##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1254283
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669777075827383	31.0	31.0	34.0	30.0	34.0
2	31.80417098852492	33.0	31.0	34.0	30.0	34.0
3	31.86354355436532	33.0	31.0	34.0	30.0	34.0
4	35.551401079341744	37.0	35.0	37.0	33.0	37.0
5	29.337799364258306	35.0	35.0	37.0	0.0	37.0
6	23.558927291528306	35.0	10.0	37.0	0.0	37.0
7	30.316632689751835	35.0	19.0	37.0	17.0	37.0
8	33.881895871984234	35.0	32.0	37.0	30.0	37.0
9	36.72159871416578	37.0	35.0	39.0	34.0	39.0
10	36.90974126253804	39.0	37.0	39.0	33.0	39.0
11	36.990893602161556	39.0	37.0	39.0	33.0	39.0
12	37.07877807480449	39.0	37.0	39.0	34.0	39.0
13	37.02246861354256	39.0	37.0	39.0	33.0	39.0
14	38.01678648279535	40.0	37.0	41.0	33.0	41.0
15	37.83316205353975	40.0	37.0	41.0	33.0	41.0
16	38.16026207801588	40.0	37.0	41.0	33.0	41.0
17	38.097037909307545	40.0	37.0	41.0	33.0	41.0
18	38.11627200560001	40.0	37.0	41.0	33.0	41.0
19	38.06918135699838	40.0	37.0	41.0	34.0	41.0
20	37.764690265275064	40.0	36.0	41.0	33.0	41.0
21	37.73588974736961	39.0	36.0	41.0	33.0	41.0
22	37.80934286759846	39.0	36.0	41.0	33.0	41.0
23	37.61750019732389	39.0	35.0	41.0	33.0	41.0
24	37.60167761183082	39.0	35.0	41.0	33.0	41.0
25	37.443570549867935	39.0	35.0	41.0	33.0	41.0
26	37.3325804463586	39.0	35.0	41.0	33.0	41.0
27	36.41190783898052	39.0	35.0	40.0	31.0	41.0
28	36.78661753368259	39.0	35.0	40.0	31.0	41.0
29	37.091248147347926	39.0	35.0	40.0	31.0	41.0
30	37.07366997719016	39.0	35.0	40.0	32.0	41.0
31	37.03529506498932	39.0	35.0	40.0	32.0	41.0
32	36.73139793810488	39.0	35.0	40.0	31.0	41.0
33	35.36531309122423	39.0	35.0	40.0	25.0	41.0
34	35.657410648155164	39.0	35.0	40.0	25.0	41.0
35	35.771807478854456	39.0	35.0	40.0	27.0	41.0
36	35.809294234235814	39.0	35.0	40.0	28.0	41.0
37	35.87940680053863	39.0	35.0	40.0	29.0	41.0
38	35.829684369476425	39.0	35.0	40.0	28.0	41.0
39	35.79743965277373	39.0	35.0	40.0	28.0	41.0
40	35.79177506192781	39.0	35.0	40.0	27.0	41.0
41	35.55351543471449	39.0	35.0	40.0	26.0	41.0
42	35.5380819161226	39.0	35.0	40.0	26.0	41.0
43	35.52325751046614	39.0	35.0	40.0	26.0	41.0
44	35.69107370505699	39.0	35.0	40.0	27.0	41.0
45	35.64333647191264	39.0	35.0	40.0	27.0	41.0
46	35.571400553144706	39.0	35.0	40.0	26.0	41.0
47	35.43660083091296	39.0	35.0	40.0	26.0	41.0
48	35.436168711526825	39.0	35.0	40.0	26.0	41.0
49	35.47449578763325	39.0	35.0	40.0	27.0	41.0
50	35.44346132411904	39.0	35.0	40.0	26.0	41.0
51	34.863109840442704	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	5.0
12	10.0
13	11.0
14	20.0
15	42.0
16	57.0
17	146.0
18	340.0
19	779.0
20	1662.0
21	3249.0
22	5795.0
23	8794.0
24	12592.0
25	16806.0
26	18914.0
27	18113.0
28	17092.0
29	18653.0
30	22787.0
31	30881.0
32	42974.0
33	63504.0
34	87064.0
35	112166.0
36	148229.0
37	208548.0
38	230046.0
39	184890.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.037279465638935	3.4850189311343613	49.080311221630204	20.397390381596498
2	24.936238472497834	12.581052282459382	44.151997595438985	18.3307116496038
3	15.279566094733008	12.22547064737384	53.68238268397164	18.812580573921515
4	14.720601331597413	3.3234126588656627	60.91759196289832	21.038394046638597
5	18.18465210801709	21.257323905370637	42.06251699177937	18.495506994832905
6	13.230028629902503	33.321268007299786	40.594746161751374	12.853957201046335
7	68.5165150129596	1.0467334724300656	24.61836762516912	5.818383889441218
8	68.77371374721653	7.298352923542772	17.30614223424857	6.621791094992119
9	64.7259031653941	3.403777297467956	18.183854839776988	13.686464697360961
