##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934182.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	971629
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.841367435512936	31.0	31.0	34.0	30.0	34.0
2	31.962372469327285	33.0	31.0	34.0	30.0	34.0
3	32.05628279929891	33.0	31.0	34.0	30.0	34.0
4	35.63816950708552	37.0	35.0	37.0	33.0	37.0
5	29.38544444433009	37.0	35.0	37.0	0.0	37.0
6	23.548964676846822	35.0	10.0	37.0	0.0	37.0
7	30.285003844059823	35.0	19.0	37.0	17.0	37.0
8	33.852839921410336	35.0	32.0	37.0	30.0	37.0
9	36.694706518640345	37.0	35.0	39.0	33.0	39.0
10	36.888311279305164	39.0	37.0	39.0	33.0	39.0
11	37.081566112168325	39.0	37.0	39.0	34.0	39.0
12	37.01874892577311	39.0	37.0	39.0	33.0	39.0
13	36.89084928506662	39.0	36.0	39.0	33.0	39.0
14	37.844044383195644	40.0	37.0	41.0	33.0	41.0
15	37.71389285416553	40.0	37.0	41.0	33.0	41.0
16	38.07211188632698	40.0	37.0	41.0	33.0	41.0
17	38.01043505288541	40.0	37.0	41.0	33.0	41.0
18	38.12371491587839	40.0	37.0	41.0	34.0	41.0
19	38.12875799301997	40.0	37.0	41.0	34.0	41.0
20	37.88913360963907	40.0	37.0	41.0	33.0	41.0
21	37.898471535946335	40.0	36.0	41.0	33.0	41.0
22	38.02068484987583	40.0	37.0	41.0	34.0	41.0
23	37.84652578298919	40.0	36.0	41.0	33.0	41.0
24	37.870681093297954	40.0	36.0	41.0	33.0	41.0
25	37.73874596167879	40.0	36.0	41.0	33.0	41.0
26	37.60225044744445	40.0	36.0	41.0	33.0	41.0
27	36.65046432331682	39.0	35.0	41.0	31.0	41.0
28	37.0162201828064	39.0	35.0	41.0	31.0	41.0
29	37.350328160234	39.0	35.0	41.0	32.0	41.0
30	37.36557986638933	39.0	35.0	41.0	32.0	41.0
31	37.399535213543444	39.0	35.0	41.0	33.0	41.0
32	37.246345055571624	39.0	35.0	41.0	32.0	41.0
33	36.02366644058586	39.0	35.0	41.0	30.0	41.0
34	36.47149992435384	39.0	35.0	41.0	30.0	41.0
35	36.76358466040021	39.0	35.0	41.0	30.0	41.0
36	36.819670882610545	39.0	35.0	41.0	31.0	41.0
37	36.84863770019215	39.0	35.0	41.0	31.0	41.0
38	36.838203676506154	39.0	35.0	41.0	31.0	41.0
39	36.82851890999548	39.0	35.0	41.0	31.0	41.0
40	36.907829017042516	39.0	35.0	41.0	31.0	41.0
41	36.77179561334625	39.0	35.0	41.0	31.0	41.0
42	36.69942333956685	39.0	35.0	41.0	31.0	41.0
43	36.619759187920494	39.0	35.0	41.0	31.0	41.0
44	36.6986164472242	39.0	35.0	41.0	31.0	41.0
45	36.556883337158524	39.0	35.0	41.0	31.0	41.0
46	36.506320828217355	39.0	35.0	40.0	31.0	41.0
47	36.36815904012746	39.0	35.0	40.0	31.0	41.0
48	36.365615888368914	39.0	35.0	40.0	31.0	41.0
49	36.41551559288576	39.0	35.0	40.0	31.0	41.0
50	36.32906901708368	39.0	35.0	40.0	31.0	41.0
51	35.6557791091044	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	2.0
10	12.0
11	10.0
12	17.0
13	5.0
14	23.0
15	28.0
16	46.0
17	97.0
18	169.0
19	380.0
20	770.0
21	1802.0
22	3409.0
23	5140.0
24	6780.0
25	7702.0
26	7797.0
27	8149.0
28	9291.0
29	11712.0
30	16118.0
31	23086.0
32	33470.0
33	52130.0
34	69940.0
35	85224.0
36	110868.0
37	164241.0
38	189105.0
39	163978.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.236099375378874	4.066984414833233	48.751323807749664	15.945592402038228
2	18.80223830289133	12.435610711495848	47.25991093308248	21.50224005253034
3	19.72717981863448	12.786258952748424	46.77207040959049	20.714490819026604
4	19.184894646001716	4.512216082475924	52.54124773962078	23.761641531901578
