FastQCFastQC Report
Sat 14 Jan 2017
SRR2934182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971629
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC108491.1165784471233362No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG89360.9196925987182349No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC68050.700370203030169No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTAT41920.43144039545958385No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC29520.3038196677950123TruSeq Adapter, Index 13 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC27090.2788101219704229No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC25220.2595640928790722No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT24370.2508158978375491TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC23630.2431998221543408TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT22690.23352534763783295No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTA18520.19060773196353753No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT17660.18175661698034948No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC16280.16755366503058267TruSeq Adapter, Index 13 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC15210.1565412312724301No Hit
GCTGTATCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC11850.12196013087299783TruSeq Adapter, Index 16 (95% over 22bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC11060.11382945548146463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT302.165154E-645.00000436
TACGCGC302.165154E-645.00000434
CGATAGT351.2116652E-745.00000424
AACGTAT207.0327485E-445.032
GAATCGT207.0327485E-445.012
CCCCGTA207.0327485E-445.040
TTACGTC207.0327485E-445.024
TAGGGAC7500.042.35
TAGGGAT9350.042.1122975
AATACGG5450.042.110095
TCGTTAG1500.042.01
CGATCGA750.042.041
CCGATGA18650.041.8632718
ACCCGTC700.041.78571739
CTAGGGA11150.041.7712974
TAATACG5450.041.6972474
CGATGAA19000.041.68420819
TACGGCT15150.041.4356467
CGTTAGG2800.040.9821432
TTAGGGA19150.040.652744
Produced by FastQC (version 0.11.5)