##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934183.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1016364
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08384004155992	33.0	31.0	34.0	30.0	34.0
2	32.31535158663628	34.0	31.0	34.0	30.0	34.0
3	32.40424985536678	34.0	31.0	34.0	30.0	34.0
4	35.77094131630007	37.0	35.0	37.0	35.0	37.0
5	29.387131972403587	37.0	35.0	37.0	0.0	37.0
6	23.601704704220143	35.0	10.0	37.0	0.0	37.0
7	30.352640392615243	35.0	19.0	37.0	17.0	37.0
8	34.313516614126435	35.0	32.0	37.0	32.0	37.0
9	36.97081950954579	38.0	37.0	39.0	34.0	39.0
10	37.13309404898245	39.0	37.0	39.0	34.0	39.0
11	37.247320841745676	39.0	37.0	39.0	34.0	39.0
12	37.09797080573495	39.0	37.0	39.0	34.0	39.0
13	36.944143043240416	39.0	37.0	39.0	33.0	39.0
14	37.8110509620569	40.0	37.0	41.0	33.0	41.0
15	37.79571393713276	40.0	37.0	41.0	33.0	41.0
16	38.12295299715456	40.0	37.0	41.0	33.0	41.0
17	38.183068270816364	40.0	37.0	41.0	33.0	41.0
18	38.25951430786608	40.0	37.0	41.0	34.0	41.0
19	38.265873250134796	40.0	37.0	41.0	34.0	41.0
20	38.1634985103762	40.0	37.0	41.0	33.0	41.0
21	38.05514166184556	40.0	37.0	41.0	33.0	41.0
22	38.18289707230874	40.0	37.0	41.0	34.0	41.0
23	38.164755933897695	40.0	36.0	41.0	34.0	41.0
24	38.13306256419944	40.0	36.0	41.0	34.0	41.0
25	37.902277136931254	40.0	36.0	41.0	34.0	41.0
26	37.771809115631804	40.0	36.0	41.0	33.0	41.0
27	36.90020209295095	40.0	35.0	41.0	33.0	41.0
28	37.24710438386248	40.0	35.0	41.0	33.0	41.0
29	37.52295142291541	40.0	35.0	41.0	33.0	41.0
30	37.55839148179196	40.0	35.0	41.0	33.0	41.0
31	37.55709273449276	39.0	35.0	41.0	33.0	41.0
32	37.54940946353865	40.0	35.0	41.0	33.0	41.0
33	36.320591835208646	39.0	35.0	41.0	31.0	41.0
34	36.752439086783866	39.0	35.0	41.0	31.0	41.0
35	37.03152807458745	39.0	35.0	41.0	31.0	41.0
36	37.15053366707203	39.0	35.0	41.0	32.0	41.0
37	37.20792747480233	39.0	35.0	41.0	33.0	41.0
38	37.13873080904086	39.0	35.0	41.0	33.0	41.0
39	37.14208689012992	39.0	35.0	41.0	33.0	41.0
40	37.08584030918057	39.0	35.0	41.0	33.0	41.0
41	36.94613052016797	39.0	35.0	41.0	32.0	41.0
42	36.99941851541377	39.0	35.0	41.0	33.0	41.0
43	36.89032275838184	39.0	35.0	41.0	32.0	41.0
44	36.905255400624185	39.0	35.0	41.0	33.0	41.0
45	36.849480107520534	39.0	35.0	40.0	33.0	41.0
46	36.82301911519888	39.0	35.0	40.0	33.0	41.0
47	36.75115411407724	38.0	35.0	40.0	32.0	41.0
48	36.67695038391757	38.0	35.0	40.0	32.0	41.0
49	36.68246907603969	38.0	35.0	40.0	32.0	41.0
50	36.576610348261056	38.0	35.0	40.0	32.0	41.0
51	35.860442715405114	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	16.0
11	16.0
12	23.0
13	24.0
14	32.0
15	46.0
16	61.0
17	119.0
18	181.0
19	298.0
20	472.0
21	924.0
22	1405.0
23	2228.0
24	3196.0
25	4475.0
26	5831.0
27	7311.0
28	8925.0
29	11811.0
30	16196.0
31	23558.0
32	34835.0
33	58774.0
34	78325.0
35	90993.0
36	116909.0
37	173192.0
38	199890.0
39	176150.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.059293717605108	4.294327622780815	54.43925601457745	17.20712264503662
