##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845831
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.225318059990705	33.0	31.0	34.0	30.0	34.0
2	32.388388460579	34.0	31.0	34.0	30.0	34.0
3	32.445510982690394	34.0	31.0	34.0	30.0	34.0
4	35.973599927172216	37.0	35.0	37.0	35.0	37.0
5	35.102046389881664	37.0	35.0	37.0	35.0	37.0
6	25.950505479226937	36.0	0.0	37.0	0.0	37.0
7	31.022071785025613	37.0	19.0	37.0	17.0	37.0
8	34.68576583265451	37.0	32.0	37.0	32.0	37.0
9	37.273950706465	39.0	37.0	39.0	35.0	39.0
10	37.2183273017896	39.0	37.0	39.0	34.0	39.0
11	37.260633625393254	39.0	37.0	39.0	34.0	39.0
12	37.1373253049368	39.0	37.0	39.0	34.0	39.0
13	37.169676921276235	39.0	37.0	39.0	34.0	39.0
14	38.35640807679076	40.0	38.0	41.0	34.0	41.0
15	38.44139313881851	40.0	38.0	41.0	34.0	41.0
16	38.535006401988106	40.0	38.0	41.0	34.0	41.0
17	38.50339252167395	40.0	38.0	41.0	34.0	41.0
18	38.49360333210772	40.0	38.0	41.0	34.0	41.0
19	38.49450776810025	40.0	38.0	41.0	34.0	41.0
20	38.41227029985895	40.0	37.0	41.0	34.0	41.0
21	38.33011677273593	40.0	37.0	41.0	34.0	41.0
22	38.3375165961049	40.0	37.0	41.0	34.0	41.0
23	38.28891114182384	40.0	37.0	41.0	34.0	41.0
24	38.239684996175356	40.0	37.0	41.0	34.0	41.0
25	38.145572815373285	40.0	37.0	41.0	34.0	41.0
26	38.108124436205344	40.0	37.0	41.0	34.0	41.0
27	38.06842146953706	40.0	37.0	41.0	34.0	41.0
28	38.053484679563645	40.0	37.0	41.0	34.0	41.0
29	38.03455536626111	40.0	37.0	41.0	34.0	41.0
30	37.78957853282748	40.0	36.0	41.0	33.0	41.0
31	37.80411926259501	40.0	36.0	41.0	33.0	41.0
32	37.72081538747102	40.0	36.0	41.0	33.0	41.0
33	37.64680060201151	40.0	36.0	41.0	33.0	41.0
34	37.580016575415186	40.0	36.0	41.0	33.0	41.0
35	37.55059225779145	40.0	36.0	41.0	33.0	41.0
36	37.48227364568099	40.0	36.0	41.0	33.0	41.0
37	37.403434019325374	40.0	36.0	41.0	33.0	41.0
38	37.32939558848044	40.0	36.0	41.0	33.0	41.0
39	37.329427509750765	40.0	36.0	41.0	33.0	41.0
40	37.24981822609954	40.0	36.0	41.0	33.0	41.0
41	37.16046349684512	39.0	36.0	41.0	32.0	41.0
42	37.15197598574656	39.0	36.0	41.0	32.0	41.0
43	37.09934963367387	39.0	35.0	41.0	32.0	41.0
44	36.99402244656439	39.0	35.0	41.0	32.0	41.0
45	36.98273413956216	39.0	35.0	41.0	32.0	41.0
46	36.8704067360974	39.0	35.0	41.0	31.0	41.0
47	36.76509137168063	39.0	35.0	41.0	31.0	41.0
48	36.771122127233454	39.0	35.0	41.0	31.0	41.0
49	36.785212412408626	39.0	35.0	41.0	31.0	41.0
50	36.70652411651973	39.0	35.0	40.0	31.0	41.0
51	35.746837134131994	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	3.0
11	2.0
12	5.0
13	3.0
14	5.0
15	8.0
16	17.0
17	46.0
18	84.0
19	178.0
20	336.0
21	574.0
22	1068.0
23	1603.0
24	2592.0
25	4049.0
26	5339.0
27	6317.0
28	6976.0
29	8462.0
30	10744.0
31	14931.0
32	20784.0
33	32751.0
34	49760.0
35	68277.0
36	83315.0
37	140258.0
38	195189.0
39	192066.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.759164655823678	4.508347412189906	55.411305568133585	14.321182363852827
2	20.036390248170143	5.865592535624729	52.39675538021189	21.70126183599324
3	19.622123095511988	5.666498390340387	52.094449127544394	22.616929386603235
4	17.63579249282658	4.216445129109716	53.938434509967124	24.20932786809658
5	17.94259136872496	7.484355621867725	51.52518647342082	23.047866535986504
