##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934188.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1068113
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.743441939195574	31.0	31.0	34.0	30.0	34.0
2	31.9475018092655	33.0	31.0	34.0	30.0	34.0
3	31.97918665908944	33.0	31.0	34.0	30.0	34.0
4	35.580016346585055	37.0	35.0	37.0	33.0	37.0
5	29.29960032318678	35.0	35.0	37.0	0.0	37.0
6	23.5821678043428	35.0	10.0	37.0	0.0	37.0
7	30.363342642585568	35.0	19.0	37.0	17.0	37.0
8	34.10853533287209	35.0	32.0	37.0	32.0	37.0
9	36.8115012175678	37.0	37.0	39.0	34.0	39.0
10	36.91655751779072	39.0	37.0	39.0	33.0	39.0
11	37.056905964069344	39.0	37.0	39.0	34.0	39.0
12	37.20289332682965	39.0	37.0	39.0	34.0	39.0
13	37.21214422069575	39.0	37.0	39.0	34.0	39.0
14	38.30638144091496	40.0	38.0	41.0	34.0	41.0
15	38.133083297366476	40.0	38.0	41.0	33.0	41.0
16	38.36414592838024	40.0	38.0	41.0	34.0	41.0
17	38.3150359559335	40.0	38.0	41.0	34.0	41.0
18	38.32772749699704	40.0	38.0	41.0	34.0	41.0
19	38.26625928155541	40.0	37.0	41.0	34.0	41.0
20	38.06796378285818	40.0	37.0	41.0	34.0	41.0
21	37.952874836276685	39.0	37.0	41.0	34.0	41.0
22	38.011818037979126	39.0	37.0	41.0	34.0	41.0
23	37.854080982068375	39.0	36.0	41.0	34.0	41.0
24	37.830769778104	39.0	36.0	41.0	33.0	41.0
25	37.60926512457015	39.0	36.0	41.0	33.0	41.0
26	37.49851279780323	39.0	35.0	41.0	33.0	41.0
27	36.58397285680448	39.0	35.0	40.0	32.0	41.0
28	36.94872265387651	39.0	35.0	40.0	32.0	41.0
29	37.22073881696038	39.0	35.0	40.0	32.0	41.0
30	37.121170700103825	39.0	35.0	40.0	32.0	41.0
31	37.043884869859276	39.0	35.0	40.0	32.0	41.0
32	36.78569402301068	39.0	35.0	40.0	31.0	41.0
33	35.35432955127407	39.0	35.0	40.0	26.0	41.0
34	35.62820132326823	39.0	35.0	40.0	25.0	41.0
35	35.7371242555797	39.0	35.0	40.0	27.0	41.0
36	35.783191478804206	39.0	35.0	40.0	28.0	41.0
37	35.92797016795039	39.0	35.0	40.0	29.0	41.0
38	35.771470808800196	39.0	35.0	40.0	28.0	41.0
39	35.81591835320795	39.0	35.0	40.0	28.0	41.0
40	35.72113624682033	39.0	35.0	40.0	27.0	41.0
41	35.59427981870832	39.0	35.0	40.0	26.0	41.0
42	35.64398055261943	39.0	35.0	40.0	27.0	41.0
43	35.654158314710145	39.0	35.0	40.0	27.0	41.0
44	35.79931336852936	39.0	35.0	40.0	28.0	41.0
45	35.73048825358366	39.0	35.0	40.0	27.0	41.0
46	35.6756691473655	39.0	35.0	40.0	27.0	41.0
47	35.583881106212544	39.0	35.0	40.0	27.0	41.0
48	35.5262364562551	39.0	35.0	40.0	26.0	41.0
49	35.569183223123396	39.0	35.0	40.0	27.0	41.0
50	35.51415159257494	39.0	35.0	40.0	27.0	41.0
51	34.86026291225741	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	10.0
12	4.0
13	16.0
14	19.0
15	32.0
16	34.0
17	93.0
18	221.0
19	596.0
20	1152.0
21	2143.0
22	3740.0
23	6136.0
24	9360.0
25	13303.0
26	15699.0
27	15189.0
28	14342.0
29	15580.0
30	19285.0
31	25249.0
32	34974.0
33	51006.0
34	71527.0
35	96523.0
36	133216.0
37	186549.0
38	203422.0
39	148598.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.504242528646316	3.6042066710170175	50.89854725108673	20.993003549249938
2	25.94247986870303	7.840837064992187	46.27506640215033	19.94161666415445
3	16.304454678484394	8.027802301816381	55.32467070431687	20.34307231538236
4	15.48693817976188	3.6574781881692298	58.56955209795218	22.28603153411671
5	15.356802136103578	21.603144985596092	44.08138464750452	18.958668230795805
6	13.515798422077067	30.99044763990327	42.39270564069532	13.101048297324347
7	71.31333482506065	1.039871249577526	21.60997946846448	6.036814456897351
8	72.04162855428218	4.356187032645422	16.91253640766473	6.689648005407668
9	67.17528950588562	3.758216593188174	18.702702803916814	10.363791097009399
