##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934214.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 890012 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19688161507935 33.0 31.0 34.0 30.0 34.0 2 32.3590086425801 34.0 31.0 34.0 30.0 34.0 3 32.41440340130245 34.0 31.0 34.0 30.0 34.0 4 35.94044349963821 37.0 35.0 37.0 35.0 37.0 5 35.04918135935246 37.0 35.0 37.0 35.0 37.0 6 25.778589502164017 35.0 0.0 37.0 0.0 37.0 7 30.939832271924423 37.0 19.0 37.0 17.0 37.0 8 34.669137045343206 37.0 32.0 37.0 32.0 37.0 9 37.257634728520514 39.0 37.0 39.0 35.0 39.0 10 37.15860572666436 39.0 37.0 39.0 34.0 39.0 11 37.19572769805351 39.0 37.0 39.0 34.0 39.0 12 37.12764884068979 39.0 37.0 39.0 34.0 39.0 13 37.2157229340728 39.0 37.0 39.0 34.0 39.0 14 38.38284652341766 40.0 38.0 41.0 34.0 41.0 15 38.467317294598274 40.0 38.0 41.0 34.0 41.0 16 38.53165800011685 40.0 38.0 41.0 34.0 41.0 17 38.51696943411999 40.0 38.0 41.0 34.0 41.0 18 38.519124461243216 40.0 38.0 41.0 34.0 41.0 19 38.52028624333155 40.0 38.0 41.0 34.0 41.0 20 38.455681496429264 40.0 38.0 41.0 34.0 41.0 21 38.3761342543696 40.0 38.0 41.0 34.0 41.0 22 38.38166339330256 40.0 37.0 41.0 34.0 41.0 23 38.318511435800865 40.0 37.0 41.0 34.0 41.0 24 38.2694817597965 40.0 37.0 41.0 34.0 41.0 25 38.14377109522119 40.0 37.0 41.0 34.0 41.0 26 38.11343330202289 40.0 37.0 41.0 34.0 41.0 27 38.09565713720714 40.0 37.0 41.0 34.0 41.0 28 38.04960944346818 40.0 37.0 41.0 34.0 41.0 29 38.03136362206352 40.0 37.0 41.0 34.0 41.0 30 37.78732983375505 40.0 37.0 41.0 33.0 41.0 31 37.82601807616077 40.0 37.0 41.0 33.0 41.0 32 37.734633915048335 40.0 37.0 41.0 33.0 41.0 33 37.688110946818696 40.0 37.0 41.0 33.0 41.0 34 37.662996678696466 40.0 37.0 41.0 33.0 41.0 35 37.6084389873395 40.0 37.0 41.0 33.0 41.0 36 37.57049343154924 40.0 37.0 41.0 33.0 41.0 37 37.48516536855683 40.0 36.0 41.0 33.0 41.0 38 37.399756407778774 40.0 36.0 41.0 33.0 41.0 39 37.39443962553314 40.0 36.0 41.0 33.0 41.0 40 37.38359258077419 40.0 36.0 41.0 33.0 41.0 41 37.322131611708606 40.0 36.0 41.0 33.0 41.0 42 37.29691397419361 40.0 36.0 41.0 32.0 41.0 43 37.26788178136924 39.0 36.0 41.0 32.0 41.0 44 37.15485746259601 39.0 36.0 41.0 32.0 41.0 45 37.15391140793607 39.0 36.0 41.0 32.0 41.0 46 37.068881093738064 39.0 36.0 41.0 32.0 41.0 47 36.96633977968836 39.0 35.0 41.0 31.0 41.0 48 36.957320800168986 39.0 35.0 41.0 31.0 41.0 49 36.98785746709033 39.0 35.0 41.0 32.0 41.0 50 36.90271816559777 39.0 35.0 41.0 31.0 41.0 51 35.95405230491274 38.0 35.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 6.0 11 1.0 12 3.0 13 3.0 14 4.0 15 7.0 16 15.0 17 35.0 18 91.0 19 176.0 20 324.0 21 647.0 22 1074.0 23 1711.0 24 2663.0 25 4031.0 26 5151.0 27 5971.0 28 6815.0 29 8480.0 30 11306.0 31 15479.0 32 21785.0 33 33622.0 34 49861.0 35 68550.0 36 90396.0 37 152042.0 38 207909.0 39 201786.0 40 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.661366363599594 4.280167008984149 58.6656134973461 13.392853130070156 2 19.917708974710454 5.504083091014503 53.437144667712346 21.1410632665627 3 