##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934217.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536830
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19131568653019	33.0	31.0	34.0	30.0	34.0
2	32.407367322988655	34.0	31.0	34.0	31.0	34.0
3	32.284548180988395	33.0	31.0	34.0	30.0	34.0
4	35.935702177598124	37.0	35.0	37.0	35.0	37.0
5	35.12464094778608	37.0	35.0	37.0	35.0	37.0
6	25.94729802730846	36.0	0.0	37.0	0.0	37.0
7	31.040683270309035	37.0	19.0	37.0	17.0	37.0
8	34.777160367341615	37.0	32.0	37.0	32.0	37.0
9	37.365855112419204	39.0	37.0	39.0	35.0	39.0
10	37.36921371756422	39.0	37.0	39.0	34.0	39.0
11	37.39297356705102	39.0	37.0	39.0	34.0	39.0
12	37.29029860477246	39.0	37.0	39.0	34.0	39.0
13	37.371814168358696	39.0	37.0	39.0	34.0	39.0
14	38.58788256990108	40.0	38.0	41.0	34.0	41.0
15	38.68765158430043	40.0	38.0	41.0	35.0	41.0
16	38.71865022446585	40.0	38.0	41.0	35.0	41.0
17	38.717538140565914	40.0	38.0	41.0	35.0	41.0
18	38.569301268558014	40.0	38.0	41.0	35.0	41.0
19	38.45496525902055	40.0	37.0	41.0	35.0	41.0
20	38.26179796211091	40.0	37.0	41.0	34.0	41.0
21	38.11667380735056	40.0	36.0	41.0	34.0	41.0
22	38.066082372445656	40.0	36.0	41.0	34.0	41.0
23	37.96163217405883	40.0	35.0	41.0	34.0	41.0
24	37.89073077138014	39.0	35.0	41.0	34.0	41.0
25	37.78087662761023	39.0	35.0	41.0	34.0	41.0
26	37.73880930648436	39.0	35.0	41.0	34.0	41.0
27	37.712473222435406	39.0	35.0	41.0	34.0	41.0
28	37.64908816571354	39.0	35.0	41.0	33.0	41.0
29	37.60994914591211	39.0	35.0	41.0	33.0	41.0
30	37.303623120913514	39.0	35.0	41.0	33.0	41.0
31	37.19283758359257	39.0	35.0	41.0	33.0	41.0
32	36.86624257213643	39.0	35.0	41.0	32.0	41.0
33	36.427319635638845	39.0	35.0	41.0	30.0	41.0
34	36.090363802321036	39.0	35.0	41.0	29.0	41.0
35	35.87536650336233	39.0	35.0	41.0	27.0	41.0
36	35.7036119441909	39.0	35.0	41.0	25.0	41.0
37	35.57870648063633	39.0	35.0	41.0	24.0	41.0
38	35.47080081217518	39.0	35.0	41.0	24.0	41.0
39	35.36297151798521	39.0	35.0	41.0	23.0	41.0
40	35.319659855075166	39.0	35.0	41.0	23.0	41.0
41	35.21310657005011	39.0	35.0	41.0	23.0	41.0
42	35.10988394836354	39.0	35.0	40.0	22.0	41.0
43	35.02332582009202	39.0	35.0	40.0	22.0	41.0
44	34.918611105936705	39.0	35.0	40.0	21.0	41.0
45	34.90065011269862	39.0	35.0	40.0	21.0	41.0
46	34.82281727921316	39.0	35.0	40.0	21.0	41.0
47	34.71545927016001	38.0	35.0	40.0	20.0	41.0
48	34.63204552651678	38.0	34.0	40.0	20.0	41.0
49	34.58819924370844	38.0	34.0	40.0	20.0	41.0
50	34.49946538010171	38.0	34.0	40.0	20.0	41.0
51	33.54198722128048	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	0.0
13	6.0
14	6.0
15	11.0
16	21.0
17	48.0
18	127.0
19	239.0
20	498.0
21	846.0
22	1443.0
23	2670.0
24	4877.0
25	8369.0
26	11107.0
27	10849.0
28	9241.0
29	8568.0
30	8919.0
31	10593.0
32	13601.0
33	19593.0
34	29176.0
35	41680.0
36	50561.0
37	81620.0
38	111133.0
39	110975.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.271035523350037	3.79244826108824	53.09390309781495	22.842613117746772
2	30.00502952517557	3.532589460350576	47.89672708306168	18.56565393141218
3	16.809604530298234	3.406851330961384	59.6790417823147	20.104502356425684
