SRR2934206.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934206.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	274750
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	13076	4.759235668789809	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	10961	3.989444949954504	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	8314	3.026023657870792	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	2995	1.0900818926296634	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC	2734	0.9950864422202002	No Hit
GAATCAGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	2656	0.9666969972702456	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA	2324	0.845859872611465	No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2322	0.8451319381255687	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCT	2129	0.7748862602365787	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	1817	0.6613284804367607	TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	1407	0.5121019108280255	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1225	0.445859872611465	TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.39963603275705184	No Hit
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	940	0.3421292083712466	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGT	901	0.32793448589626933	No Hit
GCTGTATCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	705	0.25659690627843496	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTAT	698	0.254049135577798	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG	672	0.24458598726114653	No Hit
GAACTATCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	608	0.2212920837124659	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.1510464058234759	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTCTCTC	378	0.1375796178343949	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACCCTCTCTCTCT	367	0.13357597816196543	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT	342	0.12447679708826205	No Hit
CTGTCACTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	342	0.12447679708826205	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	322	0.11719745222929936	No Hit
CCTGTATCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	308	0.11210191082802548	TruSeq Adapter, Index 27 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	308	0.11210191082802548	TruSeq Adapter, Index 27 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	302	0.10991810737033667	TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCG	285	0.10373066424021837	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACT	35	1.207427E-7	45.000004	7
GACCGAT	70	0.0	45.000004	9
GTGTGCA	35	1.207427E-7	45.000004	17
CGGGGTA	45	3.8198777E-10	45.000004	6
CTTATGG	35	1.207427E-7	45.000004	2
TAGGGCG	35	1.207427E-7	45.000004	5
CATACCA	35	1.207427E-7	45.000004	10
CGTAAGG	35	1.207427E-7	45.000004	2
CCTTCGA	20	7.023907E-4	45.0	11
CTTCGTT	20	7.023907E-4	45.0	31
TCGCCCT	20	7.023907E-4	45.0	16
TCGCAGG	40	6.7830115E-9	45.0	2
ACCGGCG	50	2.1827873E-11	45.0	20
TCCGCAA	20	7.023907E-4	45.0	32
ATAGGGT	25	3.8830334E-5	45.0	4
CGCGGGC	25	3.8830334E-5	45.0	4
CCGATGA	195	0.0	45.0	18
TTAGCGG	40	6.7830115E-9	45.0	2
CCCTAAG	20	7.023907E-4	45.0	19
GTCCTAA	20	7.023907E-4	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content