FastQCFastQC Report
Sat 14 Jan 2017
SRR2934188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068113
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98230.9196592495363318No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC73570.6887848008590851No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG65230.6107031746641038No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC48950.45828484439380474No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT44240.41418838643476863No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43580.40800926493732403No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC37970.3554867322090453No Hit
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC35750.3347024144449136TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT35540.3327363303320903TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT34780.3256209783047299No Hit
CGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG30990.2901378412209195TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC27200.25465470413710906TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAGAGTTT25650.24014313092341352No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.23583647048580064No Hit
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC23280.2179544673644081TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC19720.1846246604994041No Hit
GAATCAGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC19640.18387567607547142No Hit
CGTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT18710.1751687321472541No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC17540.1642148349472387No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT17330.16224875083441548No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC15720.14717543930277038No Hit
CGTTTATGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT15440.14455399381900605No Hit
CGTTCAGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC13020.12189721499504266No Hit
CTGTCACTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT12540.1174033084514466TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT12530.11730968539845504No Hit
CCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC12210.11431374770272434TruSeq Adapter, Index 15 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC12010.11244128664289266No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTA11860.11103694084801888No Hit
TCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG11650.1090708567351956TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT11640.10897723368220405No Hit
GCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC11410.1068239034633976TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT11400.10673028041040603No Hit
CGCTGACTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG11030.1032662274497174TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCA207.0330617E-445.00000422
ACCGGCG207.0330617E-445.00000420
CGCGAGG502.1827873E-1145.0000042
TACGAGG253.890619E-545.0000042
GGACGAT207.0330617E-445.0000048
CGTAAGG406.8139343E-945.0000042
TTACGCG207.0330617E-445.0000041
TCGCGAG253.890619E-545.0000041
ACGAACG253.890619E-545.00000421
GCGTAAG302.1653577E-644.9999961
CGTTATT19750.044.5443041
CGTTTAT22500.044.51
AGGGCGC1500.043.5000046
GTTATTT20050.043.428932
CGTTTTT49500.043.3636321
TAGGGCG4700.043.0851065
TCGTTAG1150.043.0434761
TCGCTAG800.042.1875041
CAACGGG750.042.0000043
TAATACG2800.041.7857134
Produced by FastQC (version 0.11.5)