##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1141379
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.780348157798592	31.0	31.0	34.0	30.0	34.0
2	31.955395184246424	33.0	31.0	34.0	30.0	34.0
3	32.0121852601108	33.0	31.0	34.0	30.0	34.0
4	35.59307118844836	37.0	35.0	37.0	33.0	37.0
5	29.50827376357897	35.0	35.0	37.0	0.0	37.0
6	23.750208300660866	35.0	15.0	37.0	0.0	37.0
7	30.393461768614983	35.0	19.0	37.0	17.0	37.0
8	34.01901384202793	35.0	32.0	37.0	32.0	37.0
9	36.766060178082824	37.0	35.0	39.0	34.0	39.0
10	36.88727144971127	39.0	37.0	39.0	33.0	39.0
11	37.04890400121257	39.0	37.0	39.0	34.0	39.0
12	37.11459120940547	39.0	37.0	39.0	34.0	39.0
13	37.056653399090045	39.0	37.0	39.0	33.0	39.0
14	38.10401277752613	40.0	37.0	41.0	33.0	41.0
15	37.931669498037024	40.0	37.0	41.0	33.0	41.0
16	38.21019748917757	40.0	37.0	41.0	33.0	41.0
17	38.1438452959096	40.0	37.0	41.0	33.0	41.0
18	38.22008465198677	40.0	37.0	41.0	34.0	41.0
19	38.223074018358496	40.0	37.0	41.0	34.0	41.0
20	38.05949732735577	40.0	37.0	41.0	33.0	41.0
21	37.99733655516704	40.0	37.0	41.0	33.0	41.0
22	38.119147101882895	40.0	37.0	41.0	34.0	41.0
23	37.97459389037296	40.0	37.0	41.0	34.0	41.0
24	37.993243261002696	40.0	37.0	41.0	34.0	41.0
25	37.82149575206833	40.0	36.0	41.0	33.0	41.0
26	37.712067595426234	40.0	36.0	41.0	33.0	41.0
27	36.793229943778535	39.0	36.0	41.0	32.0	41.0
28	37.15804916684116	39.0	36.0	41.0	32.0	41.0
29	37.44643190386366	39.0	36.0	41.0	32.0	41.0
30	37.4325530783377	39.0	36.0	41.0	33.0	41.0
31	37.48989949876422	39.0	36.0	41.0	33.0	41.0
32	37.33287891226315	39.0	36.0	41.0	33.0	41.0
33	36.17377575721999	39.0	35.0	41.0	30.0	41.0
34	36.57333366042305	39.0	35.0	41.0	30.0	41.0
35	36.812135145293546	39.0	35.0	41.0	30.0	41.0
36	36.916637681261	39.0	35.0	41.0	31.0	41.0
37	36.945383610527266	39.0	35.0	41.0	31.0	41.0
38	36.91215801236925	39.0	35.0	41.0	31.0	41.0
39	36.97129262059316	39.0	35.0	41.0	32.0	41.0
40	36.9565700788257	39.0	35.0	41.0	31.0	41.0
41	36.846644278543764	39.0	35.0	41.0	31.0	41.0
42	36.86409597513184	39.0	35.0	41.0	31.0	41.0
43	36.712900798069704	39.0	35.0	41.0	31.0	41.0
44	36.76783697614903	39.0	35.0	40.0	31.0	41.0
45	36.67134229734383	39.0	35.0	40.0	31.0	41.0
46	36.6223024954901	39.0	35.0	40.0	31.0	41.0
47	36.46933752942712	39.0	35.0	40.0	31.0	41.0
48	36.448258641520475	39.0	35.0	40.0	31.0	41.0
49	36.46826777082809	39.0	35.0	40.0	31.0	41.0
50	36.34473036563666	39.0	35.0	40.0	31.0	41.0
51	35.626835608505154	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	8.0
11	8.0
12	14.0
13	19.0
14	14.0
15	31.0
16	56.0
17	104.0
18	180.0
19	557.0
20	1172.0
21	2199.0
22	3367.0
23	4663.0
24	6006.0
25	7535.0
26	8510.0
27	9476.0
28	10796.0
29	13854.0
30	18689.0
31	26169.0
32	37298.0
33	56572.0
34	78424.0
35	102247.0
36	137109.0
37	200379.0
38	227631.0
39	188190.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.781938339499852	4.141306261986597	50.11061181255306	16.96614358596049
2	20.888153715812187	9.976878845677026	46.684142602939076	22.45082483557171
3	19.39014122390547	10.471981699330371	48.96918552032234	21.168691556441814
4	17.66485978802834	4.124046438562476	53.76899347193176	24.442100301477424
5	17.832288836574005	21.86705730524217	39.8182374128138	20.48241644537003
6	15.314544949574154	32.138579735565486	38.71501052674002	13.831864788120335
7	76.7579392997418	1.8234083507756844	16.00800435262958	5.41064799685293
