##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934195.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1354209
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34566599394923	33.0	31.0	34.0	31.0	34.0
2	32.55710233796999	34.0	31.0	34.0	31.0	34.0
3	32.49687751299836	34.0	31.0	34.0	31.0	34.0
4	36.052687583674306	37.0	35.0	37.0	35.0	37.0
5	35.17074986209662	37.0	35.0	37.0	35.0	37.0
6	26.023740796287722	37.0	0.0	37.0	0.0	37.0
7	31.04686425802812	37.0	19.0	37.0	17.0	37.0
8	34.76385476687867	37.0	32.0	37.0	32.0	37.0
9	37.295677402823344	39.0	37.0	39.0	35.0	39.0
10	37.495039539687006	39.0	37.0	39.0	35.0	39.0
11	37.52699398689567	39.0	37.0	39.0	35.0	39.0
12	37.145446530040786	39.0	37.0	39.0	34.0	39.0
13	37.11480281108751	39.0	37.0	39.0	34.0	39.0
14	38.053979112529895	40.0	37.0	41.0	33.0	41.0
15	38.25225796018192	40.0	38.0	41.0	33.0	41.0
16	38.40086796055853	40.0	38.0	41.0	34.0	41.0
17	38.386994917328124	40.0	37.0	41.0	34.0	41.0
18	38.286081395117	40.0	37.0	41.0	34.0	41.0
19	38.18367327347551	40.0	37.0	41.0	34.0	41.0
20	37.995598168377256	40.0	35.0	41.0	34.0	41.0
21	37.851138930549126	40.0	35.0	41.0	33.0	41.0
22	37.841600521042174	39.0	35.0	41.0	34.0	41.0
23	37.77464778331853	39.0	35.0	41.0	34.0	41.0
24	37.71015109189202	39.0	35.0	41.0	34.0	41.0
25	37.59220474830695	39.0	35.0	41.0	34.0	41.0
26	37.542017517237	39.0	35.0	41.0	33.0	41.0
27	37.510059377836065	39.0	35.0	41.0	33.0	41.0
28	37.393015406041464	39.0	35.0	41.0	33.0	41.0
29	37.300545927548846	39.0	35.0	41.0	33.0	41.0
30	36.97011465733871	39.0	35.0	41.0	33.0	41.0
31	36.860401164074375	39.0	35.0	41.0	32.0	41.0
32	36.625893787443445	39.0	35.0	41.0	31.0	41.0
33	36.300471345265024	39.0	35.0	41.0	30.0	41.0
34	35.92469995399529	39.0	35.0	41.0	29.0	41.0
35	35.74190394540282	39.0	35.0	41.0	28.0	41.0
36	35.619585307733146	39.0	35.0	41.0	27.0	41.0
37	35.533268498437096	39.0	35.0	41.0	27.0	41.0
38	35.433749148026635	38.0	35.0	41.0	26.0	41.0
39	35.35248990370024	38.0	35.0	41.0	25.0	41.0
40	35.22491579955531	38.0	35.0	40.0	24.0	41.0
41	35.06372650011926	38.0	35.0	40.0	23.0	41.0
42	34.995598906815715	38.0	34.0	40.0	23.0	41.0
43	34.900089277209055	38.0	34.0	40.0	23.0	41.0
44	34.73874121350545	38.0	34.0	40.0	23.0	41.0
45	34.68519482590944	38.0	34.0	40.0	23.0	41.0
46	34.633966396619726	37.0	34.0	40.0	23.0	41.0
47	34.56406138195803	37.0	34.0	40.0	23.0	41.0
48	34.50362979421936	37.0	34.0	40.0	23.0	41.0
49	34.464072384690986	37.0	34.0	40.0	23.0	41.0
50	34.34360944285557	37.0	34.0	40.0	23.0	41.0
51	33.34512398012419	35.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	9.0
10	12.0
11	19.0
12	11.0
13	17.0
14	19.0
15	46.0
16	121.0
17	223.0
18	406.0
19	719.0
20	1347.0
21	2171.0
22	3485.0
23	5717.0
24	10188.0
25	16742.0
26	23086.0
27	25568.0
28	24919.0
29	24141.0
30	26358.0
31	31784.0
32	42425.0
33	67855.0
34	98984.0
35	109576.0
36	126061.0
37	195797.0
38	258176.0
39	258068.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.732982132004736	4.052550234121912	50.367336208812674	23.84713142506068
2	27.653412434860495	3.74883049809889	48.201274692458846	20.39648237458177
3	19.5847908262314	3.8949674680939204	54.62738764843535	21.89285405723932
4	17.88328094112504	4.340245855698788	54.148879530412216	23.627593672763954
5	17.31091729563162	7.626001599457691	52.30610636910551	22.756974735805183
6	15.670107051422638	30.727014810860066	40.82818826340691	12.774689874310392
7	77.60648467112536	1.8401147828732491	15.973605255909536	4.579795290091854
8	79.21472977952443	1.9632863169569839	14.631197990856656	4.19078591266193
9	73.74784837495541	3.724978936043107	16.940590411081303	5.586582277920173
10	42.19511168512394	20.67590748547676	23.020818795326274	14.108162034073027
11	31.84530600520304	19.742373592259394	30.505778650119737	17.90654175241783
12	28.328197493887576	17.491022434498664	34.37179933082707	19.808980740786687
13	25.456114971913497	17.06287581902055	37.24550641739938	20.235502791666573