10	37.717165902750814	25.339576475165494	23.50490280104251	13.438354821041184
11	23.79781915245602	19.724336533302292	36.48450947672894	19.993334837512748
12	19.819450634346474	17.61843220389657	38.34310119805499	24.21901596370197
13	21.916425559463057	19.354802704014965	41.84446412811144	16.88430760841054
14	17.106984627871064	26.464442235125567	36.80540994337004	19.623163193633335
15	13.305370478592152	23.40420782231761	42.58672085964651	20.70370083944373
16	16.03824655201418	25.82463447244362	34.24538162440215	23.891737351140055
17	16.50959153556255	27.321904227355386	34.509994953292036	21.65850928379002
18	16.395183543107894	24.95792416862861	35.402217840790314	23.24467444747318
19	16.904637948533146	27.135104278699462	32.68767893689064	23.272578835876754
20	18.311098850897288	29.229926579567767	34.57505204168437	17.883922527850572
21	17.540778277310622	30.758528976315553	34.36202196792909	17.338670778444737
22	16.369670959424628	23.91047315478245	36.57739122670083	23.142464659092088
23	17.137280820994942	27.461904530317323	33.558056674610114	21.84275797407762
24	18.322659240378766	26.717415447709968	31.91113967103118	23.048785640880087
25	17.6905052528018	30.91479355137557	28.855688867663837	22.539012328158798
26	16.455616475707636	25.577879952132015	32.66136908496727	25.305134487193083
27	19.59956405372631	26.806550036953382	31.631617426051378	21.962268483268925
28	16.148987110564363	25.062446034906	33.255892011611415	25.532674842918222
29	17.956872571819915	21.11843977794485	32.2298077866	28.694879863635244
30	19.16911893089518	24.843356722525936	33.2003224152763	22.787201931302583
31	21.22527372211853	22.9512797351156	29.599221228383065	26.2242253143828
32	20.94064896040208	26.666868641287493	27.094284144806235	25.298198253504196
33	19.42472312867192	25.90770982306226	26.850080882862958	27.817486165402865
34	17.938057041353506	23.90816107688616	30.628175619058855	27.52560626270148
35	15.753621790297723	26.76118547409157	29.698720304747813	27.78647243086289
36	18.323536235442877	28.830893825396657	28.328535107308316	24.51703483185214
37	16.945697262898403	26.829351908620303	33.69096129023514	22.533989538246153
38	19.62260510586526	27.30045771169664	26.660968856310735	26.41596832612736
39	17.99538062781685	26.114999565488812	29.630553870219085	26.259065936475263
40	18.198046214450805	25.081022384900375	31.599009155031197	25.12192224561762
41	17.25424007181792	28.41551707230346	29.78649953798305	24.543743317895565
42	17.93614359757726	28.346633096358637	31.320443631939522	22.396779674124577
43	19.740600805400376	26.869055866977387	29.33181746065282	24.058525866969415
44	20.08095461709997	23.04615465568775	30.815294475010823	26.05759625220146
45	20.19448561449051	21.021810867244472	28.120288642993646	30.66341487527137
46	22.448522382907207	23.582636454452462	28.70133773638007	25.267503426260262
47	15.865717704856081	24.16512063067107	35.15107834515815	24.8180833193147
48	17.9286492761203	25.359986542112107	31.024736841685645	25.68662734008194
49	19.377046487913812	21.968806082837762	34.29465280164046	24.359494627607965
50	19.01492725325943	21.746049336553234	31.55787011384193	27.6811532963454
51	16.92951271762433	21.28403239141406	29.920520329144217	31.86593456181739
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	279.0
1	547.0
2	815.0
3	15340.0
4	29865.0
5	18769.0
6	7673.0
7	7701.0
8	7729.0
9	8753.0
10	9777.0
11	10787.5
12	11798.0
13	11939.0
14	12080.0
15	11792.0
16	11504.0
17	10752.5
18	10001.0
19	9400.5
20	8800.0
21	8415.5
22	8031.0
23	7830.0
