##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934203.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721139
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18588094666909	33.0	31.0	34.0	30.0	34.0
2	32.33384409940386	34.0	31.0	34.0	30.0	34.0
3	32.40600771834556	34.0	31.0	34.0	30.0	34.0
4	35.931298959007904	37.0	35.0	37.0	35.0	37.0
5	35.02916636043814	37.0	35.0	37.0	35.0	37.0
6	25.77900793050993	35.0	0.0	37.0	0.0	37.0
7	30.94575109652924	37.0	19.0	37.0	17.0	37.0
8	34.65193672787077	37.0	32.0	37.0	32.0	37.0
9	37.22512996800894	39.0	37.0	39.0	34.0	39.0
10	37.225037059429596	39.0	37.0	39.0	34.0	39.0
11	37.21171646520296	39.0	37.0	39.0	34.0	39.0
12	37.08679741353609	39.0	37.0	39.0	34.0	39.0
13	37.134354125903606	39.0	37.0	39.0	33.0	39.0
14	38.22095462871929	40.0	38.0	41.0	33.0	41.0
15	38.338934657534814	40.0	38.0	41.0	34.0	41.0
16	38.43787258767034	40.0	38.0	41.0	34.0	41.0
17	38.43148824290463	40.0	38.0	41.0	34.0	41.0
18	38.430236057126294	40.0	38.0	41.0	34.0	41.0
19	38.45195863765515	40.0	38.0	41.0	34.0	41.0
20	38.3733676863961	40.0	37.0	41.0	34.0	41.0
21	38.298061816099256	40.0	37.0	41.0	34.0	41.0
22	38.319292119827104	40.0	37.0	41.0	34.0	41.0
23	38.2467346794446	40.0	37.0	41.0	34.0	41.0
24	38.1911143344071	40.0	37.0	41.0	34.0	41.0
25	38.084483019223754	40.0	37.0	41.0	34.0	41.0
26	38.06060274094176	40.0	37.0	41.0	34.0	41.0
27	38.022377100669914	40.0	37.0	41.0	34.0	41.0
28	37.913075010504215	40.0	37.0	41.0	34.0	41.0
29	37.91422596753192	40.0	37.0	41.0	34.0	41.0
30	37.702929670978826	40.0	36.0	41.0	33.0	41.0
31	37.6801975763341	40.0	36.0	41.0	33.0	41.0
32	37.61635551537221	40.0	36.0	41.0	33.0	41.0
33	37.59063093245546	40.0	36.0	41.0	33.0	41.0
34	37.50212927050125	40.0	36.0	41.0	33.0	41.0
35	37.48887662434011	40.0	36.0	41.0	33.0	41.0
36	37.42383923210366	40.0	36.0	41.0	33.0	41.0
37	37.22595505166133	39.0	36.0	41.0	32.0	41.0
38	37.07745802126913	39.0	35.0	41.0	32.0	41.0
39	37.12103353167697	39.0	35.0	41.0	32.0	41.0
40	37.14671928712772	39.0	36.0	41.0	32.0	41.0
41	37.095385216997	39.0	35.0	41.0	32.0	41.0
42	37.01721998116868	39.0	35.0	41.0	32.0	41.0
43	36.88585279675624	39.0	35.0	41.0	31.0	41.0
44	36.71314961470674	39.0	35.0	40.0	31.0	41.0
45	36.738817343119706	39.0	35.0	40.0	31.0	41.0
46	36.68425643322577	39.0	35.0	40.0	31.0	41.0
47	36.547080382561475	39.0	35.0	40.0	31.0	41.0
48	36.51063109885889	39.0	35.0	40.0	31.0	41.0
49	36.46786264506565	39.0	35.0	40.0	31.0	41.0
50	36.33040786866332	39.0	35.0	40.0	31.0	41.0
51	35.26304498855283	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	3.0
12	1.0
13	6.0
14	1.0
15	14.0
16	24.0
17	29.0
18	88.0
19	185.0
20	315.0
21	612.0
22	971.0
23	1453.0
24	2232.0
25	3205.0
26	4134.0
27	4972.0
28	6006.0
29	7716.0
30	10089.0
31	14077.0
32	19674.0
33	30159.0
34	44098.0
35	60190.0
36	76049.0
37	124780.0
38	164133.0
39	145851.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.25579534597352	4.029042944564085	58.13650350348546	13.578658205976934
2	19.11600953491629	6.2101758468201	53.711975083860395	20.961839534403214
3	18.445819737942337	5.887214531456488	54.042424553380144	21.624541177221033
4	17.92955310973335	3.702198882600997	56.077677119112955	22.29057088855269
5	18.112180869430166	7.405923129937501	51.73454770855549	22.74734829207684
