SRR2934203.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934203.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721139
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	3907	0.541781820148404	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	3860	0.5352643526421398	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3627	0.5029543541536374	No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3483	0.48298594307061465	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	3135	0.43472894961997616	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3125	0.43334225440587737	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	2421	0.3357189113333213	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1845	0.25584526700123	No Hit
TCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1252	0.1736142408051707	No Hit
TCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1226	0.17000883324851382	No Hit
GCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1155	0.16016329722841227	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1134	0.15725123727880477	No Hit
CTGTCACTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	1076	0.1492084050370317	No Hit
CCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	947	0.13132003677515708	No Hit
GAATCAGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	881	0.12216784836210494	No Hit
TGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	801	0.11107428664931449	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	790	0.1095489219138058	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	786	0.10899424382816628	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	776	0.10760754861406747	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	738	0.10233810680049199	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGAT	30	2.1643718E-6	45.000004	45
TAGCGCG	30	2.1643718E-6	45.000004	1
TGCGGCC	25	3.889354E-5	45.000004	44
GCGTTAG	75	0.0	45.000004	1
ATCCGGG	25	3.889354E-5	45.000004	3
CCCGTAG	25	3.889354E-5	45.000004	34
ATAAGCG	25	3.889354E-5	45.000004	33
CGGGACG	30	2.1643718E-6	45.000004	6
TCCGTAG	25	3.889354E-5	45.000004	1
CAAGCGT	25	3.889354E-5	45.000004	17
TTATTCG	25	3.889354E-5	45.000004	1
CGGAGTT	25	3.889354E-5	45.000004	14
ATCGCTT	30	2.1643718E-6	45.000004	23
AACGTCA	40	6.8084773E-9	45.0	12
CGGGTAT	40	6.8084773E-9	45.0	6
AGCCGGT	20	7.0315367E-4	45.0	16
GCGAATC	20	7.0315367E-4	45.0	18
AACCCGA	20	7.0315367E-4	45.0	24
TTTAACG	20	7.0315367E-4	45.0	24
AACTTCG	20	7.0315367E-4	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content