5	19.826394642399517	22.126861178495083	36.92211739254386	21.12462678656154
6	16.163473918542984	34.1990615759719	36.16112734387302	13.476337161612099
7	77.57189215225154	2.1241646760234616	15.474424909095962	4.829518262629049
8	77.45044662108685	8.403824916712036	8.443037414486392	5.702691047714714
9	72.8683478982204	4.864613962736806	9.842028181538428	12.425009957504356
10	41.32019525971333	26.500238259664954	16.742398590408477	15.43716789021324
11	33.65770268281412	21.451500521289503	24.67876113207819	20.212035663818188
12	25.985432711456742	19.919228429781327	29.728630989811954	24.36670786894998
13	26.965127636165654	21.113820192686713	32.9239864186845	18.997065752463133
14	20.497535581996832	27.134225100321213	31.14480938712204	21.223429930559913
15	17.834379171473884	21.84331673920807	36.76650244074642	23.555801648571624
16	21.825202829475035	22.99303540754753	29.029495826081764	26.15226593689567
17	22.178526989210905	24.80072126295119	29.521350227298694	23.499401520539216
18	22.512193440088758	21.46076331603935	30.39349381296771	25.633549430904186
19	22.521353314896942	23.827510294567166	27.60467215367182	26.046464236864068
20	25.295045742768075	26.069724143680357	30.001368835224145	18.633861278327426
21	23.73519110689368	28.028805233273197	29.03495058299001	19.201053076843117
22	21.607424232911946	21.664544800536007	32.42132542359275	24.306705542959296
23	22.86562051976629	25.122243160712575	29.72646967103699	22.285666648484142
24	22.146724727236425	25.954968408723904	27.57009105327239	24.32821581076728
25	21.626361502178302	29.33897609066835	24.759759126168525	24.27490328098482
26	21.019751366004925	23.240043267543477	28.789177762294045	26.951027604157552
27	23.80425038775088	25.30080925950131	27.281503536843793	23.613436815904013
28	17.380605148673002	25.9408683767158	31.029539052457267	25.64898742215393
29	21.23979420128465	22.274036695076003	30.181684573021183	26.304484530618165
30	23.15060583823661	24.56565211618838	29.05604917103133	23.227692874543678
31	22.64907696250318	22.319012709583596	28.312864272268527	26.719046055644696
32	25.148899425603805	24.071533476254825	27.78241489292724	22.99715220521413
33	23.59130902844604	24.661676421761804	24.496695755272846	27.250318794519305
34	20.063625107937288	22.541731463346608	29.439631793616698	27.955011635099403
35	22.136844412836588	23.318674102975518	28.90074297905888	25.643738505129015
36	22.23266287852668	26.48469734847354	25.78875270293497	25.493887070064808
37	21.75768734774281	24.17610013698644	30.127754523588735	23.93845799168201
38	20.50865093569665	24.10992261449586	29.183361138870907	26.19806531093658
39	21.080988731295587	23.749291138901782	28.4933858499489	26.67633427985373
40	21.849697775591302	21.077386533337314	28.852679366301338	28.220236324770053
41	19.54233560340418	24.387703537049635	26.085470894755098	29.984489964791088
42	21.251321234751124	22.418433373231963	31.48454811455813	24.845697277458783
43	21.650650608411233	24.062991121096633	27.782929492635567	26.50342877785657
44	22.9807879344894	20.77706614355891	29.554902128281473	26.687243793670216
45	21.195744466252037	19.935489780564392	27.93628020571638	30.93248554746719