6	14.985321831158766	31.867781384726108	39.9346034537031	13.212293330412027
7	81.80226003585994	0.9763721002469704	11.953451415053133	5.26791644883996
8	82.69168634618292	2.583135844823259	8.479779903735619	6.24539790525821
9	77.83561837593031	4.07078247050846	10.743559840751923	7.35003931280932
10	38.68532973532148	33.39702886683427	15.984158393874134	11.933483003970109
11	22.75580713288284	24.452706066375555	31.631904528807897	21.159582271933704
12	21.247360078986162	22.144829221550907	34.90824931115915	21.69956138830378
13	22.26879977369134	24.28144920743435	35.708649788736984	17.741101230137325
14	18.8270222522981	28.202191255777322	31.82867657968852	21.14210991223606
15	15.056320626120623	26.613593218505727	36.71691587890823	21.613170276465425
16	17.271704900164877	27.900446377189418	31.70290332376976	23.124945398875944
17	18.146293571696994	27.15787109003951	30.090315459294253	24.605519878969243
18	18.737719080510136	25.533357646722756	33.344195779177106	22.384727493590002
19	18.84047319587486	27.829447582227562	31.135467642160524	22.194611579737057
20	20.76756353490797	30.17684524065402	30.511870804380294	18.543720420057713
21	20.58174637621873	29.676664276928584	30.83746684065069	18.904122506201997
22	19.16468253693116	26.395050052763754	30.425341023020525	24.01492638728456
23	18.056713060866215	28.8026302834821	28.137293919757493	25.003362735894193
24	20.2725133434747	27.182831603893288	28.411443563584832	24.133211489047188
25	18.236151421570597	29.649346381210833	26.660047508178035	25.454454689040535
26	19.94650129864007	28.36277056156996	27.06912259633718	24.621605543452787
27	21.371330631126593	27.956052855274784	29.18785421395875	21.484762299639875
28	17.276835672457043	28.25696571673422	30.783663066343657	23.682535544465075
29	20.343512138436555	26.093305174175853	29.173571253253535	24.389611434134057
30	22.768287389809732	27.332594687015956	28.101794522276563	21.797323400897746
31	21.439417366138844	31.45953831369542	25.153403158059678	21.94764116210606
32	21.08192733994417	33.826488374640675	23.314367965121843	21.777216320293313
33	22.807808203411547	27.89961436006096	23.960151926327658	25.332425510199837
34	20.72624001752783	28.07530864368728	27.20155198928362	23.996899349501273
35	21.38949633843129	28.73579157416254	24.668337172167917	25.206374915238257
36	27.334258721272874	26.381599109186993	24.53840383060686	21.745738338933272
37	20.848823874454162	30.732771351986234	29.218916186754562	19.199488586805042
38	18.17763288353563	33.914820859778764	25.16200066838709	22.745545588298512
39	19.60662230166445	29.050571387762968	27.90779586182414	23.43501044874844
40	20.659678647251084	25.070756123299393	30.038591727808374	24.230973501641152
41	21.115485364125362	27.10795006233195	27.192538470391973	24.58402610315071
42	24.431766968642663	26.577400473417747	26.267196754023843	22.72363580391575
43	25.748295404908067	24.963287244206732	26.40919434394756	22.879223006937636
44	20.992901507199026	25.86450046384955	26.892873634625225	26.249724394326197
45	21.590983153039844	24.580975373679692	25.386090614985463	28.441950858295
46	24.82655909609659	25.025827198362588	27.846781272403792	22.300832433137025
47	19.09396108101212	26.88732685376883	31.03839897717361	22.98031308804544
