##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1667279
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34425252162355	33.0	31.0	34.0	31.0	34.0
2	32.52089962147907	34.0	31.0	34.0	31.0	34.0
3	32.55677364136416	34.0	31.0	34.0	31.0	34.0
4	36.03927597000862	37.0	35.0	37.0	35.0	37.0
5	35.14476521326065	37.0	35.0	37.0	35.0	37.0
6	25.986752667070117	37.0	0.0	37.0	0.0	37.0
7	31.046670653202014	37.0	19.0	37.0	17.0	37.0
8	34.76056017019347	37.0	32.0	37.0	32.0	37.0
9	37.29660782628462	39.0	37.0	39.0	35.0	39.0
10	37.39213472970031	39.0	37.0	39.0	35.0	39.0
11	37.40357312723305	39.0	37.0	39.0	35.0	39.0
12	36.98395049658755	39.0	35.0	39.0	33.0	39.0
13	36.94571454447636	39.0	35.0	39.0	33.0	39.0
14	37.81261864391023	40.0	37.0	41.0	33.0	41.0
15	38.02266207395403	40.0	37.0	41.0	33.0	41.0
16	38.1906369599809	40.0	37.0	41.0	33.0	41.0
17	38.18843636847822	40.0	37.0	41.0	33.0	41.0
18	38.156579672628276	40.0	37.0	41.0	34.0	41.0
19	38.14770353372171	40.0	37.0	41.0	34.0	41.0
20	38.04854556435966	40.0	36.0	41.0	33.0	41.0
21	37.952296526256255	40.0	36.0	41.0	33.0	41.0
22	37.99111786329703	40.0	36.0	41.0	34.0	41.0
23	37.95175972347759	40.0	35.0	41.0	34.0	41.0
24	37.89408971144002	40.0	35.0	41.0	34.0	41.0
25	37.7772874246002	40.0	35.0	41.0	34.0	41.0
26	37.745353956956215	40.0	35.0	41.0	34.0	41.0
27	37.723147115749676	40.0	35.0	41.0	34.0	41.0
28	37.63513785035378	39.0	35.0	41.0	33.0	41.0
29	37.5976474243363	39.0	35.0	41.0	33.0	41.0
30	37.331318873445895	39.0	35.0	41.0	33.0	41.0
31	37.300070953931524	39.0	35.0	41.0	33.0	41.0
32	37.23579436914878	39.0	35.0	41.0	33.0	41.0
33	37.12919433400169	39.0	35.0	41.0	32.0	41.0
34	36.941607253495064	39.0	35.0	41.0	31.0	41.0
35	36.87108696264992	39.0	35.0	41.0	31.0	41.0
36	36.81110180119824	39.0	35.0	41.0	31.0	41.0
37	36.74820710870826	39.0	35.0	41.0	31.0	41.0
38	36.67068139165671	39.0	35.0	41.0	31.0	41.0
39	36.61709767831299	39.0	35.0	41.0	31.0	41.0
40	36.516476246626986	39.0	35.0	41.0	31.0	41.0
41	36.3737472852474	39.0	35.0	41.0	31.0	41.0
42	36.32816103363624	39.0	35.0	41.0	31.0	41.0
43	36.26674059950374	39.0	35.0	40.0	31.0	41.0
44	36.1513202049567	38.0	35.0	40.0	30.0	41.0
45	36.116702123639776	38.0	35.0	40.0	30.0	41.0
46	36.06729887439355	38.0	35.0	40.0	30.0	41.0
47	35.97936158255457	38.0	35.0	40.0	30.0	41.0
48	35.92232493781785	38.0	35.0	40.0	30.0	41.0
49	35.89414189226878	38.0	35.0	40.0	30.0	41.0
50	35.792173955288824	38.0	35.0	40.0	30.0	41.0
51	34.76909203558613	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	15.0
11	12.0
12	11.0
13	17.0
14	24.0
15	55.0
16	118.0
17	207.0
18	369.0
19	663.0
20	1142.0
21	1942.0
22	2772.0
23	4524.0
24	7163.0
25	10514.0
26	14337.0
27	16905.0
28	19099.0
29	22311.0
30	27248.0
31	36522.0
32	51658.0
33	86987.0
34	127175.0
35	137375.0
36	154461.0
37	250723.0
38	338813.0
39	353956.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.364415913593344	4.205294974626322	54.602379085923836	17.8279100258565
