##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934216.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	914086
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35780331391138	33.0	31.0	34.0	31.0	34.0
2	32.53094785392184	34.0	31.0	34.0	31.0	34.0
3	32.587112153561044	34.0	31.0	34.0	31.0	34.0
4	36.05686226460092	37.0	35.0	37.0	35.0	37.0
5	35.205352669223686	37.0	35.0	37.0	35.0	37.0
6	26.54969882483705	37.0	10.0	37.0	0.0	37.0
7	31.31812214605628	37.0	19.0	37.0	17.0	37.0
8	34.732260421885904	37.0	32.0	37.0	32.0	37.0
9	37.250246694512334	39.0	37.0	39.0	34.0	39.0
10	37.35281472421632	39.0	37.0	39.0	34.0	39.0
11	37.37565174392781	39.0	37.0	39.0	35.0	39.0
12	37.038996330761	39.0	37.0	39.0	34.0	39.0
13	37.01187962620585	39.0	37.0	39.0	33.0	39.0
14	37.94227239012522	40.0	37.0	41.0	33.0	41.0
15	38.1187065549631	40.0	37.0	41.0	33.0	41.0
16	38.30185124813202	40.0	37.0	41.0	33.0	41.0
17	38.28545016552053	40.0	37.0	41.0	34.0	41.0
18	38.268597265465175	40.0	37.0	41.0	34.0	41.0
19	38.288861223123426	40.0	37.0	41.0	34.0	41.0
20	38.173003415433556	40.0	37.0	41.0	34.0	41.0
21	38.106307283997346	40.0	37.0	41.0	34.0	41.0
22	38.14880984940148	40.0	37.0	41.0	34.0	41.0
23	38.11364576199614	40.0	36.0	41.0	34.0	41.0
24	38.04956535818293	40.0	36.0	41.0	34.0	41.0
25	37.924858273729164	40.0	36.0	41.0	34.0	41.0
26	37.889091398402336	40.0	36.0	41.0	34.0	41.0
27	37.849185962808754	40.0	36.0	41.0	34.0	41.0
28	37.77273910769884	40.0	36.0	41.0	34.0	41.0
29	37.735622249985234	40.0	36.0	41.0	33.0	41.0
30	37.48370722229637	40.0	35.0	41.0	33.0	41.0
31	37.477030607623355	40.0	35.0	41.0	33.0	41.0
32	37.377068459641656	40.0	35.0	41.0	33.0	41.0
33	37.29213006216045	40.0	35.0	41.0	33.0	41.0
34	37.199504204199606	40.0	35.0	41.0	32.0	41.0
35	37.14400504985308	40.0	35.0	41.0	32.0	41.0
36	37.09077373463766	40.0	35.0	41.0	32.0	41.0
37	37.00726080478204	39.0	35.0	41.0	32.0	41.0
38	36.88843500502141	39.0	35.0	41.0	31.0	41.0
39	36.802004406587564	39.0	35.0	41.0	31.0	41.0
40	36.71952310832898	39.0	35.0	41.0	31.0	41.0
41	36.61955111444656	39.0	35.0	41.0	31.0	41.0
42	36.62563259912087	39.0	35.0	41.0	31.0	41.0
43	36.555825163059055	39.0	35.0	41.0	31.0	41.0
44	36.44276577914988	39.0	35.0	41.0	31.0	41.0
45	36.38128578711412	39.0	35.0	41.0	31.0	41.0
46	36.3292972433666	39.0	35.0	40.0	31.0	41.0
47	36.197584253560386	38.0	35.0	40.0	31.0	41.0
48	36.11880720194817	38.0	35.0	40.0	30.0	41.0
49	36.03674052550854	38.0	35.0	40.0	30.0	41.0
50	35.88631704237894	38.0	35.0	40.0	30.0	41.0
51	34.87627750561764	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	16.0
11	11.0
12	14.0
13	18.0
14	15.0
15	19.0
16	50.0
17	112.0
18	214.0
19	390.0
20	678.0
21	1168.0
22	1763.0
23	2634.0
24	3799.0
25	5227.0
26	6670.0
27	7950.0
28	9092.0
29	10741.0
30	14136.0
31	19281.0
32	26946.0
33	43357.0
34	64518.0
35	73564.0
36	83394.0
37	138765.0
38	191971.0
39	207395.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.845708171878794	4.5700295158223625	52.52919309561682	17.05506921668202
2	21.149541728021216	7.39667821189691	50.58233032778098	20.8714497323009
