##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1132305
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969496734537074	33.0	31.0	34.0	30.0	34.0
2	32.169877374029085	33.0	31.0	34.0	30.0	34.0
3	32.2522023659703	34.0	31.0	34.0	30.0	34.0
4	35.694289082888446	37.0	35.0	37.0	33.0	37.0
5	29.24449772808563	37.0	35.0	37.0	0.0	37.0
6	23.402324462048654	35.0	10.0	37.0	0.0	37.0
7	30.25909538507734	35.0	19.0	37.0	17.0	37.0
8	34.16601622354401	35.0	32.0	37.0	32.0	37.0
9	36.857886346876505	38.0	37.0	39.0	34.0	39.0
10	36.998433284318274	39.0	37.0	39.0	34.0	39.0
11	37.169021597537764	39.0	37.0	39.0	34.0	39.0
12	37.06447114514199	39.0	37.0	39.0	34.0	39.0
13	36.925611032363186	39.0	37.0	39.0	33.0	39.0
14	37.74381019248347	40.0	37.0	41.0	33.0	41.0
15	37.71021412075368	40.0	37.0	41.0	33.0	41.0
16	38.05303782991332	40.0	37.0	41.0	33.0	41.0
17	38.10503353778355	40.0	37.0	41.0	33.0	41.0
18	38.193652770234166	40.0	37.0	41.0	34.0	41.0
19	38.204841451728996	40.0	37.0	41.0	34.0	41.0
20	38.08714171535055	40.0	37.0	41.0	33.0	41.0
21	38.00573343754554	40.0	37.0	41.0	33.0	41.0
22	38.14490353747444	40.0	37.0	41.0	34.0	41.0
23	38.0942131316209	40.0	36.0	41.0	34.0	41.0
24	38.072172250409565	40.0	36.0	41.0	34.0	41.0
25	37.83329668243097	40.0	36.0	41.0	33.0	41.0
26	37.71534789654731	40.0	36.0	41.0	33.0	41.0
27	36.83208676107586	39.0	35.0	41.0	32.0	41.0
28	37.21891716454489	40.0	35.0	41.0	32.0	41.0
29	37.47962695563474	40.0	35.0	41.0	32.0	41.0
30	37.46530307646791	39.0	35.0	41.0	33.0	41.0
31	37.48777228750204	39.0	35.0	41.0	33.0	41.0
32	37.49671245821576	39.0	35.0	41.0	33.0	41.0
33	36.24938157121977	39.0	35.0	41.0	31.0	41.0
34	36.70636798389127	39.0	35.0	41.0	31.0	41.0
35	37.00594892718835	39.0	35.0	41.0	31.0	41.0
36	37.14504484215825	39.0	35.0	41.0	32.0	41.0
37	37.244435907286466	39.0	35.0	41.0	32.0	41.0
38	37.19733817301876	39.0	35.0	41.0	32.0	41.0
39	37.18759609822442	39.0	35.0	41.0	32.0	41.0
40	37.08386432984046	39.0	35.0	41.0	32.0	41.0
41	36.97471264367816	39.0	35.0	41.0	32.0	41.0
42	37.10542389197257	39.0	35.0	41.0	32.0	41.0
43	37.01416579455182	39.0	35.0	41.0	32.0	41.0
44	37.059565223151004	39.0	35.0	41.0	32.0	41.0
45	36.96078618393454	39.0	35.0	41.0	32.0	41.0
46	36.91099129651463	39.0	35.0	41.0	32.0	41.0
47	36.83272351530727	39.0	35.0	40.0	32.0	41.0
48	36.783764974984656	39.0	35.0	40.0	32.0	41.0
49	36.80889601300003	39.0	35.0	40.0	32.0	41.0
50	36.71070338822137	39.0	35.0	40.0	32.0	41.0
51	35.985571025474584	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	10.0
10	11.0
11	14.0
12	20.0
13	30.0
14	20.0
15	45.0
16	49.0
17	101.0
18	170.0
19	351.0
20	667.0
21	1184.0
22	1876.0
23	2783.0
24	3902.0
25	5255.0
26	6892.0
27	8284.0
28	10410.0
29	13784.0
30	19166.0
31	27414.0
32	40143.0
33	64921.0
34	85278.0
35	97782.0
36	127970.0
37	192582.0
38	223112.0
39	197916.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.056755909406032	4.441471158389303	52.215260022697066	16.286512909507596
2	20.34186901938965	6.2607689624262015	51.27840996904545	22.118952049138706
3	21.84870684135458	6.2960951333783735	49.51987317904628	22.33532484622076
4	20.18581565920843	4.668441806757014	49.92117848106297	25.224564052971594
5	16.42790590874367	23.252657190421306	39.52327332300043	20.796163577834594
6	16.921589147800283	30.925413205805857	38.34055311952168	13.812444526872177
7	81.78803414274422	2.302559822662622	10.747457619634286	5.161948414958867
8	82.8275950384393	3.9619183877135575	7.836051240610967	5.374435333236186
9	77.21099880332596	5.5417047526947245	9.785790930888762	7.461505513090554
10	40.36756880875736	26.87871200780708	17.633499807913946	15.120219375521613
11	32.25968268266942	22.562560440870612	25.707649440742557	19.470107435717406
12	28.801868754443372	20.315374391175524	29.868012593779948	21.014744260601162
13	27.25802676840604	21.581817619810916	31.935123487046337	19.22503212473671
14	21.033113869496294	24.25653865345468	33.35267441192965	21.357673065119382
