##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934211.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	846879
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41443819010744	33.0	31.0	34.0	31.0	34.0
2	32.5882516864865	34.0	31.0	34.0	31.0	34.0
3	32.660065959836054	34.0	31.0	34.0	31.0	34.0
4	36.089559429387194	37.0	35.0	37.0	35.0	37.0
5	35.18127028772705	37.0	35.0	37.0	35.0	37.0
6	26.136477584164915	37.0	0.0	37.0	0.0	37.0
7	31.120572124234986	37.0	19.0	37.0	17.0	37.0
8	34.791841573589615	37.0	32.0	37.0	32.0	37.0
9	37.31788012218983	39.0	37.0	39.0	35.0	39.0
10	37.41892053055986	39.0	37.0	39.0	35.0	39.0
11	37.42743768590318	39.0	37.0	39.0	35.0	39.0
12	36.992328301917986	39.0	35.0	39.0	34.0	39.0
13	36.91869912939156	39.0	35.0	39.0	33.0	39.0
14	37.71502776665852	40.0	37.0	41.0	33.0	41.0
15	37.992124022440045	40.0	37.0	41.0	33.0	41.0
16	38.216819640113876	40.0	37.0	41.0	33.0	41.0
17	38.24525463495966	40.0	37.0	41.0	34.0	41.0
18	38.250595421541924	40.0	37.0	41.0	34.0	41.0
19	38.25834859525387	40.0	37.0	41.0	34.0	41.0
20	38.1511727177082	40.0	36.0	41.0	34.0	41.0
21	38.061720741688006	40.0	36.0	41.0	34.0	41.0
22	38.11902881049123	40.0	36.0	41.0	34.0	41.0
23	38.08952046278158	40.0	36.0	41.0	34.0	41.0
24	38.04427314882055	40.0	36.0	41.0	34.0	41.0
25	37.92353571171324	40.0	35.0	41.0	34.0	41.0
26	37.86653347172383	40.0	35.0	41.0	34.0	41.0
27	37.814737406406344	40.0	35.0	41.0	34.0	41.0
28	37.73578634019736	40.0	35.0	41.0	34.0	41.0
29	37.68855763338092	40.0	35.0	41.0	33.0	41.0
30	37.4576486133202	40.0	35.0	41.0	33.0	41.0
31	37.42887590789239	40.0	35.0	41.0	33.0	41.0
32	37.398933023489775	39.0	35.0	41.0	33.0	41.0
33	37.34108060301413	39.0	35.0	41.0	33.0	41.0
34	37.212909990683436	39.0	35.0	41.0	32.0	41.0
35	37.174383825788574	39.0	35.0	41.0	32.0	41.0
36	37.07504023597232	39.0	35.0	41.0	32.0	41.0
37	36.98328096457699	39.0	35.0	41.0	32.0	41.0
38	36.90748265100445	39.0	35.0	41.0	32.0	41.0
39	36.869628364854954	39.0	35.0	41.0	32.0	41.0
40	36.74166557442091	39.0	35.0	41.0	31.0	41.0
41	36.614934364885656	39.0	35.0	41.0	31.0	41.0
42	36.61297304573617	39.0	35.0	41.0	31.0	41.0
43	36.558750423614235	39.0	35.0	41.0	31.0	41.0
44	36.44323923488479	38.0	35.0	41.0	31.0	41.0
45	36.39061188198078	38.0	35.0	40.0	31.0	41.0
46	36.35248601039818	38.0	35.0	40.0	31.0	41.0
47	36.255995248435724	38.0	35.0	40.0	31.0	41.0
48	36.18140844205607	38.0	35.0	40.0	31.0	41.0
49	36.14843560886502	38.0	35.0	40.0	31.0	41.0
50	36.0417969981544	38.0	35.0	40.0	31.0	41.0
51	35.03217106576028	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	15.0
11	10.0
12	11.0
13	17.0
14	12.0
15	36.0
16	50.0
17	119.0
18	186.0
19	365.0
20	591.0
21	982.0
22	1356.0
23	2079.0
24	2963.0
25	4200.0
26	5599.0
27	6697.0
28	8143.0
29	10014.0
30	13065.0
31	17732.0
32	25714.0
33	44199.0
34	64483.0
35	69603.0
36	78205.0
37	127749.0
38	173332.0
39	189223.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.72409990093036	4.55472387436694	53.24030941846474	16.480866806237962
2	21.042911679236347	4.526266444202773	51.976964832048026	22.453857044512855
3	22.688483242588376	4.213943196135457	50.86440920131447	22.233164359961695
4	19.416233015578378	5.194012367764462	50.219688999254906	25.170065617402248
5	18.284076001412245	8.701124954096159	48.535032749660814	24.479766294830785
6	17.07800051719313	31.265859703688488	38.66089488581013	12.995244893308252
7	86.9626003242494	2.1252150543347987	7.2518033863161095	3.6603812350997016
8	88.58809818167649	2.415221064638514	5.671884649400917	3.324796104284083
9	84.03620824226365	4.31289475828306	7.294076249381552	4.356820750071734
10	46.54950707243892	25.570949332785435	14.603384899141435	13.276158695634205
11	35.387109610699994	24.533020655843394	22.383953315644856	17.695916417811752
12	32.51161027726511	21.08648342915576	27.195738706474003	19.206167587105124
13	29.203227379590235	22.158655486793272	28.873900521798273	19.764216611818217
