##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934181.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1345448 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90316088024212 33.0 31.0 34.0 30.0 34.0 2 32.035736052229446 33.0 31.0 34.0 30.0 34.0 3 32.1344355188755 34.0 31.0 34.0 30.0 34.0 4 35.689824504551645 37.0 35.0 37.0 35.0 37.0 5 29.40108796475226 37.0 35.0 37.0 0.0 37.0 6 23.573582182291698 35.0 10.0 37.0 0.0 37.0 7 30.33064897342744 35.0 19.0 37.0 17.0 37.0 8 33.9558964746315 35.0 32.0 37.0 32.0 37.0 9 36.780073254410425 37.0 37.0 39.0 34.0 39.0 10 36.94210181292774 39.0 37.0 39.0 34.0 39.0 11 37.08747495258085 39.0 37.0 39.0 34.0 39.0 12 37.034861993923215 39.0 37.0 39.0 34.0 39.0 13 36.91300741463066 39.0 37.0 39.0 33.0 39.0 14 37.83644258269364 40.0 37.0 41.0 33.0 41.0 15 37.726775765395615 40.0 37.0 41.0 32.0 41.0 16 38.08519913069848 40.0 37.0 41.0 33.0 41.0 17 38.030065078694975 40.0 37.0 41.0 33.0 41.0 18 38.13764039933167 40.0 37.0 41.0 33.0 41.0 19 38.13438869432338 40.0 37.0 41.0 34.0 41.0 20 37.898132071993864 40.0 37.0 41.0 33.0 41.0 21 37.92637099315618 40.0 37.0 41.0 33.0 41.0 22 38.06078272813219 40.0 37.0 41.0 34.0 41.0 23 37.88678492219692 40.0 36.0 41.0 33.0 41.0 24 37.91512641142579 40.0 36.0 41.0 33.0 41.0 25 37.769474554200535 40.0 36.0 41.0 33.0 41.0 26 37.65063384092139 40.0 36.0 41.0 33.0 41.0 27 36.68910504159209 39.0 35.0 41.0 31.0 41.0 28 37.071480280174335 40.0 35.0 41.0 31.0 41.0 29 37.366252727715974 40.0 35.0 41.0 32.0 41.0 30 37.357674915715805 39.0 35.0 41.0 32.0 41.0 31 37.46434644817191 40.0 36.0 41.0 33.0 41.0 32 37.28464719558095 40.0 35.0 41.0 32.0 41.0 33 36.03023082274454 39.0 35.0 41.0 30.0 41.0 34 36.44095869925854 39.0 35.0 41.0 30.0 41.0 35 36.67510821674268 39.0 35.0 41.0 30.0 41.0 36 36.77698877994541 39.0 35.0 41.0 31.0 41.0 37 36.833314256663954 39.0 35.0 41.0 31.0 41.0 38 36.86716840784631 39.0 35.0 41.0 31.0 41.0 39 36.828738085752846 39.0 35.0 41.0 31.0 41.0 40 36.75203798288749 39.0 35.0 41.0 31.0 41.0 41 36.67943837294344 39.0 35.0 41.0 31.0 41.0 42 36.6898088963676 39.0 35.0 41.0 31.0 41.0 43 36.54345467086056 39.0 35.0 41.0 31.0 41.0 44 36.65635461199541 39.0 35.0 41.0 31.0 41.0 45 36.60277245943359 39.0 35.0 41.0 31.0 41.0 46 36.54621063021388 39.0 35.0 41.0 31.0 41.0 47 36.449316510188424 39.0 35.0 41.0 31.0 41.0 48 36.4337848805751 39.0 35.0 41.0 31.0 41.0 49 36.43673780034606 39.0 35.0 40.0 31.0 41.0 50 36.27641276362966 39.0 35.0 40.0 31.0 41.0 51 35.634377545620495 38.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 6.0 10 8.0 11 10.0 12 11.0 13 12.0 14 23.0 15 25.0 16 54.0 17 112.0 18 275.0 19 643.0 20 1757.0 21 3597.0 22 5540.0 23 7376.0 24 9115.0 25 10310.0 26 11038.0 27 11759.0 28 13171.0 29 16722.0 30 22516.0 31 32164.0 32 45738.0 33 71780.0 34 94739.0 35 110663.0 36 145551.0 37 220054.0 38 264028.0 39 246461.0 40 190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.63094820461289 3.7818629928469925 50.79668630820366 15.790502494336458 2 18.479420980966935 11.755415296614956 49.52439633490109 20.24076738751702 3 19.109174044630485 11.873814521259833 48.493438616728405 20.523572817381275 4 17.52182172778138 4.107256467734167 55.26486345068706 23.106058353797394 5 19.484365059073262 