FastQCFastQC Report
Sat 14 Jan 2017
SRR2934211.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934211.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846879
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC14110.16661175917693083RNA PCR Primer, Index 41 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT13070.1543313743757963RNA PCR Primer, Index 41 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG12150.14346795705171578No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.13071524975822993No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC9760.11524668813372395No Hit
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC9590.11323931754123079Illumina PCR Primer Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA207.0322334E-445.044
TATCTCG253.8899336E-545.01
ACGTATC253.8899336E-545.030
TATCGCG253.8899336E-545.01
CGCCAAT207.0322334E-445.042
CGTTAGG2850.042.631582
GTTAGGG13700.041.5510943
CGTTTAT2300.041.086961
TCGTTAG1700.041.0294151
TCGTTTG2250.041.01
TCGTTCA556.002665E-1140.90909216
GCGATGT1650.040.909099
TTTAGGG31400.040.2707023
CCCGATC451.9272193E-840.00000441
TTAGGGA16600.039.984944
TTAGGGG33600.039.7767874
TAGGGAG10550.039.6682435
TGTAGGG12200.039.651643
TTTTAGG17150.039.6209952
TCGTAGG2450.039.4897962
Produced by FastQC (version 0.11.5)