##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934210.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494132
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.430674394696155	33.0	31.0	34.0	31.0	34.0
2	32.614878210680544	34.0	31.0	34.0	31.0	34.0
3	32.65762994503493	34.0	31.0	34.0	31.0	34.0
4	36.10471291072021	37.0	35.0	37.0	35.0	37.0
5	35.18846583504003	37.0	35.0	37.0	35.0	37.0
6	25.950806667044432	37.0	0.0	37.0	0.0	37.0
7	31.04382432224588	37.0	19.0	37.0	17.0	37.0
8	34.79330421830604	37.0	32.0	37.0	32.0	37.0
9	37.34290432516008	39.0	37.0	39.0	35.0	39.0
10	37.438601426339524	39.0	37.0	39.0	35.0	39.0
11	37.462787271417355	39.0	37.0	39.0	35.0	39.0
12	37.169466863105406	39.0	37.0	39.0	34.0	39.0
13	37.13899929573474	39.0	37.0	39.0	33.0	39.0
14	38.34382917924765	40.0	38.0	41.0	34.0	41.0
15	38.482269919778524	40.0	38.0	41.0	34.0	41.0
16	38.563802789538016	40.0	38.0	41.0	35.0	41.0
17	38.57428177086285	40.0	38.0	41.0	35.0	41.0
18	38.53397877490225	40.0	38.0	41.0	35.0	41.0
19	38.52156104036978	40.0	37.0	41.0	35.0	41.0
20	38.463916524329534	40.0	37.0	41.0	34.0	41.0
21	38.35468255445913	40.0	37.0	41.0	34.0	41.0
22	38.33192750115354	40.0	37.0	41.0	34.0	41.0
23	38.283221892125994	40.0	37.0	41.0	34.0	41.0
24	38.23327167639416	40.0	37.0	41.0	34.0	41.0
25	38.09330502780634	40.0	36.0	41.0	34.0	41.0
26	38.03330891340775	40.0	36.0	41.0	34.0	41.0
27	38.01842017922336	40.0	36.0	41.0	34.0	41.0
28	37.94094897719638	40.0	36.0	41.0	34.0	41.0
29	37.920610282272754	40.0	36.0	41.0	34.0	41.0
30	37.700316919365676	40.0	36.0	41.0	33.0	41.0
31	37.71570754373325	40.0	36.0	41.0	33.0	41.0
32	37.66216314668955	40.0	35.0	41.0	33.0	41.0
33	37.56323816308193	40.0	35.0	41.0	33.0	41.0
34	37.490120048893814	40.0	35.0	41.0	33.0	41.0
35	37.41741680360713	40.0	35.0	41.0	33.0	41.0
36	37.36224733472028	40.0	35.0	41.0	33.0	41.0
37	37.305054115094755	40.0	35.0	41.0	33.0	41.0
38	37.212060744902175	39.0	35.0	41.0	33.0	41.0
39	37.16551245416205	39.0	35.0	41.0	33.0	41.0
40	37.06560595144617	39.0	35.0	41.0	32.0	41.0
41	36.991876664534985	39.0	35.0	41.0	32.0	41.0
42	36.939048675252764	39.0	35.0	41.0	32.0	41.0
43	36.85457529566998	39.0	35.0	41.0	32.0	41.0
44	36.71753499065027	39.0	35.0	41.0	31.0	41.0
45	36.66818178138635	39.0	35.0	41.0	31.0	41.0
46	36.592596715047804	39.0	35.0	41.0	31.0	41.0
47	36.51130264787547	39.0	35.0	40.0	31.0	41.0
48	36.482955971278926	39.0	35.0	40.0	31.0	41.0
49	36.4687350748383	39.0	35.0	40.0	31.0	41.0
50	36.37587527219448	38.0	35.0	40.0	31.0	41.0
51	35.42489861008799	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	3.0
11	5.0
12	6.0
13	1.0
14	8.0
15	8.0
16	15.0
17	21.0
18	76.0
19	126.0
20	234.0
21	389.0
22	638.0
23	983.0
24	1463.0
25	2134.0
26	3111.0
27	3866.0
28	4368.0
29	5137.0
30	6494.0
31	8685.0
32	12158.0
33	20866.0
34	32615.0
35	44989.0
36	45319.0
37	75829.0
38	107717.0
39	116797.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.72328041899735	4.047501477338039	54.922368921664656	18.30684918199995
2	19.61237078351534	4.031311471428687	54.93208292521027	21.424234819845708
3	20.841799357256765	3.631823075615422	53.59620506261484	21.930172504512964
4	18.681445443727586	4.5463560344199525	52.57582994017793	24.196368581674534
5	17.84705301417435	8.019314677049858	49.90731221616896	24.226320092606834
6	16.500044522516248	31.369553074886873	38.63542535193025	13.494977050666623
7	84.9787101422292	2.0925582637837663	8.792387459221422	4.13634413476561
8	86.40444253762153	2.3309561007989767	6.908882646742167	4.355718714837331
9	81.2226287712595	4.1996875328859495	9.42541669027709	5.152267005577457
10	44.238786397157035	23.547554094857244	16.605481936000906	15.608177571984813
11	32.00541555697668	25.117377542842807	24.060574907109842	18.81663199307068
12	28.751426744270763	21.488994843483116	29.318886451393556	20.44069196085257