22.061424893418398 38.94546649145861 19.508743556049733 6 15.586852854959835 33.78376570480613 37.64857504712185 12.980806393112182 7 79.18373656952926 1.6608594312080438 14.952565985456145 4.202838013806554 8 80.54402697094201 7.701746927417484 7.420502315957213 4.333723785683281 9 75.8338486511556 4.58382635374983 8.404263858580933 11.178061136513637 10 41.82748051206735 29.736860882025912 15.24384442951344 13.19181417639329 11 31.902607904578993 21.743240913063904 27.06392220286477 19.290228979492333 12 25.875247501204058 20.05198268532117 30.25438366997461 23.81838614350016 13 27.456207895065436 20.59098530749609 33.68684631438747 18.265960483051 14 20.864425826936454 25.891152983987492 31.91747284175977 21.326948347316286 15 17.93588455295188 22.3528519868475 36.85619957070062 22.855063889500002 16 22.83224621092751 23.312978279353793 28.548780777852436 25.30599473186626 17 23.434127517377114 25.10673024895797 29.673387600264 21.78575463340092 18 23.1866263133172 22.31464909829291 30.251559331910265 24.247165256479626 19 22.987584804466614 24.486490745090112 28.08083255540162 24.445091895041653 20 24.827195105273486 27.280207038845056 30.026578507679226 17.866019348202233 21 23.544648325316178 28.442273502952176 29.646110440537278 18.366967731194368 22 21.425131257395304 21.533868272872677 33.95493545644276 23.086065013289254 23 22.32750726895428 25.459623857629577 30.99198185288469 21.22088702053145 24 22.03280988934541 26.059721371617485 28.42971263103442 23.477756108002687 25 22.440480791528174 29.909219828637003 25.03404070614398 22.616258673690844 26 21.105163484579116 24.28462489817518 29.24356794168188 25.36664367556383 27 22.46812957468442 26.67520409558749 28.146981525856074 22.70968480387202 28 18.172385703498016 26.558142715288884 29.745705519648475 25.523766061564622 29 22.724029468251466 24.02218443224859 28.518530630689554 24.735255468810387 30 22.309892318395065 28.398124639525275 28.37515831158098 20.91682473049869 31 22.677502214875638 24.075326582669863 26.74759633965787 26.49957486279663 32 26.313094225863804 25.84737574399011 25.311346109251343 22.52818392089475 33 24.44107836200284 26.85246847146824 24.953844370053694 23.752608796475226 34 22.707157764551287 23.442972154999673 29.65406318192899 24.195806898520047 35 22.30506121381131 26.241073605222944 28.385415118235713 23.068450062730033 36 24.982459374126684 27.61786408690637 25.048608344581137 22.35106819438581 37 20.327281321909133 27.17243624428443 28.11531920966102 24.384963224145416 38 21.29350223865954 25.630496310522595 25.928835599740758 27.147165851077116 39 22.17335787038964 24.825857260927215 27.585755822595893 25.415029046087252 40 21.91091740446305 24.74580957420874 29.693380940772148 23.649892080556068 41 21.564787342208692 23.64647314500449 28.735781687586588 26.05295782520023 42 21.54330750798247 22.488048590506658 31.8930943447833 24.075549556727573 43 23.931062367330437 23.224457578442273 28.00777138915811 24.83670866506918 44 22.140655008591935 23.259464505503 29.030479067195465 25.569401418709603 45 21.070305206890197 21.69559878939952 27.54718131061178 29.686914693098508 46 23.691662554034046 24.167712167248382 27.36463988203186 24.775985396685712 47 17.808566365998537 25.839274353226582 