4	14.69254698880465	3.533148296481195	60.39695993144944	21.37734478326472
5	14.639084998975468	6.80140826704916	57.718830914814745	20.84067581916063
6	12.85304472551832	30.442225658029543	44.93750349272582	11.767226123726319
7	74.03181640370322	0.9135107948512565	20.030549708473817	5.0241230929717045
8	76.0829312817838	0.9310209936106403	18.227185514967495	4.75886220963806
9	71.9708287539817	2.705139429614589	19.968332619264945	5.355699197138759
10	33.50408881768902	23.49551999701954	27.954473483225602	15.04591770206583
11	21.213419518283256	23.502598588007377	35.31024719184844	19.973734701860923
12	19.556284112288807	19.801799452340592	39.778328334854606	20.863588100515994
13	19.065625989605646	21.60013412067135	41.36896224130544	17.965277648417562
14	17.516532235530804	24.111729970381685	39.9698228489466	18.40191494514092
15	15.307453011195351	24.994504778048917	40.9237561239126	18.774286086843134
16	16.548441778589126	25.049829554980164	39.03936069146658	19.362367974964144
17	16.91131270607082	25.51683028146713	35.59208688039044	21.979770132071604
18	17.472943017342548	24.46640463461431	37.95242441741333	20.10822793062981
19	17.685859583108247	27.535718942682042	35.16886165080193	19.609559823407782
20	18.51200566287279	27.452824916640278	35.28863886146452	18.74653055902241
21	18.395954026414323	27.463815360542444	36.57526591285882	17.564964700184415
22	18.026749622785612	24.59177020658309	35.92571205036976	21.455768120261535
23	17.159622226775703	25.436544157368257	35.04088817689026	22.36294543896578
24	17.67393774565505	25.226421772255648	36.10360821861669	20.996032263472607
25	17.59719091705009	26.274239517165583	33.922098243391765	22.206471322392563
26	17.020099472831248	27.06871821619507	34.18773168414582	21.72345062682786
27	17.144347372538792	25.53843861185105	36.1695508820297	21.147663133580462
28	16.514539053331596	23.559227315910064	36.99867742115754	22.927556209600805
29	16.59724680066315	21.626026861390013	37.53795428720451	24.238772050742323
30	18.33019764171153	22.945811523201012	37.247918335413445	21.47607249967401
31	17.58042583313153	24.707821843041557	36.368682823240135	21.34306950058678
32	18.532868878415886	25.21952945997802	34.270066874056965	21.97753478754913
33	19.277424883110108	24.798725853622187	34.48335599724307	21.440493266024628
34	17.20488795335581	24.25050760948531	34.063483784438276	24.481120652720602
35	18.40340517482257	23.861371383864537	32.110537786636364	25.624685654676526
36	18.87003334388913	25.464485963899186	33.181267812901666	22.48421287931002
37	17.624015051319788	26.720004470689044	35.12862544939739	20.52735502859378
38	19.81949592981018	26.011400257064622	33.82560587150494	20.343497941620253
39	19.29363113089805	25.77259095058026	33.34649702885457	21.58728088966712
40	20.307359871840248	23.988599742935378	32.40057373842743	23.303466646796938
41	19.27872883408155	25.12713521971574	30.791498239666186	24.80263770653652
42	19.175902986047724	26.499823035225305	31.35368738706853	22.97058659165844
43	19.01141888493564	25.78004209898851	33.321163124266526	21.887375891809324
