##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512606
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26441165339462	33.0	31.0	34.0	30.0	34.0
2	32.428485035290265	34.0	31.0	34.0	31.0	34.0
3	32.47947936621889	34.0	31.0	34.0	30.0	34.0
4	35.98309617913173	37.0	35.0	37.0	35.0	37.0
5	35.05927554496046	37.0	35.0	37.0	35.0	37.0
6	25.69911589017686	36.0	0.0	37.0	0.0	37.0
7	30.906868042902346	37.0	19.0	37.0	17.0	37.0
8	34.70783993944667	37.0	32.0	37.0	32.0	37.0
9	37.26854933418649	39.0	37.0	39.0	35.0	39.0
10	37.26445652216322	39.0	37.0	39.0	34.0	39.0
11	37.30987737170459	39.0	37.0	39.0	34.0	39.0
12	37.1525694197883	39.0	37.0	39.0	34.0	39.0
13	37.17952384482429	39.0	37.0	39.0	34.0	39.0
14	38.31353515175398	40.0	38.0	41.0	34.0	41.0
15	38.438877032262596	40.0	38.0	41.0	34.0	41.0
16	38.5391216645923	40.0	38.0	41.0	35.0	41.0
17	38.523005973398675	40.0	38.0	41.0	34.0	41.0
18	38.51897363667222	40.0	38.0	41.0	35.0	41.0
19	38.51237597687113	40.0	38.0	41.0	34.0	41.0
20	38.43180922579915	40.0	37.0	41.0	34.0	41.0
21	38.326492471800954	40.0	37.0	41.0	34.0	41.0
22	38.324122230328946	40.0	37.0	41.0	34.0	41.0
23	38.26211749374763	40.0	37.0	41.0	34.0	41.0
24	38.22696378895292	40.0	37.0	41.0	34.0	41.0
25	38.12400947316262	40.0	37.0	41.0	34.0	41.0
26	38.09645224597449	40.0	37.0	41.0	34.0	41.0
27	38.08325887718833	40.0	37.0	41.0	34.0	41.0
28	38.02338638252381	40.0	37.0	41.0	34.0	41.0
29	37.99957277129023	40.0	37.0	41.0	34.0	41.0
30	37.7611303808383	40.0	36.0	41.0	33.0	41.0
31	37.78534586017331	40.0	36.0	41.0	33.0	41.0
32	37.69237777162187	40.0	36.0	41.0	33.0	41.0
33	37.62613976426339	40.0	36.0	41.0	33.0	41.0
34	37.565830286808975	40.0	36.0	41.0	33.0	41.0
35	37.550018922915456	40.0	36.0	41.0	33.0	41.0
36	37.49258494828387	40.0	36.0	41.0	33.0	41.0
37	37.418393463985986	40.0	36.0	41.0	33.0	41.0
38	37.30131328934893	40.0	36.0	41.0	33.0	41.0
39	37.30031642235947	40.0	36.0	41.0	33.0	41.0
40	37.244417739940616	40.0	36.0	41.0	32.0	41.0
41	37.15561854523747	40.0	35.0	41.0	32.0	41.0
42	37.1472007740838	40.0	35.0	41.0	32.0	41.0
43	37.093229497898975	39.0	35.0	41.0	32.0	41.0
44	36.96199420217477	39.0	35.0	41.0	32.0	41.0
45	36.94437443182483	39.0	35.0	41.0	31.0	41.0
46	36.8750151188242	39.0	35.0	41.0	31.0	41.0
47	36.798193934522814	39.0	35.0	41.0	31.0	41.0
48	36.801910239052994	39.0	35.0	41.0	31.0	41.0
49	36.80132889587715	39.0	35.0	41.0	31.0	41.0
50	36.71836069027674	39.0	35.0	41.0	31.0	41.0
51	35.72477302255533	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	3.0
12	3.0
13	4.0
14	4.0
15	7.0
16	13.0
17	30.0
18	62.0
19	136.0
20	221.0
21	366.0
22	647.0
23	990.0
24	1689.0
25	2583.0
26	3471.0
27	4042.0
28	4527.0
29	5394.0
30	6672.0
31	8991.0
32	12630.0
33	20037.0
34	30392.0
35	41201.0
36	48319.0
37	81884.0
38	115980.0
39	122227.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.468121715313515	4.290039523532694	56.640967916879625	15.600870844274159
2	20.988049301022617	4.333347639317527	53.29961022695794	21.37899283270192
3	19.590094536544637	3.985126978615155	54.69307811457532	21.731700370264882
4	19.043280804360464	4.450396600898156	52.32576286660711	24.18055972813428