2	19.192533383708987	4.995946334187358	53.73281619577238	22.078704086331275
3	22.069947381056394	5.038155621411226	51.45085815711694	21.44103884041544
4	19.602229122637166	4.701662002983183	50.19707506365829	25.499033810721354
5	15.747311002750983	23.339964815754985	39.8605224112621	21.052201770231925
6	16.93340181273638	30.372287881113458	38.68082694782578	14.01348335832438
7	83.81859255148746	2.13722642675262	9.047447567997292	4.996733453762628
8	85.57229496518963	2.7970294107229297	7.342448178014963	4.28822744607247
9	80.54387994852237	4.762565380119721	9.11563180120508	5.577922870152819
10	46.18404429908969	23.751431573727523	15.344010610371875	14.720513516810907
11	33.843091648267745	22.15436595550413	23.788524583712135	20.214017812515987
12	29.47841521344715	19.001558496759035	29.442404492878538	22.077621796915277
13	28.252378085016783	19.18968007524863	31.681070954894114	20.87687088484047
14	22.6076484409129	21.33841812578958	33.919639026962784	22.13429440633474
15	20.409912196811376	20.866835110255774	36.35075622513194	22.372496467800907
16	27.03932842957838	20.648212648224455	29.957279085052207	22.35517983714496
17	27.552825562495325	21.074241118339494	26.946546709643393	24.426386609521785
18	27.49290608482788	20.631781527090688	29.352869641191543	22.522442746889894
19	27.22194017104108	22.129866858723844	28.030410364790566	22.617782605444507
20	25.994919143141633	26.42891719895628	27.74537468859582	19.830788969306273
21	26.320393087515892	23.665340370182335	30.24674230885785	19.767524233443922
22	25.799418318633876	20.82757752143917	30.70189420325789	22.671109956669067
23	24.53796080931635	22.35606534666714	29.710025148470432	23.395948695546085
24	24.088121972049382	25.09474951887316	27.18317453195902	23.633953977118434
25	26.183631061312678	23.708927116662927	25.309731552868854	24.79771026915554
26	22.783274496145083	23.494830592189412	27.219677202262183	26.502217709403325
27	22.66176291171273	24.691252346600233	29.157565596577605	23.48941914510943
28	21.651199767012606	24.21553695329626	27.903388943331326	26.22987433635981
29	23.28378415607007	25.121905144219987	27.327119024286574	24.267191675423373
30	26.714149655044846	23.22799705617279	27.426984820398992	22.630868468383376
31	25.82391741541416	23.481547949356727	27.187798859463737	23.506735775765375
32	27.151984918788933	24.448327567682444	24.84562617330012	23.5540613402285
33	27.20452515043823	25.426422029902675	24.35633296732273	23.012719852336367
34	23.59853359623127	23.807218673624803	28.48024920205753	24.113998528086395
35	24.090581720722103	26.04362216686148	26.280249989177108	23.58554612323931
36	27.498317531907862	25.310715452337938	25.432718986504838	21.758248029249362
37	24.372468918615773	27.791617963642945	25.213211014951337	22.622702102789948
38	24.75796073060439	27.152083308735847	23.88435639200129	24.205599568658474
39	24.313926900205043	24.783443726853765	25.480339720808686	25.422289652132502
40	26.154802806868403	21.54887422222747	26.507924326324034	25.78839864458009