33.34435816174241 23.00780111903247 48 20.349876026423914 25.91374768850227 28.990715360236884 24.745660924836933 49 21.896126791968175 21.85116035699633 32.34922494217539 23.903487908860097 50 21.795862790683845 23.09884885926472 28.859383640244733 26.245904709806695 51 20.4320791290336 22.445088922054214 28.16184646303685 28.960985485875334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 721.0 2 1246.0 3 3460.0 4 5674.0 5 3937.0 6 2200.0 7 2132.0 8 2064.0 9 2145.0 10 2226.0 11 2225.0 12 2224.0 13 2194.5 14 2165.0 15 2168.0 16 2171.0 17 2252.0 18 2333.0 19 2379.5 20 2426.0 21 2971.5 22 3517.0 23 4174.5 24 4832.0 25 6204.0 26 9578.0 27 11580.0 28 13994.5 29 16409.0 30 19073.5 31 21738.0 32 26204.5 33 30671.0 34 34206.5 35 37742.0 36 41098.0 37 44454.0 38 47799.5 39 51145.0 40 54866.0 41 58587.0 42 64440.5 43 70294.0 44 81696.0 45 93098.0 46 126507.5 47 159917.0 48 149156.5 49 138396.0 50 131312.0 51 124228.0 52 111559.5 53 98891.0 54 91416.0 55 83941.0 56 77670.5 57 71400.0 58 64484.0 59 57568.0 60 50520.0 61 43472.0 62 37629.0 63 31786.0 64 27432.5 65 23079.0 66 18365.0 67 13651.0 68 11316.0 69 8981.0 70 7308.5 71 5636.0 72 4616.0 73 3596.0 74 2867.0 75 1631.5 76 1125.0 77 854.0 78 583.0 79 423.0 80 263.0 81 227.0 82 191.0 83 109.5 84 28.0 85 18.5 86 9.0 87 5.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1345448.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.26654197295092 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64337701015012 25.26614162412406 2 9.425627044231325 6.648185435535306 3 3.7664065218163567 3.9848440106649794 4 2.477233583425771 3.4945384858675426 5 1.839841802144843 3.2442429069465386 6 1.5093157890263251 3.193700917448066 7 1.2407471807418593 3.062980376921732 8 1.0721610435258624 3.0249129954614147 9 0.921692822254328 2.925442674197986 >10 5.988882951187684 34.387132203256826 >50 0.07098819362153629 1.7084381300272389 >100 0.03525556578055851 2.3512885740003724 >500 0.004349712156117912 1.0501393565032113 >1k 0.0032050510624026724 2.4049781772520693 >5k 4.5786443748609606E-4 1.1185231525329047 >10k+ 4.5786443748609606E-4 2.134510979259776 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC 14231 1.0577146050980788 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG 12207 0.9072814408286312 No Hit GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC 8811 0.6548748074990636 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACCATTGT 5043 0.37481939101325357 No Hit GAATCAGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC 3223 0.2395484626681968 No Hit GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC 3092 0.22981192881478882 TruSeq Adapter, Index 27 (95% over 21bp) GAATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT 2997 0.2227510836539205 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC 2907 0.21606186192257149 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTA 2204 0.16381160773214573 No Hit CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC 2091 0.15541291822500758 TruSeq Adapter, Index 27 (95% over 21bp) CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT 2057 0.15288587890427574 TruSeq Adapter, Index 20 (95% over 22bp) GAATATGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC 1983 