##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934207.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1136548
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29789326979591	33.0	31.0	34.0	31.0	34.0
2	32.46414757669716	34.0	31.0	34.0	31.0	34.0
3	32.543423594955954	34.0	31.0	34.0	31.0	34.0
4	36.023832693383824	37.0	35.0	37.0	35.0	37.0
5	35.141332350239495	37.0	35.0	37.0	35.0	37.0
6	26.159859504394007	36.0	0.0	37.0	0.0	37.0
7	31.126728479571476	37.0	19.0	37.0	17.0	37.0
8	34.70746330115402	37.0	32.0	37.0	32.0	37.0
9	37.24939025892439	39.0	37.0	39.0	35.0	39.0
10	37.31561623442213	39.0	37.0	39.0	34.0	39.0
11	37.36074147330337	39.0	37.0	39.0	35.0	39.0
12	36.90438855200132	39.0	35.0	39.0	33.0	39.0
13	36.78252832260494	39.0	35.0	39.0	33.0	39.0
14	37.410975163389494	40.0	36.0	41.0	31.0	41.0
15	37.69184495507449	40.0	36.0	41.0	32.0	41.0
16	37.966971038618695	40.0	36.0	41.0	33.0	41.0
17	38.000977521406924	40.0	36.0	41.0	33.0	41.0
18	38.04538831619958	40.0	36.0	41.0	33.0	41.0
19	38.06006697473402	40.0	36.0	41.0	34.0	41.0
20	37.90658643541672	40.0	36.0	41.0	33.0	41.0
21	37.83616002139813	40.0	36.0	41.0	33.0	41.0
22	37.9389766204331	40.0	36.0	41.0	34.0	41.0
23	37.92573916807737	40.0	35.0	41.0	34.0	41.0
24	37.84087429655413	40.0	35.0	41.0	34.0	41.0
25	37.72316083438623	39.0	35.0	41.0	33.0	41.0
26	37.706684627486034	39.0	35.0	41.0	34.0	41.0
27	37.68180314425788	39.0	35.0	41.0	34.0	41.0
28	37.6093381009865	39.0	35.0	41.0	33.0	41.0
29	37.555232159134505	39.0	35.0	41.0	33.0	41.0
30	37.29478297441023	39.0	35.0	41.0	33.0	41.0
31	37.27257713708528	39.0	35.0	41.0	32.0	41.0
32	37.21454175274604	39.0	35.0	41.0	32.0	41.0
33	37.146153088122986	39.0	35.0	41.0	32.0	41.0
34	37.014653142674135	39.0	35.0	41.0	32.0	41.0
35	36.99535699328141	39.0	35.0	41.0	32.0	41.0
36	36.92523764944375	39.0	35.0	41.0	31.0	41.0
37	36.86595990666474	39.0	35.0	41.0	31.0	41.0
38	36.72412867736338	39.0	35.0	41.0	31.0	41.0
39	36.670182869531246	39.0	35.0	41.0	31.0	41.0
40	36.52754833055885	39.0	35.0	41.0	31.0	41.0
41	36.380671999774755	39.0	35.0	41.0	31.0	41.0
42	36.40349813646234	39.0	35.0	41.0	31.0	41.0
43	36.395938402953504	38.0	35.0	40.0	31.0	41.0
44	36.31499417534499	38.0	35.0	40.0	31.0	41.0
45	36.263313120079395	38.0	35.0	40.0	31.0	41.0
46	36.232320148379124	38.0	35.0	40.0	31.0	41.0
47	36.095246307239115	38.0	35.0	40.0	31.0	41.0
48	36.056431404568926	38.0	35.0	40.0	31.0	41.0
49	36.03604423218377	38.0	35.0	40.0	31.0	41.0
50	35.93630977310241	38.0	35.0	40.0	30.0	41.0
51	34.89571579906876	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	3.0
10	8.0
11	4.0
12	13.0
13	7.0
14	9.0
15	44.0
16	72.0
17	142.0
18	272.0
19	477.0
20	851.0
21	1395.0
22	2066.0
23	2986.0
24	4289.0
25	5901.0
26	8022.0
27	9821.0
28	11885.0
29	14516.0
30	19670.0
31	26284.0
32	38694.0
33	64073.0
34	90961.0
35	91698.0
36	106060.0
37	173216.0
38	232193.0
39	230791.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.034363704832526	4.329337608266435	51.17883274617526	16.457465940725776
2	20.34062793652358	5.964552311033057	51.188071247320835	22.506748505122527