15	19.398042046974975	22.45190121036293	36.24032394098763	21.909732801674462
16	24.458074458736824	22.82362084420717	29.435708576752727	23.282596120303275
17	24.66605729021774	22.847730955882028	28.698628019835642	23.787583734064587
18	25.175637306202837	21.032937238641534	31.10001280573697	22.691412649418663
19	25.574381460825485	23.02586317290836	28.27912973977859	23.120625626487563
20	24.51512622482458	27.968347750826855	28.9023717108023	18.614154313546262
21	24.77035780995403	24.945398987022045	30.72414234680585	19.560100856218067
22	22.462940638785486	22.290902186248402	31.5241034880178	23.722053686948303
23	21.407924543298844	24.44862470800712	30.836302939579003	23.307147809115037
24	22.669598738855697	25.360569811137456	28.72459275548549	23.245238694521355
25	23.96642247450996	26.445613151933443	25.78315913115283	23.80480524240377
26	22.06516795386402	23.998480974649055	27.475900927753568	26.46045014373336
27	21.00202683905838	25.602289135877697	30.27814943853467	23.11753458652925
28	18.872476938634026	24.072047725656954	29.329465117614074	27.72601021809495
29	21.75420933405752	25.09509363643188	28.079713504753578	25.070983524757022
30	23.26749418222122	24.396783552134803	29.422461262645665	22.913261002998308
31	23.015177006195326	24.96791942100406	27.710555018303374	24.30634855449724
32	25.153028556793444	24.56564264928619	25.439523803215565	24.8418049907048
33	23.215211449212006	26.664988673546436	25.871121296823734	24.24867858041782
34	21.27174215427822	24.239493775970256	29.43747488530034	25.051289184451186
35	21.53138951077669	25.595047270832506	27.36630148237445	25.507261736016357
36	24.010491872772796	25.376996480630222	27.157258865764966	23.45525278083202
37	20.024993265948662	26.762665536229196	28.4641505601406	24.748190637681546
38	21.197203933569135	25.61845085908832	26.399865760550384	26.784479446792165
39	20.87494093905794	25.17387099765522	26.9964364725052	26.954751590781633
40	23.10932125178287	24.576770393136123	28.066907767783416	24.247000587297592
41	20.262120188465122	24.122740780973324	27.426885865557427	28.188253165004127
42	20.16991888227995	22.466738202162844	30.808836841663684	26.55450607389352
43	21.088134380754305	22.150127395003995	30.894856067932224	25.866882156309472
44	21.907524915989953	22.084685663315096	28.9795593943328	27.028230026362156
45	20.7807966934704	22.417281562829803	26.750478007250695	30.0514437364491
46	22.00776292606674	23.44562639924755	28.401005029563592	26.145605645122117
47	18.034010271084206	24.66596897479036	32.243520959458806	25.05649979466663
48	18.921050423693263	25.81830867125024	29.48675489377862	25.77388601127788
49	22.153306750389692	22.48007383169729	30.61127523061366	24.755344187299357
50	20.59498103426197	23.739275195287487	28.613668578695666	27.05207519175487
51	19.48423790409828	23.71975748583641	26.50752226652713	30.28848234353818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	229.0
1	699.0
2	1169.0
3	2046.5
4	2924.0
5	2158.5
6	1393.0
7	1345.0
8	1297.0
9	1410.0
10	1523.0
11	1569.5
12	1616.0
13	1673.0
14	1730.0
15	1698.5
16	1667.0
17	1848.0
18	2029.0
19	2149.0
20	2269.0
21	2658.0
22	3047.0
23	3704.0
24	4361.0
25	5027.5
26	6961.5
27	8229.0
28	10489.0
29	12749.0
30	15569.0
31	18389.0
32	21260.0
33	24131.0
34	26291.0
35	28451.0
36	30448.0
37	32445.0
38	36320.5
39	40196.0
40	43656.5
41	47117.0
42	52181.5
43	57246.0
44	65276.5
45	73307.0
46	90560.5
47	107814.0
48	114956.0
49	122098.0
50	118801.5
51	115505.0
52	102759.5
53	90014.0
54	82907.5
55	75801.0
56	69258.0
57	62715.0
58	56844.0
59	50973.0
60	44545.0
61	38117.0
62	33922.5
63	29728.0
64	25791.5
65	21855.0
66	18700.5
67	15546.0
68	13303.5
69	11061.0
70	9304.0
71	7547.0
72	5984.5
73	4422.0
74	3634.5
75	2218.0
76	1589.0
77	1226.5
78	864.0
79	625.0
80	386.0
81	279.0
82	172.0
83	92.0
84	12.0
85	10.0
86	8.0
87	6.0
88	4.0
89	5.5
90	7.0
91	8.0
