FastQCFastQC Report
Sat 14 Jan 2017
SRR2934200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512606
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC22600.4408844219537032TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC22270.4344467290667686TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT22230.4336664026562311TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC16660.3250059499888803TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.31408138024135496No Hit
TCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG8590.1675750966629341TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC7750.15118824204164602TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC7590.14806693639949592TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG6720.13109483697030466TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCACTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT6220.12134075683858558TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC6100.11899977760697299TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.11743912478589794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG302.1631404E-645.00000425
ACACCCG600.045.00000413
ATCCGGC302.1631404E-645.00000413
CTGTTCG302.1631404E-645.0000041
CACGTGA302.1631404E-645.00000443
TAGTGGT302.1631404E-645.00000415
CGCCGGT302.1631404E-645.00000428
TCCGGAC253.887771E-545.018
CTCGTTG253.887771E-545.01
GTCGATT253.887771E-545.013
CGACGAA207.029625E-445.018
CGTGCAC253.887771E-545.016
GGCACGT253.887771E-545.011
GGGTACG406.8030204E-945.07
GCCGGTC207.029625E-445.09
CCGTAGG207.029625E-445.02
ACCTACG207.029625E-445.033
CGCTCCC207.029625E-445.024
GCTTCGT207.029625E-445.022
AGGCACG351.2101737E-745.010
Produced by FastQC (version 0.11.5)