19.24490905740597 5.507229116011919 54.27612211970175 20.971739706880356 4 17.821220388039713 3.9438794083675273 54.7178015577318 23.517098645860955 5 17.858073823723725 7.351586270746911 52.12457809557623 22.665761809953125 6 14.423513390830687 32.10900527183903 41.24674723486874 12.22073410246154 7 82.07327541651124 1.3652624908428201 11.550181345869493 5.011280746776448 8 83.6522428911071 2.3163732623829794 8.402133903812532 5.6292499426974025 9 79.45443432223385 3.4602904230504756 10.544239852945802 6.5410354017698635 10 38.23577659627061 24.477422776322115 21.44971079041631 15.83708983699096 11 24.69809395828371 26.67098870577026 26.525934481782272 22.10498285416376 12 20.601632337541517 22.52576369756812 33.92864365873719 22.943960306153173 13 22.267789647780027 24.02900185615475 35.5248019127832 18.178406583282023 14 20.37556796987007 27.09087068488964 32.482820456353394 20.050740888886892 15 17.192914252841536 25.770663766331243 35.84524703037712 21.191174950450105 16 17.81189467108309 26.384138640827317 31.762268373909563 24.04169831418003 17 17.72515426758291 27.915691024390682 29.69768946935547 24.66146523867094 18 18.945137818366494 25.396511507710006 33.84459984809194 21.813750825831562 19 19.82669896585664 28.728151979973305 30.5071167579763 20.93803229619376 20 21.29072417001119 28.331528114227673 31.866424272931155 18.511323442829987 21 21.04825552913893 29.24084169651645 30.575992233812578 19.134910540532037 22 19.023114295088156 26.659415828101196 29.93139418344921 24.386075693361438 23 18.393347505426895 28.55073864172618 28.113778241192257 24.942135611654674 24 19.850631227444122 26.539417446056905 29.3381437553651 24.27180757113387 25 17.487517022242397 29.57589335874123 26.82491921457239 26.111670404443988 26 16.443598513278474 29.46398475526173 29.020507588661726 25.071909142798077 27 19.167157296755548 29.129719599286304 29.155786663550604 22.547336440407545 28 17.485719293672446 27.016152591201013 31.374970225120556 24.123157890005977 29 18.453683770555905 26.14346772852501 29.576679864990584 25.826168635928504 30 21.67937061522766 28.557255407792255 27.87670278602985 21.886671190950235 31 21.499934832339342 30.63846330161841 24.3005712282531 23.561030637789155 32 18.851094142550888 32.90337658368651 24.42124375851112 23.824285515251482 33 20.384219538612964 29.120393882329676 25.521678359392908 24.973708219664452 34 19.40647991263039 27.220756574068666 25.872347788569144 27.500415724731802 35 16.81730133975722 29.178370628710624 25.304827350642462 28.699500680889695 36 20.72488910261884 28.771409823687772 25.86246028143441 24.64124079225898 37 19.204460164582052 31.170815674395403 27.71299712812861 21.91172703289394 38 20.6955636553215 31.795975784596166 25.527071545102764 21.981389014979573 39 21.32802703783769 27.77063680040269 28.254787575897854 22.646548585861765 40 21.790380354422187 25.904706902828277 27.200869201763574 25.104043540985966 41 17.759311110411993 28.373100587407812 25.608418762893088 28.25916953928711 42 18.427841422362846 28.018723343056052 