10	33.830222083243996	25.28880371271579	25.533159881023828	15.347814323016385
11	21.97230068354191	21.790016599367295	35.40505545761544	20.832627259475355
12	18.23028087852128	18.99190441460782	39.54272628457851	23.2350884222924
13	19.96726938067414	20.429673639399574	41.984696375758	17.618360604168284
14	17.04725998091962	25.858312744063593	37.33902686326259	19.755400411754188
15	14.080439054669307	24.429063217094072	40.91758081775992	20.572916910476703
16	15.682984852726257	25.855316806367867	36.01407341732569	22.447624923580182
17	15.95982822042237	26.84481885343592	34.9460216287977	22.249331297344007
18	16.477750949571814	25.09322515501637	35.91885877243325	22.510165122978563
19	16.758526485493576	27.7595160811637	33.29226402075436	22.18969341258837
20	17.896327448500298	28.688069520734228	34.092179385514456	19.32342364525102
21	17.7249972615257	30.008809929286507	33.84838495552437	18.417807853663422
22	16.749632295459378	24.986869366817928	34.70044836080078	23.563049976921917
23	16.26943965666554	27.28990284735791	33.075620276131836	23.365037219844716
24	18.16652357943401	26.203407317390575	32.81244587417249	22.817623229002923
25	18.024310161939795	29.002830224891934	29.625329904232977	23.34752970893529
26	16.818632485514172	26.436622342392614	31.742428001531675	25.002317170561543
27	18.517048289834502	27.369014327135798	30.366637237820342	23.747300145209355
28	16.31063379998184	25.962421578990234	31.88304982712503	25.843894793902894
29	18.301059906582918	23.391532543841333	30.32918801662371	27.978219532952036
30	19.45880257987685	26.732283943740036	29.20870731842043	24.60020615796269
31	19.99544991962461	27.71935179143031	26.84406986901199	25.441128419933097
32	20.68048979836403	28.474421713807434	27.17905315261587	23.66603533521266
33	19.820093941371372	29.360657533425773	26.545880445233788	24.273368079969067
34	20.712789751646127	26.749604208543477	28.531719022238285	24.00588701757211
35	19.58416384783258	24.612377154851593	29.770445636369935	26.033013360945894
36	18.82347654227596	27.450747252397452	29.725132078722005	24.00064412660458
37	19.439797100119556	27.108180501501245	31.81648383644802	21.63553856193118
38	19.43240087893322	28.964725642324364	30.811721231742332	20.791152247000085
39	22.81912119785079	26.136654080607574	30.607810222326666	20.43641449921497
40	21.641155945110675	24.907851510093032	32.21999919484174	21.230993349954545
41	20.273510387009612	24.23638697403739	31.453694506105627	24.036408132847367
42	18.5860484798893	23.945032033127582	34.28757069710789	23.181348789875226
43	19.27633124959625	24.053541151544827	33.146867419458424	23.52326017940049
44	20.57123169552285	24.555173469473736	29.38293982003777	25.490655014965647
45	20.834499720535188	23.07134170261012	27.8173751279125	28.2767834489422
46	23.363071135731893	23.751325936487994	28.335765972326897	24.549836955453216
47	17.705242797344475	24.895680513204127	33.228881213879056	24.170195475572342
48	18.530342763359307	24.77986879665354	31.59272473979813	25.097063700189025
49	20.566831412032247	21.60239600117216	34.068305507001604	23.762467079793993
50	20.21714930910868	21.12192249321935	32.43149367154973	26.229434526122237
51	18.433630149619002	21.155252300084353	30.637114237913032	29.774003312383613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	431.0
2	617.0
3	11792.5
4	22968.0
5	14805.0
6	6642.0
7	6772.5
8	6903.0
9	7678.5
10	8454.0
11	9294.5
12	10135.0
13	10196.5
14	10258.0
15	9896.5
16	9535.0
17	8997.0
18	8459.0
19	8011.0
20	7563.0
21	7189.5