3	21.914428603103435	6.412311666555218	49.411991398515504	22.261268331825846
4	19.540661723042057	4.688759295691867	51.3364151800012	24.434163801264884
5	20.1079936791055	8.068730929094063	48.28049497249566	23.54278041930477
6	16.7628643928809	31.600337161298953	38.333180824742996	13.303617621077157
7	83.64230987164643	1.9355979685855766	10.079028778370997	4.343063381397002
8	85.05192917501064	3.4282757965347703	7.2701724872156746	4.249622541238909
9	80.53922931543586	3.9700039065661987	9.111361772666003	6.379405005331935
10	45.028014654902385	23.124056353097274	17.66788556224638	14.180043429753958
11	36.139784681333296	23.795299450617133	22.1364165877728	17.928499280276768
12	32.102471694992204	20.728820956088086	27.357137577999346	19.811569770920364
13	29.363388083917265	21.779458500652854	29.705476583479097	19.151676831950784
14	21.50793455269817	23.187933989589528	34.55463385620317	20.74949760150913
15	19.71953670236541	22.29109549266727	36.817538722517654	21.171829082449666
16	27.33593301822712	22.91280262690181	28.165814378275268	21.585449976595797
17	27.05094725431746	22.60960381787659	27.626549868549326	22.712899059256625
18	28.445960927299158	20.62411794310491	29.70644442645625	21.22347670313968
19	27.184685556615296	23.41669687509898	26.72997532880266	22.668642239483066
20	24.757775298535563	30.23761424946417	26.97756715950404	18.027043292496224
21	24.491794451268227	26.283535759158433	31.276813649753464	17.947856139819876
22	22.764194737045862	21.316829557572582	33.53267965805228	22.38629604732928
23	22.445774397561742	24.03004536543991	32.12086071155816	21.403319525440192
24	22.557340297110194	26.500684528942024	28.532186937991185	22.409788235956597
25	24.669525616164034	26.266378542745223	25.581233700644407	23.482862140446333
26	21.345424918261262	24.394130296300727	25.949190003413847	28.311254782024164
27	21.1601269809986	23.66974382076252	30.421064486497713	24.749064711741166
28	18.675938015816314	25.057982592904125	28.266118104998643	27.99996128628091
29	21.94636742134956	25.91355578470949	26.737542101169502	25.402534692771443
30	25.697374857903053	22.7785364102528	29.003350496415464	22.520738235428684
31	26.176545117320167	23.238701753027588	28.418421395312826	22.16633173433942
32	27.875989399479828	24.098322288191966	25.106814670387877	22.918873641940333
33	26.217722436711867	24.402224983018755	24.766485885329963	24.61356669493941
34	21.678538873853107	23.840788070543432	31.11791143004959	23.362761625553873
35	20.55795267775756	28.424756367526932	26.082224419910116	24.93506653480539
36	25.133738302297836	27.222783375625138	23.560729507244744	24.082748814832282
37	23.087542277140958	27.49668293816011	25.556157768963562	23.859617015735367
38	25.75139809317336	26.978183059580413	24.762526527696146	22.507892319550077
39	23.129247510883836	24.53596328531659	26.03981530036567	26.294973903433906
40	24.494346037298907	24.699704719906244	28.368269531951135	22.437679710843714
41	20.43670834843755	25.298183622689052	26.23197612419361	28.033131904679788