41	22.51201341251756	22.940698411199136	26.067137364172677	28.480150812110622
42	21.582720363964093	23.378828844783957	28.47670716396881	26.561743627283143
43	22.528247753757512	23.526413765147133	28.91837963564235	25.026958845453006
44	23.924401100393165	22.945814688438393	27.947172469705734	25.1826117414627
45	23.280832457662807	21.902192521576914	26.49719982211098	28.319775198649307
46	23.654812645863096	22.55117261138726	27.808836204351984	25.985178538397662
47	21.179124801744255	24.217898312022072	30.15927364605594	24.44370324017773
48	21.565403733308145	25.878917395736174	27.71369312569119	24.84198574526449
49	24.291985942044388	23.12567151138765	28.515472803050873	24.066869743517085
50	22.730931044389607	23.526413765147133	26.74455214863966	26.9981030418236
51	21.968704125687253	24.54819336379486	25.486833457304662	27.99626905321322
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	178.0
1	643.5
2	1109.0
3	2009.0
4	2909.0
5	2118.5
6	1328.0
7	1392.5
8	1457.0
9	1516.0
10	1575.0
11	1576.5
12	1578.0
13	1575.5
14	1573.0
15	1521.5
16	1470.0
17	1490.0
18	1510.0
19	1482.0
20	1454.0
21	1888.5
22	2323.0
23	2766.0
24	3209.0
25	3759.0
26	5292.5
27	6276.0
28	7841.0
29	9406.0
30	12027.0
31	14648.0
32	15883.0
33	17118.0
34	18836.5
35	20555.0
36	23157.0
37	25759.0
38	28555.0
39	31351.0
40	34883.0
41	38415.0
42	42577.5
43	46740.0
44	52374.0
45	58008.0
46	69236.0
47	80464.0
48	88686.5
49	96909.0
50	95337.5
51	93766.0
52	85160.5
53	76555.0
54	72508.5
55	68462.0
56	66537.5
57	64613.0
58	61134.0
59	57655.0
60	52986.5
61	48318.0
62	44315.5
63	40313.0
64	36638.5
65	32964.0
66	28222.5
67	23481.0
68	19526.5
69	15572.0
70	12614.0
71	9656.0
72	7768.0
73	5880.0
74	4891.0
75	2924.5
76	1947.0
77	1426.5
78	906.0
79	698.5
80	491.0
81	328.5
82	166.0
83	120.5
84	75.0
85	41.0
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1016364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.46237629140377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89212657710657	26.213577716436887
2	9.5396499983182	6.95676615853935
3	3.824502643952961	4.183513635938444
4	2.405508989306564	3.508422957618012
5	1.7582943837595646	3.2055795725851577
6	1.4215589158575157	3.1100049666238037
7	1.171711869366714	2.9906379360167286
8	0.9833602625425066	2.868452153827078
9	0.8422112871301861	2.7638122381387515
>10	6.051642549271128	38.17300185735965
>50	0.06951245775999283	1.7307416357057201
>100	0.03461600096861593	2.4149402988067044
>500	0.003349935574481634	0.8304951691443319
>1k	0.0019541290851142865	1.0500537032593367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	2008	0.19756701339283958	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	1738	0.17100172772746772	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	1533	0.15083178861116686	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	1283	0.12623430188397072	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	1278	0.12574235214942678	No Hit
TCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	1248	0.12279065374216323	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	1228	0.12082285480398754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.838994690878464E-5	0.0	0.0	0.18172623194052526	0.0
2	9.838994690878464E-5	0.0	0.0	0.6653128209972018	0.0
3	9.838994690878464E-5	0.0	0.0	0.9414934019701603	0.0
4	9.838994690878464E-5	0.0	0.0	1.229480776572173	0.0
5	9.838994690878464E-5	0.0	0.0	1.8326111511230228	0.0
6	9.838994690878464E-5	0.0	0.0	2.2869759259477904	0.0
7	9.838994690878464E-5	0.0	0.0	2.852226170938758	0.0
8	9.838994690878464E-5	0.0	0.0	3.6899181789201507	0.0
9	9.838994690878464E-5	0.0	0.0	4.067833964996792	0.0
10	9.838994690878464E-5	0.0	0.0	4.86469414501104	0.0
11	9.838994690878464E-5	0.0	0.0	6.191679358969818	0.0
12	9.838994690878464E-5	0.0	0.0	7.405811303824221	0.0
13	9.838994690878464E-5	0.0	0.0	7.865882695569698	0.0
14	9.838994690878464E-5	0.0	0.0	8.045542738625139	0.0
15	9.838994690878464E-5	0.0	0.0	8.35222420313982	0.0
16	9.838994690878464E-5	0.0	0.0	8.848306315453913	0.0
17	9.838994690878464E-5	0.0	0.0	9.528672798328158	0.0
18	1.9677989381756928E-4	0.0	0.0	10.255872895930985	0.0
19	1.9677989381756928E-4	0.0	0.0	10.808529227717628	0.0
20	1.9677989381756928E-4	0.0	0.0	11.342885029379238	0.0
21	1.9677989381756928E-4	0.0	0.0	12.056212144467926	0.0
22	1.9677989381756928E-4	0.0	0.0	12.795514205540535	0.0
23	1.9677989381756928E-4	0.0	0.0	13.470075681547161	0.0
24	1.9677989381756928E-4	0.0	0.0	14.0159431069971	0.0
25	1.9677989381756928E-4	0.0	0.0	14.53317905789658	0.0
26	1.9677989381756928E-4	0.0	0.0	15.011550979767092	0.0
27	1.9677989381756928E-4	0.0	0.0	15.607105328405964	0.0
28	1.9677989381756928E-4	0.0	0.0	16.09649692433026	0.0
29	1.9677989381756928E-4	0.0	0.0	16.657909961391784	0.0
30	1.9677989381756928E-4	0.0	0.0	17.244609214808868	0.0
31	1.9677989381756928E-4	0.0	0.0	17.71412604145759	0.0
32	1.9677989381756928E-4	0.0	0.0	18.219358418834197	0.0
33	1.9677989381756928E-4	0.0	0.0	18.698615850226886	0.0
34	1.9677989381756928E-4	0.0	0.0	19.2957444380163	0.0
35	1.9677989381756928E-4	0.0	0.0	19.86256892215781	0.0
36	1.9677989381756928E-4	0.0	0.0	20.34428610222322	0.0
37	1.9677989381756928E-4	0.0	0.0	20.890251917620066	0.0
38	1.9677989381756928E-4	0.0	0.0	21.554088889413634	0.0
39	1.9677989381756928E-4	0.0	0.0	22.794195780251957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	50	2.1827873E-11	45.0	14
CGTAATT	20	7.032902E-4	45.0	12
AACTCGT	25	3.890487E-5	45.0	30
CGTTAGG	315	0.0	42.142857	2
TACGGGC	230	0.0	42.06522	4
GATAACG	55	6.184564E-11	40.909092	17
TTATGGG	2645	0.0	40.74669	3
CGTTTAT	205	0.0	40.609756	1
TTAGGGA	1840	0.0	40.475548	4
CTCAACT	695	0.0	40.467625	2
TTACGGG	965	0.0	40.33679	3
TCGTCAG	45	1.9279469E-8	40.0	1
CGTATGG	215	0.0	39.767445	2
ACTTGAC	685	0.0	39.416058	6
GCGCATA	40	3.4576988E-7	39.375	27
ATGGGAC	565	0.0	39.026546	5
GCGTTAG	150	0.0	39.000004	1
TAGGGAG	1200	0.0	39.000004	5
TACGGGT	110	0.0	38.863636	4
TGCATTA	565	0.0	38.628315	15
>>END_MODULE