SRR2934191.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1141379
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	9334	0.8177826996992235	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	8411	0.7369156082247877	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	7613	0.667000181359566	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	4010	0.35132940066358326	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4003	0.3507161074454673	No Hit
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	3665	0.32110280634215277	No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	3612	0.3164593005478461	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTATGCGT	3458	0.3029668497492945	No Hit
GAATCAGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	2637	0.2310363165959773	No Hit
TCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	2612	0.22884598367413456	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTC	2419	0.21193661351750823	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT	2325	0.2037009617313793	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTG	2053	0.17987013954172978	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT	1857	0.16269792943448233	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1803	0.1579668103233019	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTA	1690	0.1480665055165725	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCC	1553	0.136063481104874	No Hit
GAATATGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	1468	0.12861634917060855	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	1394	0.12213296372195388	No Hit
CCTGTATCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1312	0.11494867173830953	No Hit
CTGTCACTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	1260	0.11039277926087653	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTG	1249	0.10942903277526572	No Hit
GCTGTATCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1238	0.10846528628965489	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTT	1159	0.10154383425663167	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	30	2.1654887E-6	45.000004	41
CTAGGCG	30	2.1654887E-6	45.000004	4
TACGTCG	30	2.1654887E-6	45.000004	32
CAACGCG	25	3.8907885E-5	45.0	38
GCGCGTC	20	7.033268E-4	45.0	38
CGCGAAG	20	7.033268E-4	45.0	32
CTACGGT	35	1.2119199E-7	45.0	20
TGCGAAT	20	7.033268E-4	45.0	27
TACCTCG	20	7.033268E-4	45.0	36
GTCCGAC	20	7.033268E-4	45.0	19
CCGCGAA	20	7.033268E-4	45.0	31
CGGTAAG	20	7.033268E-4	45.0	37
CGTCCGA	20	7.033268E-4	45.0	18
TGGTCGA	20	7.033268E-4	45.0	24
CCGTCGA	145	0.0	43.44828	41
ACCCGTC	230	0.0	43.043476	39
TTGCGAG	105	0.0	42.857143	1
CCCGTCG	160	0.0	42.1875	40
TAGGGAG	1325	0.0	42.11321	5
CGTCGAA	150	0.0	42.0	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content