##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934187.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1386596
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99381362704061	33.0	31.0	34.0	30.0	34.0
2	32.223678706703325	33.0	31.0	34.0	30.0	34.0
3	32.28154199204383	34.0	31.0	34.0	30.0	34.0
4	35.71773249021344	37.0	35.0	37.0	35.0	37.0
5	29.630539825587267	37.0	35.0	37.0	0.0	37.0
6	23.73028409140081	35.0	10.0	37.0	0.0	37.0
7	30.425777948299288	35.0	19.0	37.0	17.0	37.0
8	34.36505442104261	35.0	32.0	37.0	32.0	37.0
9	36.98318616237174	38.0	37.0	39.0	34.0	39.0
10	36.996003882890186	39.0	37.0	39.0	34.0	39.0
11	37.181918886250934	39.0	37.0	39.0	34.0	39.0
12	37.19845001716433	39.0	37.0	39.0	34.0	39.0
13	37.17614359193305	39.0	37.0	39.0	34.0	39.0
14	38.224913385009046	40.0	38.0	41.0	33.0	41.0
15	38.10799252269587	40.0	38.0	41.0	33.0	41.0
16	38.34549284723164	40.0	38.0	41.0	34.0	41.0
17	38.36733338333588	40.0	38.0	41.0	34.0	41.0
18	38.40452157658034	40.0	38.0	41.0	34.0	41.0
19	38.384399637673845	40.0	37.0	41.0	34.0	41.0
20	38.32859895744687	40.0	37.0	41.0	34.0	41.0
21	38.1828672518888	40.0	37.0	41.0	34.0	41.0
22	38.26975990122573	40.0	37.0	41.0	34.0	41.0
23	38.21519750525748	40.0	37.0	41.0	34.0	41.0
24	38.203094484622774	40.0	37.0	41.0	34.0	41.0
25	37.986476955075595	40.0	36.0	41.0	34.0	41.0
26	37.87008256189979	40.0	36.0	41.0	34.0	41.0
27	37.0732268086739	40.0	36.0	41.0	33.0	41.0
28	37.43534598397803	40.0	36.0	41.0	33.0	41.0
29	37.70628503183335	40.0	36.0	41.0	33.0	41.0
30	37.70401328144607	40.0	36.0	41.0	33.0	41.0
31	37.69314710268889	40.0	36.0	41.0	33.0	41.0
32	37.65135410746894	40.0	36.0	41.0	33.0	41.0
33	36.40604617350692	40.0	35.0	41.0	31.0	41.0
34	36.79071409408364	40.0	35.0	41.0	31.0	41.0
35	37.049553727257255	40.0	35.0	41.0	31.0	41.0
36	37.15429944987581	40.0	35.0	41.0	32.0	41.0
37	37.24159740832946	40.0	36.0	41.0	33.0	41.0
38	37.174922616248715	39.0	35.0	41.0	33.0	41.0
39	37.1749355976795	39.0	35.0	41.0	33.0	41.0
40	37.158014302651964	39.0	35.0	41.0	33.0	41.0
41	37.078885270114725	39.0	35.0	41.0	32.0	41.0
42	37.091806120888855	39.0	35.0	41.0	33.0	41.0
43	36.97558264988504	39.0	35.0	41.0	32.0	41.0
44	37.00133925094259	39.0	35.0	41.0	32.0	41.0
45	36.94776993442935	39.0	35.0	41.0	32.0	41.0
46	36.89471482681329	39.0	35.0	41.0	32.0	41.0
47	36.83908651113951	39.0	35.0	41.0	32.0	41.0
48	36.77301102844664	39.0	35.0	41.0	32.0	41.0
49	36.78671148625843	39.0	35.0	40.0	32.0	41.0
50	36.703007941750876	39.0	35.0	40.0	32.0	41.0
51	35.979919890148246	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	9.0
11	11.0
12	16.0
13	16.0
14	20.0
15	32.0
16	50.0
17	80.0
18	221.0
19	357.0
20	671.0
21	1124.0
22	2024.0
23	3274.0
24	5400.0
25	8237.0
26	10423.0
27	11618.0
28	12856.0
29	15385.0
30	20367.0
31	28716.0
32	41104.0
33	66653.0
34	93555.0
35	117527.0
36	160699.0
37	245664.0
38	287600.0
39	252715.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.306857945645305	4.593262925899108	56.1392792132676	16.960599915187984
2	21.71447198751475	3.908276094839448	52.07883190201039	22.29842001563541
