##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934201.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675291
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72467869407411	34.0	31.0	34.0	31.0	34.0
2	32.916353098145834	34.0	31.0	34.0	31.0	34.0
3	32.94511699400703	34.0	33.0	34.0	31.0	34.0
4	36.312146911479644	37.0	37.0	37.0	35.0	37.0
5	35.33371391000324	37.0	35.0	37.0	35.0	37.0
6	25.748150056790333	37.0	0.0	37.0	0.0	37.0
7	30.986062304991478	37.0	19.0	37.0	17.0	37.0
8	34.93302739115433	37.0	32.0	37.0	32.0	37.0
9	37.52340102267023	39.0	37.0	39.0	35.0	39.0
10	37.771308961618026	39.0	37.0	39.0	35.0	39.0
11	37.821447346403254	39.0	37.0	39.0	35.0	39.0
12	37.51125514778074	39.0	37.0	39.0	35.0	39.0
13	37.494098099930255	39.0	37.0	39.0	35.0	39.0
14	38.51499131485537	40.0	38.0	41.0	35.0	41.0
15	38.71412324464564	40.0	38.0	41.0	35.0	41.0
16	38.869195650467724	40.0	38.0	41.0	35.0	41.0
17	38.879653364253336	40.0	38.0	41.0	35.0	41.0
18	38.84795443742031	40.0	38.0	41.0	35.0	41.0
19	38.830481969995155	40.0	38.0	41.0	35.0	41.0
20	38.737631628438706	40.0	38.0	41.0	35.0	41.0
21	38.65619118276417	40.0	38.0	41.0	35.0	41.0
22	38.662064206393985	40.0	37.0	41.0	35.0	41.0
23	38.62364521369306	40.0	37.0	41.0	35.0	41.0
24	38.591305081809175	40.0	37.0	41.0	35.0	41.0
25	38.49032491177877	40.0	37.0	41.0	35.0	41.0
26	38.45825725502043	40.0	37.0	41.0	35.0	41.0
27	38.42103626436603	40.0	37.0	41.0	35.0	41.0
28	38.36137457777462	40.0	37.0	41.0	35.0	41.0
29	38.33148968370673	40.0	37.0	41.0	35.0	41.0
30	38.101141581925425	40.0	36.0	41.0	34.0	41.0
31	38.078155935737335	40.0	36.0	41.0	34.0	41.0
32	38.01467515485916	40.0	36.0	41.0	34.0	41.0
33	37.91598584906359	40.0	36.0	41.0	34.0	41.0
34	37.77414477610393	40.0	36.0	41.0	34.0	41.0
35	37.70862487431344	40.0	36.0	41.0	33.0	41.0
36	37.622149562188746	40.0	36.0	41.0	33.0	41.0
37	37.57332616605286	40.0	35.0	41.0	33.0	41.0
38	37.50785661292687	40.0	35.0	41.0	33.0	41.0
39	37.457826329686014	40.0	35.0	41.0	33.0	41.0
40	37.368735848693376	40.0	35.0	41.0	33.0	41.0
41	37.24889714212095	40.0	35.0	41.0	33.0	41.0
42	37.21324732596762	40.0	35.0	41.0	33.0	41.0
43	37.16212862306769	40.0	35.0	41.0	33.0	41.0
44	37.057632931580606	39.0	35.0	41.0	33.0	41.0
45	37.02925553576162	39.0	35.0	41.0	33.0	41.0
46	37.00736275176183	39.0	35.0	41.0	33.0	41.0
47	36.92456881551805	39.0	35.0	41.0	33.0	41.0
48	36.861958770367146	39.0	35.0	41.0	32.0	41.0
49	36.83247222308605	39.0	35.0	41.0	32.0	41.0
50	36.74680248959338	39.0	35.0	41.0	32.0	41.0
51	35.771353386910235	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	8.0
11	3.0
12	5.0
13	6.0
14	9.0
15	15.0
16	24.0
17	49.0
18	79.0
19	149.0
20	260.0
21	436.0
22	704.0
23	1154.0
24	1886.0
25	3051.0
26	4246.0
27	4717.0
28	5128.0
29	5419.0
30	6568.0
31	8990.0
32	14201.0
33	26866.0
34	42407.0
35	48573.0
36	54801.0
37	99024.0
38	154201.0
39	192145.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.060859688637933	4.014417488164361	56.221836215794376	17.702886607403325
