##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934190.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431352
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872477698028526	31.0	31.0	34.0	30.0	34.0
2	32.029602273781045	33.0	31.0	34.0	30.0	34.0
3	32.10619401324209	33.0	31.0	34.0	30.0	34.0
4	35.66377575622693	37.0	35.0	37.0	33.0	37.0
5	29.332883584636214	37.0	35.0	37.0	0.0	37.0
6	23.4926347855116	35.0	10.0	37.0	0.0	37.0
7	30.325330588475307	35.0	19.0	37.0	17.0	37.0
8	34.050017155362674	35.0	32.0	37.0	32.0	37.0
9	36.83140915076318	37.0	37.0	39.0	34.0	39.0
10	36.94982520076411	39.0	37.0	39.0	34.0	39.0
11	37.055064077597876	39.0	37.0	39.0	34.0	39.0
12	37.05654546634767	39.0	37.0	39.0	34.0	39.0
13	36.91983577217678	39.0	37.0	39.0	33.0	39.0
14	37.92891420464029	40.0	37.0	41.0	33.0	41.0
15	37.81262402863555	40.0	37.0	41.0	33.0	41.0
16	38.12307118084534	40.0	37.0	41.0	34.0	41.0
17	38.12080620931397	40.0	37.0	41.0	34.0	41.0
18	38.20675921289342	40.0	37.0	41.0	34.0	41.0
19	38.24961748177822	40.0	37.0	41.0	34.0	41.0
20	38.11475546653313	40.0	37.0	41.0	34.0	41.0
21	38.05843951111853	40.0	37.0	41.0	34.0	41.0
22	38.157071255030694	40.0	37.0	41.0	34.0	41.0
23	38.06066043509709	40.0	37.0	41.0	34.0	41.0
24	38.0599464010831	40.0	37.0	41.0	34.0	41.0
25	37.866658784473	40.0	36.0	41.0	34.0	41.0
26	37.77605065004915	40.0	36.0	41.0	33.0	41.0
27	36.89494658654649	39.0	36.0	41.0	32.0	41.0
28	37.279069066562805	39.0	36.0	41.0	32.0	41.0
29	37.619735158293	40.0	36.0	41.0	33.0	41.0
30	37.610719319720324	39.0	36.0	41.0	33.0	41.0
31	37.66954366735288	39.0	36.0	41.0	33.0	41.0
32	37.64235937239192	40.0	36.0	41.0	33.0	41.0
33	36.42883074611918	39.0	35.0	41.0	31.0	41.0
34	36.93888286132903	39.0	35.0	41.0	31.0	41.0
35	37.299697231031736	40.0	35.0	41.0	32.0	41.0
36	37.41170320295258	39.0	35.0	41.0	33.0	41.0
37	37.49360383167343	39.0	36.0	41.0	33.0	41.0
38	37.39465911830709	39.0	35.0	41.0	33.0	41.0
39	37.47941820137614	39.0	36.0	41.0	33.0	41.0
40	37.409612103340194	39.0	35.0	41.0	33.0	41.0
41	37.39098694337803	39.0	35.0	41.0	33.0	41.0
42	37.49294543667353	40.0	36.0	41.0	33.0	41.0
43	37.364247760529686	39.0	35.0	41.0	33.0	41.0
44	37.38224466329123	39.0	35.0	41.0	33.0	41.0
45	37.3562426973794	39.0	35.0	41.0	33.0	41.0
46	37.34313275468759	39.0	35.0	41.0	33.0	41.0
47	37.27904588364027	39.0	35.0	41.0	33.0	41.0
48	37.280309352918266	39.0	35.0	41.0	33.0	41.0
49	37.35879745544242	39.0	35.0	41.0	33.0	41.0
50	37.258401491125575	39.0	35.0	41.0	33.0	41.0
51	36.521330607021646	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	1.0
13	4.0
14	7.0
15	4.0
16	10.0
17	13.0
18	36.0
19	110.0
20	196.0
21	337.0
22	572.0
23	916.0
24	1190.0
25	1637.0
26	1983.0
27	2447.0
28	3256.0
29	4373.0
30	6591.0
31	9585.0
32	13996.0
33	23079.0
34	31529.0
35	40310.0
36	50154.0
37	75745.0
38	87291.0
39	75936.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.443711864092435	3.6935032177896474	52.51812904541998	16.344655872697935
2	17.43402140247408	9.27618279270758	52.411719430998346	20.87807637381999
3	18.09913944991561	10.07993471689015	51.16841929561008	20.652506537584152
4	18.16173334075187	4.255689089189339	53.75980637623101	23.82277119382778
5	17.741426955247686	22.483493759157255	39.6395519204733	20.135527365121757
6	15.506825052393406	32.18995159405775	38.544158830838846	13.759064522709991
7	78.48323411042489	1.3615330403011927	14.70075483595764	5.454478013316271
8	77.91548433761754	5.668920046736772	9.303538641295276	7.1120569743504145
9	73.3762681058625	5.021884678870157	10.820397262560508	10.781449952706838
10	39.405868061351285	28.6190860364621	17.397856043324246	14.577189858862369
11	27.119614607095826	23.45740833472431	29.15646618075261	20.26651087742725
12	24.148259426176303	19.880978875720988	33.54499341605	22.425768282052708
13	22.311708303195534	24.56601569020197	36.170923051243534	16.951352955358963
14	17.703870620745935	28.706717483632854	32.10139282998572	21.48801906563549
15	15.04803501548619	23.79193790686029	40.696693187930045	20.463333889723472
16	17.749772807359186	23.99687494204269	32.72849088447486	25.524861366123258
17	18.185148092509134	24.172137836384206	30.756783323132847	26.885930747973813
