FastQCFastQC Report
Sat 14 Jan 2017
SRR2934215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215105
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT36800.30285448582632774Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC31970.2631048345616222Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC28630.23561749807629795Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC22410.18442850617847842Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.137601277255875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAG600.045.0000041
GCCCCGT351.2120108E-745.040
CTATTCG207.033447E-445.01
GACGAAT207.033447E-445.09
ACGAATT207.033447E-445.010
GCGTAGG850.042.3529432
TTAGGGA25300.041.798424
TTTAGGG47800.041.563813
AGGGCGA8750.041.46
CGAAGTA556.184564E-1140.90909224
CGTTTAT4950.040.909091
TAGGGCG6250.040.685
TTAGGGT11050.040.520364
CGTTGAT501.0822987E-940.49999625
CGTAGGG4400.040.3977283
CTTGTAG2350.040.2127651
TTTTAGG26850.039.804472
CTTAGGG22300.039.7533653
GTTAGGG19750.039.645573
TTGTAGG11400.039.4736862
Produced by FastQC (version 0.11.5)