935	30.01061998754898	26.706216965124934
45	20.081541973364583	23.498553484454536	27.64706241378889	28.77284212839199
46	22.655668265768238	23.06124192819912	29.900758047387114	24.382331758645527
47	17.97524444281686	23.53700516351119	32.220370845082456	26.267379548589492
48	19.497442658170677	21.704141795144103	33.322652311373155	25.475763235312073
49	20.28722793911207	19.090037963403766	34.83294881654277	25.789785280941395
50	20.297908961072253	21.52195407770901	31.174241037096717	27.00589592412202
51	18.107383943067102	22.37796169480353	27.94002758755386	31.574626774575503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	183.5
2	325.0
3	1460.5
4	2596.0
5	1658.5
6	721.0
7	716.5
8	712.0
9	848.5
10	985.0
11	1008.5
12	1032.0
13	1018.0
14	1004.0
15	921.5
16	839.0
17	833.5
18	828.0
19	805.0
20	782.0
21	804.0
22	826.0
23	1017.0
24	1208.0
25	1539.0
26	1935.0
27	2000.0
28	2618.0
29	3236.0
30	3841.5
31	4447.0
32	4740.5
33	5034.0
34	6146.0
35	7258.0
36	7372.5
37	7487.0
38	8893.0
39	10299.0
40	12784.0
41	15269.0
42	18741.5
43	22214.0
44	24419.0
45	26624.0
46	30316.5
47	34009.0
48	36676.0
49	39343.0
50	37356.5
51	35370.0
52	30246.0
53	25122.0
54	21641.0
55	18160.0
56	16409.5
57	14659.0
58	13678.0
59	12697.0
60	11171.0
61	9645.0
62	8364.0
63	7083.0
64	6008.0
65	4933.0
66	4097.5
67	3262.0
68	2681.5
69	2101.0
70	1789.0
71	1477.0
72	1242.0
73	1007.0
74	833.5
75	434.0
76	208.0
77	198.0
78	188.0
79	132.5
80	77.0
81	46.5
82	16.0
83	16.5
84	17.0
85	9.5
86	2.0
87	4.0
88	6.0
89	3.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	327684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.005900776253156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.16562866063643	21.817023472632478
2	12.991168028326122	8.315880697644765
3	4.801168724023843	4.609971893734713
4	2.546888110571796	3.2606179262071913
5	1.5449017235348377	2.472298563625925
6	1.0893186334357305	2.091877445728061
7	0.8820082757444996	1.976062855011881
8	0.7245409866213005	1.855166954090393
9	0.6487978530614983	1.868882373803915
>10	6.376357041210125	42.8130019112419
>50	0.1754046324293757	3.5849748791455243
>100	0.04584439256676865	2.735221958241202
>500	0.002989851689137086	0.7007909786887371
>1k	0.00498308614856181	1.8982280902033113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1379	0.4208322652311373	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1350	0.4119822756069872	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1180	0.36010302608610734	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1034	0.3155479059093517	TruSeq Adapter, Index 19 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1008	0.30761343245321715	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	836	0.2551238388203269	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	768	0.23437213901197496	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	593	0.1809670292110692	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.14587224277047398	No Hit
GCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	377	0.11504986511395125	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	372	0.11352400483392536	TruSeq Adapter, Index 16 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	359	0.10955676810585809	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	346	0.1055895313777908	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	341	0.1040636710977649	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44188913709549443	0.0
2	0.0	0.0	0.0	1.5652274752505462	0.0
3	0.0	0.0	0.0	2.1703836623088097	0.0
4	0.0	0.0	0.0	2.8524432074803774	0.0
5	0.0	0.0	0.0	3.9919556646037035	0.0
6	0.0	0.0	0.0	4.591313582597869	0.0
7	0.0	0.0	0.0	5.390864369331429	0.0
8	0.0	0.0	0.0	6.667399079601079	0.0
9	0.0	0.0	0.0	7.172458832289645	0.0
10	0.0	0.0	0.0	8.473712479095715	0.0
11	0.0	0.0	0.0	10.75823049035046	0.0
12	0.0	0.0	0.0	13.065331233749587	0.0
13	3.0517205600517574E-4	0.0	0.0	13.704361519024426	0.0
14	3.0517205600517574E-4	0.0	0.0	13.906995764211862	0.0
15	3.0517205600517574E-4	0.0	0.0	14.247872950769644	0.0
16	3.0517205600517574E-4	0.0	0.0	14.962585905933766	0.0
17	3.0517205600517574E-4	0.0	0.0	16.01695535943165	0.0
18	3.0517205600517574E-4	0.0	0.0	17.195835011779643	0.0
19	3.0517205600517574E-4	0.0	0.0	18.033837477569854	0.0
20	3.0517205600517574E-4	0.0	0.0	18.897474396064503	0.0
21	3.0517205600517574E-4	0.0	0.0	20.086729898316673	0.0
22	3.0517205600517574E-4	0.0	0.0	21.35899219980225	0.0
23	3.0517205600517574E-4	0.0	0.0	22.450897816188768	0.0
24	3.0517205600517574E-4	0.0	0.0	23.285848561418927	0.0
25	3.0517205600517574E-4	0.0	0.0	23.999646000415034	0.0
26	3.0517205600517574E-4	0.0	0.0	24.62738491961768	0.0
27	3.0517205600517574E-4	0.0	0.0	25.250241085924245	0.0
28	3.0517205600517574E-4	0.0	0.0	25.871876564006786	0.0
29	3.0517205600517574E-4	0.0	0.0	26.528606828529924	0.0
30	3.0517205600517574E-4	0.0	0.0	27.303438678727066	0.0
31	3.0517205600517574E-4	0.0	0.0	27.981225815114563	0.0
32	3.0517205600517574E-4	0.0	0.0	28.636125047301668	0.0
33	3.0517205600517574E-4	0.0	0.0	29.210764028759414	0.0
34	3.0517205600517574E-4	0.0	0.0	29.862306368330465	0.0
35	3.0517205600517574E-4	0.0	0.0	30.5895313777908	0.0
36	3.0517205600517574E-4	0.0	0.0	31.20933582353731	0.0
37	3.0517205600517574E-4	0.0	0.0	31.851112657316193	0.0
38	3.0517205600517574E-4	0.0	0.0	32.42025854176585	0.0
39	3.0517205600517574E-4	0.0	0.0	33.236898963635696	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATG	20	7.025896E-4	45.000004	11
GTCGCTA	20	7.025896E-4	45.000004	19
TCGTTAG	40	6.7902874E-9	45.000004	1
CTCCGTA	20	7.025896E-4	45.000004	20
GACGTCG	20	7.025896E-4	45.000004	18
CATTGCC	20	7.025896E-4	45.000004	42
GCTATCG	20	7.025896E-4	45.000004	22
ACATTGC	20	7.025896E-4	45.000004	41
CGCGCGT	20	7.025896E-4	45.000004	14
CCAGCCG	20	7.025896E-4	45.000004	44
AGGCGTT	20	7.025896E-4	45.000004	36
GTAGGGC	40	6.7902874E-9	45.000004	4
TATTGAC	20	7.025896E-4	45.000004	16
CCGTATG	20	7.025896E-4	45.000004	22
AGACGAC	20	7.025896E-4	45.000004	38
GCCGATG	40	6.7902874E-9	45.000004	9
GTAGCCG	20	7.025896E-4	45.000004	31
TCGCTAT	20	7.025896E-4	45.000004	20
TGTTCCC	40	6.7902874E-9	45.000004	22
ACGTGGG	20	7.025896E-4	45.000004	3
>>END_MODULE