6	14.632473862982085	32.01916222034898	40.62620074222865	12.722163174440285
7	81.25831283081372	1.4330285837241719	12.254575677647189	5.0540829078149185
8	82.76062239383518	2.6239284206892393	8.997542062185	5.617907123290586
9	78.4845908934527	3.4064724513525753	11.250474385545104	6.8584622696496105
10	38.87041264744376	21.179881087356694	22.200888830038153	17.7488174351614
11	25.638691417079773	26.310102136242346	26.697295322588083	21.3539111240898
12	22.743550425557824	22.314977814717125	32.158197086652066	22.783274673072988
13	22.062445098370713	25.305882617213133	33.686989481350295	18.94468280306586
14	20.347208839590888	27.069237235334242	31.727851071904432	20.85570285317043
15	17.157801026446183	26.90230081422885	35.259171158304675	20.680727001020298
16	18.705627956412098	25.01279806486166	32.51122269105767	23.770351287668575
17	18.14298601020771	26.345806668235145	30.09099926581078	25.420208055746365
18	18.982278965892714	25.03679813106874	33.36304770101829	22.61787520202026
19	20.388588264085854	27.771150501695963	30.138644717443558	21.701616516774628
20	21.949538382963027	27.27530676931917	31.083159638272896	19.69199520944491
21	20.819052505760606	28.686226917670314	30.829799333436586	19.664921243132493
22	20.02492223623868	24.85579270563505	30.099275150709776	25.020009907416497
23	18.73790390751817	26.47491047265943	29.1143266208025	25.672858999019898
24	20.120331366431355	24.870689298453236	30.309009719435682	24.699969615679727
25	18.237804005764747	26.866123374527533	29.346760759537073	25.549311860170647
26	17.871300531666492	27.621356985024192	31.235317693487236	23.27202478982208
27	17.733684388488953	27.77930815966783	30.987632281153093	23.499375170690126
28	15.709166488341051	25.95837702803515	32.745666687553424	25.586789796070374
29	16.544912636212196	25.243104118907915	32.30846351103235	25.903519733847542
30	18.422829146720797	26.90123677188469	30.02573800203587	24.650196079358643
31	18.06389219595877	28.69556684491346	26.93363094991789	26.306910009209876
32	17.06215544239925	31.153268206060076	26.403501408673836	25.38107494286684
33	17.47535855271325	27.877554736111588	25.819815069440587	28.82727164173458
34	15.916063610815872	26.96850789342079	27.66368222493619	29.45174627082715
35	15.216514882996721	27.87365324751635	28.449891290340506	28.459940579146426
36	16.689740621944573	28.166383119086436	27.68425371025654	27.45962254871245
37	15.365126130397208	29.67566807080847	27.76393865914113	27.195267139653197
38	15.157164965578229	31.407929007094797	25.497646693015508	27.937259334311467
39	16.745425504622084	26.19211166296813	28.05714143841973	29.00532139399005
40	17.881940955108053	25.561016325956366	28.078304058375725	28.47873866055985
41	15.717324146312917	27.45938609485819	26.822852319198514	30.000437439630375
42	17.92603959892697	26.63333455501158	26.997237036713006	28.44338880934844
43	18.22054287440399	25.81012046141605	28.042835980237186	27.926500683942773
44	17.52016655809494	25.33922261066336	26.838458273579473	30.302152557662225
45	18.538809762233825	24.166647947403206	26.83420210420285	30.460340186160117
46	19.41498951918291	25.557351291215387	28.027939387419	26.999719802182703
