FastQCFastQC Report
Sat 14 Jan 2017
SRR2934190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431352
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC42520.9857378660583468No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG36410.8440902093881563No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC25590.5932509875924998No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC21850.5065468573230216TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC19630.4550807693021013TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT18540.42981138374228006TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGT15390.35678517776665No Hit
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC15080.3495984717817467TruSeq Adapter, Index 19 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC10520.24388434503607262No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTC9770.22649715313711308No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT9720.2253380070105158No Hit
GCTGTATCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC8520.1975184999721805TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC8010.185695209480888TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTA7070.1639032623008587No Hit
CTGTCACTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT6700.15532558096403865TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC6160.14280680279678779TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG6140.14234314434614886TruSeq Adapter, Index 13 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC6120.14187948589550992No Hit
GAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT5820.13492460913592613No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC4750.11011888202674383No Hit
GCTGACTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC4530.10501863906971567TruSeq Adapter, Index 19 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG4450.10316400526716TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGCG302.1623364E-645.00000416
TAACGGG302.1623364E-645.0000043
TCGCTAG302.1623364E-645.0000041
AGGGCGC302.1623364E-645.0000046
GTGCATC207.028382E-445.018
CGTGGCA253.8867394E-545.015
TAATAGT207.028382E-445.023
AACCGAT207.028382E-445.040
CGTATGA207.028382E-445.015
GGTCGTA253.8867394E-545.08
GTACAAT502.1827873E-1145.07
TTAGCGC253.8867394E-545.020
TGAACGA207.028382E-445.028
CGCGCAT502.1827873E-1145.036
AATGGTC700.045.010
ATTCGAC502.1827873E-1145.016
CGTCTAA207.028382E-445.019
CACGAGT207.028382E-445.038
TCGGCAC207.028382E-445.026
CACTCGA351.2095734E-745.018
Produced by FastQC (version 0.11.5)