28.765230131728558 24.788205102852544 43 20.052201543349977 26.207961240972033 30.02948274854721 23.71035446713078 44 20.283996170838144 25.744933776173806 26.67739311380071 27.29367693918734 45 21.52263115553498 24.92618077059635 24.845620059055385 28.70556801481328 46 23.190698552379068 25.751787616346743 26.382453270292984 24.675060560981198 47 17.7797602728952 27.905017011006596 29.480501386498158 24.83472132960005 48 18.866374835395476 27.70692979420502 28.422987555223973 25.003707815175524 49 22.231947434416615 23.994845013325662 30.448128789274755 23.32507876298297 50 21.959479198033286 22.953510739181045 27.514685195255794 27.572324867529872 51 19.3753567367631 22.81002952769176 25.120447814186775 32.69416592135836 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49.0 1 230.5 2 412.0 3 2273.5 4 4135.0 5 2689.5 6 1244.0 7 1421.5 8 1599.0 9 1878.5 10 2158.0 11 2416.5 12 2675.0 13 2872.0 14 3069.0 15 3009.5 16 2950.0 17 2922.0 18 2894.0 19 2846.5 20 2799.0 21 2987.0 22 3175.0 23 3223.5 24 3272.0 25 3907.5 26 4989.5 27 5436.0 28 7089.0 29 8742.0 30 10219.5 31 11697.0 32 15234.5 33 18772.0 34 19989.0 35 21206.0 36 24462.5 37 27719.0 38 30995.0 39 34271.0 40 39412.5 41 44554.0 42 51952.5 43 59351.0 44 69453.0 45 79555.0 46 99717.5 47 119880.0 48 127451.0 49 135022.0 50 123536.5 51 112051.0 52 91141.0 53 70231.0 54 56024.5 55 41818.0 56 33444.0 57 25070.0 58 20615.0 59 16160.0 60 13135.0 61 10110.0 62 7926.0 63 5742.0 64 4805.5 65 3869.0 66 2891.0 67 1913.0 68 1420.0 69 927.0 70 674.0 71 421.0 72 349.5 73 278.0 74 214.5 75 94.5 76 38.0 77 29.0 78 20.0 79 17.0 80 14.0 81 13.0 82 12.0 83 6.5 84 1.0 85 3.0 86 5.0 87 2.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 890012.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.06063885292597 #Duplication Level Percentage of deduplicated Percentage of total 1 70.60603575493747 28.28522899215328 2 13.284784835364267 10.643939350567107 3 5.095313730116005 6.12364569553597 4 2.5551904151053457 4.094502416799729 5 1.5350072929237284 3.074668639921251 6 1.123662351667711 2.700877899767379 7 0.8385268677861627 2.351434541319967 8 0.6303197617024983 2.020080986834091 9 0.5172134385371436 1.8647910694004879 >10 3.6223632759964346 26.096777087554063 >50 0.11890329360018409 3.2331303200470765 >100 0.06424822032083552 4.4574917885305245 >500 0.004070023006591323 1.0911386467514008 >1k 0.0043607389356335605 3.962292564817607 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT 4334 0.48695972638571167 No Hit TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 3870 0.43482559785710756 No Hit CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 3697 0.415387657694503 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGC 3380 0.3797701604023317 No Hit GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 3034 0.3408942800771226 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCG 2854 0.32066983366516405 No Hit GAATCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTC 2187 0.2457270239052957 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2105 