47	14.645715278820473	26.549393436750368	30.859474292145833	27.945416992283327
48	15.373520242223329	26.735955527759092	29.233499363348	28.657024866669584
49	17.687930567690234	23.671750030443434	30.225896189664365	28.414423212201967
50	17.42274757014108	23.233601038505327	27.513534027483033	31.830117363870556
51	16.556971782779303	22.828555586163194	25.381311396721095	35.23316123433641
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	349.5
2	593.0
3	2970.5
4	5348.0
5	3608.0
6	1868.0
7	1865.5
8	1863.0
9	1962.5
10	2062.0
11	2163.0
12	2264.0
13	2277.5
14	2291.0
15	2332.0
16	2373.0
17	2417.5
18	2462.0
19	2561.5
20	2661.0
21	3011.0
22	3361.0
23	3435.5
24	3510.0
25	4103.0
26	5395.5
27	6095.0
28	6871.5
29	7648.0
30	9908.5
31	12169.0
32	13962.5
33	15756.0
34	18219.5
35	20683.0
36	23431.5
37	26180.0
38	29721.5
39	33263.0
40	38846.5
41	44430.0
42	50960.0
43	57490.0
44	65265.0
45	73040.0
46	90724.0
47	108408.0
48	113214.5
49	118021.0
50	107621.0
51	97221.0
52	78418.5
53	59616.0
54	49200.5
55	38785.0
56	32265.5
57	25746.0
58	22310.5
59	18875.0
60	17044.5
61	15214.0
62	13333.5
63	11453.0
64	9484.0
65	7515.0
66	5928.5
67	4342.0
68	3655.5
69	2969.0
70	2488.5
71	2008.0
72	1661.0
73	1314.0
74	1233.0
75	833.5
76	515.0
77	366.5
78	218.0
79	139.5
80	61.0
81	87.0
82	113.0
83	77.0
84	41.0
85	27.0
86	13.0
87	13.5
88	14.0
89	8.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	845831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.366541119568176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97275541176924	29.45657731937374
2	12.582499918097136	10.158240006616625
3	4.583339081120479	5.55040636448921
4	2.1741541102219855	3.5105232516221574
5	1.3406584503785326	2.7058872232250795
6	0.8871387354507081	2.1486433345999636
7	0.6138933201638095	1.7346524965994452
8	0.49078542045295664	1.5849047884479064
9	0.41428360131503406	1.5050876424881499
>10	3.7726765131304068	30.295405951857795
>50	0.10872983196343872	2.961069365729449
>100	0.05086235150181883	3.4882432257591836
>500	0.004568474685776558	1.3383503949365685
>1k	0.0036547797486212457	3.5620086342547492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	3771	0.4458337422014563	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC	3455	0.4084740332288601	No Hit
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3230	0.3818729746249546	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3174	0.3752522667057604	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3148	0.37217836660042014	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG	2970	0.35113397357155274	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2286	0.27026675541567996	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	2191	0.2590351973384754	No Hit
TCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	1275	0.1507393320887979	No Hit
TCTGTATCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1220	0.1442368510967321	No Hit
CTGTCACTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	1136	0.1343057892179407	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	1117	0.1320594776024998	No Hit
CCTGTATCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	975	0.1152712539502572	No Hit
GCTGTATCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	965	0.11408898467897251	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	883	0.10439437665443806	No Hit
TTCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	849	0.10037466113207011	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.546807813854068E-4	0.0	0.0	0.46806040450160846	0.0
2	3.546807813854068E-4	0.0	0.0	1.683669669236526	0.0
3	3.546807813854068E-4	0.0	0.0	2.4094647748781965	0.0
4	3.546807813854068E-4	0.0	0.0	3.2601075155675305	0.0
5	3.546807813854068E-4	0.0	0.0	4.766318567184225	0.0
6	3.546807813854068E-4	0.0	0.0	5.636114070068371	0.0
7	3.546807813854068E-4	0.0	0.0	6.7087869799049695	0.0
8	3.546807813854068E-4	0.0	0.0	8.425914869518852	0.0
9	3.546807813854068E-4	0.0	0.0	9.05925651814606	0.0
10	3.546807813854068E-4	0.0	0.0	10.939655794124358	0.0
11	3.546807813854068E-4	0.0	0.0	14.031999300096592	0.0
12	3.546807813854068E-4	0.0	0.0	16.672479490583818	0.0
13	3.546807813854068E-4	0.0	0.0	17.562137117225546	0.0
14	3.546807813854068E-4	0.0	0.0	17.838788126706163	0.0
15	3.546807813854068E-4	0.0	0.0	18.292543073025225	0.0
16	3.546807813854068E-4	0.0	0.0	19.383659383493868	0.0
17	3.546807813854068E-4	0.0	0.0	21.02275750120296	0.0
18	3.546807813854068E-4	0.0	0.0	22.73231886748062	0.0
19	3.546807813854068E-4	0.0	0.0	23.98469670655249	0.0
20	4.729077085138757E-4	0.0	0.0	25.115182583754912	0.0
21	4.729077085138757E-4	0.0	0.0	26.947108819610538	0.0
22	4.729077085138757E-4	0.0	0.0	28.845951496220877	0.0
23	4.729077085138757E-4	0.0	0.0	30.95606569160979	0.0
24	4.729077085138757E-4	0.0	0.0	32.38767555220842	0.0
25	4.729077085138757E-4	0.0	0.0	33.62834892549457	0.0
26	4.729077085138757E-4	0.0	0.0	34.65846014156492	0.0
27	4.729077085138757E-4	0.0	0.0	35.67982256502776	0.0
28	4.729077085138757E-4	0.0	0.0	36.77046596778789	0.0
29	4.729077085138757E-4	0.0	0.0	37.909818864524944	0.0
30	5.911346356423446E-4	0.0	0.0	39.045152045739634	0.0
31	5.911346356423446E-4	0.0	0.0	40.15683984152863	0.0
32	5.911346356423446E-4	0.0	0.0	41.16957169931109	0.0
33	5.911346356423446E-4	0.0	0.0	42.205594261737865	0.0
34	5.911346356423446E-4	0.0	0.0	43.12670025099577	0.0
35	5.911346356423446E-4	0.0	0.0	44.07925460286984	0.0
36	5.911346356423446E-4	0.0	0.0	44.98546399930955	0.0
37	5.911346356423446E-4	0.0	0.0	45.88942708413383	0.0
38	5.911346356423446E-4	0.0	0.0	46.78996158807138	0.0
39	5.911346356423446E-4	0.0	0.0	47.66791474892739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	20	7.0322276E-4	45.000004	13
CTATCGT	35	1.2114106E-7	45.000004	24
AACCCGA	20	7.0322276E-4	45.000004	24
CTAAGCG	20	7.0322276E-4	45.000004	36
CGTTACC	20	7.0322276E-4	45.000004	22
AGACCGC	20	7.0322276E-4	45.000004	25
TCGACGT	20	7.0322276E-4	45.000004	26
CCGTTAG	30	2.1648193E-6	45.000004	1
TACGTTC	20	7.0322276E-4	45.000004	31
TCTATCG	40	6.8121153E-9	45.000004	23
CGCATGA	25	3.8899292E-5	45.0	20
GCACGGT	25	3.8899292E-5	45.0	19
CCGTAGG	90	0.0	45.0	2
ACGTCCA	25	3.8899292E-5	45.0	10
GCGCTAG	50	2.1827873E-11	45.0	1
CGTTTAT	670	0.0	43.656715	1
TAGGGTA	565	0.0	43.407078	5
TCTTAGG	930	0.0	43.306446	2
TAGGGCG	385	0.0	43.246754	5
TTAGGGA	1635	0.0	43.073395	4
>>END_MODULE