42	20.573438165392048	25.139985288786747	29.488591770871093	24.797984774950113
43	21.705374520037868	23.13998176935774	28.77186005342493	26.38278365717946
44	24.094186959107756	22.461699813822207	27.173775326690997	26.270337900379044
45	22.451053541073495	20.920013936938872	25.587128744232533	31.041803777755096
46	22.65359668047456	22.70524430116458	27.70784867863038	26.933310339730482
47	18.54237568496887	24.91447787510954	31.955799491090563	24.58734694883102
48	20.85235291426319	27.950513308720794	27.551322073506796	23.645811703509224
49	24.76173465616938	23.27873525799174	28.93454565931223	23.02498442652664
50	21.6808265027082	24.811622562355485	26.59210169742061	26.915449237515706
51	20.0971714349064	24.8524479388464	24.877083941901265	30.173296684345928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	650.5
2	1131.0
3	2962.5
4	4794.0
5	3219.0
6	1644.0
7	1648.0
8	1652.0
9	1724.0
10	1796.0
11	1816.5
12	1837.0
13	1762.5
14	1688.0
15	1746.5
16	1805.0
17	1718.0
18	1631.0
19	1751.0
20	1871.0
21	2260.5
22	2650.0
23	3183.5
24	3717.0
25	4410.5
26	6370.5
27	7637.0
28	8870.0
29	10103.0
30	11834.5
31	13566.0
32	15746.5
33	17927.0
34	21490.5
35	25054.0
36	27766.0
37	30478.0
38	31455.5
39	32433.0
40	36586.0
41	40739.0
42	46040.5
43	51342.0
44	58532.5
45	65723.0
46	84480.5
47	103238.0
48	110776.0
49	118314.0
50	119482.5
51	120651.0
52	111179.0
53	101707.0
54	95096.5
55	88486.0
56	81264.5
57	74043.0
58	67635.5
59	61228.0
60	51143.0
61	41058.0
62	35333.5
63	29609.0
64	26184.0
65	22759.0
66	19832.0
67	16905.0
68	14558.0
69	12211.0
70	9916.5
71	7622.0
72	6323.5
73	5025.0
74	4204.5
75	2677.0
76	1970.0
77	1419.0
78	868.0
79	697.5
80	527.0
81	369.0
82	211.0
83	160.0
84	109.0
85	96.0
86	83.0
87	54.5
88	26.0
89	21.5
90	17.0
91	11.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1136548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.82869907109267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.24527340429829	22.358156683520395
2	10.812973332067315	6.8832574850024155
3	4.123805998826837	3.9376614049267835
4	2.3574341127821916	3.001362438226908
5	1.6141271382376186	2.568778347272458
6	1.1904748109452798	2.273475870557595
7	1.0685408964933156	2.380718664774893
8	0.8267279534269633	2.10509401946297
9	0.734262464221275	2.1033557111608117
>10	6.813335597176218	41.802148261616516
>50	0.13515810600447128	2.932758461249466
>100	0.06929579741841613	3.867082617088349
>500	0.004867886591026849	1.082564395488831
>1k	0.003722501510785238	2.703585639651514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	4677	0.4115092367414311	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	3870	0.3405047565083041	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC	3172	0.27909072032153504	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	2703	0.23782541520463718	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	2483	0.21846855566152948	No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	2418	0.2127494835237931	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1995	0.1755315217659087	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1789	0.15740646237554418	No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1753	0.1542389762684902	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1565	0.1376976599316527	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7597145039188842E-4	0.0	0.0	0.2504953596328532	0.0