8	76.41195431140751	5.965590745931019	11.007123838795001	6.615331103866463
9	71.58402248508165	4.497279168444487	12.652589543000179	11.266108803473692
10	36.19174700077713	30.562941844908657	18.83519847482738	14.410112679486831
11	27.068397088083803	22.952761527941203	29.389536692019043	20.58930469195596
12	22.146631399386184	22.150311158694876	32.36900275894335	23.334054682975594
13	23.602939952461014	23.339924775206132	34.70854115942207	18.348594112910785
14	18.467835837175908	28.610654305011746	32.13673985591114	20.784770001901208
15	15.539010267404604	24.315586671911785	37.683626560502695	22.46177650018092
16	18.4631923313816	26.866974072591137	30.80633163918383	23.863501956843432
17	18.339569941272792	26.938816992427583	31.58153426688243	23.140078799417196
18	19.740857331350938	23.861486850555337	32.77631706908923	23.621338749004494
19	19.528132197981567	26.085112832810136	30.330416101925827	24.05633886728247
20	22.771927641913862	28.34203187547695	30.592379919378228	18.29366056323097
21	20.171038717200858	30.616035514934126	30.864068815003602	18.348856952861407
22	19.71904161544938	23.820396204941567	32.09161899772118	24.368943181887875
23	18.983790660245194	28.46179927964331	29.846352526198572	22.708057533912925
24	19.4796820337504	26.420146156535207	30.54857326094137	23.55159854877302
25	19.63239204506128	31.092301505459623	25.950538778092113	23.32476767138698
26	20.05749185853253	26.126553931691397	29.337231541845433	24.478722667930636
27	21.220208186763557	27.839306663255588	29.236301000806918	21.704184149173937
28	16.343826196206518	27.668811148619344	32.407990684952146	23.579371970221985
29	21.06872476188891	24.4928284119473	31.32395111527372	23.114495710890072
30	21.060138656835285	25.49249635747635	34.200909601455784	19.246455384232583
31	23.074719265029408	25.47804016019219	29.273974727062612	22.17326584771579
32	23.04291563100425	27.838255303453103	27.969500052129924	21.149329013412725
33	21.802048224121872	25.56959607632522	28.97889307583195	23.649462623720954
34	22.95696696715114	23.344655894317313	30.86687244114356	22.831504697387984
35	21.190156819075874	24.206332865770268	29.873512654429422	24.72999766072444
36	24.209837398445213	25.877031205235067	29.328733050108685	20.58439834621103
37	20.605425542260722	25.213973623134823	32.63490917565506	21.54569165894939
38	19.090766520147998	27.40737301106819	30.451410092528423	23.050450376255387
39	22.97659235013085	25.77101909181788	28.886461026530185	22.36592753152108
40	23.474323603290408	22.061909321969303	31.588017652331086	22.875749422409207
41	23.78841734428266	23.27990965314764	26.695865264736778	26.23580773783292
42	24.05537512079686	22.488410948510527	28.927025992242715	24.529187938449894
43	25.268644332864014	22.446531783044897	29.362989857006305	22.92183402708478
44	22.358480399586817	22.94049566357888	30.232376800344145	24.46864713649016
45	22.00969178511257	20.83838935182792	29.42756087154223	27.72435799151728
46	24.240764899301634	23.199480628257575	29.54837963551108	23.01137483692971
47	18.148572910488102	25.287744035942488	34.67586139222817	21.887821661341235
48	20.24603571644476	24.823393456511816	30.711796870277098	24.21877395676633
49	22.622809776594803	20.87615069140049	34.051441282869234	22.449598249135473
50	21.563477162274758	21.851900201423017	30.95466098465102	25.629961651651207