0.14738585214738883 No Hit GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT 1848 0.13735201955036538 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1723 0.12806143381238072 No Hit GAATAATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC 1574 0.11698705561270298 No Hit TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC 1481 0.11007485982364239 TruSeq Adapter, Index 27 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4864937180775474E-4 0.0 0.0 0.1648521533348 0.0 2 1.4864937180775474E-4 0.0 0.0 0.7021453077339295 0.0 3 1.4864937180775474E-4 0.0 0.0 0.9630249552565391 0.0 4 1.4864937180775474E-4 0.0 0.0 1.3751553385935391 0.0 5 1.4864937180775474E-4 0.0 0.0 2.564350313055577 0.0 6 1.4864937180775474E-4 0.0 0.0 3.0398053287826805 0.0 7 1.4864937180775474E-4 0.0 0.0 3.8385727281916506 0.0 8 1.4864937180775474E-4 0.0 0.0 4.8890778387570535 0.0 9 1.4864937180775474E-4 0.0 0.0 5.2656066975460964 0.0 10 1.4864937180775474E-4 0.0 0.0 7.098453451935712 0.0 11 1.4864937180775474E-4 0.0 0.0 8.38850702516931 0.0 12 1.4864937180775474E-4 0.0 0.0 10.820262098572371 0.0 13 1.4864937180775474E-4 0.0 0.0 11.276392695964466 0.0 14 1.4864937180775474E-4 0.0 0.0 11.493346454117885 0.0 15 1.4864937180775474E-4 0.0 0.0 11.91402417633383 0.0 16 1.4864937180775474E-4 0.0 0.0 12.424560443807565 0.0 17 1.4864937180775474E-4 0.0 0.0 13.074455497351067 0.0 18 1.4864937180775474E-4 0.0 0.0 13.70993156182922 0.0 19 1.4864937180775474E-4 0.0 0.0 14.690125519529554 0.0 20 1.4864937180775474E-4 0.0 0.0 15.263837770021583 0.0 21 1.4864937180775474E-4 0.0 0.0 15.934915359047693 0.0 22 1.4864937180775474E-4 0.0 0.0 16.704547481582342 0.0 23 1.4864937180775474E-4 0.0 0.0 17.393908943340804 0.0 24 1.4864937180775474E-4 0.0 0.0 17.942945398112748 0.0 25 1.4864937180775474E-4 0.0 0.0 18.429251817981818 0.0 26 1.4864937180775474E-4 0.0 0.0 18.864348529263115 0.0 27 1.4864937180775474E-4 0.0 0.0 19.399932215886455 0.0 28 1.4864937180775474E-4 0.0 0.0 19.888096752903124 0.0 29 1.4864937180775474E-4 0.0 0.0 20.4435251306628 0.0 30 1.4864937180775474E-4 0.0 0.0 21.053359178504113 0.0 31 1.4864937180775474E-4 0.0 0.0 21.528888518917118 0.0 32 1.4864937180775474E-4 0.0 0.0 22.00597867773411 0.0 33 1.4864937180775474E-4 0.0 0.0 22.48083909597398 0.0 34 1.4864937180775474E-4 0.0 0.0 23.014564665449726 0.0 35 2.229740577116321E-4 0.0 0.0 23.599797242256855 0.0 36 2.229740577116321E-4 0.0 0.0 24.089745571735214 0.0 37 2.229740577116321E-4 0.0 0.0 24.617971114454072 0.0 38 2.972987436155095E-4 0.0 0.0 25.245717411598218 0.0 39 2.972987436155095E-4 0.0 0.0 26.280391364066094 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATCG 25 3.8911618E-5 45.0 40 CGATCGA 75 0.0 45.0 41 TAGGGCG 590 0.0 43.09322 5 AATACGG 710 0.0 42.781693 5 CGTTAGG 475 0.0 42.631577 2 TACGGCT 2135 0.0 42.470726 7 CGATGAA 2660 0.0 41.954887 19 CCGATGA 2655 0.0 41.779663 18 ACGGCTG 2195 0.0 41.51481 8 TATGGGA 2690 0.0 41.403343 4 TAATACG 740 0.0 41.351353 4 TTATGGG 3490 0.0 41.196274 3 TCGTTAG 235 0.0 41.17021 1 TTAGGGA 3340 0.0 41.160183 4 CCGATCG 55 6.184564E-11 40.909092 40 TAGGGAT 1860 0.0 40.76613 5 GATGAAT 2905 0.0 40.585197 20 TAGGGAG 1500 0.0 40.5 5 TAACGCG 45 1.9286745E-8 40.000004 26 AACGCGA 45 1.9286745E-8 40.000004 27 >>END_MODULE