2	1.7597145039188842E-4	0.0	0.0	0.8877759672270771	0.0
3	1.7597145039188842E-4	0.0	0.0	1.2439421828202593	0.0
4	2.639571755878326E-4	0.0	0.0	1.7209127991074729	0.0
5	2.639571755878326E-4	0.0	0.0	2.692275205270697	0.0
6	2.639571755878326E-4	0.0	0.0	3.2098072408732405	0.0
7	2.639571755878326E-4	0.0	0.0	3.956982019237199	0.0
8	2.639571755878326E-4	0.0	0.0	5.097101046326244	0.0
9	2.639571755878326E-4	0.0	0.0	5.594220393683329	0.0
10	2.639571755878326E-4	0.0	0.0	6.880571696048033	0.0
11	2.639571755878326E-4	0.0	0.0	8.634127199203201	0.0
12	2.639571755878326E-4	0.0	0.0	10.591985556263351	0.0
13	2.639571755878326E-4	0.0	0.0	11.140928495760848	0.0
14	3.5194290078377683E-4	0.0	0.0	11.384209025927634	0.0
15	4.3992862597972104E-4	0.0	0.0	11.747590070986883	0.0
16	4.3992862597972104E-4	0.0	0.0	12.346156959494891	0.0
17	4.3992862597972104E-4	0.0	0.0	13.1139203975547	0.0
18	5.279143511756652E-4	0.0	0.0	13.913622653860637	0.0
19	5.279143511756652E-4	0.0	0.0	14.59603993848038	0.0
20	5.279143511756652E-4	0.0	0.0	15.305116897834495	0.0
21	5.279143511756652E-4	0.0	0.0	16.246828114606686	0.0
22	5.279143511756652E-4	0.0	0.0	17.284883700468438	0.0
23	5.279143511756652E-4	0.0	0.0	18.201606971284978	0.0
24	5.279143511756652E-4	0.0	0.0	18.89467052865343	0.0
25	6.159000763716095E-4	0.0	0.0	19.539077979988527	0.0
26	6.159000763716095E-4	0.0	0.0	20.111425122388145	0.0
27	6.159000763716095E-4	0.0	0.0	20.81372718090217	0.0
28	6.159000763716095E-4	0.0	0.0	21.412821983761354	0.0
29	6.159000763716095E-4	0.0	0.0	22.164747991285893	0.0
30	6.159000763716095E-4	0.0	0.0	23.151947827984387	0.0
31	6.159000763716095E-4	0.0	0.0	23.77532669099765	0.0
32	6.159000763716095E-4	0.0	0.0	24.37565329400958	0.0
33	6.159000763716095E-4	0.0	0.0	25.004663243435385	0.0
34	6.159000763716095E-4	0.0	0.0	25.7007183154605	0.0
35	6.159000763716095E-4	0.0	0.0	26.507987344133287	0.0
36	6.159000763716095E-4	0.0	0.0	27.136909307833896	0.0
37	6.159000763716095E-4	0.0	0.0	27.820294435430796	0.0
38	6.159000763716095E-4	0.0	0.0	28.714757317772765	0.0
39	7.038858015675537E-4	0.0	0.0	30.763944857586306	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.033252E-4	45.000004	16
CGACATA	20	7.033252E-4	45.000004	15
ACGGGTA	55	1.8189894E-12	45.000004	5
TTATCCG	20	7.033252E-4	45.000004	1
TCGAAAC	25	3.8907772E-5	45.000004	33
TCGTTAG	140	0.0	45.0	1
CGTTAGG	360	0.0	44.375004	2
TTAGGGA	2045	0.0	41.809296	4
TTTAGGG	3940	0.0	41.744926	3
TTAGGGT	800	0.0	41.343754	4
CACGACC	895	0.0	41.22905	27
CGACCAA	885	0.0	41.18644	29
GCGTTAG	170	0.0	41.02941	1
CGTTTAT	390	0.0	40.961536	1
ACGACCA	900	0.0	40.75	28
ACACGTG	845	0.0	40.739647	42
TGTAGGG	1480	0.0	40.591217	3
TAGGGAG	1375	0.0	40.58182	5
ACACGAC	910	0.0	40.54945	26
CCGATGA	100	0.0	40.500004	18
>>END_MODULE