14	21.761432270725805	23.49190380207798	33.19317163372808	21.553492293468135
15	20.296287899451986	23.71861859840662	34.497844438225535	21.48724906391586
16	27.36423975562034	22.816482638015582	29.259551836803134	20.55972576956094
17	27.484445829923754	23.311122368130512	26.67866365797239	22.525768143973345
18	27.775042243342913	22.42787930743353	28.661001158370915	21.136077290852647
19	27.465907172098962	24.661256212516783	26.284746699351384	21.588089916032867
20	26.504730900164013	29.173234901325927	26.217440744191318	18.10459345431874
21	26.848227432726517	24.90698198916256	29.739785730901346	18.505004847209577
22	24.979719652984663	21.53070273321218	31.000296382363953	22.4892812314392
23	24.024447412204104	23.677998864064406	30.814909804116052	21.482643919615434
24	23.43652398984979	26.090976396864253	28.39921641698519	22.073283196300768
25	25.913265059117062	25.108663693396576	25.664823428140267	23.313247819346092
26	22.99383973389351	25.7178416279067	25.7353175601237	25.553001078076086
27	22.15995437364724	24.42001749954834	30.54993688590696	22.870091240897462
28	20.681348811341408	25.951759342243697	27.645743961061736	25.72114788535316
29	22.184278982003335	26.656228339585702	27.737138363331717	23.42235431507925
30	26.16217901258621	23.031153210789263	28.593813283833935	22.212854492790587
31	25.578152250793796	24.61012730271975	28.674226188156748	21.137494258329703
32	27.993137154186137	24.104387994034568	25.71642466042965	22.18605019134965
33	26.94481738241236	23.35457603742683	26.178592219195423	23.52201436096538
34	22.441104337219368	25.03120280465096	29.923755341672187	22.60393751645749
35	23.52307708657317	27.147679892877257	26.72282581100724	22.606417209542332
36	26.884596264637565	26.38133664903723	25.31010923638442	21.423957849940784
37	24.047355053083145	27.853211615827057	26.487136887323924	21.612296443765874
38	25.20360051435919	26.013987830610986	26.41876820655607	22.36364344847375
39	23.313247819346092	24.693019900127407	25.72882312585387	26.264909154672626
40	25.461488595183017	24.051724036137394	27.42375238965661	23.06303497902298
41	22.625428189859473	23.60313574902672	27.43556045196539	26.335875609148413
42	23.433453893649506	22.9871091383775	29.86849361006708	23.71094335790591
43	23.13896081966845	22.662741666755227	29.561602070661806	24.636695442914515
44	24.222704778368577	23.2394474299162	27.499560149679	25.038287642036227
45	22.882843948190946	23.32422931729326	25.72008515974537	28.072841574770425
46	22.567686765169523	23.768566701972773	28.191040278481346	25.472706254376366
47	21.23243107929232	25.06225800852306	30.858953876527817	22.846357035656805
48	22.245090502893568	26.40861327297052	28.583540269625296	22.76275595451062
49	25.105475516573208	23.726057677661153	28.449046439928257	22.719420365837387
50	22.366713544674035	25.358876533719695	26.415461949109613	25.858947972496658
51	21.692827428711777	24.95008141658962	25.52383516417339	27.833255990525213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	569.0
2	1010.0
3	1937.0
4	2864.0
5	2037.0
6	1210.0
7	1178.0
8	1146.0
9	1213.5
10	1281.0
11	1299.0
12	1317.0
13	1347.5
14	1378.0
15	1374.0
16	1370.0
17	1351.0
18	1332.0
19	1445.5
20	1559.0
21	1606.5
22	1654.0
23	2018.5
24	2383.0
25	3436.0
26	5418.0
27	6347.0
28	7781.0
29	9215.0
30	10767.0
31	12319.0
32	14842.5
33	17366.0
34	19254.0
35	21142.0
36	22522.5
37	23903.0
38	26502.0
39	29101.0
40	32201.5
41	35302.0
42	39221.0
43	43140.0
44	46691.5
45	50243.0
46	55423.0
47	60603.0
48	68580.0
49	76557.0
50	77882.5
51	79208.0
52	72873.5
53	66539.0
54	62215.5
55	57892.0
56	55668.0
57	53444.0
58	50249.0
59	47054.0
60	41639.5
61	36225.0
62	32844.0
63	29463.0
64	25367.5
65	21272.0
66	18730.5
67	16189.0
68	13849.5
69	11510.0
70	9604.0
71	7698.0
72	6471.0
73	5244.0
74	4346.0
75	2696.0
76	1944.0
77	1289.0
78	634.0
79	505.5
80	377.0
81	312.5
82	248.0
83	164.5
84	81.0
85	56.5
86	32.0
87	22.0
88	12.0