5	16.58486244796198	7.908022925989941	51.95023078153591	23.556883844512157
6	14.360932177930028	31.502557519810537	41.30891952103565	12.827590781223785
7	82.5497555627519	1.4783283847633464	10.947979539841516	5.023936512643239
8	84.39425211565998	1.5852331030069877	8.667085441840323	5.35342933949271
9	79.45381052894426	4.06862190454267	10.96729261850232	5.510274948010753
10	37.93049632661342	28.779413428637202	18.839030366402266	14.451059878347113
11	25.174500493556458	25.144457926750757	29.67425273992111	20.006788839771676
12	24.307362769846627	23.514941299945768	32.60867020674748	19.569025723460122
13	22.51339235202085	26.068364396827192	33.446935853267426	17.971307397884534
14	18.899505663218925	28.779608510239836	31.57707869201687	20.743807134524374
15	15.917683366952396	27.556641943324895	36.27952072351865	20.246153966204066
16	18.861854913910488	25.656742215268647	32.928213871862596	22.553188998958266
17	18.395609883614316	25.73262895869342	29.861531078450117	26.01023007924215
18	19.629110857071513	24.720740685828883	33.168164243102886	22.481984213996714
19	21.586754739507537	27.948170719812097	29.711903489229545	20.75317105145082
20	23.33878261276692	27.223832729230637	30.316851538998762	19.12053311900368
21	21.924245912065015	28.554289259197123	30.65102632431146	18.870438504426403
22	20.53487473810295	26.16219864769433	29.120416070042097	24.182510544160625
23	18.3801984370062	27.28606376047101	29.262630558362563	25.071107244160235
24	20.234449070046	25.344806732656274	31.064599321896345	23.35614487540138
25	18.245592131188477	27.89627901351135	28.13876544558589	25.719363409714287
26	17.468191944690464	29.11514886677097	28.794239630437414	24.622419558101154
27	18.09303831792839	27.42379137193088	31.336738157571315	23.14643215256942
28	16.110423990355166	25.634307830965692	32.604768574694795	25.650499603984343
29	16.59364112008053	24.72054560422625	31.827173306594148	26.858639969099073
30	18.588155425414453	25.569345657288444	31.004904351490225	24.837594565806878
31	18.46935072941011	28.131352344685784	28.49010741193041	24.909189513973693
32	18.30489693838933	29.506872724860813	27.356683300624653	24.83154703612521
33	18.492565440123602	27.17486724696941	28.02659352407112	26.305973788835868
34	15.78034591869779	26.258178796190446	30.024619298252457	27.936855986859303
35	15.58623972407658	26.435898136190367	28.415000994916173	29.56286114481688
36	17.818168339816545	26.649902654280282	28.613008821590068	26.9189201843131
37	16.820521023944316	27.238073686222947	31.01134204437716	24.930063245455575
38	16.429577492265015	30.34923508503607	27.28411294444466	25.937074478254253
39	18.27875600363632	26.83308427915397	29.04979652988845	25.838363187321256
40	19.4032063612209	25.06974167294179	30.26183852705587	25.26521343878144
41	16.092671564515438	26.542217609626107	29.444251530415173	27.920859295443286
42	18.03197777630383	25.602314448133654	27.65886470310531	28.70684307245721
43	18.870048341221132	24.815940507914462	28.869150965849016	27.44486018501539
