SRR2934217.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934217.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536830
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9387	1.7485982527056982	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3416	0.6363280740644152	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	2330	0.4340293947804706	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	2263	0.42154872119665443	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	2133	0.3973324888698471	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	1945	0.3623120913510795	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	1769	0.3295270383547864	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1673	0.31164428217499024	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1346	0.25073114393755935	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1043	0.1942886947450776	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	1009	0.18795521859806644	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	852	0.15870946109569137	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	807	0.1503269191364119	No Hit
CGTTCAGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	654	0.1218262764748617	No Hit
CGTTTATGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	650	0.12108116163403684	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	577	0.10748281578898347	No Hit
CTGTCACTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	575	0.10711025836857106	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	538	0.10021794609094126	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGT	537	0.10003166738073506	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTG	20	7.029925E-4	45.0	45
GACACCG	20	7.029925E-4	45.0	32
ACACCGC	20	7.029925E-4	45.0	33
CAACCCG	20	7.029925E-4	45.0	23
GGATACG	25	3.8880185E-5	45.0	9
TTCTTCG	50	2.1827873E-11	45.0	1
CGAGGGT	50	2.1827873E-11	45.0	4
TCGTCAC	20	7.029925E-4	45.0	25
CGCAGTC	20	7.029925E-4	45.0	3
TAGGGCG	205	0.0	45.0	5
GCGATAC	20	7.029925E-4	45.0	9
ACGTCCG	20	7.029925E-4	45.0	14
ATACGTC	20	7.029925E-4	45.0	12
GGCGATA	45	3.8380676E-10	45.0	8
AGGGCGC	20	7.029925E-4	45.0	6
CGTTTAT	1850	0.0	45.0	1
TCGCGAG	25	3.8880185E-5	45.0	1
CGTTAGG	120	0.0	44.999996	2
CTTTGCG	60	0.0	44.999996	1
GTCGTAG	30	2.1633314E-6	44.999996	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content