FastQCFastQC Report
Sat 14 Jan 2017
SRR2934181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345448
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC142311.0577146050980788No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG122070.9072814408286312No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC88110.6548748074990636No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACCATTGT50430.37481939101325357No Hit
GAATCAGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC32230.2395484626681968No Hit
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC30920.22981192881478882TruSeq Adapter, Index 27 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT29970.2227510836539205No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC29070.21606186192257149No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTA22040.16381160773214573No Hit
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC20910.15541291822500758TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT20570.15288587890427574TruSeq Adapter, Index 20 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC19830.14738585214738883No Hit
GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT18480.13735201955036538No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.12806143381238072No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC15740.11698705561270298No Hit
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC14810.11007485982364239TruSeq Adapter, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG253.8911618E-545.040
CGATCGA750.045.041
TAGGGCG5900.043.093225
AATACGG7100.042.7816935
CGTTAGG4750.042.6315772
TACGGCT21350.042.4707267
CGATGAA26600.041.95488719
CCGATGA26550.041.77966318
ACGGCTG21950.041.514818
TATGGGA26900.041.4033434
TAATACG7400.041.3513534
TTATGGG34900.041.1962743
TCGTTAG2350.041.170211
TTAGGGA33400.041.1601834
CCGATCG556.184564E-1140.90909240
TAGGGAT18600.040.766135
GATGAAT29050.040.58519720
TAGGGAG15000.040.55
TAACGCG451.9286745E-840.00000426
AACGCGA451.9286745E-840.00000427
Produced by FastQC (version 0.11.5)