44	17.81134048372434	25.583001369472854	27.171355778121985	29.43430236868082
45	18.352887012637385	24.395929817442635	26.64970757267765	30.601475597242327
46	18.52572931257145	25.203957815554247	29.543548066156074	26.726764805718233
47	15.16857001283637	26.03656609559779	32.191585740315176	26.60327815125067
48	15.831652380190633	24.649340819264697	31.298892326660244	28.22011447388443
49	17.993741782187488	22.440626914238226	31.278018595178363	28.28761270839592
50	17.42351825768719	23.047915943239055	30.035153704794716	29.493412094279037
51	15.741329598170914	23.624577160626288	27.617117240141553	33.016976001061245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	189.0
2	326.0
3	1864.5
4	3403.0
5	2263.0
6	1123.0
7	1125.0
8	1127.0
9	1198.0
10	1269.0
11	1276.0
12	1283.0
13	1384.0
14	1485.0
15	1461.0
16	1437.0
17	1422.5
18	1408.0
19	1519.0
20	1630.0
21	1858.5
22	2087.0
23	2268.0
24	2449.0
25	2873.0
26	4013.0
27	4729.0
28	5522.5
29	6316.0
30	7307.0
31	8298.0
32	9846.5
33	11395.0
34	13751.5
35	16108.0
36	16932.5
37	17757.0
38	19753.0
39	21749.0
40	24452.0
41	27155.0
42	31384.0
43	35613.0
44	38959.0
45	42305.0
46	49696.5
47	57088.0
48	63817.0
49	70546.0
50	64636.5
51	58727.0
52	47285.5
53	35844.0
54	29336.0
55	22828.0
56	19074.0
57	15320.0
58	13773.5
59	12227.0
60	10422.5
61	8618.0
62	7737.0
63	6856.0
64	5661.5
65	4467.0
66	3336.5
67	2206.0
68	1842.5
69	1479.0
70	1307.0
71	1135.0
72	826.0
73	517.0
74	507.5
75	328.0
76	158.0
77	125.0
78	92.0
79	84.0
80	76.0
81	85.0
82	94.0
83	56.5
84	19.0
85	13.5
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	512606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.06774257826633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.33267892547106	29.382749017604308
2	11.755179279116632	9.420069946340313
3	4.177036166206956	5.020932295430665
4	2.069088774732409	3.3161486559023463
5	1.3348175278703207	2.674156254783293
6	0.9357151751796189	2.2495196859404616
7	0.7483312628500214	2.098876108220053
8	0.5918628398260016	1.8971686326231936
9	0.5344435889080602	1.9272553328675661
>10	4.374028123622421	33.48082154397861
>50	0.09854929417272901	2.715098251490042
>100	0.042235411788285694	2.8184480625011066
>500	0.003519617649023808	0.9869604759251551
>1k	0.0025140126064455773	2.011795736392856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	2260	0.4408844219537032	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	2227	0.4344467290667686	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	2223	0.4336664026562311	TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1666	0.3250059499888803	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1610	0.31408138024135496	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	859	0.1675750966629341	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	775	0.15118824204164602	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	759	0.14806693639949592	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	672	0.13109483697030466	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCACTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	622	0.12134075683858558	TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	610	0.11899977760697299	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.11743912478589794	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9508160263438196E-4	0.0	0.0	0.45493029734337875	0.0