2	20.819468940056954	4.1476933647864405	53.717434409758155	21.315403285398443
3	20.59008634795962	4.130663669440286	53.64590968930432	21.63334029329578
4	19.006472765074612	4.273861194655342	52.48833465868788	24.231331381582162
5	18.297297017137797	7.912736879360158	49.720490869862026	24.069475233640016
6	16.40492765341164	32.125113469600514	38.60898486726463	12.860974009723217
7	85.13396446865129	1.8535712751983962	9.278074193199673	3.7343900629506392
8	87.62814845747981	1.9834412127512433	7.138404036185881	3.2500062935830623
9	82.00775665601941	4.464445698224914	9.087193521015385	4.440604124740298
10	42.34811362805072	29.471886934669644	15.718853057422653	12.46114637985698
11	30.75222385608575	22.80216973127141	28.286620138577295	18.15898627406555
12	28.567832238249878	21.053590229989737	30.461386276434897	19.91719125532548
13	27.179541856769895	20.50138384785226	32.827329255091506	19.491745040286336
14	20.436522921229514	23.418940871416915	35.094796169355135	21.049740037998433
15	19.256883328816762	23.860972528880144	36.50337410094315	20.378770041359946
16	25.877880795094267	23.223469585704535	31.305911081296806	19.5927385379044
17	25.654273490983886	23.294253884621593	29.49957870014557	21.55189392424895
18	25.681521003537732	22.515922765148655	31.271851690604496	20.530704540709117
19	25.61280988492369	25.005812309063796	29.168314104586024	20.213063701426496
20	25.28658015581431	28.731169229265607	27.9035260354425	18.078724579477587
21	25.51462998914542	25.27073513492702	31.130283092770377	18.08435178315719
22	23.44855773288849	22.393160874348986	32.56211026061357	21.596171132148957
23	22.055824822187766	23.79566734933532	32.337318282044336	21.811189546432573
24	23.070794664818575	24.94376498428085	30.080069184988396	21.905371165912175
25	24.27649709532631	24.99144813125008	28.160008055786317	22.572046717637285
26	21.708567121433575	25.791695728211984	28.507858093769944	23.991879056584494
27	20.428822537246905	25.066230706465802	32.016863840921914	22.488082915365375
28	19.08984423011709	25.95325570753942	30.784210066475044	24.17268999586845
29	21.264018030745262	25.65190414206616	30.26991326702118	22.814164560167395
30	24.179205705392192	24.20645321794604	30.206385099164656	21.40795597749711
31	23.76545815063432	24.433466461125647	30.113980491373347	21.687094896866686
32	25.658567935897263	24.798050025840713	27.722418927543828	21.820963110718196
33	25.855964317605302	24.101609528336674	28.09766456238866	21.94476159166937
34	21.79564069416	24.47552240441528	31.139316235519203	22.589520665905514
35	21.543453118729555	26.368187936756154	29.35860243954088	22.729756504973412
36	23.721921364271108	26.670872260995633	28.12328314756157	21.483923227171694
37	21.873977292752308	28.12742950816759	27.781504566179617	22.217088632900484
38	23.542443183753374	25.859962593903962	27.71886490416724	22.878729318175424
39	23.021926843390478	24.641228744348734	27.02716310449865	25.309681307762133