13	24.754316660325582	23.58701723426129	30.68107307359167	20.977593031821456
14	20.901702379121367	27.071713631175477	30.794807865104872	21.231776124598287
15	18.856095132474724	24.547489334833607	36.22351922158452	20.37289631110715
16	20.596925517877814	23.065294293832416	34.04535630155505	22.29242388673472
17	21.32608290902026	23.426533800684837	29.445573247634236	25.801810042660666
18	21.923089376927624	24.291282491318107	31.777338848728682	22.00828928302559
19	24.198189957339334	25.094104409348112	29.49394089028842	21.21376474302413
20	26.99825147936179	25.303967360948086	27.7808358900051	19.916945269685023
21	25.98941173613528	25.394631394040456	28.236382181279495	20.37957468854476
22	23.98448187933589	24.116632802570972	27.53272404944428	24.366161268648863
23	22.436514939327953	24.59241660123206	27.779824014635768	25.191244444804223
24	23.210397221794988	23.911019727522202	29.521261525260456	23.357321525422357
25	22.552475856653686	24.80794605490031	28.189633539216242	24.44994454922976
26	21.12127933426696	27.50257016343811	28.081565249771316	23.294585252523618
27	21.775153197930916	26.45082690455182	30.167242761043607	21.606777136473653
28	18.96274679640258	26.242582953542776	31.925275027725387	22.869395222329256
29	20.6722090453563	24.431933167655608	31.105453603490563	23.790404183497525
30	22.61440262925696	24.02192126800126	30.49185237952612	22.87182372321566
31	23.919721855698477	25.64881448681729	27.902463309399106	22.529000348085127
32	23.487246322844907	26.487861543069464	26.162847174439218	23.86204495964641
33	23.06832991994042	25.71337213538083	26.96445484202602	24.253843102652734
34	21.16337334963127	24.91055021735083	28.986586580104102	24.939489852913795
35	20.172949738126654	24.70534189244979	28.292844826888363	26.828863542535192
36	22.872633223511126	25.512211311957127	29.01674046611027	22.59841499842147
37	21.531493608995167	25.53548444545182	30.200634648231645	22.732387297321363
38	21.198586612484114	26.23064282418463	27.067868504772	25.50290205855925
39	23.21869459982353	23.812463066549018	28.02429310386698	24.94454922976047
40	23.266657492329983	23.311382383654568	29.559308039147435	23.86265208486801
41	19.568455392486218	23.68638339552994	28.782997255793997	27.962163956189844
42	20.97415265556572	23.978613002193747	27.809977900641936	27.2372564415986
43	22.032169541741883	23.055580290286805	29.09586911999223	25.816381047979082
44	21.39752131009528	23.53541159042523	28.73705811402621	26.33000898545328
45	22.212283357483425	23.79141605886686	27.960342580525044	26.03595800312467
46	22.94103599847814	24.133632308775795	29.292577691790854	23.63275400095521
47	19.851578120826012	24.636331992261177	30.94011316814131	24.571976718771502
48	19.94203977884452	22.638282887973254	30.814640622343823	26.605036710838398
49	21.134636089142173	22.09571531493609	31.782600600649218	24.98704799527252
50	20.879238745922144	22.55429723231849	29.618806310864304	26.94765771089506
51	19.72124857325573	22.790266568447297	27.8982134328479	29.59027142544907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	93.0
1	325.0
2	557.0
3	1828.0
4	3099.0
5	2119.0
6	1139.0
7	1135.0
8	1131.0
9	1133.5
10	1136.0
11	1169.5
12	1203.0
13	1149.0
14	1095.0
15	1002.5
16	910.0
17	963.5
18	1017.0
19	1106.5
20	1196.0
21	1250.5
22	1305.0
23	1514.0
24	1723.0
25	1989.5
26	3208.5
27	4161.0
28	4887.0
29	5613.0
30	6047.5
31	6482.0
32	8240.0
33	9998.0
34	10754.5
35	11511.0
36	13132.5
37	14754.0
38	16061.5
39	17369.0
40	20558.5
41	23748.0
42	26595.0
43	29442.0
44	32940.5
45	36439.0
46	40075.0
47	43711.0
48	47999.5
49	52288.0
50	50029.5
51	47771.0
52	41804.5
53	35838.0
54	31342.0
55	26846.0
56	25398.5
57	23951.0
58	22739.0
59	21527.0
60	20466.5
61	19406.0
62	17169.0
63	14932.0
64	12390.0
65	9848.0
