##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343823
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.593226747483442	31.0	31.0	34.0	30.0	34.0
2	31.603944471428612	31.0	31.0	34.0	30.0	34.0
3	31.708428464646055	31.0	31.0	34.0	30.0	34.0
4	35.50104559613522	37.0	35.0	37.0	33.0	37.0
5	30.37293316619307	35.0	35.0	37.0	0.0	37.0
6	24.599506141241278	35.0	17.0	37.0	0.0	37.0
7	30.66377176628672	35.0	26.0	37.0	17.0	37.0
8	33.355255465748364	35.0	32.0	37.0	28.0	37.0
9	36.47370303906371	37.0	35.0	39.0	32.0	39.0
10	36.77611736271279	38.0	35.0	39.0	33.0	39.0
11	36.95936281167926	39.0	37.0	39.0	33.0	39.0
12	36.92116001547308	39.0	35.0	39.0	33.0	39.0
13	36.67430625641682	39.0	35.0	39.0	32.0	39.0
14	37.741343656474406	40.0	36.0	41.0	33.0	41.0
15	37.4591693982078	40.0	36.0	41.0	32.0	41.0
16	37.943223693586525	40.0	36.0	41.0	33.0	41.0
17	37.79279163988448	40.0	36.0	41.0	33.0	41.0
18	37.90627735782655	40.0	36.0	41.0	33.0	41.0
19	37.940576982924355	40.0	37.0	41.0	33.0	41.0
20	37.59283701206726	40.0	36.0	41.0	32.0	41.0
21	37.73332499571	39.0	36.0	41.0	33.0	41.0
22	37.8227460059391	39.0	36.0	41.0	33.0	41.0
23	37.59808680629277	39.0	36.0	41.0	33.0	41.0
24	37.58712186212092	39.0	36.0	41.0	33.0	41.0
25	37.462842799929035	39.0	36.0	41.0	33.0	41.0
26	37.32631033991327	39.0	35.0	41.0	32.0	41.0
27	36.39628529795854	39.0	35.0	40.0	30.0	41.0
28	36.82668989567306	39.0	35.0	40.0	31.0	41.0
29	37.06071147072767	39.0	35.0	40.0	31.0	41.0
30	37.13376359347688	39.0	35.0	40.0	32.0	41.0
31	37.29783638674551	39.0	35.0	40.0	32.0	41.0
32	36.969059661511885	39.0	35.0	40.0	31.0	41.0
33	36.001887599142584	39.0	35.0	40.0	30.0	41.0
34	36.22766074404563	39.0	35.0	40.0	30.0	41.0
35	36.48280946882553	39.0	35.0	40.0	30.0	41.0
36	36.574208240868124	39.0	35.0	40.0	30.0	41.0
37	36.49659563205486	39.0	35.0	40.0	31.0	41.0
38	36.56395296417052	39.0	35.0	40.0	31.0	41.0
39	36.65376080134255	39.0	35.0	40.0	31.0	41.0
40	36.56687888826518	39.0	35.0	40.0	31.0	41.0
41	36.51196691320825	39.0	35.0	40.0	30.0	41.0
42	36.52642493375952	39.0	35.0	40.0	31.0	41.0
43	36.34061130290876	39.0	35.0	40.0	31.0	41.0
44	36.418119206684835	39.0	35.0	40.0	31.0	41.0
45	36.45976854369836	39.0	35.0	40.0	31.0	41.0
46	36.39989180479491	39.0	35.0	40.0	31.0	41.0
47	36.1705703225206	38.0	35.0	40.0	31.0	41.0
48	36.23764262425725	39.0	35.0	40.0	31.0	41.0
49	36.383200076783694	39.0	35.0	40.0	31.0	41.0
50	36.22176526875747	39.0	35.0	40.0	31.0	41.0
51	35.521628861361805	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	3.0
12	4.0
13	3.0
14	0.0
15	5.0
16	9.0
17	21.0
18	36.0
19	145.0
20	398.0
21	839.0
22	1547.0
23	2008.0
24	2348.0
25	2482.0
26	2520.0
27	2684.0
28	3122.0
29	4300.0
30	6211.0
31	9061.0
32	12714.0
33	19466.0
34	26210.0
35	34232.0
36	40943.0
37	57090.0
38	64300.0
39	51087.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.88651137358467	3.903171108390073	40.58192732888725	15.628390189138017
2	16.190598069355453	23.831448157918462	41.42480287822513	18.55315089450095
3	16.99711770300416	24.075759911349735	41.90266503404368	17.024457351602422
4	16.267381763290995	3.362485930260628	60.15682487791684	20.21330742853154
5	26.56657640704665	18.528138024506795	35.482501170660484	19.42278439778607
6	13.095400831241658	39.027348374018025	34.49100263798524	13.386248156755073
7	66.04415644095944	1.8367008606172364	26.01163970996705	6.10750298845627