51	19.825842248718438	20.535860568662994	28.539599905027163	31.098697277591402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	569.0
2	900.0
3	5291.5
4	9683.0
5	6143.0
6	2603.0
7	2680.5
8	2758.0
9	3292.5
10	3827.0
11	4307.0
12	4787.0
13	4925.0
14	5063.0
15	4847.5
16	4632.0
17	4351.5
18	4071.0
19	3969.0
20	3867.0
21	4210.0
22	4553.0
23	5093.5
24	5634.0
25	6476.0
26	8999.0
27	10680.0
28	12269.5
29	13859.0
30	15419.5
31	16980.0
32	19423.5
33	21867.0
34	25057.0
35	28247.0
36	31256.0
37	34265.0
38	38042.0
39	41819.0
40	47518.5
41	53218.0
42	60178.5
43	67139.0
44	78966.0
45	90793.0
46	124550.5
47	158308.0
48	151846.0
49	145384.0
50	133575.0
51	121766.0
52	101584.5
53	81403.0
54	69519.0
55	57635.0
56	50866.0
57	44097.0
58	37002.5
59	29908.0
60	25348.0
61	20788.0
62	17702.0
63	14616.0
64	12532.0
65	10448.0
66	8435.5
67	6423.0
68	5205.0
69	3987.0
70	3395.0
71	2803.0
72	2475.0
73	2147.0
74	1871.0
75	1167.5
76	740.0
77	540.5
78	341.0
79	241.5
80	142.0
81	87.5
82	33.0
83	18.5
84	4.0
85	2.5
86	1.0
87	5.0
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1141379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.44550145987552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05268487283159	29.210452892589807
2	10.736966543803957	8.470500589565074
3	4.044624429454398	4.7862671651007505
4	2.244723110730643	3.5417691496536756
5	1.5184337213456593	2.9947689786032217
6	1.1599783488097446	2.745355659083927
7	0.9322471987283164	2.574107076688187
8	0.7105888054700599	2.242362541083236
9	0.5843342508951708	2.074442179206666
>10	3.848506939610373	26.35864148185175
>50	0.10280644104106128	2.800513260020331
>100	0.052429008104336934	3.943366137946695
>500	0.005494743141023136	1.4644595181456392
>1k	0.005494743141023136	4.502924027766386
>5k	6.86842892627892E-4	2.2900693426946916
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	9334	0.8177826996992235	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	8411	0.7369156082247877	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	7613	0.667000181359566	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	4010	0.35132940066358326	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4003	0.3507161074454673	No Hit
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	3665	0.32110280634215277	No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	3612	0.3164593005478461	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTATGCGT	3458	0.3029668497492945	No Hit
GAATCAGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	2637	0.2310363165959773	No Hit
TCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	2612	0.22884598367413456	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTC	2419	0.21193661351750823	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT	2325	0.2037009617313793	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTG	2053	0.17987013954172978	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT	1857	0.16269792943448233	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1803	0.1579668103233019	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTA	1690	0.1480665055165725	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCC	1553	0.136063481104874	No Hit
GAATATGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	1468	0.12861634917060855	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	1394	0.12213296372195388	No Hit
CCTGTATCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1312	0.11494867173830953	No Hit
CTGTCACTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	1260	0.11039277926087653	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTG	1249	0.10942903277526572	No Hit
GCTGTATCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1238	0.10846528628965489	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTT	1159	0.10154383425663167	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.761331687371153E-5	0.0	0.0	0.3423928423424647	0.0
2	8.761331687371153E-5	0.0	0.0	1.3109580603813458	0.0
3	8.761331687371153E-5	0.0	0.0	1.9282814910735173	0.0
4	8.761331687371153E-5	0.0	0.0	2.716188049718805	0.0
5	8.761331687371153E-5	0.0	0.0	4.509545032806806	0.0
6	8.761331687371153E-5	0.0	0.0	5.619956210864227	0.0
7	8.761331687371153E-5	0.0	0.0	7.11332519697664	0.0
8	8.761331687371153E-5	0.0	0.0	9.146216988397368	0.0
9	8.761331687371153E-5	0.0	0.0	10.011748945792764	0.0
10	8.761331687371153E-5	0.0	0.0	12.23222084864011	0.0
11	8.761331687371153E-5	0.0	0.0	14.907843932646387	0.0
12	8.761331687371153E-5	0.0	0.0	18.162941494455392	0.0
13	8.761331687371153E-5	0.0	0.0	18.988784619306998	0.0
14	8.761331687371153E-5	0.0	0.0	19.297095881385587	0.0
15	8.761331687371153E-5	0.0	0.0	19.830485754512743	0.0
16	8.761331687371153E-5	0.0	0.0	20.649144587380704	0.0
17	8.761331687371153E-5	0.0	0.0	21.818431914377257	0.0
18	8.761331687371153E-5	0.0	0.0	22.985178455184474	0.0
19	8.761331687371153E-5	0.0	0.0	24.198973347152872	0.0
20	8.761331687371153E-5	0.0	0.0	25.04067448235862	0.0
21	8.761331687371153E-5	0.0	0.0	26.114901360547197	0.0
22	8.761331687371153E-5	0.0	0.0	27.294264218984228	0.0
23	1.7522663374742306E-4	0.0	0.0	28.332832477205205	0.0
24	1.7522663374742306E-4	0.0	0.0	29.129587980854737	0.0
25	1.7522663374742306E-4	0.0	0.0	29.794660669243083	0.0
26	1.7522663374742306E-4	0.0	0.0	30.387802824478108	0.0
27	2.628399506211346E-4	0.0	0.0	31.017479732849473	0.0
28	2.628399506211346E-4	0.0	0.0	31.630510111014832	0.0
29	2.628399506211346E-4	0.0	0.0	32.315646248967255	0.0
30	2.628399506211346E-4	0.0	0.0	33.10215099454257	0.0
31	3.504532674948461E-4	0.0	0.0	33.75180373916114	0.0
32	3.504532674948461E-4	0.0	0.0	34.32978879057701	0.0
33	3.504532674948461E-4	0.0	0.0	34.91671040031401	0.0
34	3.504532674948461E-4	0.0	0.0	35.53639939056177	0.0
35	3.504532674948461E-4	0.0	0.0	36.20226059880198	0.0
36	3.504532674948461E-4	0.0	0.0	36.78427586279404	0.0
37	3.504532674948461E-4	0.0	0.0	37.36620351346923	0.0
38	3.504532674948461E-4	0.0	0.0	38.00140006080364	0.0
39	3.504532674948461E-4	0.0	0.0	38.84660572868434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	30	2.1654887E-6	45.000004	41
CTAGGCG	30	2.1654887E-6	45.000004	4
TACGTCG	30	2.1654887E-6	45.000004	32
CAACGCG	25	3.8907885E-5	45.0	38
GCGCGTC	20	7.033268E-4	45.0	38
CGCGAAG	20	7.033268E-4	45.0	32
CTACGGT	35	1.2119199E-7	45.0	20
TGCGAAT	20	7.033268E-4	45.0	27
TACCTCG	20	7.033268E-4	45.0	36
GTCCGAC	20	7.033268E-4	45.0	19
CCGCGAA	20	7.033268E-4	45.0	31
CGGTAAG	20	7.033268E-4	45.0	37
CGTCCGA	20	7.033268E-4	45.0	18
TGGTCGA	20	7.033268E-4	45.0	24
CCGTCGA	145	0.0	43.44828	41
ACCCGTC	230	0.0	43.043476	39
TTGCGAG	105	0.0	42.857143	1
CCCGTCG	160	0.0	42.1875	40
TAGGGAG	1325	0.0	42.11321	5
CGTCGAA	150	0.0	42.0	42
>>END_MODULE