66	8512.5
67	7177.0
68	6051.5
69	4926.0
70	4086.5
71	3247.0
72	2768.5
73	2290.0
74	1829.5
75	1105.5
76	842.0
77	613.0
78	384.0
79	302.5
80	221.0
81	159.0
82	97.0
83	83.0
84	69.0
85	38.5
86	8.0
87	6.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494132.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.49496242423542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19776698768428	24.904592593538737
2	10.345037597643403	7.137033664160236
3	3.8573884606043753	3.991814100126817
4	1.93016813274791	2.6632430884638323
5	1.3806148453612128	2.3812128606538314
6	1.086581299581124	2.248894865995666
7	0.9174684448189644	2.2153627670616323
8	0.7964479347365044	2.19787532652765
9	0.6600021427261688	2.049007420192865
>10	6.7080642715516685	45.18411325349816
>50	0.0916670325703534	2.062630089045437
>100	0.025854804058304794	1.7064939779553387
>500	0.0	0.0
>1k	0.002938045915716454	1.257725992779769
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1470	0.2974913585843459	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	1387	0.280694227453393	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.24932609100402323	No Hit
TCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1094	0.22139833081039076	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1022	0.2068273254919738	TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.10118753693345098	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29263435681154026	0.0
2	0.0	0.0	0.0	1.0742068920855157	0.0
3	0.0	0.0	0.0	1.5261104320303076	0.0
4	0.0	0.0	0.0	2.0006799802481927	0.0
5	0.0	0.0	0.0	2.710206989225551	0.0
6	0.0	0.0	0.0	3.202990294091457	0.0
7	0.0	0.0	0.0	3.8277221471185836	0.0
8	0.0	0.0	0.0	4.871977528271798	0.0
9	0.0	0.0	0.0	5.37548671205265	0.0
10	0.0	0.0	0.0	6.276055790760363	0.0
11	0.0	0.0	0.0	8.007576922765576	0.0
12	0.0	0.0	0.0	9.53388972986975	0.0
13	0.0	0.0	0.0	10.039827414537006	0.0
14	0.0	0.0	0.0	10.203144099147597	0.0
15	0.0	0.0	0.0	10.44983931419135	0.0
16	0.0	0.0	0.0	11.080237669286749	0.0
17	0.0	0.0	0.0	11.97635449636939	0.0
18	0.0	0.0	0.0	12.987420365408433	0.0
19	0.0	0.0	0.0	13.668007738822824	0.0
20	0.0	0.0	0.0	14.381177499129787	0.0
21	0.0	0.0	0.0	15.33537597241223	0.0
22	0.0	0.0	0.0	16.32701383436005	0.0
23	0.0	0.0	0.0	17.28121230764249	0.0
24	0.0	0.0	0.0	18.031011956319364	0.0
25	0.0	0.0	0.0	18.654934309051022	0.0
26	0.0	0.0	0.0	19.23514364582743	0.0
27	0.0	0.0	0.0	19.763140213546176	0.0
28	0.0	0.0	0.0	20.31764791594149	0.0
29	0.0	0.0	0.0	20.903119004638437	0.0
30	0.0	0.0	0.0	21.528255607813296	0.0
31	0.0	0.0	0.0	22.158653962908698	0.0
32	0.0	0.0	0.0	22.733803922838433	0.0
33	0.0	0.0	0.0	23.343964770547142	0.0
34	2.0237507386690196E-4	0.0	0.0	23.902722349493658	0.0
35	2.0237507386690196E-4	0.0	0.0	24.488800563412205	0.0
36	2.0237507386690196E-4	0.0	0.0	25.0586887714214	0.0
37	2.0237507386690196E-4	0.0	0.0	25.640517108788746	0.0
38	2.0237507386690196E-4	0.0	0.0	26.21769081945715	0.0
39	2.0237507386690196E-4	0.0	0.0	26.769770020966057	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGG	20	7.0293766E-4	45.000004	27
GCAACGA	20	7.0293766E-4	45.000004	24
CGCGCAT	25	3.8875653E-5	45.000004	16
TATATCG	20	7.0293766E-4	45.000004	34
GTAATCG	25	3.8875653E-5	45.000004	37
ACGATTC	20	7.0293766E-4	45.000004	25
ATATCGG	20	7.0293766E-4	45.000004	35
AGCGCTA	20	7.0293766E-4	45.000004	24
GGACGTA	20	7.0293766E-4	45.000004	8
TTAGTGA	20	7.0293766E-4	45.000004	36
ACGTATA	20	7.0293766E-4	45.000004	36
CCGCGTA	25	3.8875653E-5	45.000004	25
AACTCGA	25	3.8875653E-5	45.000004	26
GTCCAAT	20	7.0293766E-4	45.000004	11
GGCGATA	20	7.0293766E-4	45.000004	8
GGTTCGA	20	7.0293766E-4	45.000004	9
CGCTTGT	20	7.0293766E-4	45.000004	40
TAATCGT	25	3.8875653E-5	45.000004	38
ACGAAAT	20	7.0293766E-4	45.000004	22
CATGCGG	45	3.8380676E-10	45.0	2
>>END_MODULE