40	25.183957730815308	23.25086518256574	28.120617630029127	23.444559456589825
41	21.756546437017523	24.77183910343837	27.46697349735151	26.004640962192592
42	21.37656210433724	24.954427054410615	30.027055002954285	23.64195583829786
43	20.972588113864983	24.48944232930692	30.66870430673591	23.869265250092184
44	22.082035744590108	23.727252399335992	29.102860840733847	25.08785101534005
45	21.60342726320949	23.275447177587143	28.153936599184647	26.967188960018717
46	21.977043970673385	23.959596677580482	29.199559893438533	24.863799458307604
47	19.769106947967614	24.980341808198244	31.499457271013533	23.751093972820605
48	20.461549169172994	26.084162235243767	30.054746768430203	23.399541827153033
49	22.790026818068064	23.764569644790175	30.259843534120844	23.185560003020917
50	21.312293514943928	24.159066239591525	28.484460773207402	26.044179472257145
51	20.421418321879013	24.52824041783468	28.018883710874277	27.031457549412032
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	165.0
1	473.5
2	782.0
3	3682.0
4	6582.0
5	4196.0
6	1810.0
7	1744.0
8	1678.0
9	1732.5
10	1787.0
11	1816.0
12	1845.0
13	1783.0
14	1721.0
15	1694.5
16	1668.0
17	1639.5
18	1611.0
19	1789.0
20	1967.0
21	2108.5
22	2250.0
23	2773.5
24	3297.0
25	3926.5
26	5624.0
27	6692.0
28	7622.0
29	8552.0
30	9994.0
31	11436.0
32	13505.5
33	15575.0
34	17370.0
35	19165.0
36	20517.5
37	21870.0
38	24801.5
39	27733.0
40	29798.0
41	31863.0
42	34370.0
43	36877.0
44	40175.5
45	43474.0
46	48592.0
47	53710.0
48	58840.0
49	63970.0
50	62625.5
51	61281.0
52	54632.0
53	47983.0
54	44492.0
55	41001.0
56	38467.5
57	35934.0
58	34296.0
59	32658.0
60	28621.0
61	24584.0
62	21684.5
63	18785.0
64	16321.0
65	13857.0
66	11738.5
67	9620.0
68	7909.5
69	6199.0
70	5286.5
71	4374.0
72	3583.0
73	2792.0
74	2241.5
75	1293.0
76	895.0
77	707.5
78	520.0
79	383.0
80	246.0
81	192.5
82	139.0
83	99.5
84	60.0
85	42.0
86	24.0
87	16.5
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	675291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.09877493130691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.70720038893255	27.645649140296904
2	8.937934799781004	6.9892460217466645
3	4.029282382997098	4.726200150824506
4	2.7869176316779325	4.358602609322655
5	2.230330282343687	4.360159086591701
6	1.8287494643347582	4.2901118227063675
7	1.4644551191470028	4.008088077037023
8	1.3062314653906197	4.085764005879924
9	1.0796034557214553	3.7990055277282915
>10	5.560685670342109	31.73516735785681
>50	0.0449371113613579	1.1903580135734826
>100	0.020462434347346983	1.4504546745402132
>500	0.0020061210144457805	0.5540780781393067
>1k	0.0012036726086674682	0.8071154337561531
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2912	0.43122150302610285	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1176	0.1741471454528492	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1057	0.15652511287726328	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	946	0.14008775476054028	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	869	0.12868526309398465	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4808430735786498E-4	0.0	0.0	0.13357204523679422	0.0