89	8.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	846879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.271125159191822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9585221537502	22.18952827381208
2	9.274411294919462	5.800425527624976
3	3.5697727390739438	3.348924303403569
4	2.1641142025368136	2.706971443452532
5	1.5704694701258823	2.455517367949806
6	1.2723960731624073	2.38735541135555
7	1.1356960592814254	2.4860145528813273
8	0.9615774022711875	2.4055685837338276
9	0.8503684674035323	2.393278089804539
>10	8.092925431140777	48.439508086032085
>50	0.10861627853269139	2.229987037882451
>100	0.037935831467595105	2.1149786967229423
>500	0.0015972981670566333	0.412581308022235
>1k	0.0015972981670566333	0.6293613173220535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1411	0.16661175917693083	RNA PCR Primer, Index 41 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	1307	0.1543313743757963	RNA PCR Primer, Index 41 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1215	0.14346795705171578	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1107	0.13071524975822993	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	976	0.11524668813372395	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	959	0.11323931754123079	Illumina PCR Primer Index 3 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16118005051489057	0.0
2	0.0	0.0	0.0	0.5620637658980799	0.0
3	0.0	0.0	0.0	0.7801586767413055	0.0
4	0.0	0.0	0.0	1.036865951334252	0.0
5	0.0	0.0	0.0	1.5019855256772219	0.0
6	0.0	0.0	0.0	1.8375706564928402	0.0
7	0.0	0.0	0.0	2.262188577116684	0.0
8	0.0	0.0	0.0	2.954141028411379	0.0
9	0.0	0.0	0.0	3.307910575182523	0.0
10	0.0	0.0	0.0	3.9666823713895374	0.0
11	0.0	0.0	0.0	5.266277709094215	0.0
12	0.0	0.0	0.0	6.337150879877763	0.0
13	0.0	0.0	0.0	6.6993041508881435	0.0
14	0.0	0.0	0.0	6.8353330286853256	0.0
15	1.1808062308783191E-4	0.0	0.0	7.017531430109851	0.0
16	1.1808062308783191E-4	0.0	0.0	7.425263821632134	0.0
17	1.1808062308783191E-4	0.0	0.0	7.954619254934885	0.0
18	2.3616124617566382E-4	0.0	0.0	8.521406245756477	0.0
19	2.3616124617566382E-4	0.0	0.0	8.93728620027182	0.0
20	2.3616124617566382E-4	0.0	0.0	9.446804088895817	0.0
21	2.3616124617566382E-4	0.0	0.0	10.178431629548022	0.0
22	2.3616124617566382E-4	0.0	0.0	10.956582935696835	0.0
23	2.3616124617566382E-4	0.0	0.0	11.65998920743105	0.0
24	2.3616124617566382E-4	0.0	0.0	12.183322528956321	0.0
25	3.5424186926349575E-4	0.0	0.0	12.67406559850935	0.0
26	3.5424186926349575E-4	0.0	0.0	13.093606052340418	0.0
27	3.5424186926349575E-4	0.0	0.0	13.611271503957472	0.0
28	3.5424186926349575E-4	0.0	0.0	14.045690116297605	0.0
29	3.5424186926349575E-4	0.0	0.0	14.60539226973393	0.0
30	4.7232249235132763E-4	0.0	0.0	15.346230098986986	0.0
31	4.7232249235132763E-4	0.0	0.0	15.855157584495542	0.0
32	4.7232249235132763E-4	0.0	0.0	16.31708898201514	0.0
33	4.7232249235132763E-4	0.0	0.0	16.790001877481906	0.0
34	4.7232249235132763E-4	0.0	0.0	17.33872253297106	0.0
35	4.7232249235132763E-4	0.0	0.0	17.96242438412099	0.0
36	4.7232249235132763E-4	0.0	0.0	18.477019739537763	0.0
37	4.7232249235132763E-4	0.0	0.0	19.020426766987963	0.0
38	4.7232249235132763E-4	0.0	0.0	19.689825819272883	0.0
39	4.7232249235132763E-4	0.0	0.0	21.236681981723482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	20	7.0322334E-4	45.0	44
TATCTCG	25	3.8899336E-5	45.0	1
ACGTATC	25	3.8899336E-5	45.0	30
TATCGCG	25	3.8899336E-5	45.0	1
CGCCAAT	20	7.0322334E-4	45.0	42
CGTTAGG	285	0.0	42.63158	2
GTTAGGG	1370	0.0	41.551094	3
CGTTTAT	230	0.0	41.08696	1
TCGTTAG	170	0.0	41.029415	1
TCGTTTG	225	0.0	41.0	1
TCGTTCA	55	6.002665E-11	40.909092	16
GCGATGT	165	0.0	40.90909	9
TTTAGGG	3140	0.0	40.270702	3
CCCGATC	45	1.9272193E-8	40.000004	41
TTAGGGA	1660	0.0	39.98494	4
TTAGGGG	3360	0.0	39.776787	4
TAGGGAG	1055	0.0	39.668243	5
TGTAGGG	1220	0.0	39.65164	3
TTTTAGG	1715	0.0	39.620995	2
TCGTAGG	245	0.0	39.489796	2
>>END_MODULE