FastQCFastQC Report
Sat 14 Jan 2017
SRR2934196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956728
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC563292.8787342952111894No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG423872.166218299119755No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC372601.9041992550829754No Hit
GAATCAGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC128690.6576795548487067No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTC122970.6284470810455005No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT115600.5907821628759847No Hit
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT99810.5100862255765748No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTA99420.5080931023627198No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC95690.4890306675225171TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGT92110.47073481853379723No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT69230.3538049233209726TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67410.34450368165631606No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC50070.2558863572249183TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC48040.24551189536818604TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGT37620.19225973155185594No Hit
GAACTATCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT33960.17355503677567857No Hit
GCTGTATCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC31340.16016533723644777TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATG29110.14876876091107194No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTAT25060.1280709429210396No Hit
CGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG21100.10783307644189688No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA207.034503E-445.015
CCCGTCG351.2125201E-745.040
CGTTTAT14000.043.3928571
TACGGCT66250.043.1320767
ACGGCTG66500.043.0037578
CGTTTTT34000.042.154411
TGATACC49250.041.6649784
TTAGGGA31700.041.6640364
TAGGGCG8050.041.6459665
TAATACG3150.041.428574
AATACGG3150.041.428575
TAGGGCA12500.041.45
GAATGAT187900.041.240021
CTAGGGT8150.041.134974
TTTAGGG68050.041.0653953
AGGGCGA10400.040.8894276
ATCAGTC15500.040.790323
GATACCT50400.040.7142835
CCGATGA7800.040.67307718
CGGCTGT72100.040.6310659
Produced by FastQC (version 0.11.5)