2	21.9662096145876	4.032258548209388	52.60961122883453	21.391920608368487
3	21.042668923437528	4.230125851762063	52.58262114499133	22.14458407980908
4	18.986744270155146	4.811132389959929	51.63412962077732	24.5679937191076
5	18.011562551918423	7.9947027462110425	49.64831920752316	24.345415494347378
6	16.249949768455068	31.03385816051183	39.337747311637706	13.378444759395396
7	83.33494274203657	2.076497094967309	10.232180696811993	4.356379466184124
8	85.15047571522223	2.1854170777656288	8.495458768448472	4.168648438563672
9	79.90624244652514	3.981637146512371	10.715783021317968	5.396337385644514
10	45.53371091460997	21.54294512196219	17.688401281369227	15.23494268205861
11	34.492367504178965	22.35084829833519	24.91190736523401	18.24487683225183
12	30.74968256662502	19.98993569762469	29.196073362646562	20.064308373103724
13	28.087140784475785	19.25802460176131	32.186334740616296	20.468499873146605
14	21.47307079379036	20.89440339619224	35.82891645609403	21.803609353923367
15	20.48517374716529	20.989648403176673	37.230721432945536	21.2944564167125
16	26.918770043885875	21.35341475541886	31.520819251007183	20.206995949688082
17	26.59135033788586	21.877742117546013	29.84653438326759	21.684373161300538
18	26.66602290318537	21.342438787989295	31.21349216297932	20.778046145846016
19	26.051488683057844	24.312007768345907	29.178379863238245	20.458123685357997
20	25.273634466696937	28.00491099570018	28.05427286015118	18.667181677451705
21	25.55882968597337	25.496992404990404	30.530643041746465	18.41353486728976
22	23.668144323775444	21.97982461243739	32.666698255061085	21.685332808726073
23	22.632024994017197	23.142077600689507	31.626200533923836	22.59969687136946
24	23.338805322924358	24.23991425550253	29.72291979926575	22.698360622307366
25	24.99677618442984	23.77628459304052	26.904075442682355	24.322863779847285
26	22.164436785924853	23.537332384082088	28.62370365127852	25.67452717871454
27	21.13467512036078	22.582723107530292	31.83546365065475	24.447138121454177
28	19.818878544022926	23.80291480909914	30.11043742528995	26.26776922158799
29	22.479141163536518	24.67919286454157	28.968277055009988	23.873388916911928
30	25.649756279542895	21.745070860965683	29.89979481538483	22.705378044106595
31	25.063231768648198	22.52520423996224	29.95749361684517	22.454070374544393
32	27.563353224025494	21.887098679944987	28.227729132316785	22.32181896371273
33	26.676818936722647	21.17749938672532	28.145679277433473	24.000002399118564
34	22.77273329778639	21.413932521191715	32.356912070505295	23.456422110516595
35	22.5228051213984	24.240514035143487	29.9945000206924	23.242180822765715
36	24.2298979354985	24.294074357081207	28.350324090928993	23.1257036164913
37	22.091083735835454	24.78121538146885	30.536101036479195	22.5915998462165
38	23.667664500062678	23.468057835551217	30.39587255642277	22.468405107963335
39	22.503132349174912	21.606041940191172	29.950836062830515	25.939989647803397
40	24.62395315960916	20.70931139899201	30.84348810247115	23.82324733892768