8	62.770669792305924	16.28308751886872	11.99977895603261	8.946463732792745
9	59.27439409230912	4.479921354883181	12.92380090918874	23.321883643618953
10	42.05768665854233	19.948636362314332	20.919775582203634	17.073901396939707
11	33.425628884629596	19.784598470724763	26.54127268972698	20.248499954918664
12	24.594922387391186	17.005843122769566	31.0761641891322	27.323070300707048
13	24.321525901408574	22.14656960121923	37.06238384284937	16.46952065452282
14	18.61946408471801	32.21134130061107	26.240536555146104	22.928658059524814
15	12.56809463008583	21.774285024562058	42.58237523376854	23.075245111583577
16	13.674477856338871	23.188384721208298	29.58760757715452	33.54952984529831
17	14.200620668192645	26.283581959322095	32.92973419462921	26.58606317785605
18	15.315147619560065	22.35568882826338	33.210692711075176	29.11847084110138
19	17.51540763706907	24.713878943526176	28.8869563699927	28.88375704941205
20	21.998819159858414	23.545836084264288	36.68689994561155	17.768444810265745
21	20.895053559534993	31.357995247554697	28.152566873071315	19.594384319838987
22	16.840932689203456	21.603266797160167	33.63125794376758	27.924542569868798
23	19.56733551856624	29.261567725253983	27.173283928067637	23.99781282811214
24	21.650674911218854	22.647408695753338	27.31783504884781	28.38408134418
25	15.649912891226009	35.08549457133467	23.800909188739556	25.463683348699767
26	15.87386533187134	26.518586598336935	30.114332083659324	27.4932159861324
27	20.504154754044958	32.60805705261137	24.630405761103823	22.257382432239844
28	13.086093716825225	27.65318201516478	35.73495664920613	23.52576761880386
29	18.222457485392194	24.767394851420644	29.41862528103123	27.591522382155937
30	16.07222320787149	32.384104611966045	31.371083377202808	20.172588802959663
31	21.09486567216271	23.271857903630647	25.31767799129203	30.31559843291461
32	25.34240001396067	29.90026845208145	21.083231779142174	23.674099754815707
33	21.56196647693726	24.17057613946711	25.500039264388946	28.767418119206685
34	21.566329186819964	24.964880185444255	25.018396093338723	28.450394534397063
35	23.13661389726691	25.396788463831683	24.153125299936303	27.313472338965106
36	22.675330039002624	28.656023593535046	27.06159855507049	21.60704781239184
37	17.091061389144997	26.807107145246245	30.46887497346018	25.63295649214858
38	20.208363023997812	21.981659167653124	30.93335815230511	26.876619656043953
39	21.688194216210086	21.91505513011055	26.95456673928155	29.442183914397813
40	22.893465533137693	19.464957259985518	32.405918161379546	25.23565904549725
41	22.59389278785887	22.909171288715417	23.050232241589423	31.44670368183629
42	26.604968254014423	19.528943671598466	29.564339791113454	24.30174828327366
43	29.057683750069074	21.688194216210086	24.406744167784005	24.847377865936835
44	20.59955267681336	22.110213685530056	30.7163860474721	26.573847590184485
45	19.071731675891375	19.32767732234318	28.865142820579194	32.73544818118625
46	27.805004319082784	23.486794077185063	25.734753056078276	22.97344854765388
47	15.531538029742048	21.784464680955026	39.707931115719425	22.9760661735835
48	20.722871942831052	22.992353623812253	27.3067828504783	28.9779915828784
49	21.3243442119928	16.648391759713572	37.54577209785268	24.481491930440953
50	24.88518801825358	17.64425300227152	30.02387856542465	27.44668041405025