24	7629.0
25	8004.5
26	9347.0
27	10314.0
28	11350.0
29	12386.0
30	13892.0
31	15398.0
32	17568.5
33	19739.0
34	22181.0
35	24623.0
36	27450.0
37	30277.0
38	33653.0
39	37029.0
40	42582.0
41	48135.0
42	56844.5
43	65554.0
44	83372.0
45	101190.0
46	151786.0
47	202382.0
48	184478.0
49	166574.0
50	149259.5
51	131945.0
52	106813.5
53	81682.0
54	69238.5
55	56795.0
56	48739.5
57	40684.0
58	34508.0
59	28332.0
60	22989.0
61	17646.0
62	14768.0
63	11890.0
64	9611.5
65	7333.0
66	5818.0
67	4303.0
68	3245.5
69	2188.0
70	1798.5
71	1409.0
72	1181.0
73	953.0
74	818.0
75	470.5
76	258.0
77	188.5
78	119.0
79	97.5
80	76.0
81	48.5
82	21.0
83	12.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1254283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.125838047801345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.02476252376104	33.21757434270077
2	10.253027611694861	8.84340816563176
3	3.8924689216296313	5.035979529608983
4	2.132318093000089	3.6783201898047375
5	1.4044599359052277	3.028425587023716
6	1.0132919426033444	2.621943852711231
7	0.7517230937739507	2.269308187922131
8	0.578893063045973	1.9972198787133029
9	0.4540107462978191	1.7621634525121055
>10	2.3354541278190917	16.59807282346612
>50	0.09630629848514513	2.8361752377953184
>100	0.050277495440794025	3.996545147542998
>500	0.005047161345055894	1.477848479632142
>1k	0.006794255656806012	6.964129693262279
>5k	3.88243180388915E-4	1.0001617734189772
>10k+	7.7648636077783E-4	4.672723658253421
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	16031	1.2781007157076991	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	14672	1.1697519618778218	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13385	1.0671435393766797	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	11728	0.9350361919917595	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6031	0.480832475605585	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	5916	0.4716638908444107	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	4796	0.3823698479529739	No Hit
GAATCAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	4419	0.3523128353011242	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	4135	0.3296704172822242	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	4004	0.3192262033368865	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	3984	0.31763166685668226	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	3977	0.3170735790886108	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	3955	0.31531958896038614	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	3921	0.31260887694403894	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	3853	0.3071874529113446	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTG	3496	0.2787249767396991	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3255	0.2595108121532381	No Hit
TCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	2662	0.21223280551518278	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	2552	0.20346285487405952	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	2547	0.20306422075400846	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	2457	0.1958888065930894	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	2445	0.1949320847049669	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	2143	0.17085458385388305	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	1923	0.15331468257163655	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	1744	0.1390435810738087	No Hit
GCTGTATCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	1715	0.13673150317751256	No Hit
CGTTTATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	1682	0.1341005179851756	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	1554	0.12389548451186853	No Hit
CGTTTACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	1500	0.11959023601531711	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGT	1471	0.11727815811902098	No Hit
CTGTCACTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	1384	0.11034192443013259	No Hit
CGTTCAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	1357	0.10818930018185689	No Hit
CCTGTATCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	1326	0.10571776863754034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.972682401021141E-5	0.0	0.0	0.33501211449090834	0.0
2	7.972682401021141E-5	0.0	0.0	1.2533854002645337	0.0
3	7.972682401021141E-5	0.0	0.0	1.9017239331155729	0.0
4	7.972682401021141E-5	0.0	0.0	2.796577805806186	0.0
5	7.972682401021141E-5	0.0	0.0	5.075090709194018	0.0
6	7.972682401021141E-5	0.0	0.0	6.823260779265923	0.0
7	7.972682401021141E-5	0.0	0.0	9.083436513131407	0.0
8	1.5945364802042282E-4	0.0	0.0	11.636050237466346	0.0
9	1.5945364802042282E-4	0.0	0.0	12.841200909204701	0.0
10	1.5945364802042282E-4	0.0	0.0	15.762870101882909	0.0
11	1.5945364802042282E-4	0.0	0.0	18.46250008969268	0.0
12	1.5945364802042282E-4	0.0	0.0	22.070378056626772	0.0
13	1.5945364802042282E-4	0.0	0.0	22.899616753156984	0.0
14	2.3918047203063422E-4	0.0	0.0	23.29418480518352	0.0
15	2.3918047203063422E-4	0.0	0.0	23.86678285522486	0.0
16	2.3918047203063422E-4	0.0	0.0	24.605691060151496	0.0
17	2.3918047203063422E-4	0.0	0.0	25.650032727861255	0.0
18	2.3918047203063422E-4	0.0	0.0	26.686082805873955	0.0
19	2.3918047203063422E-4	0.0	0.0	28.079069874980366	0.0
20	2.3918047203063422E-4	0.0	0.0	28.866372262081207	0.0
21	2.3918047203063422E-4	0.0	0.0	29.784426640558788	0.0
22	2.3918047203063422E-4	0.0	0.0	30.831479020284895	0.0
23	2.3918047203063422E-4	0.0	0.0	31.796333044456475	0.0
24	2.3918047203063422E-4	0.0	0.0	32.558362028346075	0.0
25	2.3918047203063422E-4	0.0	0.0	33.15176877945408	0.0
26	2.3918047203063422E-4	0.0	0.0	33.705870206325045	0.0
27	2.3918047203063422E-4	0.0	0.0	34.334277033173535	0.0
28	2.3918047203063422E-4	0.0	0.0	34.91700038986417	0.0
29	2.3918047203063422E-4	0.0	0.0	35.559359410914446	0.0
30	2.3918047203063422E-4	0.0	0.0	36.25122878967506	0.0
31	2.3918047203063422E-4	0.0	0.0	36.83642367791001	0.0
32	2.3918047203063422E-4	0.0	0.0	37.361105906721214	0.0
33	2.3918047203063422E-4	0.0	0.0	37.87925053596357	0.0
34	2.3918047203063422E-4	0.0	0.0	38.438613933219216	0.0
35	2.3918047203063422E-4	0.0	0.0	39.050836214793634	0.0
36	2.3918047203063422E-4	0.0	0.0	39.61458458736983	0.0
37	2.3918047203063422E-4	0.0	0.0	40.18200039385051	0.0
38	2.3918047203063422E-4	0.0	0.0	40.802195357825944	0.0
39	2.3918047203063422E-4	0.0	0.0	41.66739085198476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACT	20	7.0335326E-4	45.000004	13
GATCGAA	80	0.0	45.000004	42
GGCGTAA	35	1.2120472E-7	45.000004	8
CGCGCCA	20	7.0335326E-4	45.000004	41
CCCGTAG	20	7.0335326E-4	45.000004	1
TATTACG	20	7.0335326E-4	45.000004	1
CGATCGA	80	0.0	45.000004	41
TCGCAAG	25	3.891011E-5	45.0	1
GCGAACC	25	3.891011E-5	45.0	33
ATCGGGC	25	3.891011E-5	45.0	26
GACACGA	60	0.0	44.999996	25
AGACACG	60	0.0	44.999996	24
GCGATAG	30	2.1656615E-6	44.999996	9
CGTTATT	2265	0.0	44.70199	1
CGTTTAT	2980	0.0	44.546978	1
CGTTTTT	5570	0.0	44.232494	1
AATACGG	630	0.0	42.5	5
TAGGGCG	400	0.0	42.1875	5
ATCCGGC	145	0.0	41.896553	6
GTTTATT	2920	0.0	41.84075	2
>>END_MODULE