FastQCFastQC Report
Sat 14 Jan 2017
SRR2934193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254283
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC160311.2781007157076991No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG146721.1697519618778218No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133851.0671435393766797No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC117280.9350361919917595No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60310.480832475605585No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT59160.4716638908444107No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT47960.3823698479529739No Hit
GAATCAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC44190.3523128353011242No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC41350.3296704172822242No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC40040.3192262033368865No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT39840.31763166685668226No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC39770.3170735790886108No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT39550.31531958896038614No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT39210.31260887694403894No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC38530.3071874529113446No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTG34960.2787249767396991No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32550.2595108121532381No Hit
TCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC26620.21223280551518278No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT25520.20346285487405952No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC25470.20306422075400846No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA24570.1958888065930894No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC24450.1949320847049669No Hit
CGTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT21430.17085458385388305No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC19230.15331468257163655No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC17440.1390435810738087No Hit
GCTGTATCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC17150.13673150317751256No Hit
CGTTTATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT16820.1341005179851756No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG15540.12389548451186853No Hit
CGTTTACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT15000.11959023601531711No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGT14710.11727815811902098No Hit
CTGTCACTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT13840.11034192443013259No Hit
CGTTCAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC13570.10818930018185689No Hit
CCTGTATCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC13260.10571776863754034No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACT207.0335326E-445.00000413
GATCGAA800.045.00000442
GGCGTAA351.2120472E-745.0000048
CGCGCCA207.0335326E-445.00000441
CCCGTAG207.0335326E-445.0000041
TATTACG207.0335326E-445.0000041
CGATCGA800.045.00000441
TCGCAAG253.891011E-545.01
GCGAACC253.891011E-545.033
ATCGGGC253.891011E-545.026
GACACGA600.044.99999625
AGACACG600.044.99999624
GCGATAG302.1656615E-644.9999969
CGTTATT22650.044.701991
CGTTTAT29800.044.5469781
CGTTTTT55700.044.2324941
AATACGG6300.042.55
TAGGGCG4000.042.18755
ATCCGGC1450.041.8965536
GTTTATT29200.041.840752
Produced by FastQC (version 0.11.5)