FastQCFastQC Report
Sat 14 Jan 2017
SRR2934199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970416
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC162201.671448121218117No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG143931.4831783482547691No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC103791.0695413101185471No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC42450.43744126230400154No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCT35500.365822492621721No Hit
GAATCAGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC35290.36365847224283193No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTC34550.3560328766219848No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT31160.32109940479134724No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.3015201727918748No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGCATT29100.2998713953603403No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC27960.2881238561606569No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTA26560.27369705363472985No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT22970.2367026100146741No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC20090.2070246162470528No Hit
GCTGTATCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC16140.16632042340604442No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG11030.11366259418641078No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.11057113650228356No Hit
CTGTCACTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT10650.1097467477865163No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGT10600.10923150483916177No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC9870.10170895780778552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA302.1651504E-645.00000416
AACGCGT253.8903545E-545.0000049
ACTACGG253.8903545E-545.0000045
GCTACTA253.8903545E-545.00000428
CCGCGCG253.8903545E-545.00000417
CGCACGA253.8903545E-545.00000425
GCGTACG302.1651504E-645.0000041
TAATACG453.8380676E-1045.04
TAGCCGT207.032744E-445.044
AAGTTCG207.032744E-445.020
CCAACCG207.032744E-445.038
CATCCGT207.032744E-445.034
TCGTTAG1200.043.1250041
GCGTTAG1150.043.0434761
CTAGGGA9850.042.4873124
CCCGAAC650.041.53846431
TAGGGAG9850.041.345185
TACGGCT19450.041.29827
GGCGATA603.6379788E-1241.2500048
CGTTTTT13600.041.194851
Produced by FastQC (version 0.11.5)