FastQCFastQC Report
Sat 14 Jan 2017
SRR2934208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845831
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT37710.4458337422014563Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC34550.4084740332288601No Hit
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC32300.3818729746249546TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC31740.3752522667057604TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC31480.37217836660042014TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG29700.35113397357155274No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22860.27026675541567996No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC21910.2590351973384754No Hit
TCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG12750.1507393320887979No Hit
TCTGTATCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC12200.1442368510967321No Hit
CTGTCACTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT11360.1343057892179407Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG11170.1320594776024998No Hit
CCTGTATCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC9750.1152712539502572No Hit
GCTGTATCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC9650.11408898467897251No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.10439437665443806No Hit
TTCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT8490.10037466113207011No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA207.0322276E-445.00000413
CTATCGT351.2114106E-745.00000424
AACCCGA207.0322276E-445.00000424
CTAAGCG207.0322276E-445.00000436
CGTTACC207.0322276E-445.00000422
AGACCGC207.0322276E-445.00000425
TCGACGT207.0322276E-445.00000426
CCGTTAG302.1648193E-645.0000041
TACGTTC207.0322276E-445.00000431
TCTATCG406.8121153E-945.00000423
CGCATGA253.8899292E-545.020
GCACGGT253.8899292E-545.019
CCGTAGG900.045.02
ACGTCCA253.8899292E-545.010
GCGCTAG502.1827873E-1145.01
CGTTTAT6700.043.6567151
TAGGGTA5650.043.4070785
TCTTAGG9300.043.3064462
TAGGGCG3850.043.2467545
TTAGGGA16350.043.0733954
Produced by FastQC (version 0.11.5)