44	18.984222193245532	24.355009965910995	31.50084756813144	25.159920272712032
45	19.713689622413057	23.065588733863606	30.54914963768791	26.671572006035433
46	20.092207961552074	23.197287781979398	31.873404988543857	24.83709926792467
47	17.702624667026807	24.03964010953188	33.961030493824865	24.296704729616454
48	17.828549075126205	23.787232457202467	34.66330123130227	23.720917236369054
49	19.600059609187266	22.090419685934094	34.88981614291303	23.419704561965613
50	18.882327738762736	21.416463312408023	34.19182981577036	25.509379133058886
51	17.664437531434533	21.733137119758585	32.09414526013822	28.508280088668663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	188.5
2	328.0
3	8925.5
4	17523.0
5	11178.5
6	4834.0
7	4876.5
8	4919.0
9	5203.0
10	5487.0
11	5818.5
12	6150.0
13	6180.5
14	6211.0
15	6007.0
16	5803.0
17	5472.5
18	5142.0
19	4835.5
20	4529.0
21	4313.0
22	4097.0
23	4055.0
24	4013.0
25	4136.5
26	4558.0
27	4856.0
28	5325.5
29	5795.0
30	6545.5
31	7296.0
32	8516.5
33	9737.0
34	10938.5
35	12140.0
36	13602.0
37	15064.0
38	16754.5
39	18445.0
40	21255.5
41	24066.0
42	27855.5
43	31645.0
44	37112.5
45	42580.0
46	52096.0
47	61612.0
48	66411.5
49	71211.0
50	64425.0
51	57639.0
52	46550.0
53	35461.0
54	29093.0
55	22725.0
56	18890.0
57	15055.0
58	12701.0
59	10347.0
60	8827.5
61	7308.0
62	5860.5
63	4413.0
64	3604.5
65	2796.0
66	2097.0
67	1398.0
68	1120.0
69	842.0
70	681.0
71	520.0
72	385.0
73	250.0
74	215.0
75	118.0
76	56.0
77	40.0
78	24.0
79	19.5
80	15.0
81	9.5
82	4.0
83	3.0
84	2.0
85	2.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	536830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.06898330025747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79225478172386	43.9407834606464
2	9.697808384714415	10.6809689597387
3	3.582040860865164	5.917780450434707
4	1.9570349807036203	4.310877066815494
5	1.2467188393894757	3.43277694732277
6	0.8913222502268826	2.945052606773526
7	0.6108565699688397	2.3547475175326618
8	0.4294257374994289	1.8918431013645438
9	0.31801351133271233	1.5761412670353636
>10	1.3911278786590764	12.116912877986518
>50	0.05225239417123574	1.960311304201396
>100	0.02422296391305624	2.3854551074356003
>500	0.0031143810745357997	1.090776884083829
>1k	0.0034604234161508883	3.6067669610507744
>5k	3.4604234161508883E-4	1.788805487577726
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9387	1.7485982527056982	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3416	0.6363280740644152	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	2330	0.4340293947804706	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	2263	0.42154872119665443	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	2133	0.3973324888698471	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	1945	0.3623120913510795	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	1769	0.3295270383547864	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1673	0.31164428217499024	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1346	0.25073114393755935	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1043	0.1942886947450776	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	1009	0.18795521859806644	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCC	852	0.15870946109569137	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	807	0.1503269191364119	No Hit
CGTTCAGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	654	0.1218262764748617	No Hit
CGTTTATGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	650	0.12108116163403684	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	577	0.10748281578898347	No Hit
CTGTCACTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	575	0.10711025836857106	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	538	0.10021794609094126	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGT	537	0.10003166738073506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41875454054356126	0.0
2	0.0	0.0	0.0	1.2426652757856305	0.0
3	0.0	0.0	0.0	2.041242106439655	0.0
4	0.0	0.0	0.0	2.855838906171414	0.0
5	0.0	0.0	0.0	4.404932660246261	0.0
6	0.0	0.0	0.0	6.066352476575452	0.0
7	0.0	0.0	0.0	7.763165247843824	0.0
8	0.0	0.0	0.0	9.707914982396662	0.0
9	0.0	0.0	0.0	10.686064489689473	0.0
10	0.0	0.0	0.0	12.104763146619973	0.0
11	0.0	0.0	0.0	14.810647691075387	0.0
12	0.0	0.0	0.0	16.934224987426187	0.0
13	0.0	0.0	0.0	17.836186502244658	0.0
14	0.0	0.0	0.0	18.172978410297485	0.0
15	0.0	0.0	0.0	18.512378220293204	0.0
16	0.0	0.0	0.0	19.218374531974742	0.0
17	0.0	0.0	0.0	20.329154480934374	0.0
18	0.0	0.0	0.0	21.539220982433918	0.0
19	0.0	0.0	0.0	22.428888102378778	0.0
20	0.0	0.0	0.0	23.277387627368068	0.0
21	0.0	0.0	0.0	24.57984836912989	0.0
22	0.0	0.0	0.0	25.92291786971667	0.0
23	0.0	0.0	0.0	27.36266602090047	0.0
24	0.0	0.0	0.0	28.37937522120597	0.0
25	0.0	0.0	0.0	29.254512601754744	0.0
26	0.0	0.0	0.0	30.0	0.0
27	0.0	0.0	0.0	30.753497382784122	0.0
28	0.0	0.0	0.0	31.570329527038353	0.0
29	1.8627871020621053E-4	0.0	0.0	32.399456066166195	0.0
30	1.8627871020621053E-4	0.0	0.0	33.309613844233745	0.0
31	1.8627871020621053E-4	0.0	0.0	34.127004824618595	0.0
32	1.8627871020621053E-4	0.0	0.0	34.92632677011344	0.0
33	1.8627871020621053E-4	0.0	0.0	35.699383417469214	0.0
34	1.8627871020621053E-4	0.0	0.0	36.43872361827767	0.0
35	1.8627871020621053E-4	0.0	0.0	37.26300691094015	0.0
36	1.8627871020621053E-4	0.0	0.0	38.04854423187974	0.0
37	1.8627871020621053E-4	0.0	0.0	38.8001788275618	0.0
38	1.8627871020621053E-4	0.0	0.0	39.55758806326025	0.0
39	1.8627871020621053E-4	0.0	0.0	40.36529255071438	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTG	20	7.029925E-4	45.0	45
GACACCG	20	7.029925E-4	45.0	32
ACACCGC	20	7.029925E-4	45.0	33
CAACCCG	20	7.029925E-4	45.0	23
GGATACG	25	3.8880185E-5	45.0	9
TTCTTCG	50	2.1827873E-11	45.0	1
CGAGGGT	50	2.1827873E-11	45.0	4
TCGTCAC	20	7.029925E-4	45.0	25
CGCAGTC	20	7.029925E-4	45.0	3
TAGGGCG	205	0.0	45.0	5
GCGATAC	20	7.029925E-4	45.0	9
ACGTCCG	20	7.029925E-4	45.0	14
ATACGTC	20	7.029925E-4	45.0	12
GGCGATA	45	3.8380676E-10	45.0	8
AGGGCGC	20	7.029925E-4	45.0	6
CGTTTAT	1850	0.0	45.0	1
TCGCGAG	25	3.8880185E-5	45.0	1
CGTTAGG	120	0.0	44.999996	2
CTTTGCG	60	0.0	44.999996	1
GTCGTAG	30	2.1633314E-6	44.999996	1
>>END_MODULE