22	6816.0
23	6676.0
24	6536.0
25	6859.5
26	7750.0
27	8317.0
28	9338.5
29	10360.0
30	12247.5
31	14135.0
32	15821.5
33	17508.0
34	20282.5
35	23057.0
36	25336.5
37	27616.0
38	30536.0
39	33456.0
40	38553.0
41	43650.0
42	51413.5
43	59177.0
44	73628.0
45	88079.0
46	121600.5
47	155122.0
48	151454.5
49	147787.0
50	132617.5
51	117448.0
52	94397.5
53	71347.0
54	58673.5
55	46000.0
56	39304.5
57	32609.0
58	27224.5
59	21840.0
60	17786.0
61	13732.0
62	11682.0
63	9632.0
64	7946.0
65	6260.0
66	4708.0
67	3156.0
68	2442.0
69	1728.0
70	1492.0
71	1256.0
72	1207.0
73	1158.0
74	977.0
75	523.5
76	251.0
77	211.5
78	172.0
79	120.5
80	69.0
81	53.0
82	37.0
83	30.5
84	24.0
85	21.0
86	18.0
87	9.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1068113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.95532520964476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32472594722701	38.34412430702799
2	10.07137782157055	9.860951531283797
3	3.862796973622906	5.673134461876229
4	2.1542457238960755	4.218471999792758
5	1.356734937778654	3.3209700051120583
6	0.9248729185408274	2.71664727028557
7	0.661704793832217	2.267578136238599
8	0.4927130717245678	1.929674292905539
9	0.3668545620034799	1.6163535948769931
>10	1.65019262872389	13.353554195376171
>50	0.07920840939415486	2.6510342792474884
>100	0.043933585862084	3.9318820036127153
>500	0.004137243375824222	1.3818056857774506
>1k	0.005910347679748888	6.447719250756902
>5k	5.910347679748888E-4	2.2860989858297556
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9823	0.9196592495363318	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	7357	0.6887848008590851	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	6523	0.6107031746641038	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	4895	0.45828484439380474	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	4424	0.41418838643476863	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4358	0.40800926493732403	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	3797	0.3554867322090453	No Hit
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3575	0.3347024144449136	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	3554	0.3327363303320903	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	3478	0.3256209783047299	No Hit
CGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	3099	0.2901378412209195	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	2720	0.25465470413710906	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAGAGTTT	2565	0.24014313092341352	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2519	0.23583647048580064	No Hit
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	2328	0.2179544673644081	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC	1972	0.1846246604994041	No Hit
GAATCAGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	1964	0.18387567607547142	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	1871	0.1751687321472541	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC	1754	0.1642148349472387	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	1733	0.16224875083441548	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC	1572	0.14717543930277038	No Hit
CGTTTATGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	1544	0.14455399381900605	No Hit
CGTTCAGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	1302	0.12189721499504266	No Hit
CTGTCACTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	1254	0.1174033084514466	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	1253	0.11730968539845504	No Hit
CCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1221	0.11431374770272434	TruSeq Adapter, Index 15 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	1201	0.11244128664289266	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTA	1186	0.11103694084801888	No Hit
TCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	1165	0.1090708567351956	TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	1164	0.10897723368220405	No Hit
GCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1141	0.1068239034633976	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGT	1140	0.10673028041040603	No Hit
CGCTGACTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	1103	0.1032662274497174	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.362305299158422E-5	0.0	0.0	0.359606146540675	0.0
2	9.362305299158422E-5	0.0	0.0	1.2504294957555988	0.0
3	9.362305299158422E-5	0.0	0.0	1.9607475988027483	0.0
4	9.362305299158422E-5	0.0	0.0	2.8110321660723163	0.0
5	9.362305299158422E-5	0.0	0.0	4.728245045233978	0.0
6	9.362305299158422E-5	0.0	0.0	6.658284282655487	0.0
7	9.362305299158422E-5	0.0	0.0	8.924804772528748	0.0
8	9.362305299158422E-5	0.0	0.0	11.642494754768457	0.0
9	9.362305299158422E-5	0.0	0.0	12.967448200705356	0.0
10	9.362305299158422E-5	0.0	0.0	15.420933927402812	0.0
11	9.362305299158422E-5	0.0	0.0	18.279807473553827	0.0
12	9.362305299158422E-5	0.0	0.0	21.379105019787232	0.0
13	9.362305299158422E-5	0.0	0.0	22.276388359658576	0.0
14	9.362305299158422E-5	0.0	0.0	22.638990443894983	0.0
15	9.362305299158422E-5	0.0	0.0	23.13303929453157	0.0
16	9.362305299158422E-5	0.0	0.0	23.924060469257466	0.0
17	9.362305299158422E-5	0.0	0.0	25.086203426042	0.0
18	9.362305299158422E-5	0.0	0.0	26.316129473192444	0.0
19	9.362305299158422E-5	0.0	0.0	27.4991503707941	0.0
20	9.362305299158422E-5	0.0	0.0	28.309083402224296	0.0
21	9.362305299158422E-5	0.0	0.0	29.359159564577904	0.0
22	9.362305299158422E-5	0.0	0.0	30.503888633506005	0.0
23	9.362305299158422E-5	0.0	0.0	31.6019934220443	0.0
24	9.362305299158422E-5	0.0	0.0	32.48223736627117	0.0
25	9.362305299158422E-5	0.0	0.0	33.16081725435417	0.0
26	9.362305299158422E-5	0.0	0.0	33.782006210953334	0.0
27	9.362305299158422E-5	0.0	0.0	34.43924004295425	0.0
28	9.362305299158422E-5	0.0	0.0	35.092541706729534	0.0
29	9.362305299158422E-5	0.0	0.0	35.79639981912026	0.0
30	9.362305299158422E-5	0.0	0.0	36.52815760130248	0.0
31	9.362305299158422E-5	0.0	0.0	37.1932557697547	0.0
32	9.362305299158422E-5	0.0	0.0	37.80433343663077	0.0
33	9.362305299158422E-5	0.0	0.0	38.414474872976925	0.0
34	9.362305299158422E-5	0.0	0.0	39.03557020652309	0.0
35	9.362305299158422E-5	0.0	0.0	39.70450692014796	0.0
36	1.8724610598316844E-4	0.0	0.0	40.318112409454805	0.0
37	1.8724610598316844E-4	0.0	0.0	40.93724165888815	0.0
38	1.8724610598316844E-4	0.0	0.0	41.583427970636066	0.0
39	1.8724610598316844E-4	0.0	0.0	42.33372311731062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCA	20	7.0330617E-4	45.000004	22
ACCGGCG	20	7.0330617E-4	45.000004	20
CGCGAGG	50	2.1827873E-11	45.000004	2
TACGAGG	25	3.890619E-5	45.000004	2
GGACGAT	20	7.0330617E-4	45.000004	8
CGTAAGG	40	6.8139343E-9	45.000004	2
TTACGCG	20	7.0330617E-4	45.000004	1
TCGCGAG	25	3.890619E-5	45.000004	1
ACGAACG	25	3.890619E-5	45.000004	21
GCGTAAG	30	2.1653577E-6	44.999996	1
CGTTATT	1975	0.0	44.544304	1
CGTTTAT	2250	0.0	44.5	1
AGGGCGC	150	0.0	43.500004	6
GTTATTT	2005	0.0	43.42893	2
CGTTTTT	4950	0.0	43.363632	1
TAGGGCG	470	0.0	43.085106	5
TCGTTAG	115	0.0	43.043476	1
TCGCTAG	80	0.0	42.187504	1
CAACGGG	75	0.0	42.000004	3
TAATACG	280	0.0	41.785713	4
>>END_MODULE