3	20.716212697711157	7.543053935844111	49.15412772977597	22.58660563666876
4	19.96869003572968	4.744739554046337	51.20426305621134	24.08230735401264
5	20.16331067317517	8.215747752399665	47.29631566395284	24.32462591047232
6	17.234374008572498	31.564207306533522	38.253621650479275	12.947797034414704
7	82.31916909349886	2.384239557328304	11.155843104478134	4.1407482446947
8	83.35331686515272	4.667832129580805	7.744785501583001	4.234065503683461
9	78.68143697638953	4.322131615624788	9.548663911273119	7.447767496712563
10	43.14222075384592	23.243655410978835	18.486553781591667	15.12757005358358
11	33.18352977728573	23.727307933826793	24.784320074916362	18.304842213971114
12	29.21978894764825	21.446778530685297	29.240027743560233	20.093404778106216
13	27.27073820187597	21.555849230816357	31.67874795150566	19.494664615802016
14	21.34788192795864	25.05256616992274	32.41872208960645	21.18082981251217
15	19.51227783819028	23.745905746286454	36.26245232943071	20.479364086092556
16	24.568366652590676	24.172889640580863	29.738558516375925	21.52018519045254
17	24.39278142319213	23.933634253232192	29.236526978861942	22.437057344713736
18	25.094356548508568	22.863822441214506	31.22900908667237	20.812811923604563
19	25.460952251757497	24.965265850259165	27.736230507851563	21.837551390131782
20	24.76462827348849	27.78502241583396	29.203597910918667	18.246751399758885
21	24.555566981662558	26.628019683049516	30.59001013033785	18.22640320495008
22	22.930227571585167	23.282273221556835	31.518806764352586	22.26869244250541
23	21.844224722837897	25.500445253510062	30.93111589062736	21.724214133024685
24	22.37141800662082	25.43502471321079	29.51308739002676	22.680469890141627
25	23.238732460621865	26.989692435941475	26.608218482724823	23.163356620711838
26	21.478176014073075	25.379997068109567	28.166386970153795	24.97543994766357
27	21.76983347299926	25.23766910334476	29.967639806320197	23.024857617335787
28	19.769693442411327	25.903361390503736	29.812621569524094	24.514323597560843
29	22.629490004222795	25.656557479274376	28.279724227260893	23.434228289241933
30	23.65959001669427	24.668794839872835	29.24265331708395	22.428961826348942
31	24.686845657848387	25.540813446437205	28.00896195762762	21.763378938086788
32	25.411613349290985	26.967265662093066	25.67482709504357	21.946293893572378
33	24.14750909651827	26.4002511798671	25.71541408576436	23.736825637850266
34	22.355008172097595	24.994584754607335	30.30174403721313	22.348663036081945
35	21.580573381498024	27.532310964176236	26.816623381169823	24.070492273155917
36	25.020402894257217	27.302682679747857	25.100920482317857	22.575993943677073
37	21.174047080909236	27.84409782011758	27.80876197644423	23.17309312252895
38	22.800918075542125	27.86313322816453	26.211866279540434	23.12408241675291
39	21.086090367864728	27.54904899538993	27.311762788184048	24.053097848561293
40	23.067632585993003	25.37244854422888	28.475767050365064	23.084151819413055
41	20.1883630205473	25.208678395687055	27.790382961778214	26.812575621987428
42	21.15774664528283	24.374511807422934	28.998803176068773	25.468938371225462