41	23.012825088062648	21.618157488938564	29.380025778528967	25.988991644469824
42	22.27725533638941	21.806188406379494	31.374413040648864	24.542143216582225
43	21.436064389943134	20.740080094573255	32.52131167009241	25.302543845391202
44	22.372680277266134	20.935848169382567	30.169275808068114	26.522195745283184
45	22.51302871325075	20.45572456679416	28.56894377005888	28.462302949896205
46	22.43859605980763	21.22998010530931	30.294029973387776	26.03739386149529
47	20.239324072335823	22.24522710356215	32.6022219436579	24.913226880444125
48	20.16759042727702	23.29286220242683	32.07291641051078	24.466630959785373
49	23.5895132128456	20.346984517888128	31.80151612297642	24.261986146289853
50	21.328823790139502	21.024975424029215	30.411526805051825	27.234673980779462
51	20.296003248406535	21.112603229573455	29.754288274487955	28.837105247532058
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	1085.0
2	1845.0
3	9657.0
4	17469.0
5	11247.5
6	5026.0
7	4897.0
8	4768.0
9	4820.5
10	4873.0
11	4681.5
12	4490.0
13	4511.5
14	4533.0
15	4312.5
16	4092.0
17	4007.5
18	3923.0
19	3936.5
20	3950.0
21	4226.5
22	4503.0
23	5468.5
24	6434.0
25	7342.5
26	9311.5
27	10372.0
28	13269.0
29	16166.0
30	18536.0
31	20906.0
32	23797.0
33	26688.0
34	29777.0
35	32866.0
36	37172.0
37	41478.0
38	45462.0
39	49446.0
40	55394.5
41	61343.0
42	67996.5
43	74650.0
44	85397.5
45	96145.0
46	113564.0
47	130983.0
48	150918.0
49	170853.0
50	172574.0
51	174295.0
52	157805.5
53	141316.0
54	128283.5
55	115251.0
56	107395.0
57	99539.0
58	93501.5
59	87464.0
60	78152.0
61	68840.0
62	61595.5
63	54351.0
64	46554.0
65	38757.0
66	33382.0
67	28007.0
68	24066.0
69	20125.0
70	16401.5
71	12678.0
72	10763.5
73	8849.0
74	7029.0
75	4063.5
76	2918.0
77	2348.5
78	1779.0
79	1301.5
80	824.0
81	628.0
82	432.0
83	282.5
84	133.0
85	101.5
86	70.0
87	52.5
88	35.0
89	25.5
90	16.0
91	13.5
92	11.0
93	6.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1667279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.016421699775776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86266720044894	26.2425254806179
2	10.586088564438814	7.625460597760046
3	3.7696136339161233	4.073039828530418
4	1.9333067777889643	2.785231687355282
5	1.2125888866237147	2.1836556344550653
6	0.9234441484275451	1.9955492319574077
7	0.7700196361301557	1.941334635238011
8	0.6660290284828788	1.9190385883305068
9	0.5830533504641218	1.8899545809404668
>10	6.542532416219624	42.0279785216121
>50	0.10149933928388023	2.553420644832683
>100	0.0454943434792187	2.7832310820839252
>500	0.0021204956450866404	0.5167652257445255
>1k	0.0013494063196005895	0.9331351113806828
>5k	1.927723313715128E-4	0.5296791491609546
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7629	0.45757188808831634	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	3201	0.19198946307126763	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	2628	0.1576220896442647	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	2015	0.12085559765342213	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1942	0.11647720627441478	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.799338922879734E-4	0.0	0.0	0.20176587121891418	0.0
