SRR2934180.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343823
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	11818	3.4372336929175766	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	9980	2.902656308623914	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	6728	1.956820806054278	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCTCGGT	4210	1.224467240411491	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	2636	0.7666735500533705	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	2487	0.7233372985518712	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	2483	0.7221739092498175	No Hit
GAATCAGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	2226	0.647426146592869	No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	2129	0.6192139560180675	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTA	1881	0.5470838192907397	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	1601	0.46564656814698263	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	1440	0.4188201487393223	No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1144	0.33272934038735047	No Hit
GAATATGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	1058	0.3077164703931965	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	1055	0.30684392841665625	No Hit
GCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1001	0.2911381728389317	No Hit
CCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	744	0.21639041018198316	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	734	0.213481936926849	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGT	696	0.2024297385573391	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATG	597	0.1736358533315107	No Hit
GCTGACTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	596	0.17334500600599728	No Hit
CTGTCACTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	570	0.1657829755426484	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATCTCGG	569	0.165492128217135	No Hit
GAACTATCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	491	0.14280603682708834	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTAT	478	0.1390250215954139	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	428	0.12448265531974301	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	426	0.12390096066871618	No Hit
GAATACTACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	411	0.11953825078601489	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	403	0.11721147218190756	No Hit
TCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	380	0.11052198369509894	No Hit
GAATAATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCTCGGT	359	0.10441418985931714	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCC	35	1.2086093E-7	45.000004	4
TCCGGAC	20	7.0263806E-4	45.000004	15
GTGCAAG	20	7.0263806E-4	45.000004	1
TTCTACG	25	3.8850827E-5	45.000004	1
CGGGTCC	30	2.1610467E-6	45.000004	6
ACTATCA	20	7.0263806E-4	45.000004	12
TCGTTCC	20	7.0263806E-4	45.000004	44
TCGTTAG	65	0.0	45.000004	1
ACAGTCC	20	7.0263806E-4	45.000004	17
ACACGCG	20	7.0263806E-4	45.000004	36
CACGGTG	20	7.0263806E-4	45.000004	21
CACGGGC	40	6.7902874E-9	45.000004	4
CCGATCG	55	1.8189894E-12	45.000004	40
CCCTACA	40	6.7902874E-9	45.000004	27
GGCACGG	20	7.0263806E-4	45.000004	2
GGCACGA	20	7.0263806E-4	45.000004	8
TCTTACG	30	2.1610467E-6	45.000004	1
TTACTAG	20	7.0263806E-4	45.000004	1
CGATTGG	30	2.1610467E-6	45.000004	10
TTCCGAT	20	7.0263806E-4	45.000004	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content