0.23651366498429233 No Hit TCCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG 1446 0.16246971950939987 No Hit TCTGTATCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 1360 0.1528069284459086 No Hit CGCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTG 1297 0.1457283722017231 No Hit CTGTCACTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT 1257 0.14123405077684345 No Hit GCTGTATCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 1142 0.12831287668031444 No Hit CCTGTATCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC 1052 0.11820065347433518 No Hit TTCCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCT 1007 0.11314454187134557 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCC 943 0.10595362759153809 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.5166222477899174 0.0 2 0.0 0.0 0.0 1.834582005635879 0.0 3 0.0 0.0 0.0 2.6333352808726174 0.0 4 0.0 0.0 0.0 3.546356678336921 0.0 5 0.0 0.0 0.0 5.124537646683415 0.0 6 0.0 0.0 0.0 5.9826159647285655 0.0 7 0.0 0.0 0.0 7.119566927187498 0.0 8 0.0 0.0 0.0 9.009092012242531 0.0 9 0.0 0.0 0.0 9.726835143795814 0.0 10 0.0 0.0 0.0 11.660741652921534 0.0 11 0.0 0.0 0.0 15.167773018790758 0.0 12 0.0 0.0 0.0 18.54772744637151 0.0 13 0.0 0.0 0.0 19.50232131701595 0.0 14 0.0 0.0 0.0 19.78849723374516 0.0 15 0.0 0.0 0.0 20.21646899142933 0.0 16 0.0 0.0 0.0 21.197804074551804 0.0 17 0.0 0.0 0.0 22.797445427702097 0.0 18 0.0 0.0 0.0 24.51832110128852 0.0 19 0.0 0.0 0.0 25.889988000161797 0.0 20 0.0 0.0 0.0 27.115140020583993 0.0 21 0.0 0.0 0.0 28.950620890504847 0.0 22 0.0 0.0 0.0 30.988009150438423 0.0 23 0.0 0.0 0.0 32.90438780600711 0.0 24 0.0 0.0 0.0 34.28672871826447 0.0 25 0.0 0.0 0.0 35.46873525300782 0.0 26 0.0 0.0 0.0 36.46018255933628 0.0 27 0.0 0.0 0.0 37.440955852280645 0.0 28 0.0 0.0 0.0 38.498244967483586 0.0 29 0.0 0.0 0.0 39.57384844249291 0.0 30 0.0 0.0 0.0 40.71338363977115 0.0 31 0.0 0.0 0.0 41.76696493979856 0.0 32 0.0 0.0 0.0 42.771108704152304 0.0 33 1.1235803562199161E-4 0.0 0.0 43.72221947569246 0.0 34 1.1235803562199161E-4 0.0 0.0 44.66917299991461 0.0 35 1.1235803562199161E-4 0.0 0.0 45.63309258751568 0.0 36 1.1235803562199161E-4 0.0 0.0 46.5666755054988 0.0 37 1.1235803562199161E-4 0.0 0.0 47.49126978063217 0.0 38 1.1235803562199161E-4 0.0 0.0 48.39889799238662 0.0 39 2.2471607124398323E-4 0.0 0.0 49.30742506842604 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCCG 30 2.1649466E-6 45.000004 1 CCTTCGC 20 7.032429E-4 45.0 36 CGGAATC 20 7.032429E-4 45.0 15 CGGCGTA 20 7.032429E-4 45.0 19 TCGTTGA 20 7.032429E-4 45.0 24 TCGTTCA 25 3.8900936E-5 45.0 16 AGATCGT 40 6.8121153E-9 45.0 23 CTCGTCC 40 6.8121153E-9 45.0 37 TTCAGCG 25 3.8900936E-5 45.0 1 GCGGTAG 25 3.8900936E-5 45.0 1 TAGACGA 20 7.032429E-4 45.0 34 GCGCGAC 50 2.1827873E-11 45.0 9 ACCGCTA 20 7.032429E-4 45.0 12 ACACCCG 25 3.8900936E-5 45.0 40 TTTCGCG 175 0.0 45.0 1 GTCTCCG 25 3.8900936E-5 45.0 1 GTTAGCG 20 7.032429E-4 45.0 1 CCTCGTC 40 6.8121153E-9 45.0 36 CCGTCGG 25 3.8900936E-5 45.0 2 GCCGATG 50 2.1827873E-11 45.0 9 >>END_MODULE