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1132305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.38475442424347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15232319096852	26.252445604420366
2	10.268957917147555	7.4726702401660905
3	4.011626725591427	4.378861597571281
4	2.2441885131957955	3.266169917373484
5	1.631038357870157	2.96724650538135
6	1.240925967907281	2.7090471960583833
7	1.0403480100740756	2.649696480360693
8	0.8815845915027013	2.566099109281823
9	0.7195039098563614	2.3561075760666053
>10	5.669154502659611	37.299260120015276
>50	0.0888144408764558	2.188375671889367
>100	0.04578009052854651	3.0685239842117644
>500	0.0035023019782951734	0.9638289129047206
>1k	0.002251479843189754	1.8616670842988243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	3445	0.3042466473255881	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	2944	0.26000061820799164	No Hit
CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	2761	0.2438388949973726	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	2560	0.2260874940939058	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	2381	0.21027903259280847	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	2054	0.18139988783940725	No Hit
TCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	1966	0.1736281302299292	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCCAACTT	1226	0.10827471396840957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2456935189723617	0.0
2	0.0	0.0	0.0	0.9429438181408719	0.0
3	0.0	0.0	0.0	1.303005815570893	0.0
4	0.0	0.0	0.0	1.7199429482339121	0.0
5	0.0	0.0	0.0	2.5901148542133083	0.0
6	0.0	0.0	0.0	3.1245998207196823	0.0
7	0.0	0.0	0.0	3.855674928574898	0.0
8	0.0	0.0	0.0	4.912192386327005	0.0
9	0.0	0.0	0.0	5.358980133444611	0.0
10	0.0	0.0	0.0	6.53233890162103	0.0
11	0.0	0.0	0.0	8.206534458471879	0.0
12	0.0	0.0	0.0	9.994656916643484	0.0
13	0.0	0.0	0.0	10.529053567722478	0.0
14	0.0	0.0	0.0	10.743836687111688	0.0
15	0.0	0.0	0.0	11.113790012408318	0.0
16	8.831542738043194E-5	0.0	0.0	11.757167900874764	0.0
17	8.831542738043194E-5	0.0	0.0	12.599962024366226	0.0
18	8.831542738043194E-5	0.0	0.0	13.488150277531231	0.0
19	1.766308547608639E-4	0.0	0.0	14.237065101717294	0.0
20	1.766308547608639E-4	0.0	0.0	14.920449878787077	0.0
21	1.766308547608639E-4	0.0	0.0	15.792211462459319	0.0
22	1.766308547608639E-4	0.0	0.0	16.752465104366756	0.0
23	1.766308547608639E-4	0.0	0.0	17.63049708338301	0.0
24	1.766308547608639E-4	0.0	0.0	18.31688458498373	0.0
25	1.766308547608639E-4	0.0	0.0	18.92290504766825	0.0
26	1.766308547608639E-4	0.0	0.0	19.44970657199253	0.0
27	1.766308547608639E-4	0.0	0.0	20.118960880681442	0.0
28	1.766308547608639E-4	0.0	0.0	20.7153549617815	0.0
29	1.766308547608639E-4	0.0	0.0	21.407218019879803	0.0
30	1.766308547608639E-4	0.0	0.0	22.196051417241822	0.0
31	2.6494628214129585E-4	0.0	0.0	22.789177827528803	0.0
32	2.6494628214129585E-4	0.0	0.0	23.380979506405076	0.0
33	2.6494628214129585E-4	0.0	0.0	23.987353230799123	0.0
34	2.6494628214129585E-4	0.0	0.0	24.68583994595096	0.0
35	2.6494628214129585E-4	0.0	0.0	25.40578730995624	0.0
36	2.6494628214129585E-4	0.0	0.0	26.002534652765817	0.0
37	2.6494628214129585E-4	0.0	0.0	26.633989958535906	0.0
38	2.6494628214129585E-4	0.0	0.0	27.37451481712083	0.0
39	2.6494628214129585E-4	0.0	0.0	28.721501715527175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	20	7.0332404E-4	45.000004	28
CCGAACG	20	7.0332404E-4	45.000004	15
TATCGTC	20	7.0332404E-4	45.000004	19
TACGTCG	20	7.0332404E-4	45.000004	39
ATCGAAT	35	1.2119017E-7	45.000004	43
TCGTTAG	140	0.0	43.39286	1
CGTTAGG	320	0.0	41.48438	2
TTATGGG	2925	0.0	40.53846	3
CGTTTAT	250	0.0	40.499996	1
TTACGGG	1120	0.0	40.379467	3
TACGGGA	660	0.0	40.227272	4
TTAGGGA	2360	0.0	40.13771	4
TATGGGC	910	0.0	39.80769	4
TATGGGA	1905	0.0	39.80315	4
CCGATGA	345	0.0	39.782608	18
TAGGGAG	1470	0.0	39.642857	5
TACGGGT	205	0.0	39.512196	4
TCGTCTA	40	3.4581535E-7	39.375004	39
TTTTACG	400	0.0	39.375	1
CGGGTAT	110	0.0	38.86364	6
>>END_MODULE