14	19.797461100908354	18.252943231066993	40.83498189718131	21.11461377084335
15	18.736472730575564	19.356761031716673	41.526160289881396	20.380605947826368
16	24.798240153477046	19.87292951088052	36.258066517059035	19.070763818583394
17	24.400443358447625	20.862880101963583	34.951695048548636	19.784981491040156
18	24.322464257732744	20.786525565846926	35.19109679525096	19.699913381169377
19	23.506046703278447	24.166062993230735	32.66416040655468	19.663729896936143
20	23.186007477427783	27.198755878893138	31.084271334779196	18.530965308899884
21	23.445125530844944	24.899701597020844	33.610986191939354	18.04418668019486
22	22.00709048603281	21.20758317216914	35.28716763808245	21.4981587037156
23	21.441372786623038	22.564980737832936	34.308145936114734	21.685500539429288
24	21.765621111660018	23.419206341118688	33.393073004240854	21.42209954298044
25	23.835981004409216	22.99246275870268	30.83445760587915	22.33709863100895
26	20.5930546909672	22.798622664596085	32.11845438924124	24.489868255195468
27	20.681224242343685	21.574808615213755	34.490171014961504	23.25379612748106
28	20.016703477823587	23.48573964580061	31.952231893304507	24.5453249830713
29	22.521708244443804	24.348604978995116	30.746140366811918	22.38354640974916
30	24.714870452049865	22.330452684925294	32.00303645892178	20.95164040410306
31	23.61851087978296	23.501173009483765	31.7309218887188	21.149394222014475
32	26.174763275092694	23.53440273990204	29.035769220260683	21.255064764744585
33	26.087332162169947	23.62995667581592	29.005567087502744	21.277144074511394
34	22.169251570473982	25.852656421571556	31.35114299196062	20.62694901599384
35	22.669469779037062	28.186934217687227	28.682057200919502	20.46153880235621
36	24.353995579707416	27.97773460374285	26.51444496381282	21.15382485273691
37	24.079001099534857	27.762110575250944	26.44392409148071	21.714964233733493
38	24.8312483523592	25.522426745059295	27.056680320393678	22.58964458218783
39	24.004197284171056	23.482490516604155	26.63687805944282	25.876434139781967
40	25.538746234886933	22.110767244937822	28.363199476594826	23.987287043580423
41	21.858147449913563	22.45207349825618	28.434458787380677	27.25532026444958
42	21.23113935884343	22.931024679351562	30.303224982259014	25.53461097954599
43	21.31133377491953	21.949861505868	31.802771950267644	24.936032768944823
44	23.30770213460404	22.47762346875556	29.917833953252416	24.296840443387985
45	22.456208753597117	22.099986043513226	29.040790601746114	26.40301460114355
46	21.966033308004896	23.063279006416295	29.940208638400716	25.030479047178094
47	20.556649675197846	23.795809952525794	31.765628496044556	23.881911876231808
48	21.21075845752022	24.10787404307607	30.620162766603976	24.061204732799737
49	23.7427900715473	22.096441538935274	29.823018455792273	24.33774993372515
50	21.275150290686298	22.605299477407108	30.2137262416658	25.905823990240794
51	20.532133518533698	22.436270915346153	30.134934858651803	26.896660707468346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	343.0
1	947.0
2	1551.0
3	20210.0
4	38869.0
5	25392.5
6	11916.0
7	11528.0
8	11140.0
9	11095.0
10	11050.0
11	10732.5
12	10415.0
13	9860.0
14	9305.0
15	8805.5
16	8306.0
17	7765.5
18	7225.0
19	6771.0
20	6317.0
21	6221.5
22	6126.0
23	6391.0
24	6656.0
25	7289.0
26	8750.5
27	9579.0
28	10746.0
29	11913.0
30	13771.0
31	15629.0
32	18406.5
33	21184.0
34	23923.0
35	26662.0
36	28328.0
37	29994.0
38	32603.5
39	35213.0
40	38107.5
41	41002.0
42	45701.0
43	50400.0
44	59278.5
45	68157.0
46	83659.0
47	99161.0
48	113670.0
49	128179.0
50	129058.0
51	129937.0
52	117066.5
53	104196.0
54	94401.5
55	84607.0
56	79307.5
57	74008.0
58	70646.0
59	67284.0
60	61236.0
61	55188.0
62	50609.5
63	46031.0
64	41217.5
65	36404.0
66	31725.5
67	27047.0
68	23224.5
69	19402.0
70	16294.5
71	13187.0
72	11001.5
73	8816.0
74	7571.5
75	5062.0
76	3797.0
77	2922.5
78	2048.0
79	1484.5
80	921.0
81	636.5
82	352.0
83	294.0
84	236.0
85	159.5
86	83.0
87	75.5
88	68.0