2	1.9508160263438196E-4	0.0	0.0	1.7455901803724498	0.0
3	1.9508160263438196E-4	0.0	0.0	2.516357592380893	0.0
4	1.9508160263438196E-4	0.0	0.0	3.267226680920629	0.0
5	1.9508160263438196E-4	0.0	0.0	4.51204238733062	0.0
6	1.9508160263438196E-4	0.0	0.0	5.328458894355509	0.0
7	1.9508160263438196E-4	0.0	0.0	6.29235709297199	0.0
8	1.9508160263438196E-4	0.0	0.0	7.872518074310484	0.0
9	1.9508160263438196E-4	0.0	0.0	8.50672836447486	0.0
10	1.9508160263438196E-4	0.0	0.0	9.839330792070323	0.0
11	1.9508160263438196E-4	0.0	0.0	12.670745172705743	0.0
12	1.9508160263438196E-4	0.0	0.0	14.844734552463295	0.0
13	1.9508160263438196E-4	0.0	0.0	15.581362684010722	0.0
14	1.9508160263438196E-4	0.0	0.0	15.833408114614343	0.0
15	1.9508160263438196E-4	0.0	0.0	16.145148515624086	0.0
16	1.9508160263438196E-4	0.0	0.0	17.110217203856372	0.0
17	1.9508160263438196E-4	0.0	0.0	18.59966523996988	0.0
18	1.9508160263438196E-4	0.0	0.0	20.19348193349278	0.0
19	1.9508160263438196E-4	0.0	0.0	21.202639063920437	0.0
20	1.9508160263438196E-4	0.0	0.0	22.17648642427127	0.0
21	1.9508160263438196E-4	0.0	0.0	23.658716441087307	0.0
22	1.9508160263438196E-4	0.0	0.0	25.14094645790334	0.0
23	1.9508160263438196E-4	0.0	0.0	26.776705695992632	0.0
24	1.9508160263438196E-4	0.0	0.0	28.012742730284078	0.0
25	1.9508160263438196E-4	0.0	0.0	29.001416292435124	0.0
26	1.9508160263438196E-4	0.0	0.0	29.848070447868345	0.0
27	1.9508160263438196E-4	0.0	0.0	30.678532830282908	0.0
28	1.9508160263438196E-4	0.0	0.0	31.548791859634886	0.0
29	1.9508160263438196E-4	0.0	0.0	32.547414583520286	0.0
30	1.9508160263438196E-4	0.0	0.0	33.54369632817408	0.0
31	1.9508160263438196E-4	0.0	0.0	34.48906177454029	0.0
32	1.9508160263438196E-4	0.0	0.0	35.32440119702071	0.0
33	1.9508160263438196E-4	0.0	0.0	36.13028329750335	0.0
34	1.9508160263438196E-4	0.0	0.0	36.957429292673126	0.0
35	1.9508160263438196E-4	0.0	0.0	37.8395882997858	0.0
36	1.9508160263438196E-4	0.0	0.0	38.64683597148687	0.0
37	1.9508160263438196E-4	0.0	0.0	39.45876560165117	0.0
38	1.9508160263438196E-4	0.0	0.0	40.25684443802843	0.0
39	1.9508160263438196E-4	0.0	0.0	41.09998712461422	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCGG	30	2.1631404E-6	45.000004	25
ACACCCG	60	0.0	45.000004	13
ATCCGGC	30	2.1631404E-6	45.000004	13
CTGTTCG	30	2.1631404E-6	45.000004	1
CACGTGA	30	2.1631404E-6	45.000004	43
TAGTGGT	30	2.1631404E-6	45.000004	15
CGCCGGT	30	2.1631404E-6	45.000004	28
TCCGGAC	25	3.887771E-5	45.0	18
CTCGTTG	25	3.887771E-5	45.0	1
GTCGATT	25	3.887771E-5	45.0	13
CGACGAA	20	7.029625E-4	45.0	18
CGTGCAC	25	3.887771E-5	45.0	16
GGCACGT	25	3.887771E-5	45.0	11
GGGTACG	40	6.8030204E-9	45.0	7
GCCGGTC	20	7.029625E-4	45.0	9
CCGTAGG	20	7.029625E-4	45.0	2
ACCTACG	20	7.029625E-4	45.0	33
CGCTCCC	20	7.029625E-4	45.0	24
GCTTCGT	20	7.029625E-4	45.0	22
AGGCACG	35	1.2101737E-7	45.0	10
>>END_MODULE