3	20.324377107679524	3.898107307391627	53.578187157614764	22.199328427314086
4	18.114000040386674	4.338610525344079	52.102559072721974	25.44483036154727
5	14.603676918150638	21.93227154845391	42.85321751973898	20.610834013656465
6	15.888694327691699	29.369116887687547	40.897060138641685	13.845128645979074
7	81.10610444570733	1.8897357269168524	11.71538068766966	5.2887791397061585
8	82.92408170801012	1.938199735178812	9.894158067670757	5.243560489140312
9	77.66790038338492	4.633721718510655	12.077057773136515	5.621320124967907
10	38.21704375319127	23.141419706965834	21.161967869516427	17.47956867032647
11	28.055684568540514	23.314721807938287	26.721698317318094	21.907895306203105
12	23.40256282291309	21.54246802962074	32.93013970904287	22.124829438423305
13	23.400471370175595	22.144301584599983	34.55570331949609	19.89952372572833
14	20.674010310140805	23.34594936088089	35.28006715726859	20.69997317170971
15	18.469835482000523	23.51182319868224	36.28836373392105	21.729977585396178
16	21.630741758955025	23.566561565156686	32.2811402888801	22.521556387008186
17	22.389290031126585	23.930474341480863	29.76497840755346	23.915257219839088
18	23.0100187798032	22.541893961903828	32.32520503448734	22.122882223805636
19	23.176036855724377	25.28487028665884	30.06845541167002	21.470637445946764
20	23.513481937060256	26.891178108115128	29.41664334817063	20.178696606653993
21	23.08386869715476	26.159097530931863	31.22279308464758	19.534240687265793
22	22.61040706882178	22.711301633640947	30.898762148455646	23.779529149081636
23	21.239207382683926	24.12389766016922	30.183485312232257	24.453409644914597
24	21.3739979056625	24.261645064604252	30.283370210212635	24.08098681952061
25	21.827915268758886	25.190827032531466	28.02445701559791	24.956800683111734
26	20.184754607686738	25.259772853808897	30.23057905835586	24.324893480148507
27	20.208986611817718	25.87985253094629	31.30378278893059	22.607378068305405
28	19.283771192185757	23.601250832975143	31.57812369284204	25.536854281997062
29	20.417843409327592	22.81948022351139	32.40193971423544	24.36073665292558
30	22.75334704557059	22.75969352284299	31.84590176230135	22.64105766928507
31	22.368952456230943	24.529855848423043	29.917870814570357	23.183320880775653
32	22.68065103317765	24.36924670199539	29.44830361547271	23.501798649354246
33	23.0195384957118	26.06231375252777	27.62059028008158	23.297557471678846
34	21.083646570450224	23.195004168481663	30.494751174819484	25.226598086248625
35	19.7818975390092	24.435884713355584	30.857726403364786	24.92449134427043
36	22.953333198711086	25.126857426388078	29.00671861162155	22.913090763279282
37	21.63723247434725	25.771962417315496	29.268943513467516	23.321861594869738
38	22.08227919307426	25.64899942016276	28.728555397534684	23.5401659892283
39	22.17697151874086	24.24570675236334	28.90156902226748	24.675752706628316
40	22.47381356934536	22.36152419305984	29.526552795479	25.638109442115802
41	19.504239158341726	23.982400064618677	27.71138817651284	28.80197260052676