48	19.900740356574808	27.3626859731619	28.62222123612785	24.114352434135444
49	21.93266485379379	23.773780089552776	30.99263803510835	23.300917021545082
50	22.26186629762085	22.710878207946042	28.205241985248335	26.822013509184778
51	19.55018380645063	22.59550516613302	26.009410113722875	31.844900913693476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	229.0
2	368.0
3	1825.0
4	3282.0
5	2061.0
6	840.0
7	999.0
8	1158.0
9	1553.5
10	1949.0
11	2223.0
12	2497.0
13	2585.5
14	2674.0
15	2657.0
16	2640.0
17	2551.0
18	2462.0
19	2323.5
20	2185.0
21	2222.5
22	2260.0
23	2468.5
24	2677.0
25	2915.0
26	3787.5
27	4422.0
28	5978.0
29	7534.0
30	8142.0
31	8750.0
32	10972.0
33	13194.0
34	15527.0
35	17860.0
36	20280.5
37	22701.0
38	25319.0
39	27937.0
40	33481.0
41	39025.0
42	44692.5
43	50360.0
44	56257.0
45	62154.0
46	81642.0
47	101130.0
48	107106.5
49	113083.0
50	101141.5
51	89200.0
52	70870.0
53	52540.0
54	41644.0
55	30748.0
56	25435.5
57	20123.0
58	16531.0
59	12939.0
60	10500.5
61	8062.0
62	6293.5
63	4525.0
64	3714.0
65	2903.0
66	2205.0
67	1507.0
68	1127.5
69	748.0
70	660.5
71	573.0
72	490.0
73	407.0
74	336.5
75	193.0
76	120.0
77	82.0
78	44.0
79	38.0
80	32.0
81	22.0
82	12.0
83	8.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	721139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.6110574841174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24833419186825	28.618329188058176
2	12.480470935936646	9.887293033444859
3	4.71814081385939	5.606716409878342
4	2.324504299122677	3.6830429365850557
5	1.4575789150654712	2.886812109614793
6	1.0515813698036935	2.4992550053112614
7	0.7540789728181511	2.0908905877904793
8	0.5951792295794567	1.8860542940979652
9	0.5081750254331008	1.811641512998104
>10	3.6497440819503604	26.745774844214665
>50	0.13308838683963856	3.590437606380682
>100	0.06964350942461398	4.811177587551587
>500	0.004740134149319258	1.3696495770992043
>1k	0.004740134149319258	4.512925306974768
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	3907	0.541781820148404	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	3860	0.5352643526421398	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3627	0.5029543541536374	No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3483	0.48298594307061465	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	3135	0.43472894961997616	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3125	0.43334225440587737	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	2421	0.3357189113333213	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1845	0.25584526700123	No Hit
TCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1252	0.1736142408051707	No Hit
TCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1226	0.17000883324851382	No Hit
GCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1155	0.16016329722841227	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1134	0.15725123727880477	No Hit
CTGTCACTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	1076	0.1492084050370317	No Hit
CCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	947	0.13132003677515708	No Hit
GAATCAGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	881	0.12216784836210494	No Hit
TGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	801	0.11107428664931449	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	790	0.1095489219138058	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	786	0.10899424382816628	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	776	0.10760754861406747	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	738	0.10233810680049199	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3866952140988075E-4	0.0	0.0	0.5613342226671973	0.0
2	1.3866952140988075E-4	0.0	0.0	2.0964058246745774	0.0
3	1.3866952140988075E-4	0.0	0.0	2.9757092599346313	0.0
4	1.3866952140988075E-4	0.0	0.0	3.995623589904304	0.0
5	1.3866952140988075E-4	0.0	0.0	5.838957538005849	0.0
6	1.3866952140988075E-4	0.0	0.0	6.7756701551295935	0.0
7	1.3866952140988075E-4	0.0	0.0	8.133522108775146	0.0
8	1.3866952140988075E-4	0.0	0.0	10.277630248814722	0.0
9	1.3866952140988075E-4	0.0	0.0	11.203249304225677	0.0
10	1.3866952140988075E-4	0.0	0.0	13.307281952577798	0.0
11	1.3866952140988075E-4	0.0	0.0	16.818810243240208	0.0
12	1.3866952140988075E-4	0.0	0.0	20.62889401349809	0.0
13	1.3866952140988075E-4	0.0	0.0	21.58432701601217	0.0
14	1.3866952140988075E-4	0.0	0.0	21.87456232432305	0.0
15	1.3866952140988075E-4	0.0	0.0	22.3159474109707	0.0
16	1.3866952140988075E-4	0.0	0.0	23.230056896104635	0.0
17	1.3866952140988075E-4	0.0	0.0	24.78246218828825	0.0
18	1.3866952140988075E-4	0.0	0.0	26.380767092058534	0.0
19	1.3866952140988075E-4	0.0	0.0	27.76025149104403	0.0
20	1.3866952140988075E-4	0.0	0.0	28.911485857788858	0.0
21	1.3866952140988075E-4	0.0	0.0	30.517278915715277	0.0
22	1.3866952140988075E-4	0.0	0.0	32.383909343413684	0.0
23	1.3866952140988075E-4	0.0	0.0	34.010502829551584	0.0
24	1.3866952140988075E-4	0.0	0.0	35.21138088496115	0.0
25	1.3866952140988075E-4	0.0	0.0	36.208414743898196	0.0
26	1.3866952140988075E-4	0.0	0.0	37.09326496001464	0.0
27	1.3866952140988075E-4	0.0	0.0	37.97645114187417	0.0
28	1.3866952140988075E-4	0.0	0.0	38.89846478972847	0.0
29	1.3866952140988075E-4	0.0	0.0	39.846964316172055	0.0
30	1.3866952140988075E-4	0.0	0.0	40.89849529702318	0.0
31	1.3866952140988075E-4	0.0	0.0	41.85961374991506	0.0
32	1.3866952140988075E-4	0.0	0.0	42.7414132365605	0.0
33	1.3866952140988075E-4	0.0	0.0	43.583830579125525	0.0
34	1.3866952140988075E-4	0.0	0.0	44.46160864965007	0.0
35	1.3866952140988075E-4	0.0	0.0	45.37128071009889	0.0
36	1.3866952140988075E-4	0.0	0.0	46.2261783095908	0.0
37	1.3866952140988075E-4	0.0	0.0	47.04599252016602	0.0
38	1.3866952140988075E-4	0.0	0.0	47.91475707179892	0.0
39	1.3866952140988075E-4	0.0	0.0	48.92995663804066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGAT	30	2.1643718E-6	45.000004	45
TAGCGCG	30	2.1643718E-6	45.000004	1
TGCGGCC	25	3.889354E-5	45.000004	44
GCGTTAG	75	0.0	45.000004	1
ATCCGGG	25	3.889354E-5	45.000004	3
CCCGTAG	25	3.889354E-5	45.000004	34
ATAAGCG	25	3.889354E-5	45.000004	33
CGGGACG	30	2.1643718E-6	45.000004	6
TCCGTAG	25	3.889354E-5	45.000004	1
CAAGCGT	25	3.889354E-5	45.000004	17
TTATTCG	25	3.889354E-5	45.000004	1
CGGAGTT	25	3.889354E-5	45.000004	14
ATCGCTT	30	2.1643718E-6	45.000004	23
AACGTCA	40	6.8084773E-9	45.0	12
CGGGTAT	40	6.8084773E-9	45.0	6
AGCCGGT	20	7.0315367E-4	45.0	16
GCGAATC	20	7.0315367E-4	45.0	18
AACCCGA	20	7.0315367E-4	45.0	24
TTTAACG	20	7.0315367E-4	45.0	24
AACTTCG	20	7.0315367E-4	45.0	11
>>END_MODULE