2	1.4808430735786498E-4	0.0	0.0	0.4778680598438303	0.0
3	1.4808430735786498E-4	0.0	0.0	0.7195416494518659	0.0
4	1.4808430735786498E-4	0.0	0.0	1.0040116038863245	0.0
5	1.4808430735786498E-4	0.0	0.0	1.561697105396044	0.0
6	1.4808430735786498E-4	0.0	0.0	2.075993904849909	0.0
7	1.4808430735786498E-4	0.0	0.0	2.7017981877442465	0.0
8	1.4808430735786498E-4	0.0	0.0	3.600373764791771	0.0
9	1.4808430735786498E-4	0.0	0.0	4.175088961647646	0.0
10	1.4808430735786498E-4	0.0	0.0	5.046417026141323	0.0
11	1.4808430735786498E-4	0.0	0.0	6.3898378624918735	0.0
12	1.4808430735786498E-4	0.0	0.0	7.5337891368313805	0.0
13	1.4808430735786498E-4	0.0	0.0	7.923250865182566	0.0
14	1.4808430735786498E-4	0.0	0.0	8.13664035208525	0.0
15	1.4808430735786498E-4	0.0	0.0	8.34603156268927	0.0
16	1.4808430735786498E-4	0.0	0.0	8.754448082382261	0.0
17	1.4808430735786498E-4	0.0	0.0	9.323684159865895	0.0
18	1.4808430735786498E-4	0.0	0.0	9.936308939405382	0.0
19	1.4808430735786498E-4	0.0	0.0	10.422321636153896	0.0
20	1.4808430735786498E-4	0.0	0.0	10.939728206062275	0.0
21	1.4808430735786498E-4	0.0	0.0	11.609661612549257	0.0
22	1.4808430735786498E-4	0.0	0.0	12.356154605940254	0.0
23	1.4808430735786498E-4	0.0	0.0	13.064738016647638	0.0
24	1.4808430735786498E-4	0.0	0.0	13.616796314477758	0.0
25	1.4808430735786498E-4	0.0	0.0	14.113471081356037	0.0
26	1.4808430735786498E-4	0.0	0.0	14.567941820637325	0.0
27	1.4808430735786498E-4	0.0	0.0	15.091123678532663	0.0
28	1.4808430735786498E-4	0.0	0.0	15.567214726688198	0.0
29	1.4808430735786498E-4	0.0	0.0	16.131415937721663	0.0
30	1.4808430735786498E-4	0.0	0.0	16.809049728191255	0.0
31	1.4808430735786498E-4	0.0	0.0	17.330010321476223	0.0
32	1.4808430735786498E-4	0.0	0.0	17.83394121941504	0.0
33	1.4808430735786498E-4	0.0	0.0	18.34912652471305	0.0
34	1.4808430735786498E-4	0.0	0.0	18.917326012045176	0.0
35	1.4808430735786498E-4	0.0	0.0	19.52906228574052	0.0
36	1.4808430735786498E-4	0.0	0.0	20.066756405756927	0.0
37	1.4808430735786498E-4	0.0	0.0	20.604598610080693	0.0
38	1.4808430735786498E-4	0.0	0.0	21.266091211048273	0.0
39	1.4808430735786498E-4	0.0	0.0	22.476384255084106	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAGG	25	3.8890903E-5	45.0	2
CCGTTAG	20	7.0312177E-4	45.0	1
TGCGTAG	40	6.8084773E-9	45.0	1
CTCGTAG	85	0.0	42.35294	1
TCGTTTG	160	0.0	42.1875	1
CGTTTTT	1295	0.0	41.872585	1
TAGGGAT	1210	0.0	41.09504	5
GCGTAGG	55	6.002665E-11	40.909092	2
TAGGGAC	810	0.0	40.833332	5
TTTCGCG	160	0.0	40.78125	1
CGTTAGG	205	0.0	40.609756	2
TTTAGGG	2675	0.0	40.542057	3
TATTGCG	50	1.0804797E-9	40.5	1
CGTTTAT	600	0.0	40.5	1
TCGCGCG	50	1.0804797E-9	40.5	1
TTAGGGA	1790	0.0	40.34916	4
TCAGGGA	810	0.0	40.277775	4
TTAGGGG	2455	0.0	40.234215	4
TCTTAGG	745	0.0	40.167786	2
GCTAGGG	785	0.0	40.12739	3
>>END_MODULE