46	23.4295188801487	22.291327245275717	28.48463765490738	25.794516219668207
47	17.31463346606575	23.92188788107395	34.128767255814715	24.63471139704558
48	19.41409735608962	24.88038129779988	29.40433025362561	26.30119109248489
49	21.67607183400248	20.53551304047121	33.681785949163725	24.10662917636258
50	20.98259726706387	22.660603995969655	28.930898521966718	27.42590021499976
51	19.311897854016298	21.35959301338268	27.674966473829006	31.65354265877202
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	531.5
2	951.0
3	1969.5
4	2988.0
5	2129.5
6	1271.0
7	1265.5
8	1260.0
9	1356.5
10	1453.0
11	1447.0
12	1441.0
13	1488.5
14	1536.0
15	1535.0
16	1534.0
17	1613.5
18	1693.0
19	1783.0
20	1873.0
21	2242.0
22	2611.0
23	3158.0
24	3705.0
25	4351.0
26	5874.0
27	6751.0
28	8545.0
29	10339.0
30	11605.0
31	12871.0
32	15578.0
33	18285.0
34	20526.5
35	22768.0
36	24879.0
37	26990.0
38	29381.5
39	31773.0
40	33886.5
41	36000.0
42	40770.0
43	45540.0
44	54936.0
45	64332.0
46	91231.5
47	118131.0
48	111618.5
49	105106.0
50	100852.5
51	96599.0
52	86892.0
53	77185.0
54	70910.5
55	64636.0
56	59060.0
57	53484.0
58	47961.5
59	42439.0
60	37377.5
61	32316.0
62	28313.5
63	24311.0
64	21081.0
65	17851.0
66	15483.0
67	13115.0
68	11001.5
69	8888.0
70	7359.0
71	5830.0
72	4935.5
73	4041.0
74	3217.0
75	1798.5
76	1204.0
77	900.0
78	596.0
79	422.5
80	249.0
81	172.5
82	96.0
83	76.5
84	57.0
85	39.0
86	21.0
87	10.5
88	0.0
89	2.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	971629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.17645934212943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.88071925487287	27.094470962039303
2	10.16815046817302	7.56031664929377
3	3.8803603934390045	4.3277418119848345
4	2.23329345029284	3.3210377261542288
5	1.5988602691106584	2.971998189416925
6	1.279644346017882	2.854358760127175
7	1.058867718835498	2.7555466888586855
8	0.892349672697654	2.6539521060805464
9	0.7452701375393467	2.493585447042125
>10	5.1442389586146735	32.47158231738462
>50	0.07285548641588309	1.8475878314100789
>100	0.03729483996750212	2.809415873439702
>500	0.0034692874385297803	0.917125584259989
>1k	0.003758394725073929	3.063390765566414
>5k	5.782145730882967E-4	1.6918403635106634
>10k+	2.8910728654414836E-4	1.166048923430988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC	10849	1.1165784471233362	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	8936	0.9196925987182349	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	6805	0.700370203030169	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTAT	4192	0.43144039545958385	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	2952	0.3038196677950123	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	2709	0.2788101219704229	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC	2522	0.2595640928790722	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	2437	0.2508158978375491	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	2363	0.2431998221543408	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT	2269	0.23352534763783295	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTA	1852	0.19060773196353753	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT	1766	0.18175661698034948	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	1628	0.16755366503058267	TruSeq Adapter, Index 13 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	1521	0.1565412312724301	No Hit
GCTGTATCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	1185	0.12196013087299783	TruSeq Adapter, Index 16 (95% over 22bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC	1106	0.11382945548146463	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0291994166497707E-4	0.0	0.0	0.2674889283872754	0.0
2	1.0291994166497707E-4	0.0	0.0	1.0446374078995173	0.0
3	1.0291994166497707E-4	0.0	0.0	1.3994024468186932	0.0
4	1.0291994166497707E-4	0.0	0.0	1.9637124869677625	0.0
5	1.0291994166497707E-4	0.0	0.0	3.3271958741453784	0.0
6	1.0291994166497707E-4	0.0	0.0	3.836443745503685	0.0
7	1.0291994166497707E-4	0.0	0.0	4.720834804230833	0.0
8	1.0291994166497707E-4	0.0	0.0	5.928188639902679	0.0
9	1.0291994166497707E-4	0.0	0.0	6.318975658404597	0.0
10	1.0291994166497707E-4	0.0	0.0	8.258193199256095	0.0
11	1.0291994166497707E-4	0.0	0.0	9.788406891930974	0.0
12	1.0291994166497707E-4	0.0	0.0	12.551601485752277	0.0
13	1.0291994166497707E-4	0.0	0.0	13.1045903323182	0.0
14	1.0291994166497707E-4	0.0	0.0	13.35396535097244	0.0
15	1.0291994166497707E-4	0.0	0.0	13.871446817663944	0.0
16	1.0291994166497707E-4	0.0	0.0	14.538059279828	0.0
17	1.0291994166497707E-4	0.0	0.0	15.341143584639816	0.0
18	1.0291994166497707E-4	0.0	0.0	16.13712641347675	0.0
19	1.0291994166497707E-4	0.0	0.0	17.266775693191537	0.0
20	1.0291994166497707E-4	0.0	0.0	17.949134906430334	0.0
21	1.0291994166497707E-4	0.0	0.0	18.80697262020792	0.0
22	1.0291994166497707E-4	0.0	0.0	19.698259315026622	0.0
23	1.0291994166497707E-4	0.0	0.0	20.496197622755187	0.0
24	2.0583988332995414E-4	0.0	0.0	21.150974291627772	0.0
25	2.0583988332995414E-4	0.0	0.0	21.675660154235825	0.0
26	2.0583988332995414E-4	0.0	0.0	22.17389559183598	0.0
27	2.0583988332995414E-4	0.0	0.0	22.718136243360377	0.0
28	2.0583988332995414E-4	0.0	0.0	23.271948449459618	0.0
29	2.0583988332995414E-4	0.0	0.0	23.87063375012479	0.0
30	2.0583988332995414E-4	0.0	0.0	24.579443388371487	0.0
31	2.0583988332995414E-4	0.0	0.0	25.115965044271015	0.0
32	2.0583988332995414E-4	0.0	0.0	25.61944939889608	0.0
33	2.0583988332995414E-4	0.0	0.0	26.162763770945496	0.0
34	2.0583988332995414E-4	0.0	0.0	26.745290640769266	0.0
35	2.0583988332995414E-4	0.0	0.0	27.380203760900507	0.0
36	2.0583988332995414E-4	0.0	0.0	27.927120330908195	0.0
37	2.0583988332995414E-4	0.0	0.0	28.488342772807318	0.0
38	2.0583988332995414E-4	0.0	0.0	29.13087196862177	0.0
39	2.0583988332995414E-4	0.0	0.0	30.14772099227174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAT	30	2.165154E-6	45.000004	36
TACGCGC	30	2.165154E-6	45.000004	34
CGATAGT	35	1.2116652E-7	45.000004	24
AACGTAT	20	7.0327485E-4	45.0	32
GAATCGT	20	7.0327485E-4	45.0	12
CCCCGTA	20	7.0327485E-4	45.0	40
TTACGTC	20	7.0327485E-4	45.0	24
TAGGGAC	750	0.0	42.3	5
TAGGGAT	935	0.0	42.112297	5
AATACGG	545	0.0	42.11009	5
TCGTTAG	150	0.0	42.0	1
CGATCGA	75	0.0	42.0	41
CCGATGA	1865	0.0	41.86327	18
ACCCGTC	70	0.0	41.785717	39
CTAGGGA	1115	0.0	41.771297	4
TAATACG	545	0.0	41.697247	4
CGATGAA	1900	0.0	41.684208	19
TACGGCT	1515	0.0	41.435646	7
CGTTAGG	280	0.0	40.982143	2
TTAGGGA	1915	0.0	40.65274	4
>>END_MODULE