18	17.84273632671229	23.5895509931564	34.59077505146609	23.976937628665222
19	20.737587863276396	24.72018212503941	30.47279252211651	24.069437489567687
20	22.224308685250097	26.4783749698622	31.88880543036777	19.408510914519926
21	21.224197407221943	27.726311689756855	30.7859938055231	20.2634970974981
22	19.200328270183054	24.087288339917283	31.14602459244422	25.56635879745544
23	18.41860012240583	28.140126856952097	29.11241862794191	24.328854392700162
24	20.8901314935366	24.27391086629945	29.66927242715926	25.16668521300469
25	17.669096236948015	27.469676737328214	29.060720710695676	25.800506315028098
26	18.12185871399692	25.728639255179065	31.567258294849683	24.582243735974334
27	21.168558393145272	28.43524546078377	28.758183571653777	21.638012574417182
28	15.673510265398097	27.43884345036073	33.037519241825706	23.850127042415476
29	17.90231643761939	22.98818598267772	32.94270108867004	26.166796491032844
30	21.55293124872494	28.64319627589532	28.101179547098425	21.702692928281312
31	20.57693021013001	27.572145254919416	25.75669059144272	26.094233943507856
32	21.398996643112817	27.11103692575901	27.3658172443851	24.124149186743075
33	22.792522116508096	28.319330848124036	24.056918711400435	24.831228323967434
34	18.71951445687049	24.715777369758342	29.6173426806877	26.947365492683467
35	19.4201023757859	23.016700977392013	30.758869786160727	26.804326860661366
36	24.306830616294814	25.799810827352136	28.367783156215804	21.525575400137242
37	20.53914204640294	26.514308499786715	29.399423208887406	23.54712624492294
38	19.247389602922905	29.37229918952503	26.299170978690256	25.08114022886181
39	20.881553812199783	24.564161056399413	28.18510172666407	26.369183404736734
40	20.722982622081272	23.78706949312858	30.34690925276804	25.143038632022108
41	19.51468869971624	23.632207570615183	28.079155770693077	28.773947958975498
42	22.319822326081713	20.71301396539253	32.27758304122851	24.689580667297243
43	25.17224911441236	22.14177748103637	28.170032827018304	24.515940577532966
44	20.55954301823105	22.44663291233146	29.73256180567147	27.261262263766017
45	19.456267734935736	23.13029729779855	27.737671321797514	29.6757636454682
46	23.497051132253937	23.553849292457205	28.909799885012706	24.039299690276156
47	16.701441050464584	23.46575418683581	35.41469611825145	24.418108644448154
48	19.44143066451529	23.17828594743968	30.666601754483576	26.713681633561453
49	21.29560080862034	19.727507928559508	35.082716667593985	23.894174595226172
50	21.57008661139858	20.798327120310095	29.915938722899167	27.715647545392162
51	18.82244663291233	20.89662271184555	27.542007455627886	32.738923199614234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	204.5
2	308.0
3	609.5
4	911.0
5	684.0
6	457.0
7	492.0
8	527.0
9	680.0
10	833.0
11	853.5
12	874.0
13	898.5
14	923.0
15	957.0
16	991.0
17	962.5
18	934.0
19	964.5
20	995.0
21	1249.5
22	1504.0
23	1741.5
24	1979.0
25	2444.5
26	3267.0
27	3624.0
28	4256.0
29	4888.0
30	5798.0
31	6708.0
32	7637.5
33	8567.0
34	9594.5
35	10622.0
36	11708.0
37	12794.0
38	14371.0
39	15948.0
40	18923.5
41	21899.0
42	25327.0
43	28755.0
44	33434.5
45	38114.0
46	50608.5
47	63103.0
48	61159.0
49	59215.0
50	53178.0
51	47141.0
52	37988.5
53	28836.0
54	24211.0
55	19586.0
56	16462.5
57	13339.0
58	11830.5
59	10322.0
60	9126.5
61	7931.0
62	6977.0
63	6023.0
64	4992.0
65	3961.0
66	3119.5
67	2278.0
68	1719.0
69	1160.0
70	1025.0
71	890.0
72	769.5
73	649.0
74	507.0
75	301.5
76	238.0
77	162.5
78	87.0
79	61.5
80	36.0
81	28.0
82	20.0
83	11.0
84	2.0
85	3.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.822857466701684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99790583636627	27.231665303498907
2	11.59294311023994	8.76956469756373
3	4.519856799288795	5.128616984682078
4	2.3054358007035276	3.4879267875456317
5	1.5521475424361828	2.9353327632427524
6	1.1597209005734066	2.6318374994125726
7	0.9051257816032412	2.3964110398911447
8	0.7435696783419167	2.2499143968390025
9	0.6371302341777476	2.16882774315281
>10	4.406223865081974	29.777085110847008
>50	0.11536251922791244	2.949997343187861
>100	0.05266549790839481	3.643389001667066