51	19.765984241891903	18.64156848145703	26.55057980414341	35.041867472507654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	195.5
2	316.0
3	590.5
4	865.0
5	645.0
6	425.0
7	458.5
8	492.0
9	523.0
10	554.0
11	704.0
12	854.0
13	871.0
14	888.0
15	833.0
16	778.0
17	776.0
18	774.0
19	744.0
20	714.0
21	807.5
22	901.0
23	916.0
24	931.0
25	1165.5
26	1803.5
27	2207.0
28	2482.0
29	2757.0
30	3560.0
31	4363.0
32	4321.0
33	4279.0
34	5014.0
35	5749.0
36	6534.5
37	7320.0
38	8626.5
39	9933.0
40	11986.5
41	14040.0
42	16299.5
43	18559.0
44	22956.0
45	27353.0
46	50563.5
47	73774.0
48	60682.0
49	47590.0
50	41889.5
51	36189.0
52	28479.0
53	20769.0
54	17857.0
55	14945.0
56	13430.0
57	11915.0
58	10684.0
59	9453.0
60	8520.0
61	7587.0
62	6360.0
63	5133.0
64	4353.0
65	3573.0
66	2891.0
67	2209.0
68	1850.0
69	1491.0
70	1251.5
71	1012.0
72	882.0
73	752.0
74	629.0
75	344.5
76	183.0
77	143.0
78	103.0
79	79.5
80	56.0
81	52.0
82	48.0
83	28.0
84	8.0
85	4.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	343823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.09901075759784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.23175917126424	26.435940621212822
2	11.018756882488118	7.955324464725876
3	4.401118703623279	4.766280942826854
4	2.4350097095302736	3.5160576679675413
5	1.6057540753381676	2.8983066819844536
6	1.1868244503696512	2.5705913160764506
7	0.9154027552560019	2.313159373666478
8	0.7047637112696055	2.035301823574885
9	0.5547923214215805	1.802470858130728
>10	3.7901161641733996	22.31976193811402
>50	0.07948999663563927	1.9364756757806916
>100	0.057363915097884	4.1791310528191
>500	0.0057363915097884	1.3329910472147564
>1k	0.010653298518178459	7.49947981312501
>5k	0.001638969002796686	4.942657438274335
>10k+	8.19484501398343E-4	3.4960692845059906
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	11818	3.4372336929175766	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	9980	2.902656308623914	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	6728	1.956820806054278	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCTCGGT	4210	1.224467240411491	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	2636	0.7666735500533705	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	2487	0.7233372985518712	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	2483	0.7221739092498175	No Hit
GAATCAGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	2226	0.647426146592869	No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	2129	0.6192139560180675	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTA	1881	0.5470838192907397	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	1601	0.46564656814698263	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	1440	0.4188201487393223	No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1144	0.33272934038735047	No Hit
GAATATGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	1058	0.3077164703931965	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	1055	0.30684392841665625	No Hit
GCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1001	0.2911381728389317	No Hit
CCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	744	0.21639041018198316	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	734	0.213481936926849	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGT	696	0.2024297385573391	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATG	597	0.1736358533315107	No Hit
GCTGACTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	596	0.17334500600599728	No Hit
CTGTCACTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	570	0.1657829755426484	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATCTCGG	569	0.165492128217135	No Hit
GAACTATCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	491	0.14280603682708834	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTAT	478	0.1390250215954139	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	428	0.12448265531974301	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	426	0.12390096066871618	No Hit
GAATACTACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	411	0.11953825078601489	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	403	0.11721147218190756	No Hit
TCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	380	0.11052198369509894	No Hit
GAATAATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCTCGGT	359	0.10441418985931714	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5008390945341062	0.0
2	0.0	0.0	0.0	2.3561541839842013	0.0
3	0.0	0.0	0.0	3.0335376051049523	0.0
4	0.0	0.0	0.0	4.111126946132167	0.0
5	0.0	0.0	0.0	7.21621299331342	0.0
6	0.0	0.0	0.0	7.996556367665921	0.0
7	0.0	0.0	0.0	9.736114221561676	0.0
8	0.0	0.0	0.0	11.837486148396122	0.0
9	0.0	0.0	0.0	12.423834356631174	0.0
10	0.0	0.0	0.0	16.98839228323876	0.0
11	0.0	0.0	0.0	19.086274042167044	0.0
12	0.0	0.0	0.0	25.245547854564702	0.0
13	0.0	0.0	0.0	26.106746785409936	0.0
14	0.0	0.0	0.0	26.510152025897046	0.0
15	0.0	0.0	0.0	27.507176657757043	0.0
16	0.0	0.0	0.0	28.558008044837024	0.0
17	0.0	0.0	0.0	29.739429881072528	0.0
18	0.0	0.0	0.0	30.846394801976597	0.0
19	0.0	0.0	0.0	33.24414015350922	0.0
20	0.0	0.0	0.0	34.172815663873564	0.0
21	0.0	0.0	0.0	35.18990876119398	0.0
22	0.0	0.0	0.0	36.244812010831154	0.0
23	0.0	0.0	0.0	37.2016997117703	0.0
24	0.0	0.0	0.0	37.97360851368291	0.0
25	0.0	0.0	0.0	38.61754449236962	0.0
26	0.0	0.0	0.0	39.200111685372995	0.0
27	0.0	0.0	0.0	39.84404766405971	0.0
28	0.0	0.0	0.0	40.38386030021261	0.0
29	0.0	0.0	0.0	40.992894599837705	0.0
30	0.0	0.0	0.0	41.646428540266356	0.0
31	0.0	0.0	0.0	42.242374710243354	0.0
32	0.0	0.0	0.0	42.828722918478405	0.0
33	0.0	0.0	0.0	43.32694438708289	0.0
34	0.0	0.0	0.0	43.87722752695428	0.0
35	0.0	0.0	0.0	44.46619336111895	0.0
36	0.0	0.0	0.0	45.00746023389942	0.0
37	0.0	0.0	0.0	45.57344912934853	0.0
38	0.0	0.0	0.0	46.14147395607624	0.0
39	0.0	0.0	0.0	46.68884862269249	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCC	35	1.2086093E-7	45.000004	4
TCCGGAC	20	7.0263806E-4	45.000004	15
GTGCAAG	20	7.0263806E-4	45.000004	1
TTCTACG	25	3.8850827E-5	45.000004	1
CGGGTCC	30	2.1610467E-6	45.000004	6
ACTATCA	20	7.0263806E-4	45.000004	12
TCGTTCC	20	7.0263806E-4	45.000004	44
TCGTTAG	65	0.0	45.000004	1
ACAGTCC	20	7.0263806E-4	45.000004	17
ACACGCG	20	7.0263806E-4	45.000004	36
CACGGTG	20	7.0263806E-4	45.000004	21
CACGGGC	40	6.7902874E-9	45.000004	4
CCGATCG	55	1.8189894E-12	45.000004	40
CCCTACA	40	6.7902874E-9	45.000004	27
GGCACGG	20	7.0263806E-4	45.000004	2
GGCACGA	20	7.0263806E-4	45.000004	8
TCTTACG	30	2.1610467E-6	45.000004	1
TTACTAG	20	7.0263806E-4	45.000004	1
CGATTGG	30	2.1610467E-6	45.000004	10
TTCCGAT	20	7.0263806E-4	45.000004	13
>>END_MODULE