43	21.715462221278962	25.638178464608362	28.164417790011004	24.48194152410167
44	22.54273667904333	25.009134807884596	27.511743971573793	24.93638454149828
45	22.271974409410056	23.80738792630015	26.15104049290767	27.76959717138212
46	22.189706439000272	25.09271556505624	27.544235443929782	25.173342552013704
47	19.123364759989762	25.86999472697317	31.525589495955526	23.481051017081544
48	20.44741960822067	27.115172970595765	28.353349684821776	24.084057736361785
49	22.037532573521528	24.2960727984019	29.919941887306006	23.746452740770565
50	20.928446557544913	25.281428662073374	28.033904906102926	25.756219874278784
51	20.261222685830436	24.969970002822492	26.47212625507884	28.29668105626823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	733.5
2	1291.0
3	2868.5
4	4446.0
5	3070.5
6	1695.0
7	1724.0
8	1753.0
9	1840.0
10	1927.0
11	1879.0
12	1831.0
13	1968.0
14	2105.0
15	2087.5
16	2070.0
17	2023.5
18	1977.0
19	2203.5
20	2430.0
21	2648.5
22	2867.0
23	3222.5
24	3578.0
25	4428.0
26	6363.5
27	7449.0
28	9026.0
29	10603.0
30	12992.5
31	15382.0
32	17917.5
33	20453.0
34	22637.0
35	24821.0
36	26489.5
37	28158.0
38	31474.5
39	34791.0
40	37670.5
41	40550.0
42	44561.0
43	48572.0
44	53654.5
45	58737.0
46	74516.5
47	90296.0
48	89096.5
49	87897.0
50	88083.0
51	88269.0
52	78975.0
53	69681.0
54	64194.0
55	58707.0
56	54660.5
57	50614.0
58	46426.5
59	42239.0
60	36990.5
61	31742.0
62	27688.0
63	23634.0
64	19967.0
65	16300.0
66	14129.0
67	11958.0
68	9776.0
69	7594.0
70	6333.5
71	5073.0
72	4197.5
73	3322.0
74	2609.0
75	1505.0
76	1114.0
77	810.5
78	507.0
79	333.5
80	160.0
81	115.5
82	71.0
83	59.0
84	47.0
85	30.5
86	14.0
87	9.5
88	5.0
89	3.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	914086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.00693864192718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.44847697475495	23.582954955648834
2	10.199549354101473	6.733117994122706
3	3.9150381178922977	3.8767026881423137
4	2.1641328053641566	2.857255948785458
5	1.5157541142564508	2.5015201522755675
6	1.1712332801453609	2.319529500788462
7	0.9357864862676569	2.162121299392683
8	0.8668835897714793	2.289053876582463
9	0.7197382625815202	2.1380721004147962
>10	6.914660519818167	43.238436487707794
>50	0.10252388135063327	2.3191292041020906
>100	0.038924306270337085	2.2476630594871208
>500	0.002780307590736766	0.6156582443739548
>1k	0.004170461386105149	2.517924066332832
>5k	3.4753844884209575E-4	0.6008604218428871
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	5238	0.5730314215511451	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	4515	0.4939360191491829	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	3869	0.42326433180247813	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1938	0.2120150620401144	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1767	0.1933078506836337	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1626	0.17788260623179877	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1428	0.1562216246611369	No Hit
TCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1187	0.12985649052714954	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT	1186	0.129747091630328	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTC	1175	0.12854370376529123	No Hit
GAATCAGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	1147	0.12548053465428854	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAATCAT	1075	0.11760381408313879	No Hit
CTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGCT	1037	0.11344665600392086	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	945	0.10338195749634062	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12011998871003385	0.0
2	0.0	0.0	0.0	0.6886660554914964	0.0
3	0.0	0.0	0.0	1.008329632003991	0.0
4	0.0	0.0	0.0	1.458724890218207	0.0
5	0.0	0.0	0.0	2.51168927212538	0.0
6	0.0	0.0	0.0	2.957270978879449	0.0
7	0.0	0.0	0.0	3.620775288101995	0.0
8	0.0	0.0	0.0	4.545524162934341	0.0
9	0.0	0.0	0.0	4.925028936008209	0.0
10	0.0	0.0	0.0	6.18235045717799	0.0
11	0.0	0.0	0.0	7.64774868010231	0.0
12	0.0	0.0	0.0	9.496808834179717	0.0
13	0.0	0.0	0.0	9.966895893821807	0.0
14	0.0	0.0	0.0	10.1615165312673	0.0
15	0.0	0.0	0.0	10.494964368779305	0.0
16	0.0	0.0	0.0	11.053117540362724	0.0
17	1.0939889682152446E-4	0.0	0.0	11.755567856853732	0.0
18	1.0939889682152446E-4	0.0	0.0	12.48317991961369	0.0
19	1.0939889682152446E-4	0.0	0.0	13.184645646033305	0.0
20	1.0939889682152446E-4	0.0	0.0	13.822003618915506	0.0
21	1.0939889682152446E-4	0.0	0.0	14.639979170450045	0.0
22	2.1879779364304892E-4	0.0	0.0	15.549193401933735	0.0
23	2.1879779364304892E-4	0.0	0.0	16.38401638357879	0.0
24	2.1879779364304892E-4	0.0	0.0	17.020499165286417	0.0
25	2.1879779364304892E-4	0.0	0.0	17.60851823570211	0.0
26	2.1879779364304892E-4	0.0	0.0	18.129038186778924	0.0
27	2.1879779364304892E-4	0.0	0.0	18.718807639543762	0.0
28	2.1879779364304892E-4	0.0	0.0	19.251689665961408	0.0
29	2.1879779364304892E-4	0.0	0.0	19.897252556105226	0.0
30	2.1879779364304892E-4	0.0	0.0	20.70483521244172	0.0
31	2.1879779364304892E-4	0.0	0.0	21.299746413357166	0.0
32	2.1879779364304892E-4	0.0	0.0	21.867636086757702	0.0
33	2.1879779364304892E-4	0.0	0.0	22.444824666388065	0.0
34	2.1879779364304892E-4	0.0	0.0	23.075728104357797	0.0
35	2.1879779364304892E-4	0.0	0.0	23.797432626689393	0.0
36	3.281966904645734E-4	0.0	0.0	24.402627323906067	0.0
37	3.281966904645734E-4	0.0	0.0	25.03725032436773	0.0
38	3.281966904645734E-4	0.0	0.0	25.729854740144802	0.0
39	3.281966904645734E-4	0.0	0.0	26.95840435145052	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAG	35	1.2115561E-7	45.000004	1
TCGTTGA	75	0.0	45.0	24
CTGTCGA	20	7.032528E-4	45.0	12
TACGCAC	20	7.032528E-4	45.0	28
CGAATAC	20	7.032528E-4	45.0	24
CGATGCA	25	3.8901762E-5	45.0	10
TTTAGGG	3005	0.0	40.956738	3
AGGGATC	370	0.0	40.743244	6
GTTAGGG	1215	0.0	40.37037	3
TAGGGAT	790	0.0	40.158226	5
TTAGGGA	1570	0.0	40.127388	4
TTAGGGG	3075	0.0	39.878048	4
CGTTGAT	85	0.0	39.705883	25
AATCGTT	85	0.0	39.705883	22
TACGGCT	575	0.0	39.52174	7
TAGGGCC	325	0.0	39.461536	5
AGGGCGA	565	0.0	39.42478	6
GTTTGCG	120	0.0	39.374996	1
TCAGGGA	755	0.0	39.33775	4
TAGGGCA	510	0.0	39.2647	5
>>END_MODULE