42	20.340099062740695	24.58682990575481	29.726538948619496	25.34653208288499
43	20.880270821493788	23.575864923885543	31.16531419389642	24.37855006072425
44	21.732069038133677	22.166658493173212	30.060089600720037	26.041182867973077
45	22.43407596733295	20.776996327697468	29.72300511468373	27.06592259028585
46	22.023718516424395	21.53792452884618	30.801329298512325	25.6370276562171
47	18.740570432916293	23.76467262273943	32.44600445984266	25.048752484501613
48	19.37399213613771	23.671062082971535	32.162504435322184	24.792441345568573
49	22.71079680022155	21.339741352203525	31.944127921903714	24.005333925671213
50	21.125403506140216	21.51109623855831	30.307241619044046	27.056258636257425
51	20.28911088738176	21.242957573799433	28.272258105461145	30.195673433357662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	254.0
1	702.5
2	1151.0
3	5990.0
4	10829.0
5	7487.5
6	4146.0
7	4142.5
8	4139.0
9	4218.5
10	4298.0
11	4246.0
12	4194.0
13	4201.0
14	4208.0
15	4121.0
16	4034.0
17	4011.0
18	3988.0
19	4093.5
20	4199.0
21	4355.0
22	4511.0
23	5316.0
24	6121.0
25	7278.0
26	10008.5
27	11582.0
28	13476.0
29	15370.0
30	17486.5
31	19603.0
32	23591.0
33	27579.0
34	31205.5
35	34832.0
36	38216.5
37	41601.0
38	46510.0
39	51419.0
40	57343.5
41	63268.0
42	70817.0
43	78366.0
44	88967.0
45	99568.0
46	116667.5
47	133767.0
48	148371.5
49	162976.0
50	154121.5
51	145267.0
52	123256.0
53	101245.0
54	88321.5
55	75398.0
56	68193.0
57	60988.0
58	54796.5
59	48605.0
60	44949.0
61	41293.0
62	37550.5
63	33808.0
64	29620.0
65	25432.0
66	22017.5
67	18603.0
68	15295.5
69	11988.0
70	10078.0
71	8168.0
72	6619.0
73	5070.0
74	4128.5
75	2379.5
76	1572.0
77	1239.0
78	906.0
79	651.0
80	396.0
81	259.0
82	122.0
83	87.0
84	52.0
85	46.5
86	41.0
87	26.5
88	12.0
89	8.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1386596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.536516317301896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.76704294704547	32.55096585260457
2	10.674069407753906	9.29423593885382
3	3.9598445517112975	5.171935108186738
4	2.1158411567776314	3.6846541218747317
5	1.4280611976120063	3.1086404815970403
6	1.046924238420932	2.7347660513395065
7	0.799413740326016	2.436258254498526
8	0.6622064551967486	2.3064129713676715
9	0.5525538688055603	2.165064347289741
>10	3.8802157101124517	28.828456785888157
>50	0.07572051305372383	2.2337445668705205
>100	0.03389254864217498	2.6971695792886123
>500	0.0018320280617869116	0.5978020964450277
>1k	0.002381636480322985	2.189893843895221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	4249	0.3064338855730148	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	3727	0.2687877362980998	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3519	0.25378697183606475	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	2545	0.1835430074801889	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	2152	0.15520021693413222	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	1846	0.13313178460056138	No Hit
CTGTCACTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	1678	0.1210157825350715	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1653	0.11921280603723074	No Hit
CCTGTATCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	1403	0.10118304105882318	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.211905991363021E-5	0.0	0.0	0.32273279311349523	0.0
2	7.211905991363021E-5	0.0	0.0	0.979232595507271	0.0
3	7.211905991363021E-5	0.0	0.0	1.4387752452769227	0.0
4	7.211905991363021E-5	0.0	0.0	1.9386324495382938	0.0
5	1.4423811982726043E-4	0.0	0.0	2.8026909063634973	0.0
6	1.4423811982726043E-4	0.0	0.0	3.6970393683524256	0.0
7	1.4423811982726043E-4	0.0	0.0	4.698484634313095	0.0
8	1.4423811982726043E-4	0.0	0.0	6.192430960423945	0.0
9	1.4423811982726043E-4	0.0	0.0	6.85268095393323	0.0
10	1.4423811982726043E-4	0.0	0.0	8.07466630510978	0.0
11	1.4423811982726043E-4	0.0	0.0	10.436565517281169	0.0
12	1.4423811982726043E-4	0.0	0.0	12.265576995750745	0.0
13	1.4423811982726043E-4	0.0	0.0	12.956405470663409	0.0
14	1.4423811982726043E-4	0.0	0.0	13.222452682684791	0.0
15	1.4423811982726043E-4	0.0	0.0	13.566388479412893	0.0
16	1.4423811982726043E-4	0.0	0.0	14.401815669452386	0.0
17	1.4423811982726043E-4	0.0	0.0	15.540142911129125	0.0
18	1.4423811982726043E-4	0.0	0.0	16.767320834619458	0.0
19	1.4423811982726043E-4	0.0	0.0	17.609815692530486	0.0
20	1.4423811982726043E-4	0.0	0.0	18.48786524697893	0.0
21	1.4423811982726043E-4	0.0	0.0	19.762209035652777	0.0
22	1.4423811982726043E-4	0.0	0.0	21.077660688477394	0.0
23	1.4423811982726043E-4	0.0	0.0	22.39830491361579	0.0
24	1.4423811982726043E-4	0.0	0.0	23.400904084535078	0.0
25	1.4423811982726043E-4	0.0	0.0	24.229407844822862	0.0
26	2.1635717974089063E-4	0.0	0.0	24.9920669034095	0.0
27	2.1635717974089063E-4	0.0	0.0	25.765399582863356	0.0
28	2.1635717974089063E-4	0.0	0.0	26.55791593225424	0.0
29	2.1635717974089063E-4	0.0	0.0	27.41295950659024	0.0
30	2.1635717974089063E-4	0.0	0.0	28.329664877152393	0.0
31	2.8847623965452086E-4	0.0	0.0	29.1762705214785	0.0
32	2.8847623965452086E-4	0.0	0.0	29.979027777377116	0.0
33	3.6059529956815106E-4	0.0	0.0	30.754235552388728	0.0
34	4.3271435948178126E-4	0.0	0.0	31.520500563971048	0.0
35	4.3271435948178126E-4	0.0	0.0	32.36003854042562	0.0
36	4.3271435948178126E-4	0.0	0.0	33.122913956192	0.0
37	4.3271435948178126E-4	0.0	0.0	33.93764297603628	0.0
38	4.3271435948178126E-4	0.0	0.0	34.77299804701586	0.0
39	5.048334193954115E-4	0.0	0.0	35.89372823807367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGT	20	7.033791E-4	45.0	27
TAACCGA	20	7.033791E-4	45.0	25
ATAACGG	20	7.033791E-4	45.0	23
TATAACG	20	7.033791E-4	45.0	22
CGTATCA	25	3.8912247E-5	44.999996	14
CGCGTAA	30	2.165827E-6	44.999996	13
TCGCGTA	30	2.165827E-6	44.999996	12
TCGTTAG	215	0.0	43.95349	1
CGTTATT	925	0.0	43.783783	1
CGTTTAT	1215	0.0	42.962963	1
CGTTTTT	2530	0.0	42.420948	1
TGCGTAG	75	0.0	42.0	1
TACCGGT	70	0.0	41.785713	40
AGGGCGC	135	0.0	41.666668	6
TTGTTCG	180	0.0	41.25	1
TACGGGT	290	0.0	41.120693	4
TTAATCG	105	0.0	40.714287	20
CGTTAGG	410	0.0	40.609756	2
TAGGGCG	670	0.0	39.96269	5
TTAGGGA	2430	0.0	39.629627	4
>>END_MODULE