2	1.799338922879734E-4	0.0	0.0	0.6321677415717465	0.0
3	1.799338922879734E-4	0.0	0.0	0.9435733311581325	0.0
4	1.799338922879734E-4	0.0	0.0	1.3125577662766699	0.0
5	1.799338922879734E-4	0.0	0.0	1.9982258518220406	0.0
6	1.799338922879734E-4	0.0	0.0	2.740333201581739	0.0
7	1.799338922879734E-4	0.0	0.0	3.6387431257755902	0.0
8	1.799338922879734E-4	0.0	0.0	4.990586458535134	0.0
9	1.799338922879734E-4	0.0	0.0	5.880599467755546	0.0
10	1.799338922879734E-4	0.0	0.0	7.108228436872293	0.0
11	1.799338922879734E-4	0.0	0.0	9.028003111656778	0.0
12	1.799338922879734E-4	0.0	0.0	10.596186960910561	0.0
13	1.799338922879734E-4	0.0	0.0	11.128311458370195	0.0
14	1.799338922879734E-4	0.0	0.0	11.408528506626665	0.0
15	1.799338922879734E-4	0.0	0.0	11.67986881619693	0.0
16	2.3991185638396454E-4	0.0	0.0	12.225728267434544	0.0
17	2.3991185638396454E-4	0.0	0.0	12.922432298373577	0.0
18	2.3991185638396454E-4	0.0	0.0	13.648105685970974	0.0
19	2.3991185638396454E-4	0.0	0.0	14.254063057232774	0.0
20	3.598677845759468E-4	0.0	0.0	14.932773698943008	0.0
21	3.598677845759468E-4	0.0	0.0	15.846058158232665	0.0
22	3.598677845759468E-4	0.0	0.0	16.815242080059786	0.0
23	3.598677845759468E-4	0.0	0.0	17.668068751540684	0.0
24	3.598677845759468E-4	0.0	0.0	18.37071060092522	0.0
25	3.598677845759468E-4	0.0	0.0	19.01451406753159	0.0
26	3.598677845759468E-4	0.0	0.0	19.580166246920882	0.0
27	3.598677845759468E-4	0.0	0.0	20.18180520476777	0.0
28	4.1984574867193794E-4	0.0	0.0	20.758733241407107	0.0
29	4.1984574867193794E-4	0.0	0.0	21.413752587299427	0.0
30	4.1984574867193794E-4	0.0	0.0	22.1921466053372	0.0
31	4.798237127679291E-4	0.0	0.0	22.809079944028564	0.0
32	4.798237127679291E-4	0.0	0.0	23.423074362479227	0.0
33	4.798237127679291E-4	0.0	0.0	24.045345739975133	0.0
34	4.798237127679291E-4	0.0	0.0	24.7280749052798	0.0
35	4.798237127679291E-4	0.0	0.0	25.456807169046094	0.0
36	4.798237127679291E-4	0.0	0.0	26.090294425827953	0.0
37	4.798237127679291E-4	0.0	0.0	26.738956107526096	0.0
38	4.798237127679291E-4	0.0	0.0	27.585485092776914	0.0
39	5.398016768639202E-4	0.0	0.0	29.14677147615966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	1310	0.0	43.110687	1
TTTAGGG	5990	0.0	41.281303	3
GTTAGGG	2440	0.0	40.942623	3
CGTTTTT	3640	0.0	40.858517	1
CGTTAGG	665	0.0	40.26316	2
TTAGGGG	6370	0.0	40.16091	4
TCGTTAG	370	0.0	40.135136	1
TTAGGGA	2485	0.0	40.020123	4
TCGTTTG	360	0.0	40.0	1
CTTTAGG	1660	0.0	39.8494	2
TAGGGGG	5505	0.0	39.68665	5
TTTTAGG	3480	0.0	39.63362	2
TGTTAGG	1620	0.0	39.444443	2
TTAGGGT	1110	0.0	39.324326	4
TAGGGAT	1285	0.0	39.221786	5
CTTAGGG	3025	0.0	39.198345	3
TAGGGGA	3015	0.0	39.02985	5
TGTAGGG	2085	0.0	38.956837	3
AGGGGGG	6485	0.0	38.68543	6
TAGGGCA	890	0.0	38.679775	5
>>END_MODULE