89	51.0
90	34.0
91	23.5
92	13.0
93	8.0
94	3.0
95	4.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1354209.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.30709626022236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96599929974633	27.56808462638478
2	8.597697946369692	6.587056856957998
3	3.950324915137502	4.539764303499811
4	2.690493850700037	4.122600277052106
5	2.1206104824126926	4.061721494010979
6	1.7469795137813522	4.015302743943523
7	1.4974785290027408	4.015483791068691
8	1.241263553906431	3.8039361955039483
9	1.075045319801315	3.7063678094757453
>10	4.9605568767050325	27.92060923924501
>50	0.09585306239984655	2.560247007113829
>100	0.05285350235210344	3.409098445376447
>500	0.0023162880232833597	0.5828771986820057
>1k	0.002105716384803054	1.2448856638886512
>5k	2.1057163848030544E-4	0.5198787082072415
>10k+	2.1057163848030544E-4	1.3420856395891518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16638	1.2286138993316393	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6445	0.4759235834350532	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	2138	0.15787814140948703	No Hit
CGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	1960	0.1447339369329254	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	1819	0.13432195473519967	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	1771	0.13077745015725048	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	1577	0.11645174415470581	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	1572	0.1160825249278361	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1386	0.10234756968828297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12605144405331822	0.0
2	0.0	0.0	0.0	0.3709914791586823	0.0
3	0.0	0.0	0.0	0.6491612446823201	0.0
4	0.0	0.0	0.0	0.9502226022718797	0.0
5	0.0	0.0	0.0	1.6589019863255967	0.0
6	0.0	0.0	0.0	2.781771499081752	0.0
7	0.0	0.0	0.0	4.165974380616286	0.0
8	0.0	0.0	0.0	6.118627183839422	0.0
9	0.0	0.0	0.0	7.765861842595936	0.0
10	0.0	0.0	0.0	9.539517164632638	0.0
11	0.0	0.0	0.0	11.289985519221922	0.0
12	0.0	0.0	0.0	12.461148906852635	0.0
13	0.0	0.0	0.0	12.98019729598607	0.0
14	0.0	0.0	0.0	13.415211389083959	0.0
15	0.0	0.0	0.0	13.671597220222285	0.0
16	0.0	0.0	0.0	13.95980974871678	0.0
17	0.0	0.0	0.0	14.327256723297511	0.0
18	0.0	0.0	0.0	14.748978924228092	0.0
19	0.0	0.0	0.0	15.060378420170004	0.0
20	0.0	0.0	0.0	15.445917136867353	0.0
21	0.0	0.0	0.0	15.915120930373376	0.0
22	0.0	0.0	0.0	16.44989805857146	0.0
23	0.0	0.0	0.0	16.946128699484348	0.0
24	0.0	0.0	0.0	17.341857866843302	0.0
25	0.0	0.0	0.0	17.69313303928714	0.0
26	0.0	0.0	0.0	18.02092586890207	0.0
27	0.0	0.0	0.0	18.390588158844018	0.0
28	7.384384537394154E-5	0.0	0.0	18.726282279913956	0.0
29	7.384384537394154E-5	0.0	0.0	19.140472408616397	0.0
30	7.384384537394154E-5	0.0	0.0	19.667865152277084	0.0
31	7.384384537394154E-5	0.0	0.0	20.055102277418037	0.0
32	7.384384537394154E-5	0.0	0.0	20.47763676064773	0.0
33	7.384384537394154E-5	0.0	0.0	20.85534802973544	0.0
34	1.4768769074788307E-4	0.0	0.0	21.310373804929668	0.0
35	1.4768769074788307E-4	0.0	0.0	21.80054925052189	0.0
36	1.4768769074788307E-4	0.0	0.0	22.234086466712302	0.0
37	1.4768769074788307E-4	0.0	0.0	22.67316197130576	0.0
38	2.2153153612182463E-4	0.0	0.0	23.279419941825818	0.0
39	2.2153153612182463E-4	0.0	0.0	24.448958764858304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAG	60	0.0	45.000004	1
CGTTTAT	3595	0.0	44.18637	1
TCGTTAG	225	0.0	44.0	1
CGTTTTT	8600	0.0	43.639534	1
CGTTAGG	375	0.0	42.6	2
GTTTATT	3810	0.0	41.811028	2
CACGACC	465	0.0	40.64516	27
TTTAGGG	4430	0.0	40.174946	3
TTAGGGA	2225	0.0	40.146065	4
CGTTATT	400	0.0	39.9375	1
TCAAGCG	485	0.0	39.896908	17
TAGGGAC	910	0.0	39.80769	5
ACGACCA	475	0.0	39.789474	28
GTTAGGG	1660	0.0	39.713856	3
TTGTTAG	720	0.0	39.6875	1
AGGGCGA	760	0.0	39.671055	6
TTAGGGG	4570	0.0	39.28884	4
TAGGGAT	1225	0.0	39.122448	5
TGTAGGG	1785	0.0	38.949577	3
CTTAGGG	2245	0.0	38.886414	3
>>END_MODULE