FastQCFastQC Report
Sat 14 Jan 2017
SRR2934186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088606
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC153181.4071206662465574No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG136761.25628556153466No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC128391.1793982395834675No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63560.5838659717106097No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGAGAGGT60000.5511635982164347No Hit
GAATCAGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC45130.41456688645846157No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC40300.37019821680203857No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT37860.34778423047457024No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC37320.3428237580906223Illumina Single End Adapter 1 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT34480.3167353477750444Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT33870.3111318511931773Illumina Single End Adapter 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC31140.2860539074743295Illumina Single End Adapter 1 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.2644666665441859No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTA27200.24986083119145036No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT24820.22799800846219845No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC23000.21127937931629992No Hit
CGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTG22330.20512471913621638Illumina Single End Adapter 1 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC22220.20411425253948628No Hit
TCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC19900.18280259340845081Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT18780.17251420624174402No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.14302695373716476No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC14790.13586182696035112No Hit
GCTGTATCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC14240.13080949397670047Illumina Single End Adapter 1 (95% over 23bp)
CTGTCACTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT13210.12134785220731835Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC13000.11941877961356084No Hit
GAACTATCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT12420.11409086483080197Illumina Single End Adapter 1 (95% over 21bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG12350.1134478406328828No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT11950.10977341664477323No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGATCACCGGTCGAATGC11560.1061908532563664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC351.2118471E-745.0000041
CCGCGTA351.2118471E-745.00000440
TCGTTAG1050.045.01
AGTCCCG207.033122E-445.012
CAACGCG253.89067E-545.015
GCCGATC207.033122E-445.09
CCCGTAT207.033122E-445.041
TACGATT207.033122E-445.044
GCGATAT253.89067E-545.09
ATGCGAT253.89067E-545.025
GTCGGTT207.033122E-445.027
CCGTCTA302.165396E-644.99999641
GCCGATA302.165396E-644.9999969
CGTTATT13600.044.3382381
CGTTTAT16100.044.3012431
ACCGGTC3200.044.29687539
CGGTCGA3100.043.54838641
CGTTTTT33950.043.4094241
CGATGAA25350.042.42603719
TAATACG8000.042.18754
Produced by FastQC (version 0.11.5)