>500	0.006269702131951763	1.7555329848568917
>1k	0.005642731918756587	4.873898343612549
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC	4252	0.9857378660583468	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG	3641	0.8440902093881563	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	2559	0.5932509875924998	No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2185	0.5065468573230216	TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1963	0.4550807693021013	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	1854	0.42981138374228006	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGT	1539	0.35678517776665	No Hit
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1508	0.3495984717817467	TruSeq Adapter, Index 19 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	1052	0.24388434503607262	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTC	977	0.22649715313711308	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	972	0.2253380070105158	No Hit
GCTGTATCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	852	0.1975184999721805	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	801	0.185695209480888	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTA	707	0.1639032623008587	No Hit
CTGTCACTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	670	0.15532558096403865	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	616	0.14280680279678779	TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	614	0.14234314434614886	TruSeq Adapter, Index 13 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	612	0.14187948589550992	No Hit
GAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	582	0.13492460913592613	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC	475	0.11011888202674383	No Hit
GCTGACTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	453	0.10501863906971567	TruSeq Adapter, Index 19 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	445	0.10316400526716	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46064467070976833	0.0
2	0.0	0.0	0.0	1.8875535525510487	0.0
3	0.0	0.0	0.0	2.569595133440902	0.0
4	0.0	0.0	0.0	3.3974572970566963	0.0
5	0.0	0.0	0.0	5.15379550807693	0.0
6	0.0	0.0	0.0	5.955229140006305	0.0
7	0.0	0.0	0.0	7.231912683840576	0.0
8	0.0	0.0	0.0	9.202461099055991	0.0
9	0.0	0.0	0.0	9.965874738032975	0.0
10	0.0	0.0	0.0	12.388953801071978	0.0
11	0.0	0.0	0.0	14.976631614087799	0.0
12	0.0	0.0	0.0	18.42856877909457	0.0
13	0.0	0.0	0.0	19.24831691982418	0.0
14	0.0	0.0	0.0	19.539494426825424	0.0
15	0.0	0.0	0.0	20.14178675420538	0.0
16	0.0	0.0	0.0	21.068871826257904	0.0
17	0.0	0.0	0.0	22.55396984365437	0.0
18	0.0	0.0	0.0	24.055759565273835	0.0
19	0.0	0.0	0.0	25.48915966542406	0.0
20	0.0	0.0	0.0	26.52033605964502	0.0
21	0.0	0.0	0.0	27.78079155770693	0.0
22	0.0	0.0	0.0	29.07463046421484	0.0
23	0.0	0.0	0.0	30.208970863702962	0.0
24	0.0	0.0	0.0	31.09154472449415	0.0
25	0.0	0.0	0.0	31.858435801850923	0.0
26	0.0	0.0	0.0	32.55299616090803	0.0
27	0.0	0.0	0.0	33.25937981045642	0.0
28	0.0	0.0	0.0	33.95788126634396	0.0
29	0.0	0.0	0.0	34.71851295461711	0.0
30	0.0	0.0	0.0	35.56723974851165	0.0
31	0.0	0.0	0.0	36.305847660379456	0.0
32	0.0	0.0	0.0	37.0866484912554	0.0
33	0.0	0.0	0.0	37.72881544539031	0.0
34	0.0	0.0	0.0	38.39091971290269	0.0
35	0.0	0.0	0.0	39.169865909976075	0.0
36	0.0	0.0	0.0	39.870221999666164	0.0
37	0.0	0.0	0.0	40.56315955414603	0.0
38	0.0	0.0	0.0	41.26119735158293	0.0
39	0.0	0.0	0.0	42.12615219124984	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAGCG	30	2.1623364E-6	45.000004	16
TAACGGG	30	2.1623364E-6	45.000004	3
TCGCTAG	30	2.1623364E-6	45.000004	1
AGGGCGC	30	2.1623364E-6	45.000004	6
GTGCATC	20	7.028382E-4	45.0	18
CGTGGCA	25	3.8867394E-5	45.0	15
TAATAGT	20	7.028382E-4	45.0	23
AACCGAT	20	7.028382E-4	45.0	40
CGTATGA	20	7.028382E-4	45.0	15
GGTCGTA	25	3.8867394E-5	45.0	8
GTACAAT	50	2.1827873E-11	45.0	7
TTAGCGC	25	3.8867394E-5	45.0	20
TGAACGA	20	7.028382E-4	45.0	28
CGCGCAT	50	2.1827873E-11	45.0	36
AATGGTC	70	0.0	45.0	10
ATTCGAC	50	2.1827873E-11	45.0	16
CGTCTAA	20	7.028382E-4	45.0	19
CACGAGT	20	7.028382E-4	45.0	38
TCGGCAC	20	7.028382E-4	45.0	26
CACTCGA	35	1.2095734E-7	45.0	18
>>END_MODULE